Use of EP(Peroxidase) allele in soybean varietal characterization

Detalhes bibliográficos
Autor(a) principal: Valário, Bárbara Panoff [UNESP]
Data de Publicação: 2014
Outros Autores: Cavariani, Cláudio [UNESP], França-neto, José De Barros, Vieira, Elisa Serra Negra, Bravo, Juliana Pereira [UNESP], Silva, Edvaldo Aparecido Amaral da [UNESP][
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/2317-1545v36n4996
http://hdl.handle.net/11449/114443
Resumo: The aim of this work was to evaluate the use of the molecular biology technique of PCR (Polimerase Chain Reaction) in the characterization of soybean cultivars. The study was performed at the Department of Plant Production, Faculty of Agricultural Sciences/ UNESP and Institute of Bioscience, Botucatu-SP. Fourteen commercial soybean cultivars were used, of which six were selected as positive reaction to peroxidase (BRS 320, BRS 284, BRS 232, BRS 7860RR, BRSMG 760SRR, BRS295RR), four as negative reaction (BRS 326, BRS 8160RR, BRSMG 800A (NutriSoy), BRS Valiosa RR) and four as double reaction (BRSGO 8060, BRS 270RR, FTS Campo Mourão and BRS 239). Thus, the results attained by the traditional biochemical colorimetric test for the 14 cultivars were compared with the conventional PCR assay. For PCR analysis, DNA was extracted from whole seeds and the primers were tested, and subsequently PCR and agarose gel electrophoresis were performed. The combination of primers prx9 + prx10 confirmed the use of the PCR reaction to characterize soybean cultivars considered doubtful by conventional colorimetric text.
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spelling Use of EP(Peroxidase) allele in soybean varietal characterizationUso do alelo EP (Peroxidase) na caracterização varietal de sojaGlycine max (L.) MerrillPCRDNApureza genéticaGlycine max (L.) MerrillPCRDNAgenetic purityThe aim of this work was to evaluate the use of the molecular biology technique of PCR (Polimerase Chain Reaction) in the characterization of soybean cultivars. The study was performed at the Department of Plant Production, Faculty of Agricultural Sciences/ UNESP and Institute of Bioscience, Botucatu-SP. Fourteen commercial soybean cultivars were used, of which six were selected as positive reaction to peroxidase (BRS 320, BRS 284, BRS 232, BRS 7860RR, BRSMG 760SRR, BRS295RR), four as negative reaction (BRS 326, BRS 8160RR, BRSMG 800A (NutriSoy), BRS Valiosa RR) and four as double reaction (BRSGO 8060, BRS 270RR, FTS Campo Mourão and BRS 239). Thus, the results attained by the traditional biochemical colorimetric test for the 14 cultivars were compared with the conventional PCR assay. For PCR analysis, DNA was extracted from whole seeds and the primers were tested, and subsequently PCR and agarose gel electrophoresis were performed. The combination of primers prx9 + prx10 confirmed the use of the PCR reaction to characterize soybean cultivars considered doubtful by conventional colorimetric text.O objetivo geral deste trabalho foi avaliar o uso da técnica de PCR (Polimerase Chain Reaction) na caracterização de cultivares de soja. O estudo foi realizado no Departamento de Produção Vegetal da Faculdade de Ciências Agronômicas / UNESP e do Instituto de Biociências de Botucatu-SP. Foram utilizadas quatorze cultivares de soja comerciais, das quais seis foram selecionadas como reação positiva à peroxidase (BRS 320, BRS 284, BRS 232, BRS 7860RR, BRSMG 760SRR, BRS295RR), quatro como reação negativa (BRS 326, BRS 8160RR, BRSMG 800A (NutriSoy), BRS Valiosa RR) e quatro como reação positiva e negativa (BRS 8060, BRS 270RR, FTS Campo Mourão e BRS 239). Assim, as 14 cultivares foram submetidas ao ensaio bioquímico colorimétrico tradicional e os resultados obtidos foram comparados com o ensaio de PCR convencional. Para a análise de PCR, o DNA foi extraído de sementes inteiras, sendo que os primers foram testados por PCR e visualizados por eletroforese em gel de agarose. A combinação dos primers prx9 + prx10 confirmou a utilização da reação de PCR para caracterizar as cultivares de soja considerada duvidosa por teste convencional colorimétrico.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)UNESP Departamento de Produção e Melhoramento VegetalEmbrapa SojaEmbrapa FlorestasUNESP Departamento de Produção e Melhoramento VegetalABRATES - Associação Brasileira de Tecnologia de SementesUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Valário, Bárbara Panoff [UNESP]Cavariani, Cláudio [UNESP]França-neto, José De BarrosVieira, Elisa Serra NegraBravo, Juliana Pereira [UNESP]Silva, Edvaldo Aparecido Amaral da [UNESP][2015-02-02T12:39:32Z2015-02-02T12:39:32Z2014-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article465-470application/pdfhttp://dx.doi.org/10.1590/2317-1545v36n4996Journal of Seed Science. ABRATES - Associação Brasileira de Tecnologia de Sementes, v. 36, n. 4, p. 465-470, 2014.2317-1537http://hdl.handle.net/11449/11444310.1590/2317-1545v36n4996S2317-15372014000400012S2317-15372014000400012.pdf99726437744913010000-0001-6454-1488SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Seed Science0,367info:eu-repo/semantics/openAccess2024-04-30T15:56:55Zoai:repositorio.unesp.br:11449/114443Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:57:01.329663Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Use of EP(Peroxidase) allele in soybean varietal characterization
