Natural killer activity in a medium-term multi-organ bioassay for carcinogenesis

Detalhes bibliográficos
Autor(a) principal: Spinardi, ALT
Data de Publicação: 1999
Outros Autores: Kaneno, R., Rodrigues, MAM, Salvadori, DMF, Rocha, N. S., Barbisan, L. F., Ribeiro, L. R., Camargo, JLV de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/195683
Resumo: Natural killer (NK) cell activity was evaluated after the initiation and promotion steps in a medium-term multi-organ bioassay for carcinogenesis. NK cell activity was assessed in vitro by Cr-51 release assay at the 4th and 30th weeks of the experiment. Male Wistar rats were sequentially initiated with N-diethylnitrosamine (DEN i.p.), N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN drinking water), N-methyl-N-nitrosourea (MNU i.p.), dihydroxy-di-N-propylnitrosamine (DHPN drinking water) and N,N'-dimethylhydrazine (DMH s.c.) at subcarcinogenic doses for 4 weeks (DMBDD initiation). One group was evaluated at the 4th week and the other was maintained without any further treatment until the 30th week. Two initiated groups were exposed through the diet to 2-acetylaminofluorene (2-AAF) or phenobarbital (PB), from the 6th until the 30th week, Five additional groups were studied to evaluate the effects of each initiator on NK activity. All groups submitted to initiation only, initiation plus promotion, or promotion only, developed significantly more preneoplastic lesions than the untreated control group. The main target organs for tumor development in the initiated animals n ere the liver and the colon, irrespective of treatment with 2-AAF or PB. NK cell activity was not affected bal exposure to genotoxic carcinogens after initiation, at the 4th week. Treatments only with PB or 2-AAF did not change NK cell activity, However, decreased NK cell activity was registered in the group only initiated with DMBDD and in the group given DMBDD+2-AAF. This late depression of NK cell activity at the 30th week could be related to the production of suppressing molecules by the tumor cells.
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spelling Natural killer activity in a medium-term multi-organ bioassay for carcinogenesisNK cell activityimmune responsemulti-organ carcinogenesischemical carcinogensNatural killer (NK) cell activity was evaluated after the initiation and promotion steps in a medium-term multi-organ bioassay for carcinogenesis. NK cell activity was assessed in vitro by Cr-51 release assay at the 4th and 30th weeks of the experiment. Male Wistar rats were sequentially initiated with N-diethylnitrosamine (DEN i.p.), N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN drinking water), N-methyl-N-nitrosourea (MNU i.p.), dihydroxy-di-N-propylnitrosamine (DHPN drinking water) and N,N'-dimethylhydrazine (DMH s.c.) at subcarcinogenic doses for 4 weeks (DMBDD initiation). One group was evaluated at the 4th week and the other was maintained without any further treatment until the 30th week. Two initiated groups were exposed through the diet to 2-acetylaminofluorene (2-AAF) or phenobarbital (PB), from the 6th until the 30th week, Five additional groups were studied to evaluate the effects of each initiator on NK activity. All groups submitted to initiation only, initiation plus promotion, or promotion only, developed significantly more preneoplastic lesions than the untreated control group. The main target organs for tumor development in the initiated animals n ere the liver and the colon, irrespective of treatment with 2-AAF or PB. NK cell activity was not affected bal exposure to genotoxic carcinogens after initiation, at the 4th week. Treatments only with PB or 2-AAF did not change NK cell activity, However, decreased NK cell activity was registered in the group only initiated with DMBDD and in the group given DMBDD+2-AAF. This late depression of NK cell activity at the 30th week could be related to the production of suppressing molecules by the tumor cells.UNESP, Fac Med, Dept Pathol, BR-18618000 Botucatu, SP, BrazilUNESP, Fac Med Vet, Dept Pathol, BR-18618000 Botucatu, SP, BrazilUNESP, Dept Microbiol & Immunol, BR-18618000 Botucatu, SP, BrazilUNESP, Inst Biosci, Dept Morphol, BR-18618000 Botucatu, SP, BrazilUNESP, Fac Med, Dept Pathol, BR-18618000 Botucatu, SP, BrazilUNESP, Fac Med Vet, Dept Pathol, BR-18618000 Botucatu, SP, BrazilUNESP, Dept Microbiol & Immunol, BR-18618000 Botucatu, SP, BrazilUNESP, Inst Biosci, Dept Morphol, BR-18618000 Botucatu, SP, BrazilJapanese Cancer AssocUniversidade Estadual Paulista (Unesp)Spinardi, ALTKaneno, R.Rodrigues, MAMSalvadori, DMFRocha, N. S.Barbisan, L. F.Ribeiro, L. R.Camargo, JLV de2020-12-10T18:00:08Z2020-12-10T18:00:08Z1999-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article101-107Japanese Journal Of Cancer Research. Tokyo: Japanese Cancer Assoc, v. 90, n. 1, p. 101-107, 1999.0910-5050http://hdl.handle.net/11449/195683WOS:00007845750001588458355506378090000-0002-4292-3298Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJapanese Journal Of Cancer Researchinfo:eu-repo/semantics/openAccess2024-09-03T13:14:31Zoai:repositorio.unesp.br:11449/195683Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Natural killer activity in a medium-term multi-organ bioassay for carcinogenesis
