Comparison of the erythroimmunoadsorption by capture, immunoenzymatic and passive haemagglutination tests used in the diagnosis of neurocysticercosis

Detalhes bibliográficos
Autor(a) principal: Pialarissi, C. S. M.
Data de Publicação: 1995
Outros Autores: Nitrini, S. M. O. O.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista de Saúde Pública
Texto Completo: https://www.revistas.usp.br/rsp/article/view/24095
Resumo: Comparisons were made among the erythroimmunoadsorption by capture (C-EIA), the immunoenzymatic (ELISA) and passive haemaglutination (P.H.A.) tests used in the diagnosis of neurocysticercosis. The three tests, two of them routine by in diagnosis, and the third one (C-EIA) recently, standardised were compared for the detection of anti-Cysticercus cellulosae antibodies. In the three reactions the antigen utilized was raw saline extract (R.S.E) with a yield of 0.1, 1 and 10 µg protein/cavity for the C-EIA, ELISA and P.H.A tests, respectively. In a group of 58 patients with neurocysticercosis, the sensitivity to the ELISA, C-EIA and P.H.A tests was of 98.2%, 84.5% and 77,2%, respectively. In the control group of 85 individuals, healthy or with encephalopathies other than neurocysticercosis, the sensitivity was of 98.27%, 84.48% and 79.63%, respectively, and the specificity was of 94.1%, 95.3% and 91.8%. This order of choice should be followed, depending on the resources available.
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spelling Comparison of the erythroimmunoadsorption by capture, immunoenzymatic and passive haemagglutination tests used in the diagnosis of neurocysticercosis Comparação entre os testes de eritroimunoadsorção por captura, imunoenzimático e hemaglutinação passiva utilizados no diagnóstico da neurocisticercose Cisticercose^i1^sdiagnóstTécnicas de imunoadsorção^i1^sutilizaELISA^i1^sutilizaTestes de hemaglutinação^i1^sutilizaCysticercosis^i2^sdiagnoImmunosorbent techniques^i2^sutilizatEnzyme-linked immunosorbent assay^i2^sutilizatHemmagglutination tests^i2^sutilizat Comparisons were made among the erythroimmunoadsorption by capture (C-EIA), the immunoenzymatic (ELISA) and passive haemaglutination (P.H.A.) tests used in the diagnosis of neurocysticercosis. The three tests, two of them routine by in diagnosis, and the third one (C-EIA) recently, standardised were compared for the detection of anti-Cysticercus cellulosae antibodies. In the three reactions the antigen utilized was raw saline extract (R.S.E) with a yield of 0.1, 1 and 10 µg protein/cavity for the C-EIA, ELISA and P.H.A tests, respectively. In a group of 58 patients with neurocysticercosis, the sensitivity to the ELISA, C-EIA and P.H.A tests was of 98.2%, 84.5% and 77,2%, respectively. In the control group of 85 individuals, healthy or with encephalopathies other than neurocysticercosis, the sensitivity was of 98.27%, 84.48% and 79.63%, respectively, and the specificity was of 94.1%, 95.3% and 91.8%. This order of choice should be followed, depending on the resources available. Foi realizada a comparação entre os testes de eritroimunoadsorção por captura (EIAC), imunoenzimático (ELISA) e de hemaglutinação passiva (HAP) utilizados no diagnóstico da neurocisticercose. Foram comparados dois testes já anteriormente utilizados na rotina diagnóstica da neurocisticercose (ELISA e HAP) e um recentemente padronizado (EIAC) para a detecção de anticorpos anti-Cysticercus cellulosae. O antígeno empregado nos três testes foi o extrato salino bruto (ESB), com um rendimento de 0,1; 1 e 1µg proteína/cavidade para os testes EIAC, ELISA e HAP, respectivamente. Quando se analisou um grupo de 58 pacientes com neurocisticercose, a sensibilidade observada foi de 98,2%, 84,5% e 77,2% nos testes ELISA, EIAC e HAP, respectivamente, para um grupo controle de 85 indivíduos, saudáveis ou com outras encefalotipatias, mas sem neurocisticercose, a especificidade foi de 94,1%, 95,3%, 91,8% , respectivamente, nos testes. Esta ordem de escolha poderia ser obedecida na medida dos recursos disponíveis. Universidade de São Paulo. Faculdade de Saúde Pública1995-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rsp/article/view/2409510.1590/S0034-89101995000200005Revista de Saúde Pública; Vol. 29 No. 2 (1995); 115-119 Revista de Saúde Pública; Vol. 29 Núm. 2 (1995); 115-119 Revista de Saúde Pública; v. 29 n. 2 (1995); 115-119 1518-87870034-8910reponame:Revista de Saúde Públicainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/rsp/article/view/24095/26060Copyright (c) 2017 Revista de Saúde Públicainfo:eu-repo/semantics/openAccessPialarissi, C. S. M.Nitrini, S. M. O. O.2012-05-29T16:27:26Zoai:revistas.usp.br:article/24095Revistahttps://www.revistas.usp.br/rsp/indexONGhttps://www.revistas.usp.br/rsp/oairevsp@org.usp.br||revsp1@usp.br1518-87870034-8910opendoar:2012-05-29T16:27:26Revista de Saúde Pública - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Comparison of the erythroimmunoadsorption by capture, immunoenzymatic and passive haemagglutination tests used in the diagnosis of neurocysticercosis
Comparação entre os testes de eritroimunoadsorção por captura, imunoenzimático e hemaglutinação passiva utilizados no diagnóstico da neurocisticercose
title Comparison of the erythroimmunoadsorption by capture, immunoenzymatic and passive haemagglutination tests used in the diagnosis of neurocysticercosis
spellingShingle Comparison of the erythroimmunoadsorption by capture, immunoenzymatic and passive haemagglutination tests used in the diagnosis of neurocysticercosis
Pialarissi, C. S. M.
