Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1415-47572007000600010 http://hdl.handle.net/11449/30502 |
Resumo: | An alpha actin gene segment, isolated from Nile tilapia (Oreochromis niloticus), was characterized by nucleotide sequencing, predicted amino acid sequence and Southern blot hybridization. Genomic DNA amplification resulted in a 1063-bp fragment corresponding to a partial alpha-cardiac muscle actin gene containing exons 3 to 6. Southern blot analysis of the restriction-digested DNA revealed that the Nile tilapia genome contains multiple muscle actin isoforms. Although comparison of the nucleotide sequence, amino acid residues and exon-intron organization of the isolated actin gene with those of other vertebrates showed a high level of identity, diagnostic amino acid residues can still be correlated to distinct actin genes in fish species. |
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Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish speciesactin geneamino acid residuesgene structureNile tilapianucleotide sequenceOreochromis niloticusAn alpha actin gene segment, isolated from Nile tilapia (Oreochromis niloticus), was characterized by nucleotide sequencing, predicted amino acid sequence and Southern blot hybridization. Genomic DNA amplification resulted in a 1063-bp fragment corresponding to a partial alpha-cardiac muscle actin gene containing exons 3 to 6. Southern blot analysis of the restriction-digested DNA revealed that the Nile tilapia genome contains multiple muscle actin isoforms. Although comparison of the nucleotide sequence, amino acid residues and exon-intron organization of the isolated actin gene with those of other vertebrates showed a high level of identity, diagnostic amino acid residues can still be correlated to distinct actin genes in fish species.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista Instituto de Biociências Departamento de GenéticaUniversidade Estadual Paulista Instituto de Biociências Departamento de MorfologiaUniversidade Estadual Paulista Instituto de Biociências Departamento de GenéticaUniversidade Estadual Paulista Instituto de Biociências Departamento de MorfologiaSociedade Brasileira de GenéticaUniversidade Estadual Paulista (Unesp)Wasko, Adriane Pinto [UNESP]Severino, Fábio E. [UNESP]Presti, Flávia T. [UNESP]Poletto, Andréia B. [UNESP]Martins, Cesar [UNESP]2014-05-20T15:17:31Z2014-05-20T15:17:31Z2007-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1089-1092application/pdfhttp://dx.doi.org/10.1590/S1415-47572007000600010Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 4, p. 1089-1092, 2007.1415-4757http://hdl.handle.net/11449/3050210.1590/S1415-47572007000600010S1415-475720070006000102-s2.0-37649020453S1415-47572007000600010.pdf192686391737890988588006994253520000-0003-4600-03670000-0003-3534-974XSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-11-13T06:12:32Zoai:repositorio.unesp.br:11449/30502Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:35:07.544783Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species |
title |
Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species |
spellingShingle |
Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species Wasko, Adriane Pinto [UNESP] actin gene amino acid residues gene structure Nile tilapia nucleotide sequence Oreochromis niloticus |
title_short |
Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species |
title_full |
Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species |
title_fullStr |
Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species |
title_full_unstemmed |
Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species |
title_sort |
Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species |
author |
Wasko, Adriane Pinto [UNESP] |
author_facet |
Wasko, Adriane Pinto [UNESP] Severino, Fábio E. [UNESP] Presti, Flávia T. [UNESP] Poletto, Andréia B. [UNESP] Martins, Cesar [UNESP] |
author_role |
author |
author2 |
Severino, Fábio E. [UNESP] Presti, Flávia T. [UNESP] Poletto, Andréia B. [UNESP] Martins, Cesar [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Wasko, Adriane Pinto [UNESP] Severino, Fábio E. [UNESP] Presti, Flávia T. [UNESP] Poletto, Andréia B. [UNESP] Martins, Cesar [UNESP] |
dc.subject.por.fl_str_mv |
actin gene amino acid residues gene structure Nile tilapia nucleotide sequence Oreochromis niloticus |
topic |
actin gene amino acid residues gene structure Nile tilapia nucleotide sequence Oreochromis niloticus |
description |
An alpha actin gene segment, isolated from Nile tilapia (Oreochromis niloticus), was characterized by nucleotide sequencing, predicted amino acid sequence and Southern blot hybridization. Genomic DNA amplification resulted in a 1063-bp fragment corresponding to a partial alpha-cardiac muscle actin gene containing exons 3 to 6. Southern blot analysis of the restriction-digested DNA revealed that the Nile tilapia genome contains multiple muscle actin isoforms. Although comparison of the nucleotide sequence, amino acid residues and exon-intron organization of the isolated actin gene with those of other vertebrates showed a high level of identity, diagnostic amino acid residues can still be correlated to distinct actin genes in fish species. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-01-01 2014-05-20T15:17:31Z 2014-05-20T15:17:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1415-47572007000600010 Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 4, p. 1089-1092, 2007. 1415-4757 http://hdl.handle.net/11449/30502 10.1590/S1415-47572007000600010 S1415-47572007000600010 2-s2.0-37649020453 S1415-47572007000600010.pdf 1926863917378909 8858800699425352 0000-0003-4600-0367 0000-0003-3534-974X |
url |
http://dx.doi.org/10.1590/S1415-47572007000600010 http://hdl.handle.net/11449/30502 |
identifier_str_mv |
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 4, p. 1089-1092, 2007. 1415-4757 10.1590/S1415-47572007000600010 S1415-47572007000600010 2-s2.0-37649020453 S1415-47572007000600010.pdf 1926863917378909 8858800699425352 0000-0003-4600-0367 0000-0003-3534-974X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology 1.493 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1089-1092 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128829975166976 |