Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar rats

Detalhes bibliográficos
Autor(a) principal: Moreira, ELT
Data de Publicação: 2000
Outros Autores: de Camargo, JLV, Rodrigues, MAM, Barbisan, L. F., Salvadori, DMD
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/j.1349-7006.2000.tb00954.x
http://hdl.handle.net/11449/32466
Resumo: An initiation-promotion medium-term bioassay for detection of chemical carcinogens, developed in the male F344 rat, uses 0.1% N-bis(2-hydroxypropyl)nitrosamine (DHPN) among five genotoxic chemicals for the initiation of carcinogenesis in multiple organs. To establish this bioassay in the Wistar strain, the effects of two dose levels of DHPN were evaluated on the main DHPN rat target organs: lung, thyroid gland, kidneys and liver. Four groups of male and female animals were studied: Control--untreated group; Multi-organ initiated group (also referred to as DMBDD, based on the initials of the five initiators)-treated sequentially with N-diethylnitrosamine (DEN, i.p.), N-methyl-N-nitrosourea (MNU, i.p.), N-butyl-N-(4-hydroxy butyl)nitrosamine (BBN, drinking water), N, N'-dimethylhydrazine (DMH, s.c.) and DHPN (drinking water) for 4 weeks; a third group treated with 0.1% DHPN in drinking water for 2 weeks and the last group treated with 0.2% DHPN in drinking water for 4 weeks. The animals were sacrificed after 30 weeks. DHPN at 0.2% induced preneoplasia in the liver and kidneys of rats of both sexes, the number and area of the putative preneoplastic liver glutathione S-transferase-positive hepatocyte foci being significantly increased in these animals. It also induced benign and malignant tumors in female and in male rats. However, there was no relationship between the increased incidence of preneoplastic lesions and tumor development in the 0.2% DHPN-exposed groups of both sexes. DHPN at 0.1% induced only a few preneoplastic lesions in the liver and kidney and no tumors in both male and female rats. A clear dose and sex-related carcinogenic activity of DHPN was registered, although Wistar rats of both sexes showed a relative resistance to the carcinogenic activity of this compound.
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spelling Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar ratsN-bis(2-hydroxypropyl)nitrosamine (DHPN)Wistar ratmulti-organ carcinogenesispreneoplasia and neoplasiachemical carcinogensAn initiation-promotion medium-term bioassay for detection of chemical carcinogens, developed in the male F344 rat, uses 0.1% N-bis(2-hydroxypropyl)nitrosamine (DHPN) among five genotoxic chemicals for the initiation of carcinogenesis in multiple organs. To establish this bioassay in the Wistar strain, the effects of two dose levels of DHPN were evaluated on the main DHPN rat target organs: lung, thyroid gland, kidneys and liver. Four groups of male and female animals were studied: Control--untreated group; Multi-organ initiated group (also referred to as DMBDD, based on the initials of the five initiators)-treated sequentially with N-diethylnitrosamine (DEN, i.p.), N-methyl-N-nitrosourea (MNU, i.p.), N-butyl-N-(4-hydroxy butyl)nitrosamine (BBN, drinking water), N, N'-dimethylhydrazine (DMH, s.c.) and DHPN (drinking water) for 4 weeks; a third group treated with 0.1% DHPN in drinking water for 2 weeks and the last group treated with 0.2% DHPN in drinking water for 4 weeks. The animals were sacrificed after 30 weeks. DHPN at 0.2% induced preneoplasia in the liver and kidneys of rats of both sexes, the number and area of the putative preneoplastic liver glutathione S-transferase-positive hepatocyte foci being significantly increased in these animals. It also induced benign and malignant tumors in female and in male rats. However, there was no relationship between the increased incidence of preneoplastic lesions and tumor development in the 0.2% DHPN-exposed groups of both sexes. DHPN at 0.1% induced only a few preneoplastic lesions in the liver and kidney and no tumors in both male and female rats. A clear dose and sex-related carcinogenic activity of DHPN was registered, although Wistar rats of both sexes showed a relative resistance to the carcinogenic activity of this compound.UNESP, Fac Med, Dept Patol, BR-18618000 São Paulo, BrazilUFBA, Escola Med Vet, Dept Patol & Clin, BR-40170110 Buenos Aires, DF, BrazilInst Biociencias, Dept Morfol, BR-18618000 Botucatu, SP, BrazilUNESP, Fac Med, Dept Patol, BR-18618000 São Paulo, BrazilBusiness Center Academic Societies JapanUniversidade Estadual Paulista (Unesp)Universidade Federal da Bahia (UFBA)Universidade de São Paulo (USP)Moreira, ELTde Camargo, JLVRodrigues, MAMBarbisan, L. F.Salvadori, DMD2014-05-20T15:21:18Z2014-05-20T15:21:18Z2000-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article368-374application/pdfhttp://dx.doi.org/10.1111/j.1349-7006.2000.tb00954.xJapanese Journal of Cancer Research. Tokyo: Business Center Academic Societies Japan, v. 91, n. 4, p. 368-374, 2000.0910-5050http://hdl.handle.net/11449/3246610.1111/j.1349-7006.2000.tb00954.xWOS:000086703100002WOS000086703100002.pdf3278528112652257Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJapanese Journal of Cancer Researchinfo:eu-repo/semantics/openAccess2024-01-11T06:31:56Zoai:repositorio.unesp.br:11449/32466Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:43:42.633969Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar rats
title Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar rats
spellingShingle Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar rats
Moreira, ELT
N-bis(2-hydroxypropyl)nitrosamine (DHPN)
Wistar rat
multi-organ carcinogenesis
preneoplasia and neoplasia
chemical carcinogens
title_short Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar rats
title_full Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar rats
title_fullStr Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar rats
title_full_unstemmed Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar rats
title_sort Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar rats
author Moreira, ELT
author_facet Moreira, ELT
de Camargo, JLV
Rodrigues, MAM
Barbisan, L. F.