Uso do alelo EP (Peroxidase) na caracterização varietal de soja
title Use of EP(Peroxidase) allele in soybean varietal characterization
spellingShingle Use of EP(Peroxidase) allele in soybean varietal characterization
Valário, Bárbara Panoff [UNESP]
Glycine max (L.) Merrill
PCR
DNA
pureza genética
Glycine max (L.) Merrill
PCR
DNA
genetic purity
title_short Use of EP(Peroxidase) allele in soybean varietal characterization
title_full Use of EP(Peroxidase) allele in soybean varietal characterization
title_fullStr Use of EP(Peroxidase) allele in soybean varietal characterization
title_full_unstemmed Use of EP(Peroxidase) allele in soybean varietal characterization
title_sort Use of EP(Peroxidase) allele in soybean varietal characterization
author Valário, Bárbara Panoff [UNESP]
author_facet Valário, Bárbara Panoff [UNESP]
Cavariani, Cláudio [UNESP]
França-neto, José De Barros
Vieira, Elisa Serra Negra
Bravo, Juliana Pereira [UNESP]
Silva, Edvaldo Aparecido Amaral da [UNESP][
author_role author
author2 Cavariani, Cláudio [UNESP]
França-neto, José De Barros
Vieira, Elisa Serra Negra
Bravo, Juliana Pereira [UNESP]
Silva, Edvaldo Aparecido Amaral da [UNESP][
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.author.fl_str_mv Valário, Bárbara Panoff [UNESP]
Cavariani, Cláudio [UNESP]
França-neto, José De Barros
Vieira, Elisa Serra Negra
Bravo, Juliana Pereira [UNESP]
Silva, Edvaldo Aparecido Amaral da [UNESP][
dc.subject.por.fl_str_mv Glycine max (L.) Merrill
PCR
DNA
pureza genética
Glycine max (L.) Merrill
PCR
DNA
genetic purity
topic Glycine max (L.) Merrill
PCR
DNA
pureza genética
Glycine max (L.) Merrill
PCR
DNA
genetic purity
description The aim of this work was to evaluate the use of the molecular biology technique of PCR (Polimerase Chain Reaction) in the characterization of soybean cultivars. The study was performed at the Department of Plant Production, Faculty of Agricultural Sciences/ UNESP and Institute of Bioscience, Botucatu-SP. Fourteen commercial soybean cultivars were used, of which six were selected as positive reaction to peroxidase (BRS 320, BRS 284, BRS 232, BRS 7860RR, BRSMG 760SRR, BRS295RR), four as negative reaction (BRS 326, BRS 8160RR, BRSMG 800A (NutriSoy), BRS Valiosa RR) and four as double reaction (BRSGO 8060, BRS 270RR, FTS Campo Mourão and BRS 239). Thus, the results attained by the traditional biochemical colorimetric test for the 14 cultivars were compared with the conventional PCR assay. For PCR analysis, DNA was extracted from whole seeds and the primers were tested, and subsequently PCR and agarose gel electrophoresis were performed. The combination of primers prx9 + prx10 confirmed the use of the PCR reaction to characterize soybean cultivars considered doubtful by conventional colorimetric text.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-01
2015-02-02T12:39:32Z
2015-02-02T12:39:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/2317-1545v36n4996
Journal of Seed Science. ABRATES - Associação Brasileira de Tecnologia de Sementes, v. 36, n. 4, p. 465-470, 2014.
2317-1537
http://hdl.handle.net/11449/114443
10.1590/2317-1545v36n4996
S2317-15372014000400012
S2317-15372014000400012.pdf
9972643774491301
0000-0001-6454-1488
url http://dx.doi.org/10.1590/2317-1545v36n4996
http://hdl.handle.net/11449/114443
identifier_str_mv Journal of Seed Science. ABRATES - Associação Brasileira de Tecnologia de Sementes, v. 36, n. 4, p. 465-470, 2014.
2317-1537
10.1590/2317-1545v36n4996
S2317-15372014000400012
S2317-15372014000400012.pdf
9972643774491301
0000-0001-6454-1488
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Seed Science
0,367
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 465-470
application/pdf
dc.publisher.none.fl_str_mv ABRATES - Associação Brasileira de Tecnologia de Sementes
publisher.none.fl_str_mv ABRATES - Associação Brasileira de Tecnologia de Sementes
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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