Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats

Detalhes bibliográficos
Autor(a) principal: Florencio-Silva, Rinaldo
Data de Publicação: 2018
Outros Autores: Sasso, Gisela R.S., Sasso-Cerri, Estela [UNESP], Simões, Manuel J., Cerri, Paulo S. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.biopha.2017.12.089
http://hdl.handle.net/11449/177071
Resumo: Estrogen maintains osteocyte viability, whereas its deficiency induces osteocyte apoptosis. As autophagy is important for osteocyte viability, we hypothesized whether the anti-apoptotic effect of estrogen is related to autophagy in osteocytes. Thirty adult female rats were sham-operated (SHAM) or ovariectomized (OVX). After three weeks, twelve rats of SHAM and OVX groups were killed before treatment (basal period), whereas the remaining rats received estrogen (OVXE) or vehicle (OVX) for 45 days. Fragments of maxilla containing alveolar process of the first molars were embedded in paraffin or Araldite. Paraffin-sections were stained with hematoxylin/eosin for histomorphometry, or subjected to the silver impregnation method for morphological analysis of osteocyte cytoplasmic processes. Autophagy was analyzed by immunohistochemical detections of beclin-1, MAP-LC3α and p62, whereas apoptosis was evaluated by immunohistochemical detections of cleaved caspase-3 and BAX, TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) method and by ultrastructural analysis. Araldite-semithin sections were subjected to the Sudan-black method for detection of lipids. OVX-basal group showed high frequency of caspase-3-, TUNEL- and p62-positive osteocytes accompanied with low frequency of beclin-1- and MAP-LC3α-positive osteocytes. At 45 days, OVXE group exhibited higher number of osteocytes, higher frequency of beclin-1- and MAP-LC3α-positive osteocytes, and lower frequency of caspase-3, BAX-, TUNEL- and p62-positive osteocytes than OVX group. Significant reduction in bone area was observed in the OVX compared to OVXE and SHAM groups. The highest frequency of Sudan-Black-positive osteocytes and osteocytes with scarce cytoplasmic processes, or showing apoptotic features were mainly observed in OVX groups. Our results indicate that estrogen deficiency decreases autophagy and increases apoptosis, whereas estrogen replacement enhances osteocyte viability by inhibiting apoptosis and maintaining autophagy in alveolar process osteocytes. These results suggest that the anti-apoptotic effect of estrogen may be, at least in part, related to autophagy regulation in osteocytes.
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spelling Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized ratsAlveolar processApoptosisAutophagyEstrogenOsteocytesEstrogen maintains osteocyte viability, whereas its deficiency induces osteocyte apoptosis. As autophagy is important for osteocyte viability, we hypothesized whether the anti-apoptotic effect of estrogen is related to autophagy in osteocytes. Thirty adult female rats were sham-operated (SHAM) or ovariectomized (OVX). After three weeks, twelve rats of SHAM and OVX groups were killed before treatment (basal period), whereas the remaining rats received estrogen (OVXE) or vehicle (OVX) for 45 days. Fragments of maxilla containing alveolar process of the first molars were embedded in paraffin or Araldite. Paraffin-sections were stained with hematoxylin/eosin for histomorphometry, or subjected to the silver impregnation method for morphological analysis of osteocyte cytoplasmic processes. Autophagy was analyzed by immunohistochemical detections of beclin-1, MAP-LC3α and p62, whereas apoptosis was evaluated by immunohistochemical detections of cleaved caspase-3 and BAX, TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) method and by ultrastructural analysis. Araldite-semithin sections were subjected to the Sudan-black method for detection of lipids. OVX-basal group showed high frequency of caspase-3-, TUNEL- and p62-positive osteocytes accompanied with low frequency of beclin-1- and MAP-LC3α-positive osteocytes. At 45 days, OVXE group exhibited higher number of osteocytes, higher frequency of beclin-1- and MAP-LC3α-positive osteocytes, and lower frequency of caspase-3, BAX-, TUNEL- and p62-positive osteocytes than OVX group. Significant reduction in bone area was observed in the OVX compared to OVXE and SHAM groups. The highest frequency of Sudan-Black-positive osteocytes and osteocytes with scarce cytoplasmic processes, or showing apoptotic features were mainly observed in OVX groups. Our results indicate that estrogen deficiency decreases autophagy and increases apoptosis, whereas estrogen replacement enhances osteocyte viability by inhibiting apoptosis and maintaining autophagy in alveolar process osteocytes. These results suggest that the anti-apoptotic effect of estrogen may be, at least in part, related to autophagy regulation in osteocytes.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UniversidadeFederal de São Paulo -UNIFESP Escola Paulista de Medicina –EPM Departamento de Morfologia e Genética Disciplina de Histologia e Biologia EstruturalUniversidade Federal de São Paulo- UNIFESP Escola Paulista deMedicina – EPM Departmento de GinecologiaSão Paulo State University (UNESP) School of Dentistry Araraquara– Laboratory of Histology and EmbryologySão Paulo State University (UNESP) School of Dentistry Araraquara– Laboratory of Histology and EmbryologyFAPESP: 2012/19428-8; 2012/22666-8Universidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (Unesp)Florencio-Silva, RinaldoSasso, Gisela R.S.Sasso-Cerri, Estela [UNESP]Simões, Manuel J.Cerri, Paulo S. [UNESP]2018-12-11T17:23:43Z2018-12-11T17:23:43Z2018-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article406-415application/pdfhttp://dx.doi.org/10.1016/j.biopha.2017.12.089Biomedicine and Pharmacotherapy, v. 98, p. 406-415.1950-60070753-3322http://hdl.handle.net/11449/17707110.1016/j.biopha.2017.12.0892-s2.0-850388390992-s2.0-85038839099.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomedicine and Pharmacotherapy0,951info:eu-repo/semantics/openAccess2024-08-16T14:07:08Zoai:repositorio.unesp.br:11449/177071Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:07:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats
title Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats
spellingShingle Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats
Florencio-Silva, Rinaldo
Alveolar process
Apoptosis
Autophagy
Estrogen
Osteocytes
title_short Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats
title_full Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats
title_fullStr Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats
title_full_unstemmed Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats
title_sort Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats
author Florencio-Silva, Rinaldo
author_facet Florencio-Silva, Rinaldo
Sasso, Gisela R.S.
