Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2000 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/j.1349-7006.2000.tb00954.x http://hdl.handle.net/11449/32466 |
Resumo: | An initiation-promotion medium-term bioassay for detection of chemical carcinogens, developed in the male F344 rat, uses 0.1% N-bis(2-hydroxypropyl)nitrosamine (DHPN) among five genotoxic chemicals for the initiation of carcinogenesis in multiple organs. To establish this bioassay in the Wistar strain, the effects of two dose levels of DHPN were evaluated on the main DHPN rat target organs: lung, thyroid gland, kidneys and liver. Four groups of male and female animals were studied: Control--untreated group; Multi-organ initiated group (also referred to as DMBDD, based on the initials of the five initiators)-treated sequentially with N-diethylnitrosamine (DEN, i.p.), N-methyl-N-nitrosourea (MNU, i.p.), N-butyl-N-(4-hydroxy butyl)nitrosamine (BBN, drinking water), N, N'-dimethylhydrazine (DMH, s.c.) and DHPN (drinking water) for 4 weeks; a third group treated with 0.1% DHPN in drinking water for 2 weeks and the last group treated with 0.2% DHPN in drinking water for 4 weeks. The animals were sacrificed after 30 weeks. DHPN at 0.2% induced preneoplasia in the liver and kidneys of rats of both sexes, the number and area of the putative preneoplastic liver glutathione S-transferase-positive hepatocyte foci being significantly increased in these animals. It also induced benign and malignant tumors in female and in male rats. However, there was no relationship between the increased incidence of preneoplastic lesions and tumor development in the 0.2% DHPN-exposed groups of both sexes. DHPN at 0.1% induced only a few preneoplastic lesions in the liver and kidney and no tumors in both male and female rats. A clear dose and sex-related carcinogenic activity of DHPN was registered, although Wistar rats of both sexes showed a relative resistance to the carcinogenic activity of this compound. |
id |
UNSP_f668ed1d9370f3499ef8ba93eac3a722 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/32466 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar ratsN-bis(2-hydroxypropyl)nitrosamine (DHPN)Wistar ratmulti-organ carcinogenesispreneoplasia and neoplasiachemical carcinogensAn initiation-promotion medium-term bioassay for detection of chemical carcinogens, developed in the male F344 rat, uses 0.1% N-bis(2-hydroxypropyl)nitrosamine (DHPN) among five genotoxic chemicals for the initiation of carcinogenesis in multiple organs. To establish this bioassay in the Wistar strain, the effects of two dose levels of DHPN were evaluated on the main DHPN rat target organs: lung, thyroid gland, kidneys and liver. Four groups of male and female animals were studied: Control--untreated group; Multi-organ initiated group (also referred to as DMBDD, based on the initials of the five initiators)-treated sequentially with N-diethylnitrosamine (DEN, i.p.), N-methyl-N-nitrosourea (MNU, i.p.), N-butyl-N-(4-hydroxy butyl)nitrosamine (BBN, drinking water), N, N'-dimethylhydrazine (DMH, s.c.) and DHPN (drinking water) for 4 weeks; a third group treated with 0.1% DHPN in drinking water for 2 weeks and the last group treated with 0.2% DHPN in drinking water for 4 weeks. The animals were sacrificed after 30 weeks. DHPN at 0.2% induced preneoplasia in the liver and kidneys of rats of both sexes, the number and area of the putative preneoplastic liver glutathione S-transferase-positive hepatocyte foci being significantly increased in these animals. It also induced benign and malignant tumors in female and in male rats. However, there was no relationship between the increased incidence of preneoplastic lesions and tumor development in the 0.2% DHPN-exposed groups of both sexes. DHPN at 0.1% induced only a few preneoplastic lesions in the liver and kidney and no tumors in both male and female rats. A clear dose and sex-related carcinogenic activity of DHPN was registered, although Wistar rats of both sexes showed a relative resistance to the carcinogenic activity of this compound.UNESP, Fac Med, Dept Patol, BR-18618000 São Paulo, BrazilUFBA, Escola Med Vet, Dept Patol & Clin, BR-40170110 Buenos Aires, DF, BrazilInst Biociencias, Dept Morfol, BR-18618000 Botucatu, SP, BrazilUNESP, Fac Med, Dept Patol, BR-18618000 São Paulo, BrazilBusiness Center Academic Societies JapanUniversidade Estadual Paulista (Unesp)Universidade Federal da Bahia (UFBA)Universidade de São Paulo (USP)Moreira, ELTde Camargo, JLVRodrigues, MAMBarbisan, L. F.Salvadori, DMD2014-05-20T15:21:18Z2014-05-20T15:21:18Z2000-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article368-374application/pdfhttp://dx.doi.org/10.1111/j.1349-7006.2000.tb00954.xJapanese Journal of Cancer Research. Tokyo: Business Center Academic Societies Japan, v. 91, n. 4, p. 368-374, 2000.0910-5050http://hdl.handle.net/11449/3246610.1111/j.1349-7006.2000.tb00954.xWOS:000086703100002WOS000086703100002.pdf3278528112652257Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJapanese Journal of Cancer Researchinfo:eu-repo/semantics/openAccess2024-01-11T06:31:56Zoai:repositorio.unesp.br:11449/32466Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:43:42.633969Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar rats |