Cisticercose^i1^sdiagnóst
Técnicas de imunoadsorção^i1^sutiliza
ELISA^i1^sutiliza
Testes de hemaglutinação^i1^sutiliza
Cysticercosis^i2^sdiagno
Immunosorbent techniques^i2^sutilizat
Enzyme-linked immunosorbent assay^i2^sutilizat
Hemmagglutination tests^i2^sutilizat
title_short Comparison of the erythroimmunoadsorption by capture, immunoenzymatic and passive haemagglutination tests used in the diagnosis of neurocysticercosis
title_full Comparison of the erythroimmunoadsorption by capture, immunoenzymatic and passive haemagglutination tests used in the diagnosis of neurocysticercosis
title_fullStr Comparison of the erythroimmunoadsorption by capture, immunoenzymatic and passive haemagglutination tests used in the diagnosis of neurocysticercosis
title_full_unstemmed Comparison of the erythroimmunoadsorption by capture, immunoenzymatic and passive haemagglutination tests used in the diagnosis of neurocysticercosis
title_sort Comparison of the erythroimmunoadsorption by capture, immunoenzymatic and passive haemagglutination tests used in the diagnosis of neurocysticercosis
author Pialarissi, C. S. M.
author_facet Pialarissi, C. S. M.
Nitrini, S. M. O. O.
author_role author
author2 Nitrini, S. M. O. O.
author2_role author
dc.contributor.author.fl_str_mv Pialarissi, C. S. M.
Nitrini, S. M. O. O.
dc.subject.por.fl_str_mv Cisticercose^i1^sdiagnóst
Técnicas de imunoadsorção^i1^sutiliza
ELISA^i1^sutiliza
Testes de hemaglutinação^i1^sutiliza
Cysticercosis^i2^sdiagno
Immunosorbent techniques^i2^sutilizat
Enzyme-linked immunosorbent assay^i2^sutilizat
Hemmagglutination tests^i2^sutilizat
topic Cisticercose^i1^sdiagnóst
Técnicas de imunoadsorção^i1^sutiliza
ELISA^i1^sutiliza
Testes de hemaglutinação^i1^sutiliza
Cysticercosis^i2^sdiagno
Immunosorbent techniques^i2^sutilizat
Enzyme-linked immunosorbent assay^i2^sutilizat
Hemmagglutination tests^i2^sutilizat
description Comparisons were made among the erythroimmunoadsorption by capture (C-EIA), the immunoenzymatic (ELISA) and passive haemaglutination (P.H.A.) tests used in the diagnosis of neurocysticercosis. The three tests, two of them routine by in diagnosis, and the third one (C-EIA) recently, standardised were compared for the detection of anti-Cysticercus cellulosae antibodies. In the three reactions the antigen utilized was raw saline extract (R.S.E) with a yield of 0.1, 1 and 10 µg protein/cavity for the C-EIA, ELISA and P.H.A tests, respectively. In a group of 58 patients with neurocysticercosis, the sensitivity to the ELISA, C-EIA and P.H.A tests was of 98.2%, 84.5% and 77,2%, respectively. In the control group of 85 individuals, healthy or with encephalopathies other than neurocysticercosis, the sensitivity was of 98.27%, 84.48% and 79.63%, respectively, and the specificity was of 94.1%, 95.3% and 91.8%. This order of choice should be followed, depending on the resources available.
publishDate 1995
dc.date.none.fl_str_mv 1995-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rsp/article/view/24095
10.1590/S0034-89101995000200005
url https://www.revistas.usp.br/rsp/article/view/24095
identifier_str_mv 10.1590/S0034-89101995000200005
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rsp/article/view/24095/26060
dc.rights.driver.fl_str_mv Copyright (c) 2017 Revista de Saúde Pública
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Revista de Saúde Pública
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Saúde Pública
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Saúde Pública
dc.source.none.fl_str_mv Revista de Saúde Pública; Vol. 29 No. 2 (1995); 115-119
Revista de Saúde Pública; Vol. 29 Núm. 2 (1995); 115-119
Revista de Saúde Pública; v. 29 n. 2 (1995); 115-119
1518-8787
0034-8910
reponame:Revista de Saúde Pública
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Revista de Saúde Pública
collection Revista de Saúde Pública
repository.name.fl_str_mv Revista de Saúde Pública - Universidade de São Paulo (USP)
repository.mail.fl_str_mv revsp@org.usp.br||revsp1@usp.br
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