Interação de Paracoccidioides brasiliensis com células endoteliais

Detalhes bibliográficos
Autor(a) principal: Silva, Juliana Leal Monteiro da [UNESP]
Data de Publicação: 2005
Outros Autores: Andreotti, P. F. [UNESP], Mendes-Giannini, Maria José Soares [UNESP]
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/415
http://hdl.handle.net/11449/68630
Resumo: Paracoccidioidomycosis has a variety of clinical manifestations and Paracoccidioides brasiliensis, the causative agent, may infect many tissues, most importantly the lungs. Migration of pathogenic yeasts to the endothelial cell layer is considered a prerequisite for multiple organ invasion and dissemination of the fungus. In this study of the adhesion of P. brasiliensis to endothelial cells in vitro, we investigated whether this adhesion could represent a mechanism of dissemination. To this end, as well as using conventional optical microscopy, an alternative in vivo technique was developed, to detect the presence of fungal cells in umbilical cords embedded in paraffin wax. An experiment on the migration of P. brasiliensis through an endothelial cell monolayer was carried out, and the migration of yeast cells was greater, and took less time, in control wells with no cells. The fungus crossed the monolayer, but, compared to control wells, the migration-rate was about 30% lower. This shows that the monolayer only partially blocked migration of the fungus. In these experiments, we had great difficulty finding P. brasiliensis adhered to the cell monolayer, when it was examined at different times, suggesting that migration of the fungus across the endothelial layer is very fast, and cannot normally be observed in cell culture in vitro. Thus, P. brasiliensis can cross the endothelium rapidly and probably invades deeper tissue.
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spelling Interação de Paracoccidioides brasiliensis com células endoteliaisInteraction of Paracoccidioides brasiliensis with endothelial cellsEndothelial cellsMigrationParacoccidioides brasiliensiscell adhesionendothelium cellfungal cellin vitro studymicroscopymigrationSouth American blastomycosisParacoccidioidomycosis has a variety of clinical manifestations and Paracoccidioides brasiliensis, the causative agent, may infect many tissues, most importantly the lungs. Migration of pathogenic yeasts to the endothelial cell layer is considered a prerequisite for multiple organ invasion and dissemination of the fungus. In this study of the adhesion of P. brasiliensis to endothelial cells in vitro, we investigated whether this adhesion could represent a mechanism of dissemination. To this end, as well as using conventional optical microscopy, an alternative in vivo technique was developed, to detect the presence of fungal cells in umbilical cords embedded in paraffin wax. An experiment on the migration of P. brasiliensis through an endothelial cell monolayer was carried out, and the migration of yeast cells was greater, and took less time, in control wells with no cells. The fungus crossed the monolayer, but, compared to control wells, the migration-rate was about 30% lower. This shows that the monolayer only partially blocked migration of the fungus. In these experiments, we had great difficulty finding P. brasiliensis adhered to the cell monolayer, when it was examined at different times, suggesting that migration of the fungus across the endothelial layer is very fast, and cannot normally be observed in cell culture in vitro. Thus, P. brasiliensis can cross the endothelium rapidly and probably invades deeper tissue.A paracoccidioidomicose apresenta um amplo espectro de manifestações clínicas e Paracoccidioides brasiliensis, seu agente etiológico, pode atingir vários tecidos com ênfase ao pulmão. A migração de fungos patogênicos através da camada de células endoteliais é considerada pré-requisito para a invasão de múltiplos órgãos e sua disseminação. No presente estudo verificou-se a adesão de P. brasiliensis às células endoteliais in vitro e se esta adesão poderia representar um mecanismo para a disseminação do fungo. Para tanto, além da técnica convencional de microscopia ótica, uma outra metodologia foi desenvolvida, emblocando os cordões umbilicais em parafina, no intuito de detectar o fungo presente no material (in vivo). Experimento de migração de P. brasiliensis através da monocamada de células endoteliais também foi realizado, e nos poços sem células, a migração de células leveduriformes foi maior em menor período de tempo. Os fungos conseguiram passar através da monocamada, quando comparados com o controle sem as células, mas com redução em torno de 30%. Isso mostra que a monocamada foi parcialmente impediente para o fungo, mas que este foi capaz de migrar através dessas células. Em nossos experimentos com estas células, houve grande dificuldade de se encontrar P. brasiliensis aderido ao tapete celular nos períodos de tempo padronizados. Sugere-se com esses resultados que o fungo atravessa as células endoteliais de uma maneira muito rápida, que não pode ser detectada através do cultivo in vitro. Portanto, P. brasiliensis teria capacidade de atravessar rapidamente as células endoteliais e provavelmente alcançar tecidos mais profundos.Departamento de Análises