Proteomic characterization of the fibroin-based silk fibers produced by weaver ant Camponotus textor
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jprot.2022.104579 http://hdl.handle.net/11449/240857 |
Resumo: | The fibroin-based silk fibers of weaver ants are an alternative biomaterial to be investigated and explored for potential biomedical applications. In this context, the silk fibers from the nest of the weaver ant Camponotus textor was solubilized and fractionated by gel permeation. The different fractions were collected, pooled and submitted to analysis with a series of biochemical methods, nuclear magnetic resonance (NMR) spectroscopy, analytical proteomic strategies, and data treatment with bioinformatic tools to perform the structural characterization of the fibroin-based silk fibers produced by the ant. Our data demonstrated the identification of one fibroin proteoform in the ant silk fibers. The protein chracterized as a glycoprotein with MW around 40 kDa and presenting 66% (w/w) of total sugars attached to it through O-linked carbohydrates. The 3D of protein was modeled, revealing a structure predominantly constituted of coiled-coil secondary units in the whole model, featuring at least four superhelices (arrangement with multiple α-helices). The scientific outcomes reported herein may be relevant for the development of novel approaches for the synthetic or recombinant production of novel silk-based polymers for biomedical applications. Biological significance: The present investigation significantly expanded knowledge regarding to the fibroin-based silk fibers from weaver ants, contributing to improvements in our understanding of the properties and characteristics of these silk fibers. For example, as reported here, carbohydrates were detected in the ants' silk for the first time presenting the fibroin as a glycoprotein. Moreover, the 3D structure provided new insights into the secondary structures considering the whole model of the protein. |
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Proteomic characterization of the fibroin-based silk fibers produced by weaver ant Camponotus textorAnt silk proteinsCarbohydratesFibroinsLCMS-based proteomicsNMRThe fibroin-based silk fibers of weaver ants are an alternative biomaterial to be investigated and explored for potential biomedical applications. In this context, the silk fibers from the nest of the weaver ant Camponotus textor was solubilized and fractionated by gel permeation. The different fractions were collected, pooled and submitted to analysis with a series of biochemical methods, nuclear magnetic resonance (NMR) spectroscopy, analytical proteomic strategies, and data treatment with bioinformatic tools to perform the structural characterization of the fibroin-based silk fibers produced by the ant. Our data demonstrated the identification of one fibroin proteoform in the ant silk fibers. The protein chracterized as a glycoprotein with MW around 40 kDa and presenting 66% (w/w) of total sugars attached to it through O-linked carbohydrates. The 3D of protein was modeled, revealing a structure predominantly constituted of coiled-coil secondary units in the whole model, featuring at least four superhelices (arrangement with multiple α-helices). The scientific outcomes reported herein may be relevant for the development of novel approaches for the synthetic or recombinant production of novel silk-based polymers for biomedical applications. Biological significance: The present investigation significantly expanded knowledge regarding to the fibroin-based silk fibers from weaver ants, contributing to improvements in our understanding of the properties and characteristics of these silk fibers. For example, as reported here, carbohydrates were detected in the ants' silk for the first time presenting the fibroin as a glycoprotein. Moreover, the 3D structure provided new insights into the secondary structures considering the whole model of the protein.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of General and Applied Biology Institute of Biosciences of Rio Claro University of Sao Paulo State (UNESP), Rio ClaroInstitute of Chemistry University of Campinas (UNICAMP), CampinasBiomedical Research Division Center for Genetic Engineering and Biotechnology (CIGB)Department of General and Applied Biology Institute of Biosciences of Rio Claro University of Sao Paulo State (UNESP), Rio ClaroUniversidade Estadual Paulista (UNESP)Universidade Estadual de Campinas (UNICAMP)Center for Genetic Engineering and Biotechnology (CIGB)Pinto, José Roberto Aparecido dos Santos [UNESP]Esteves, Franciele Grego [UNESP]Tormena, Cláudio FranciscoPerez-Riverol, Amilcar [UNESP]Lasa, Alexis MusacchioBueno, Odair Correa [UNESP]Palma, Mario Sergio [UNESP]2023-03-01T20:35:51Z2023-03-01T20:35:51Z2022-06-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jprot.2022.104579Journal of Proteomics, v. 261.1876-77371874-3919http://hdl.handle.net/11449/24085710.1016/j.jprot.2022.1045792-s2.0-85128453457Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Proteomicsinfo:eu-repo/semantics/openAccess2023-03-01T20:35:52Zoai:repositorio.unesp.br:11449/240857Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-05-23T20:00:18.441570Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Proteomic characterization of the fibroin-based silk fibers produced by weaver ant Camponotus textor |
