Vibrational Spectroscopic Characterization and Coherent Anti-Stokes Raman Spectroscopy (CARS) Imaging of Artepillin C

• Autor(a) principal: Pazin, Wallance M. [UNESP]
• Data de Publicação: 2020
• Outros Autores: Furini, Leonardo N. [UNESP], Solovyeva, Vita, Lemma, Tibebe [UNESP], Rubira, Rafael J. G. [UNESP], Jorgensen, Bjarke, Constantino, Carlos J. L. [UNESP], Brewer, Jonathan R.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1177/0003702820904456; http://hdl.handle.net/11449/196894
• topic: Artepillin C; propolis; Raman spectroscopy; CARS microscopy; surface-enhanced Raman scattering; SERS; imaging techniques

In the following work, the vibrational spectroscopic characteristics of artepillin C are reported by means of Fourier transform infrared (FT-IR) and Raman spectroscopies, surface-enhanced Raman scattering (SERS), and coherent anti-Stokes Raman scattering (CARS) microscopy. Artepillin C is an interesting compound due to its pharmacological properties, including antitumor activity. It is found as the major component of Brazilian green propolis, a resinous mixture produced by bees to protect their hives against intruders. Vibrational spectroscopic techniques have shown a strong peak at 1599 cm(-1), assigned to C=C stretching vibrations from the aromatic ring of artepillin C. From these data, direct visualization of artepillin C could be assessed by means of CARS microscopy, showing differences in the film hydration obtained for its neutral and deprotonated states. Raman-based methods show potential to visualize the uptake and action of artepillin C in biological systems, triggering its interaction with biological systems that are needed to understand its mechanism of action.