Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomona
Autor(a) principal: | |
---|---|
Data de Publicação: | 1993 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/52024 |
Resumo: | After inoculating L. interrogans serovar pomona in 75 primiparous hamsters (Mesocricetus auratus), the invasiveness of leptospires into lhe ovaries and lhe ability in causing ovary morphologic alterations were investigated by means of microscopic examination and bacterial isolation. For this purpose, 75 hamsters were inoculated with 0.5 ml of virulent strain containing 30-40 leptospires by the microscopic field and the other 15 hamsters were held as the uninfected controls. Signs and symptoms (prostration, tachypnea, rufled hair, jaundice, and nasal, bucal and perineal hemorrage) were detected in all inoculated animals. The animals were killed in the agonic state of the illness, which were done through 4th and 7th day post inoculation. The ovaries were taken asseptically during the necropsies, thoroughly washed using the sterile phosphate buffered saline, in order to eliminate the possible external contamination. The fresh ovary samples were submitted to the dark field direct microscopic examination. After the formalin fixation, the specimens were stained by means of histopathologic techniques using the Levaditi and Hematoxylin Eosin stains. The ovary smears were also examined by the direct fluorescent antibody technique andlhe bacterial isolation was carried out in the Fletcher’s medium. The dark field direct microscopic examination was found tobe less sensitive in demonstrating the presence of leptospiresin the ovaries. In those specimens stained by the Lcvadititechnique, leptospires were visualized in different ovaryinternal structures, involving the interspace, pellucid zone andin the inner ovules. Through the histopathologic examination,typical morphologic alterations resembling acute infiamatoryprocess were found in 57% of ovaries examined. |
id |
USP-49_6b9e14e0945507b53d5a6e54d0fb54f2 |
---|---|
oai_identifier_str |
oai:revistas.usp.br:article/52024 |
network_acronym_str |
USP-49 |
network_name_str |
Brazilian Journal of Veterinary Research and Animal Science |
repository_id_str |
https://www.revistas.usp.br/bjvras/index |
spelling |
Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomonaInvestigation on the presence of leptospires in ovaries of hamsters experimentally infected whith Leptospiras interrogans serovar pomonaLeptospirosisLeptospira interrogans (isolation)Ovaries (hamsters)LeptospiroseLeptospira interrogans (isolamento)Ovários (hamster)After inoculating L. interrogans serovar pomona in 75 primiparous hamsters (Mesocricetus auratus), the invasiveness of leptospires into lhe ovaries and lhe ability in causing ovary morphologic alterations were investigated by means of microscopic examination and bacterial isolation. For this purpose, 75 hamsters were inoculated with 0.5 ml of virulent strain containing 30-40 leptospires by the microscopic field and the other 15 hamsters were held as the uninfected controls. Signs and symptoms (prostration, tachypnea, rufled hair, jaundice, and nasal, bucal and perineal hemorrage) were detected in all inoculated animals. The animals were killed in the agonic state of the illness, which were done through 4th and 7th day post inoculation. The ovaries were taken asseptically during the necropsies, thoroughly washed using the sterile phosphate buffered saline, in order to eliminate the possible external contamination. The fresh ovary samples were submitted to the dark field direct microscopic examination. After the formalin fixation, the specimens were stained by means of histopathologic techniques using the Levaditi and Hematoxylin Eosin stains. The ovary smears were also examined by the direct fluorescent antibody technique andlhe bacterial isolation was carried out in the Fletcher’s medium. The dark field direct microscopic examination was found tobe less sensitive in demonstrating the presence of leptospiresin the ovaries. In those specimens stained by the Lcvadititechnique, leptospires were visualized in different ovaryinternal structures, involving the interspace, pellucid zone andin the inner ovules. Through the histopathologic examination,typical morphologic alterations resembling acute infiamatoryprocess were found in 57% of ovaries examined.De 90 hamsters primíparas com 80 a 120 gramas de peso vivo, 75 foram inoculadas com a dose individual de 0,5 ml de estirpe virulenta do sorotipo pomona (30 a 40 leptospiras ativas por campo microscópico, no aumento de 200 vezes) e as 15 remanescentes constituíram o grupo testemunho não infectado. Todos os animais tratados com leptospiras apresentaram os sinais da infecção (prostração, taquipnéia, eriçamento do pelame, icterícia e hemorragias nasal, bucal e perineal) e foram sacrificados por ocasião da fase agônica da doença, situada entre o quarto e o sétimo dia da inoculação. Nesta oportunidade, os ovários foram colhidos em condições assépticas e submetidos à técnica de visualização de leptospiras (exame direto em microscopia de campo escuro, coloração argêntica de Levaditi e reação de imunofluorescência direta), cultivo em meio de Fletcher e exame histopatológico (coloração de hematoxilina e eosina). A ocorrência de uma possível contaminação estabelecida durante a retirada dos ovários foi investigada através da lavagem em solução salina tamponada estéril. As leptospiras foram demonstradas em todos os ovários do grupo de animais experimentalmente inoculados (lavados e não lavados), através da coloração de Levaditi, reação imunofluorescência direta e também no cultivo em meio de Fletcher. O exame direto em microscopia de campo escuro mostrou ser uma técnica muito pouco sensível. O exame das preparações submetidas à coloração argêntica possibilitou a visualização de leptospiras em diferentes estruturas dos ovários, incluindo: o interstício, a zona pelúcida e o interior dos óvulos. Os exames histopatológicos permitiram observar as alterações morfológicas típicas de um processo inflamatório agudo em 57% dos ovários examinados.