title |
Proteomic characterization of the fibroin-based silk fibers produced by weaver ant Camponotus textor |
spellingShingle |
Proteomic characterization of the fibroin-based silk fibers produced by weaver ant Camponotus textor Pinto, José Roberto Aparecido dos Santos [UNESP] Ant silk proteins Carbohydrates Fibroins LCMS-based proteomics NMR |
title_short |
Proteomic characterization of the fibroin-based silk fibers produced by weaver ant Camponotus textor |
title_full |
Proteomic characterization of the fibroin-based silk fibers produced by weaver ant Camponotus textor |
title_fullStr |
Proteomic characterization of the fibroin-based silk fibers produced by weaver ant Camponotus textor |
title_full_unstemmed |
Proteomic characterization of the fibroin-based silk fibers produced by weaver ant Camponotus textor |
title_sort |
Proteomic characterization of the fibroin-based silk fibers produced by weaver ant Camponotus textor |
author |
Pinto, José Roberto Aparecido dos Santos [UNESP] |
author_facet |
Pinto, José Roberto Aparecido dos Santos [UNESP] Esteves, Franciele Grego [UNESP] Tormena, Cláudio Francisco Perez-Riverol, Amilcar [UNESP] Lasa, Alexis Musacchio Bueno, Odair Correa [UNESP] Palma, Mario Sergio [UNESP] |
author_role |
author |
author2 |
Esteves, Franciele Grego [UNESP] Tormena, Cláudio Francisco Perez-Riverol, Amilcar [UNESP] Lasa, Alexis Musacchio Bueno, Odair Correa [UNESP] Palma, Mario Sergio [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Estadual de Campinas (UNICAMP) Center for Genetic Engineering and Biotechnology (CIGB) |
dc.contributor.author.fl_str_mv |
Pinto, José Roberto Aparecido dos Santos [UNESP] Esteves, Franciele Grego [UNESP] Tormena, Cláudio Francisco Perez-Riverol, Amilcar [UNESP] Lasa, Alexis Musacchio Bueno, Odair Correa [UNESP] Palma, Mario Sergio [UNESP] |
dc.subject.por.fl_str_mv |
Ant silk proteins Carbohydrates Fibroins LCMS-based proteomics NMR |
topic |
Ant silk proteins Carbohydrates Fibroins LCMS-based proteomics NMR |
description |
The fibroin-based silk fibers of weaver ants are an alternative biomaterial to be investigated and explored for potential biomedical applications. In this context, the silk fibers from the nest of the weaver ant Camponotus textor was solubilized and fractionated by gel permeation. The different fractions were collected, pooled and submitted to analysis with a series of biochemical methods, nuclear magnetic resonance (NMR) spectroscopy, analytical proteomic strategies, and data treatment with bioinformatic tools to perform the structural characterization of the fibroin-based silk fibers produced by the ant. Our data demonstrated the identification of one fibroin proteoform in the ant silk fibers. The protein chracterized as a glycoprotein with MW around 40 kDa and presenting 66% (w/w) of total sugars attached to it through O-linked carbohydrates. The 3D of protein was modeled, revealing a structure predominantly constituted of coiled-coil secondary units in the whole model, featuring at least four superhelices (arrangement with multiple α-helices). The scientific outcomes reported herein may be relevant for the development of novel approaches for the synthetic or recombinant production of novel silk-based polymers for biomedical applications. Biological significance: The present investigation significantly expanded knowledge regarding to the fibroin-based silk fibers from weaver ants, contributing to improvements in our understanding of the properties and characteristics of these silk fibers. For example, as reported here, carbohydrates were detected in the ants' silk for the first time presenting the fibroin as a glycoprotein. Moreover, the 3D structure provided new insights into the secondary structures considering the whole model of the protein. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-06-15 2023-03-01T20:35:51Z 2023-03-01T20:35:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jprot.2022.104579 Journal of Proteomics, v. 261. 1876-7737 1874-3919 http://hdl.handle.net/11449/240857 10.1016/j.jprot.2022.104579 2-s2.0-85128453457 |
url |
http://dx.doi.org/10.1016/j.jprot.2022.104579 http://hdl.handle.net/11449/240857 |
identifier_str_mv |
Journal of Proteomics, v. 261. 1876-7737 1874-3919 10.1016/j.jprot.2022.104579 2-s2.0-85128453457 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Proteomics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803045643706433536 |