Sasso-Cerri, Estela [UNESP]
Simões, Manuel J.
Cerri, Paulo S. [UNESP]
author_role author
author2 Sasso, Gisela R.S.
Sasso-Cerri, Estela [UNESP]
Simões, Manuel J.
Cerri, Paulo S. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Florencio-Silva, Rinaldo
Sasso, Gisela R.S.
Sasso-Cerri, Estela [UNESP]
Simões, Manuel J.
Cerri, Paulo S. [UNESP]
dc.subject.por.fl_str_mv Alveolar process
Apoptosis
Autophagy
Estrogen
Osteocytes
topic Alveolar process
Apoptosis
Autophagy
Estrogen
Osteocytes
description Estrogen maintains osteocyte viability, whereas its deficiency induces osteocyte apoptosis. As autophagy is important for osteocyte viability, we hypothesized whether the anti-apoptotic effect of estrogen is related to autophagy in osteocytes. Thirty adult female rats were sham-operated (SHAM) or ovariectomized (OVX). After three weeks, twelve rats of SHAM and OVX groups were killed before treatment (basal period), whereas the remaining rats received estrogen (OVXE) or vehicle (OVX) for 45 days. Fragments of maxilla containing alveolar process of the first molars were embedded in paraffin or Araldite. Paraffin-sections were stained with hematoxylin/eosin for histomorphometry, or subjected to the silver impregnation method for morphological analysis of osteocyte cytoplasmic processes. Autophagy was analyzed by immunohistochemical detections of beclin-1, MAP-LC3α and p62, whereas apoptosis was evaluated by immunohistochemical detections of cleaved caspase-3 and BAX, TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) method and by ultrastructural analysis. Araldite-semithin sections were subjected to the Sudan-black method for detection of lipids. OVX-basal group showed high frequency of caspase-3-, TUNEL- and p62-positive osteocytes accompanied with low frequency of beclin-1- and MAP-LC3α-positive osteocytes. At 45 days, OVXE group exhibited higher number of osteocytes, higher frequency of beclin-1- and MAP-LC3α-positive osteocytes, and lower frequency of caspase-3, BAX-, TUNEL- and p62-positive osteocytes than OVX group. Significant reduction in bone area was observed in the OVX compared to OVXE and SHAM groups. The highest frequency of Sudan-Black-positive osteocytes and osteocytes with scarce cytoplasmic processes, or showing apoptotic features were mainly observed in OVX groups. Our results indicate that estrogen deficiency decreases autophagy and increases apoptosis, whereas estrogen replacement enhances osteocyte viability by inhibiting apoptosis and maintaining autophagy in alveolar process osteocytes. These results suggest that the anti-apoptotic effect of estrogen may be, at least in part, related to autophagy regulation in osteocytes.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:23:43Z
2018-12-11T17:23:43Z
2018-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.biopha.2017.12.089
Biomedicine and Pharmacotherapy, v. 98, p. 406-415.
1950-6007
0753-3322
http://hdl.handle.net/11449/177071
10.1016/j.biopha.2017.12.089
2-s2.0-85038839099
2-s2.0-85038839099.pdf
url http://dx.doi.org/10.1016/j.biopha.2017.12.089
http://hdl.handle.net/11449/177071
identifier_str_mv Biomedicine and Pharmacotherapy, v. 98, p. 406-415.
1950-6007
0753-3322
10.1016/j.biopha.2017.12.089
2-s2.0-85038839099
2-s2.0-85038839099.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biomedicine and Pharmacotherapy
0,951
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 406-415
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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