Comparison between in vitro embryo production using Y-sorted sperm and timed artificial insemination with non-sorted sperm to produce crossbred calves
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.anireprosci.2019.06.013 http://hdl.handle.net/11449/190442 |
Resumo: | Due to the increasing use of in vitro embryo production (IVEP) and the importance of crossbreeding for beef production, pregnancy rates of Nelore recipients were evaluated following Fixed Time Embryo Transfer with fresh or vitrified IVEP embryos produced with Y-sorted sperm of Angus bulls (B. taurus) or Fixed Time Artificial Insemination using non-sorted sperm. For IVEP in Experiment 1, oocytes were obtained using Ovum Pick Up (OPU) (n = 84 embryos) or from ovaries from a slaughterhouse (SLAUGHTER, n = 66 embryos). In Experiment 2, with oocytes obtained by OPU, IVEP embryos were fresh (FRESH, n = 271) or after vitrification/warming (VITRIFIED, n = 79) and PR was compared with FTAI (n = 239). In Experiment 1, cleavage rates were 63.8% and 39.1% for OPU and SLAUGHTER groups, respectively (P = 0.02), and blastocyst rates were 30.5% and 14.7%, respectively (P = 0.09). The PR was similar when considering the source of oocytes (OPU = 35.7%; SLAUGHTER = 25.8%; P = 0.17). In Experiment 2, there was no difference in PR for FRESH or VITRIFIED embryos (34.3% and 30.4%, respectively, P = 0.72), but lesser than FTAI (47.7, P = 0.002). It is concluded that the IVEP with Y-sorted sperm associated with vitrification or embryos produced with oocytes from different sources did not affect PR when there was transfer of crossbred embryos into recipients, and can optimize large-scale application of IVEP technology; however, FTAI pregnancy rates with non-sex sorted sperm were greater. |
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Repositório Institucional da UNESP |
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Comparison between in vitro embryo production using Y-sorted sperm and timed artificial insemination with non-sorted sperm to produce crossbred calvesCrossbreedingCryopreservationFTAIFTETNelore-AngusDue to the increasing use of in vitro embryo production (IVEP) and the importance of crossbreeding for beef production, pregnancy rates of Nelore recipients were evaluated following Fixed Time Embryo Transfer with fresh or vitrified IVEP embryos produced with Y-sorted sperm of Angus bulls (B. taurus) or Fixed Time Artificial Insemination using non-sorted sperm. For IVEP in Experiment 1, oocytes were obtained using Ovum Pick Up (OPU) (n = 84 embryos) or from ovaries from a slaughterhouse (SLAUGHTER, n = 66 embryos). In Experiment 2, with oocytes obtained by OPU, IVEP embryos were fresh (FRESH, n = 271) or after vitrification/warming (VITRIFIED, n = 79) and PR was compared with FTAI (n = 239). In Experiment 1, cleavage rates were 63.8% and 39.1% for OPU and SLAUGHTER groups, respectively (P = 0.02), and blastocyst rates were 30.5% and 14.7%, respectively (P = 0.09). The PR was similar when considering the source of oocytes (OPU = 35.7%; SLAUGHTER = 25.8%; P = 0.17). In Experiment 2, there was no difference in PR for FRESH or VITRIFIED embryos (34.3% and 30.4%, respectively, P = 0.72), but lesser than FTAI (47.7, P = 0.002). It is concluded that the IVEP with Y-sorted sperm associated with vitrification or embryos produced with oocytes from different sources did not affect PR when there was transfer of crossbred embryos into recipients, and can optimize large-scale application of IVEP technology; however, FTAI pregnancy rates with non-sex sorted sperm were greater.Empresa Brasileira de Pesquisa AgropecuáriaPrograma de Pós-Graduação em Ciências Veterinárias UFMS, Av. Senador Felinto Muller, 2443Embrapa Gado de Corte, Av. Rádio Maia, 830, Vila PopularUNESP Araçatuba FMV, Rua Clovis Pestana, 793Embrapa Pantanal, Rua 21 de Setembro, 1880UNESP Araçatuba FMV, Rua Clovis Pestana, 793Empresa Brasileira de Pesquisa Agropecuária: 02.13.14.011.00.00Universidade Federal de Mato Grosso do Sul (UFMS)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)de Oliveira Bezerra, AlexandreNicacio, Alessandra Corallode Oliveira Menezes, Gilberto Romeiroda Costa Gomes, Rodrigoda Silva, Luiz Otavio Camposde Souza Rocha-Frigoni, Nathália Alves [UNESP]Mingoti, Gisele Zoccal [UNESP]da Silva Leão, Beatriz Caetano [UNESP]da Costa e Silva, Eliane ViannaNogueira, Ériklis2019-10-06T17:13:23Z2019-10-06T17:13:23Z2019-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.anireprosci.2019.06.013Animal Reproduction Science, v. 208.0378-4320http://hdl.handle.net/11449/19044210.1016/j.anireprosci.2019.06.0132-s2.0-85068075121Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Reproduction Scienceinfo:eu-repo/semantics/openAccess2021-10-23T17:30:23Zoai:repositorio.unesp.br:11449/190442Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T17:30:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparison between in vitro embryo production using Y-sorted sperm and timed artificial insemination with non-sorted sperm to produce crossbred calves |