title |
Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar rats |
spellingShingle |
Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar rats Moreira, ELT N-bis(2-hydroxypropyl)nitrosamine (DHPN) Wistar rat multi-organ carcinogenesis preneoplasia and neoplasia chemical carcinogens |
title_short |
Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar rats |
title_full |
Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar rats |
title_fullStr |
Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar rats |
title_full_unstemmed |
Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar rats |
title_sort |
Dose- and sex-related carcinogenesis by N-bis(2-hydroxypropyl)nitrosamine in Wistar rats |
author |
Moreira, ELT |
author_facet |
Moreira, ELT de Camargo, JLV Rodrigues, MAM Barbisan, L. F. Salvadori, DMD |
author_role |
author |
author2 |
de Camargo, JLV Rodrigues, MAM Barbisan, L. F. Salvadori, DMD |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal da Bahia (UFBA) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Moreira, ELT de Camargo, JLV Rodrigues, MAM Barbisan, L. F. Salvadori, DMD |
dc.subject.por.fl_str_mv |
N-bis(2-hydroxypropyl)nitrosamine (DHPN) Wistar rat multi-organ carcinogenesis preneoplasia and neoplasia chemical carcinogens |
topic |
N-bis(2-hydroxypropyl)nitrosamine (DHPN) Wistar rat multi-organ carcinogenesis preneoplasia and neoplasia chemical carcinogens |
description |
An initiation-promotion medium-term bioassay for detection of chemical carcinogens, developed in the male F344 rat, uses 0.1% N-bis(2-hydroxypropyl)nitrosamine (DHPN) among five genotoxic chemicals for the initiation of carcinogenesis in multiple organs. To establish this bioassay in the Wistar strain, the effects of two dose levels of DHPN were evaluated on the main DHPN rat target organs: lung, thyroid gland, kidneys and liver. Four groups of male and female animals were studied: Control--untreated group; Multi-organ initiated group (also referred to as DMBDD, based on the initials of the five initiators)-treated sequentially with N-diethylnitrosamine (DEN, i.p.), N-methyl-N-nitrosourea (MNU, i.p.), N-butyl-N-(4-hydroxy butyl)nitrosamine (BBN, drinking water), N, N'-dimethylhydrazine (DMH, s.c.) and DHPN (drinking water) for 4 weeks; a third group treated with 0.1% DHPN in drinking water for 2 weeks and the last group treated with 0.2% DHPN in drinking water for 4 weeks. The animals were sacrificed after 30 weeks. DHPN at 0.2% induced preneoplasia in the liver and kidneys of rats of both sexes, the number and area of the putative preneoplastic liver glutathione S-transferase-positive hepatocyte foci being significantly increased in these animals. It also induced benign and malignant tumors in female and in male rats. However, there was no relationship between the increased incidence of preneoplastic lesions and tumor development in the 0.2% DHPN-exposed groups of both sexes. DHPN at 0.1% induced only a few preneoplastic lesions in the liver and kidney and no tumors in both male and female rats. A clear dose and sex-related carcinogenic activity of DHPN was registered, although Wistar rats of both sexes showed a relative resistance to the carcinogenic activity of this compound. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-04-01 2014-05-20T15:21:18Z 2014-05-20T15:21:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/j.1349-7006.2000.tb00954.x Japanese Journal of Cancer Research. Tokyo: Business Center Academic Societies Japan, v. 91, n. 4, p. 368-374, 2000. 0910-5050 http://hdl.handle.net/11449/32466 10.1111/j.1349-7006.2000.tb00954.x WOS:000086703100002 WOS000086703100002.pdf 3278528112652257 |
url |
http://dx.doi.org/10.1111/j.1349-7006.2000.tb00954.x http://hdl.handle.net/11449/32466 |
identifier_str_mv |
Japanese Journal of Cancer Research. Tokyo: Business Center Academic Societies Japan, v. 91, n. 4, p. 368-374, 2000. 0910-5050 10.1111/j.1349-7006.2000.tb00954.x WOS:000086703100002 WOS000086703100002.pdf 3278528112652257 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Japanese Journal of Cancer Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
368-374 application/pdf |
dc.publisher.none.fl_str_mv |
Business Center Academic Societies Japan |
publisher.none.fl_str_mv |
Business Center Academic Societies Japan |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129454842576896 |