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia1993-12-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/5202410.11606/issn.1678-4456.bjvras.1993.52024Brazilian Journal of Veterinary Research and Animal Science; Vol. 30 Núm. 2 (1993); 129-135Brazilian Journal of Veterinary Research and Animal Science; Vol. 30 No. 2 (1993); 129-135Brazilian Journal of Veterinary Research and Animal Science; v. 30 n. 2 (1993); 129-135Brazilian Journal of Veterinary Research and Animal Science; V. 30 N. 2 (1993); 129-1351678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/52024/56074Camargo, Claudio Roberto de AlmeidaVasconcellos, Silvio ArrudaNürmberger Júnior, RodolfoPassos, Estevão de CamargoMorais, Zenaíde Maria deVisintin, José Antonioinfo:eu-repo/semantics/openAccess2020-06-23T04:54:00Zoai:revistas.usp.br:article/52024Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:26.726569Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomona Investigation on the presence of leptospires in ovaries of hamsters experimentally infected whith Leptospiras interrogans serovar pomona |
title |
Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomona |
spellingShingle |
Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomona Camargo, Claudio Roberto de Almeida Leptospirosis Leptospira interrogans (isolation) Ovaries (hamsters) Leptospirose Leptospira interrogans (isolamento) Ovários (hamster) |
title_short |
Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomona |
title_full |
Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomona |
title_fullStr |
Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomona |
title_full_unstemmed |
Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomona |
title_sort |
Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomona |
author |
Camargo, Claudio Roberto de Almeida |
author_facet |
Camargo, Claudio Roberto de Almeida Vasconcellos, Silvio Arruda Nürmberger Júnior, Rodolfo Passos, Estevão de Camargo Morais, Zenaíde Maria de Visintin, José Antonio |
author_role |
author |
author2 |
Vasconcellos, Silvio Arruda Nürmberger Júnior, Rodolfo Passos, Estevão de Camargo Morais, Zenaíde Maria de Visintin, José Antonio |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Camargo, Claudio Roberto de Almeida Vasconcellos, Silvio Arruda Nürmberger Júnior, Rodolfo Passos, Estevão de Camargo Morais, Zenaíde Maria de Visintin, José Antonio |
dc.subject.por.fl_str_mv |
Leptospirosis Leptospira interrogans (isolation) Ovaries (hamsters) Leptospirose Leptospira interrogans (isolamento) Ovários (hamster) |
topic |
Leptospirosis Leptospira interrogans (isolation) Ovaries (hamsters) Leptospirose Leptospira interrogans (isolamento) Ovários (hamster) |
description |
After inoculating L. interrogans serovar pomona in 75 primiparous hamsters (Mesocricetus auratus), the invasiveness of leptospires into lhe ovaries and lhe ability in causing ovary morphologic alterations were investigated by means of microscopic examination and bacterial isolation. For this purpose, 75 hamsters were inoculated with 0.5 ml of virulent strain containing 30-40 leptospires by the microscopic field and the other 15 hamsters were held as the uninfected controls. Signs and symptoms (prostration, tachypnea, rufled hair, jaundice, and nasal, bucal and perineal hemorrage) were detected in all inoculated animals. The animals were killed in the agonic state of the illness, which were done through 4th and 7th day post inoculation. The ovaries were taken asseptically during the necropsies, thoroughly washed using the sterile phosphate buffered saline, in order to eliminate the possible external contamination. The fresh ovary samples were submitted to the dark field direct microscopic examination. After the formalin fixation, the specimens were stained by means of histopathologic techniques using the Levaditi and Hematoxylin Eosin stains. The ovary smears were also examined by the direct fluorescent antibody technique andlhe bacterial isolation was carried out in the Fletcher’s medium. The dark field direct microscopic examination was found tobe less sensitive in demonstrating the presence of leptospiresin the ovaries. In those specimens stained by the Lcvadititechnique, leptospires were visualized in different ovaryinternal structures, involving the interspace, pellucid zone andin the inner ovules. Through the histopathologic examination,typical morphologic alterations resembling acute infiamatoryprocess were found in 57% of ovaries examined. |
publishDate |
1993 |
dc.date.none.fl_str_mv |
1993-12-02 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/52024 10.11606/issn.1678-4456.bjvras.1993.52024 |
url |
https://www.revistas.usp.br/bjvras/article/view/52024 |
identifier_str_mv |
10.11606/issn.1678-4456.bjvras.1993.52024 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/52024/56074 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 30 Núm. 2 (1993); 129-135 Brazilian Journal of Veterinary Research and Animal Science; Vol. 30 No. 2 (1993); 129-135 Brazilian Journal of Veterinary Research and Animal Science; v. 30 n. 2 (1993); 129-135 Brazilian Journal of Veterinary Research and Animal Science; V. 30 N. 2 (1993); 129-135 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051563776147456 |