title |
Comparison between in vitro embryo production using Y-sorted sperm and timed artificial insemination with non-sorted sperm to produce crossbred calves |
spellingShingle |
Comparison between in vitro embryo production using Y-sorted sperm and timed artificial insemination with non-sorted sperm to produce crossbred calves de Oliveira Bezerra, Alexandre Crossbreeding Cryopreservation FTAI FTET Nelore-Angus |
title_short |
Comparison between in vitro embryo production using Y-sorted sperm and timed artificial insemination with non-sorted sperm to produce crossbred calves |
title_full |
Comparison between in vitro embryo production using Y-sorted sperm and timed artificial insemination with non-sorted sperm to produce crossbred calves |
title_fullStr |
Comparison between in vitro embryo production using Y-sorted sperm and timed artificial insemination with non-sorted sperm to produce crossbred calves |
title_full_unstemmed |
Comparison between in vitro embryo production using Y-sorted sperm and timed artificial insemination with non-sorted sperm to produce crossbred calves |
title_sort |
Comparison between in vitro embryo production using Y-sorted sperm and timed artificial insemination with non-sorted sperm to produce crossbred calves |
author |
de Oliveira Bezerra, Alexandre |
author_facet |
de Oliveira Bezerra, Alexandre Nicacio, Alessandra Corallo de Oliveira Menezes, Gilberto Romeiro da Costa Gomes, Rodrigo da Silva, Luiz Otavio Campos de Souza Rocha-Frigoni, Nathália Alves [UNESP] Mingoti, Gisele Zoccal [UNESP] da Silva Leão, Beatriz Caetano [UNESP] da Costa e Silva, Eliane Vianna Nogueira, Ériklis |
author_role |
author |
author2 |
Nicacio, Alessandra Corallo de Oliveira Menezes, Gilberto Romeiro da Costa Gomes, Rodrigo da Silva, Luiz Otavio Campos de Souza Rocha-Frigoni, Nathália Alves [UNESP] Mingoti, Gisele Zoccal [UNESP] da Silva Leão, Beatriz Caetano [UNESP] da Costa e Silva, Eliane Vianna Nogueira, Ériklis |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Mato Grosso do Sul (UFMS) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
de Oliveira Bezerra, Alexandre Nicacio, Alessandra Corallo de Oliveira Menezes, Gilberto Romeiro da Costa Gomes, Rodrigo da Silva, Luiz Otavio Campos de Souza Rocha-Frigoni, Nathália Alves [UNESP] Mingoti, Gisele Zoccal [UNESP] da Silva Leão, Beatriz Caetano [UNESP] da Costa e Silva, Eliane Vianna Nogueira, Ériklis |
dc.subject.por.fl_str_mv |
Crossbreeding Cryopreservation FTAI FTET Nelore-Angus |
topic |
Crossbreeding Cryopreservation FTAI FTET Nelore-Angus |
description |
Due to the increasing use of in vitro embryo production (IVEP) and the importance of crossbreeding for beef production, pregnancy rates of Nelore recipients were evaluated following Fixed Time Embryo Transfer with fresh or vitrified IVEP embryos produced with Y-sorted sperm of Angus bulls (B. taurus) or Fixed Time Artificial Insemination using non-sorted sperm. For IVEP in Experiment 1, oocytes were obtained using Ovum Pick Up (OPU) (n = 84 embryos) or from ovaries from a slaughterhouse (SLAUGHTER, n = 66 embryos). In Experiment 2, with oocytes obtained by OPU, IVEP embryos were fresh (FRESH, n = 271) or after vitrification/warming (VITRIFIED, n = 79) and PR was compared with FTAI (n = 239). In Experiment 1, cleavage rates were 63.8% and 39.1% for OPU and SLAUGHTER groups, respectively (P = 0.02), and blastocyst rates were 30.5% and 14.7%, respectively (P = 0.09). The PR was similar when considering the source of oocytes (OPU = 35.7%; SLAUGHTER = 25.8%; P = 0.17). In Experiment 2, there was no difference in PR for FRESH or VITRIFIED embryos (34.3% and 30.4%, respectively, P = 0.72), but lesser than FTAI (47.7, P = 0.002). It is concluded that the IVEP with Y-sorted sperm associated with vitrification or embryos produced with oocytes from different sources did not affect PR when there was transfer of crossbred embryos into recipients, and can optimize large-scale application of IVEP technology; however, FTAI pregnancy rates with non-sex sorted sperm were greater. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T17:13:23Z 2019-10-06T17:13:23Z 2019-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.anireprosci.2019.06.013 Animal Reproduction Science, v. 208. 0378-4320 http://hdl.handle.net/11449/190442 10.1016/j.anireprosci.2019.06.013 2-s2.0-85068075121 |
url |
http://dx.doi.org/10.1016/j.anireprosci.2019.06.013 http://hdl.handle.net/11449/190442 |
identifier_str_mv |
Animal Reproduction Science, v. 208. 0378-4320 10.1016/j.anireprosci.2019.06.013 2-s2.0-85068075121 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Reproduction Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803649277738614784 |