Clínicas Faculdade de Ciências Farmacêuticas UNESP, Araraquara, SPDepartamento de Análises Clínicas Faculdade de Ciências Farmacêuticas UNESP, Rua Expedicionarios do Brasil, 1621, 14801-902, Araraquara, SPDepartamento de Análises Clínicas Faculdade de Ciências Farmacêuticas UNESP, Araraquara, SPDepartamento de Análises Clínicas Faculdade de Ciências Farmacêuticas UNESP, Rua Expedicionarios do Brasil, 1621, 14801-902, Araraquara, SPUniversidade Estadual Paulista (Unesp)Silva, Juliana Leal Monteiro da [UNESP]Andreotti, P. F. [UNESP]Mendes-Giannini, Maria José Soares [UNESP]2014-05-27T11:21:44Z2014-05-27T11:21:44Z2005-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article149-156application/pdfhttp://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/415Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 26, n. 2, p. 149-156, 2005.1808-4532http://hdl.handle.net/11449/686302-s2.0-337456492272-s2.0-33745649227.pdf0000-0002-8059-0826Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista de Ciências Farmacêuticas Básica e Aplicada0,131info:eu-repo/semantics/openAccess2024-06-21T15:18:33Zoai:repositorio.unesp.br:11449/68630Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:25:27.276667Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Interação de Paracoccidioides brasiliensis com células endoteliais
Interaction of Paracoccidioides brasiliensis with endothelial cells
title Interação de Paracoccidioides brasiliensis com células endoteliais
spellingShingle Interação de Paracoccidioides brasiliensis com células endoteliais
Silva, Juliana Leal Monteiro da [UNESP]
Endothelial cells
Migration
Paracoccidioides brasiliensis
cell adhesion
endothelium cell
fungal cell
in vitro study
microscopy
migration
South American blastomycosis
title_short Interação de Paracoccidioides brasiliensis com células endoteliais
title_full Interação de Paracoccidioides brasiliensis com células endoteliais
title_fullStr Interação de Paracoccidioides brasiliensis com células endoteliais
title_full_unstemmed Interação de Paracoccidioides brasiliensis com células endoteliais
title_sort Interação de Paracoccidioides brasiliensis com células endoteliais
author Silva, Juliana Leal Monteiro da [UNESP]
author_facet Silva, Juliana Leal Monteiro da [UNESP]
Andreotti, P. F. [UNESP]
Mendes-Giannini, Maria José Soares [UNESP]
author_role author
author2 Andreotti, P. F. [UNESP]
Mendes-Giannini, Maria José Soares [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Silva, Juliana Leal Monteiro da [UNESP]
Andreotti, P. F. [UNESP]
Mendes-Giannini, Maria José Soares [UNESP]
dc.subject.por.fl_str_mv Endothelial cells
Migration
Paracoccidioides brasiliensis
cell adhesion
endothelium cell
fungal cell
in vitro study
microscopy
migration
South American blastomycosis
topic Endothelial cells
Migration
Paracoccidioides brasiliensis
cell adhesion
endothelium cell
fungal cell
in vitro study
microscopy
migration
South American blastomycosis
description Paracoccidioidomycosis has a variety of clinical manifestations and Paracoccidioides brasiliensis, the causative agent, may infect many tissues, most importantly the lungs. Migration of pathogenic yeasts to the endothelial cell layer is considered a prerequisite for multiple organ invasion and dissemination of the fungus. In this study of the adhesion of P. brasiliensis to endothelial cells in vitro, we investigated whether this adhesion could represent a mechanism of dissemination. To this end, as well as using conventional optical microscopy, an alternative in vivo technique was developed, to detect the presence of fungal cells in umbilical cords embedded in paraffin wax. An experiment on the migration of P. brasiliensis through an endothelial cell monolayer was carried out, and the migration of yeast cells was greater, and took less time, in control wells with no cells. The fungus crossed the monolayer, but, compared to control wells, the migration-rate was about 30% lower. This shows that the monolayer only partially blocked migration of the fungus. In these experiments, we had great difficulty finding P. brasiliensis adhered to the cell monolayer, when it was examined at different times, suggesting that migration of the fungus across the endothelial layer is very fast, and cannot normally be observed in cell culture in vitro. Thus, P. brasiliensis can cross the endothelium rapidly and probably invades deeper tissue.
publishDate 2005
dc.date.none.fl_str_mv 2005-12-01
2014-05-27T11:21:44Z
2014-05-27T11:21:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/415
Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 26, n. 2, p. 149-156, 2005.
1808-4532
http://hdl.handle.net/11449/68630
2-s2.0-33745649227
2-s2.0-33745649227.pdf
0000-0002-8059-0826
url http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/415
http://hdl.handle.net/11449/68630
identifier_str_mv Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 26, n. 2, p. 149-156, 2005.
1808-4532
2-s2.0-33745649227
2-s2.0-33745649227.pdf
0000-0002-8059-0826
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Revista de Ciências Farmacêuticas Básica e Aplicada
0,131
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 149-156
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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