title Natural killer activity in a medium-term multi-organ bioassay for carcinogenesis
spellingShingle Natural killer activity in a medium-term multi-organ bioassay for carcinogenesis
Spinardi, ALT
NK cell activity
immune response
multi-organ carcinogenesis
chemical carcinogens
title_short Natural killer activity in a medium-term multi-organ bioassay for carcinogenesis
title_full Natural killer activity in a medium-term multi-organ bioassay for carcinogenesis
title_fullStr Natural killer activity in a medium-term multi-organ bioassay for carcinogenesis
title_full_unstemmed Natural killer activity in a medium-term multi-organ bioassay for carcinogenesis
title_sort Natural killer activity in a medium-term multi-organ bioassay for carcinogenesis
author Spinardi, ALT
author_facet Spinardi, ALT
Kaneno, R.
Rodrigues, MAM
Salvadori, DMF
Rocha, N. S.
Barbisan, L. F.
Ribeiro, L. R.
Camargo, JLV de
author_role author
author2 Kaneno, R.
Rodrigues, MAM
Salvadori, DMF
Rocha, N. S.
Barbisan, L. F.
Ribeiro, L. R.
Camargo, JLV de
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Spinardi, ALT
Kaneno, R.
Rodrigues, MAM
Salvadori, DMF
Rocha, N. S.
Barbisan, L. F.
Ribeiro, L. R.
Camargo, JLV de
dc.subject.por.fl_str_mv NK cell activity
immune response
multi-organ carcinogenesis
chemical carcinogens
topic NK cell activity
immune response
multi-organ carcinogenesis
chemical carcinogens
description Natural killer (NK) cell activity was evaluated after the initiation and promotion steps in a medium-term multi-organ bioassay for carcinogenesis. NK cell activity was assessed in vitro by Cr-51 release assay at the 4th and 30th weeks of the experiment. Male Wistar rats were sequentially initiated with N-diethylnitrosamine (DEN i.p.), N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN drinking water), N-methyl-N-nitrosourea (MNU i.p.), dihydroxy-di-N-propylnitrosamine (DHPN drinking water) and N,N'-dimethylhydrazine (DMH s.c.) at subcarcinogenic doses for 4 weeks (DMBDD initiation). One group was evaluated at the 4th week and the other was maintained without any further treatment until the 30th week. Two initiated groups were exposed through the diet to 2-acetylaminofluorene (2-AAF) or phenobarbital (PB), from the 6th until the 30th week, Five additional groups were studied to evaluate the effects of each initiator on NK activity. All groups submitted to initiation only, initiation plus promotion, or promotion only, developed significantly more preneoplastic lesions than the untreated control group. The main target organs for tumor development in the initiated animals n ere the liver and the colon, irrespective of treatment with 2-AAF or PB. NK cell activity was not affected bal exposure to genotoxic carcinogens after initiation, at the 4th week. Treatments only with PB or 2-AAF did not change NK cell activity, However, decreased NK cell activity was registered in the group only initiated with DMBDD and in the group given DMBDD+2-AAF. This late depression of NK cell activity at the 30th week could be related to the production of suppressing molecules by the tumor cells.
publishDate 1999
dc.date.none.fl_str_mv 1999-01-01
2020-12-10T18:00:08Z
2020-12-10T18:00:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Japanese Journal Of Cancer Research. Tokyo: Japanese Cancer Assoc, v. 90, n. 1, p. 101-107, 1999.
0910-5050
http://hdl.handle.net/11449/195683
WOS:000078457500015
8845835550637809
0000-0002-4292-3298
identifier_str_mv Japanese Journal Of Cancer Research. Tokyo: Japanese Cancer Assoc, v. 90, n. 1, p. 101-107, 1999.
0910-5050
WOS:000078457500015
8845835550637809
0000-0002-4292-3298
url http://hdl.handle.net/11449/195683
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Japanese Journal Of Cancer Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 101-107
dc.publisher.none.fl_str_mv Japanese Cancer Assoc
publisher.none.fl_str_mv Japanese Cancer Assoc
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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