Natural killer activity in a medium-term multi-organ bioassay for carcinogenesis
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/195683 |
Resumo: | Natural killer (NK) cell activity was evaluated after the initiation and promotion steps in a medium-term multi-organ bioassay for carcinogenesis. NK cell activity was assessed in vitro by Cr-51 release assay at the 4th and 30th weeks of the experiment. Male Wistar rats were sequentially initiated with N-diethylnitrosamine (DEN i.p.), N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN drinking water), N-methyl-N-nitrosourea (MNU i.p.), dihydroxy-di-N-propylnitrosamine (DHPN drinking water) and N,N'-dimethylhydrazine (DMH s.c.) at subcarcinogenic doses for 4 weeks (DMBDD initiation). One group was evaluated at the 4th week and the other was maintained without any further treatment until the 30th week. Two initiated groups were exposed through the diet to 2-acetylaminofluorene (2-AAF) or phenobarbital (PB), from the 6th until the 30th week, Five additional groups were studied to evaluate the effects of each initiator on NK activity. All groups submitted to initiation only, initiation plus promotion, or promotion only, developed significantly more preneoplastic lesions than the untreated control group. The main target organs for tumor development in the initiated animals n ere the liver and the colon, irrespective of treatment with 2-AAF or PB. NK cell activity was not affected bal exposure to genotoxic carcinogens after initiation, at the 4th week. Treatments only with PB or 2-AAF did not change NK cell activity, However, decreased NK cell activity was registered in the group only initiated with DMBDD and in the group given DMBDD+2-AAF. This late depression of NK cell activity at the 30th week could be related to the production of suppressing molecules by the tumor cells. |
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Repositório Institucional da UNESP |
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Natural killer activity in a medium-term multi-organ bioassay for carcinogenesisNK cell activityimmune responsemulti-organ carcinogenesischemical carcinogensNatural killer (NK) cell activity was evaluated after the initiation and promotion steps in a medium-term multi-organ bioassay for carcinogenesis. NK cell activity was assessed in vitro by Cr-51 release assay at the 4th and 30th weeks of the experiment. Male Wistar rats were sequentially initiated with N-diethylnitrosamine (DEN i.p.), N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN drinking water), N-methyl-N-nitrosourea (MNU i.p.), dihydroxy-di-N-propylnitrosamine (DHPN drinking water) and N,N'-dimethylhydrazine (DMH s.c.) at subcarcinogenic doses for 4 weeks (DMBDD initiation). One group was evaluated at the 4th week and the other was maintained without any further treatment until the 30th week. Two initiated groups were exposed through the diet to 2-acetylaminofluorene (2-AAF) or phenobarbital (PB), from the 6th until the 30th week, Five additional groups were studied to evaluate the effects of each initiator on NK activity. All groups submitted to initiation only, initiation plus promotion, or promotion only, developed significantly more preneoplastic lesions than the untreated control group. The main target organs for tumor development in the initiated animals n ere the liver and the colon, irrespective of treatment with 2-AAF or PB. NK cell activity was not affected bal exposure to genotoxic carcinogens after initiation, at the 4th week. Treatments only with PB or 2-AAF did not change NK cell activity, However, decreased NK cell activity was registered in the group only initiated with DMBDD and in the group given DMBDD+2-AAF. This late depression of NK cell activity at the 30th week could be related to the production of suppressing molecules by the tumor cells.UNESP, Fac Med, Dept Pathol, BR-18618000 Botucatu, SP, BrazilUNESP, Fac Med Vet, Dept Pathol, BR-18618000 Botucatu, SP, BrazilUNESP, Dept Microbiol & Immunol, BR-18618000 Botucatu, SP, BrazilUNESP, Inst Biosci, Dept Morphol, BR-18618000 Botucatu, SP, BrazilUNESP, Fac Med, Dept Pathol, BR-18618000 Botucatu, SP, BrazilUNESP, Fac Med Vet, Dept Pathol, BR-18618000 Botucatu, SP, BrazilUNESP, Dept Microbiol & Immunol, BR-18618000 Botucatu, SP, BrazilUNESP, Inst Biosci, Dept Morphol, BR-18618000 Botucatu, SP, BrazilJapanese Cancer AssocUniversidade Estadual Paulista (Unesp)Spinardi, ALTKaneno, R.Rodrigues, MAMSalvadori, DMFRocha, N. S.Barbisan, L. F.Ribeiro, L. R.Camargo, JLV de2020-12-10T18:00:08Z2020-12-10T18:00:08Z1999-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article101-107Japanese Journal Of Cancer Research. Tokyo: Japanese Cancer Assoc, v. 90, n. 1, p. 101-107, 1999.0910-5050http://hdl.handle.net/11449/195683WOS:00007845750001588458355506378090000-0002-4292-3298Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJapanese Journal Of Cancer Researchinfo:eu-repo/semantics/openAccess2024-09-03T13:14:31Zoai:repositorio.unesp.br:11449/195683Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Natural killer activity in a medium-term multi-organ bioassay for carcinogenesis |