Salvadori, DMD
author_role author
author2 de Camargo, JLV
Rodrigues, MAM
Barbisan, L. F.
Salvadori, DMD
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal da Bahia (UFBA)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Moreira, ELT
de Camargo, JLV
Rodrigues, MAM
Barbisan, L. F.
Salvadori, DMD
dc.subject.por.fl_str_mv N-bis(2-hydroxypropyl)nitrosamine (DHPN)
Wistar rat
multi-organ carcinogenesis
preneoplasia and neoplasia
chemical carcinogens
topic N-bis(2-hydroxypropyl)nitrosamine (DHPN)
Wistar rat
multi-organ carcinogenesis
preneoplasia and neoplasia
chemical carcinogens
description An initiation-promotion medium-term bioassay for detection of chemical carcinogens, developed in the male F344 rat, uses 0.1% N-bis(2-hydroxypropyl)nitrosamine (DHPN) among five genotoxic chemicals for the initiation of carcinogenesis in multiple organs. To establish this bioassay in the Wistar strain, the effects of two dose levels of DHPN were evaluated on the main DHPN rat target organs: lung, thyroid gland, kidneys and liver. Four groups of male and female animals were studied: Control--untreated group; Multi-organ initiated group (also referred to as DMBDD, based on the initials of the five initiators)-treated sequentially with N-diethylnitrosamine (DEN, i.p.), N-methyl-N-nitrosourea (MNU, i.p.), N-butyl-N-(4-hydroxy butyl)nitrosamine (BBN, drinking water), N, N'-dimethylhydrazine (DMH, s.c.) and DHPN (drinking water) for 4 weeks; a third group treated with 0.1% DHPN in drinking water for 2 weeks and the last group treated with 0.2% DHPN in drinking water for 4 weeks. The animals were sacrificed after 30 weeks. DHPN at 0.2% induced preneoplasia in the liver and kidneys of rats of both sexes, the number and area of the putative preneoplastic liver glutathione S-transferase-positive hepatocyte foci being significantly increased in these animals. It also induced benign and malignant tumors in female and in male rats. However, there was no relationship between the increased incidence of preneoplastic lesions and tumor development in the 0.2% DHPN-exposed groups of both sexes. DHPN at 0.1% induced only a few preneoplastic lesions in the liver and kidney and no tumors in both male and female rats. A clear dose and sex-related carcinogenic activity of DHPN was registered, although Wistar rats of both sexes showed a relative resistance to the carcinogenic activity of this compound.
publishDate 2000
dc.date.none.fl_str_mv 2000-04-01
2014-05-20T15:21:18Z
2014-05-20T15:21:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/j.1349-7006.2000.tb00954.x
Japanese Journal of Cancer Research. Tokyo: Business Center Academic Societies Japan, v. 91, n. 4, p. 368-374, 2000.
0910-5050
http://hdl.handle.net/11449/32466
10.1111/j.1349-7006.2000.tb00954.x
WOS:000086703100002
WOS000086703100002.pdf
3278528112652257
url http://dx.doi.org/10.1111/j.1349-7006.2000.tb00954.x
http://hdl.handle.net/11449/32466
identifier_str_mv Japanese Journal of Cancer Research. Tokyo: Business Center Academic Societies Japan, v. 91, n. 4, p. 368-374, 2000.
0910-5050
10.1111/j.1349-7006.2000.tb00954.x
WOS:000086703100002
WOS000086703100002.pdf
3278528112652257
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Japanese Journal of Cancer Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 368-374
application/pdf
dc.publisher.none.fl_str_mv Business Center Academic Societies Japan
publisher.none.fl_str_mv Business Center Academic Societies Japan
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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