title |
Natural killer activity in a medium-term multi-organ bioassay for carcinogenesis |
spellingShingle |
Natural killer activity in a medium-term multi-organ bioassay for carcinogenesis Spinardi, ALT NK cell activity immune response multi-organ carcinogenesis chemical carcinogens |
title_short |
Natural killer activity in a medium-term multi-organ bioassay for carcinogenesis |
title_full |
Natural killer activity in a medium-term multi-organ bioassay for carcinogenesis |
title_fullStr |
Natural killer activity in a medium-term multi-organ bioassay for carcinogenesis |
title_full_unstemmed |
Natural killer activity in a medium-term multi-organ bioassay for carcinogenesis |
title_sort |
Natural killer activity in a medium-term multi-organ bioassay for carcinogenesis |
author |
Spinardi, ALT |
author_facet |
Spinardi, ALT Kaneno, R. Rodrigues, MAM Salvadori, DMF Rocha, N. S. Barbisan, L. F. Ribeiro, L. R. Camargo, JLV de |
author_role |
author |
author2 |
Kaneno, R. Rodrigues, MAM Salvadori, DMF Rocha, N. S. Barbisan, L. F. Ribeiro, L. R. Camargo, JLV de |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Spinardi, ALT Kaneno, R. Rodrigues, MAM Salvadori, DMF Rocha, N. S. Barbisan, L. F. Ribeiro, L. R. Camargo, JLV de |
dc.subject.por.fl_str_mv |
NK cell activity immune response multi-organ carcinogenesis chemical carcinogens |
topic |
NK cell activity immune response multi-organ carcinogenesis chemical carcinogens |
description |
Natural killer (NK) cell activity was evaluated after the initiation and promotion steps in a medium-term multi-organ bioassay for carcinogenesis. NK cell activity was assessed in vitro by Cr-51 release assay at the 4th and 30th weeks of the experiment. Male Wistar rats were sequentially initiated with N-diethylnitrosamine (DEN i.p.), N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN drinking water), N-methyl-N-nitrosourea (MNU i.p.), dihydroxy-di-N-propylnitrosamine (DHPN drinking water) and N,N'-dimethylhydrazine (DMH s.c.) at subcarcinogenic doses for 4 weeks (DMBDD initiation). One group was evaluated at the 4th week and the other was maintained without any further treatment until the 30th week. Two initiated groups were exposed through the diet to 2-acetylaminofluorene (2-AAF) or phenobarbital (PB), from the 6th until the 30th week, Five additional groups were studied to evaluate the effects of each initiator on NK activity. All groups submitted to initiation only, initiation plus promotion, or promotion only, developed significantly more preneoplastic lesions than the untreated control group. The main target organs for tumor development in the initiated animals n ere the liver and the colon, irrespective of treatment with 2-AAF or PB. NK cell activity was not affected bal exposure to genotoxic carcinogens after initiation, at the 4th week. Treatments only with PB or 2-AAF did not change NK cell activity, However, decreased NK cell activity was registered in the group only initiated with DMBDD and in the group given DMBDD+2-AAF. This late depression of NK cell activity at the 30th week could be related to the production of suppressing molecules by the tumor cells. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-01-01 2020-12-10T18:00:08Z 2020-12-10T18:00:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Japanese Journal Of Cancer Research. Tokyo: Japanese Cancer Assoc, v. 90, n. 1, p. 101-107, 1999. 0910-5050 http://hdl.handle.net/11449/195683 WOS:000078457500015 8845835550637809 0000-0002-4292-3298 |
identifier_str_mv |
Japanese Journal Of Cancer Research. Tokyo: Japanese Cancer Assoc, v. 90, n. 1, p. 101-107, 1999. 0910-5050 WOS:000078457500015 8845835550637809 0000-0002-4292-3298 |
url |
http://hdl.handle.net/11449/195683 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Japanese Journal Of Cancer Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
101-107 |
dc.publisher.none.fl_str_mv |
Japanese Cancer Assoc |
publisher.none.fl_str_mv |
Japanese Cancer Assoc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021362020909056 |