wFlu: Characterization and Evaluation of a Native Wolbachia from the Mosquito Aedes fluviatilis as a Potential Vector Control Agent

Detalhes bibliográficos
Autor(a) principal: Baton, Luke Anthony
Data de Publicação: 2013
Outros Autores: Pacidônio, Etiene Casagrande, Gonçalves, Daniela da Silva, Moreira, Luciano Andrade
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30204
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, Minas Gerais, Brazil
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spelling Baton, Luke AnthonyPacidônio, Etiene CasagrandeGonçalves, Daniela da SilvaMoreira, Luciano Andrade2018-11-26T17:40:21Z2018-11-26T17:40:21Z2013BATON, Luke Anthony et al. wFlu: Characterization and Evaluation of a Native Wolbachia from the Mosquito Aedes fluviatilis as a Potential Vector Control Agent. PLoS One, v. 8, n.10, p. e59619, 20131932-6203https://www.arca.fiocruz.br/handle/icict/3020410.1371/journal.pone.0059619engPublic Library of SciencemalariaWolbachiaAedes fluviatilismalariaWolbachiaAedes fluviatiliswFlu: Characterization and Evaluation of a Native Wolbachia from the Mosquito Aedes fluviatilis as a Potential Vector Control Agentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, Minas Gerais, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, Minas Gerais, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, Minas Gerais, BrazilThere is currently considerable interest and practical progress in using the endosymbiotic bacteria Wolbachia as a vector control agent for human vector-borne diseases. Such vector control strategies may require the introduction of multiple, different Wolbachia strains into target vector populations, necessitating the identification and characterization of appropriate endosymbiont variants. Here, we report preliminary characterization of wFlu, a native Wolbachia from the neotropical mosquito Aedes fluviatilis, and evaluate its potential as a vector control agent by confirming its ability to cause cytoplasmic incompatibility, and measuring its effect on three parameters determining host fitness (survival, fecundity and fertility), as well as vector competence (susceptibility) for pathogen infection. Using an aposymbiotic strain of Ae. fluviatilis cured of its native Wolbachia by antibiotic treatment, we show that in its natural host wFlu causes incomplete, but high levels of, unidirectional cytoplasmic incompatibility, has high rates of maternal transmission, and no detectable fitness costs, indicating a high capacity to rapidly spread through host populations. However, wFlu does not inhibit, and even enhances, oocyst infection with the avian malaria parasite Plasmodium gallinaceum. The stage- and sex-specific density of wFlu was relatively low, and with limited tissue distribution, consistent with the lack of virulence and pathogen interference/symbiont-mediated protection observed. Unexpectedly, the density of wFlu was also shown to be specifically-reduced in the ovaries after bloodfeeding Ae. fluviatilis. Overall, our observations indicate that the Wolbachia strain wFlu has the potential to be used as a vector control agent, and suggests that appreciable mutualistic coevolution has occurred between this endosymbiont and its natural host. Future work will be needed to determine whether wFlu has virulent host effects and/or exhibits pathogen interference when artificially-transfected to the novel mosquito hosts that are the vectors of human pathogens.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/30204/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALwFlu.pdfwFlu.pdfapplication/pdf10059164https://www.arca.fiocruz.br/bitstream/icict/30204/2/wFlu.pdf0899b5239e7cede26720e2666112eee5MD52TEXTwFlu.pdf.txtwFlu.pdf.txtExtracted texttext/plain121https://www.arca.fiocruz.br/bitstream/icict/30204/3/wFlu.pdf.txt1199c0df180b0550afa7f12f1e3ac34eMD53icict/302042019-06-19 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dc.title.pt_BR.fl_str_mv wFlu: Characterization and Evaluation of a Native Wolbachia from the Mosquito Aedes fluviatilis as a Potential Vector Control Agent
title wFlu: Characterization and Evaluation of a Native Wolbachia from the Mosquito Aedes fluviatilis as a Potential Vector Control Agent
spellingShingle wFlu: Characterization and Evaluation of a Native Wolbachia from the Mosquito Aedes fluviatilis as a Potential Vector Control Agent
Baton, Luke Anthony
malaria
Wolbachia
Aedes fluviatilis
malaria
Wolbachia
Aedes fluviatilis
title_short wFlu: Characterization and Evaluation of a Native Wolbachia from the Mosquito Aedes fluviatilis as a Potential Vector Control Agent
title_full wFlu: Characterization and Evaluation of a Native Wolbachia from the Mosquito Aedes fluviatilis as a Potential Vector Control Agent
title_fullStr wFlu: Characterization and Evaluation of a Native Wolbachia from the Mosquito Aedes fluviatilis as a Potential Vector Control Agent
title_full_unstemmed wFlu: Characterization and Evaluation of a Native Wolbachia from the Mosquito Aedes fluviatilis as a Potential Vector Control Agent
title_sort wFlu: Characterization and Evaluation of a Native Wolbachia from the Mosquito Aedes fluviatilis as a Potential Vector Control Agent
author Baton, Luke Anthony
author_facet Baton, Luke Anthony
Pacidônio, Etiene Casagrande
Gonçalves, Daniela da Silva
Moreira, Luciano Andrade
author_role author
author2 Pacidônio, Etiene Casagrande
Gonçalves, Daniela da Silva
Moreira, Luciano Andrade
author2_role author
author
author
dc.contributor.author.fl_str_mv Baton, Luke Anthony
Pacidônio, Etiene Casagrande
Gonçalves, Daniela da Silva
Moreira, Luciano Andrade
dc.subject.other.pt_BR.fl_str_mv malaria
Wolbachia
Aedes fluviatilis
topic malaria
Wolbachia
Aedes fluviatilis
malaria
Wolbachia
Aedes fluviatilis
dc.subject.en.pt_BR.fl_str_mv malaria
Wolbachia
Aedes fluviatilis
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, Minas Gerais, Brazil
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2018-11-26T17:40:21Z
dc.date.available.fl_str_mv 2018-11-26T17:40:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv BATON, Luke Anthony et al. wFlu: Characterization and Evaluation of a Native Wolbachia from the Mosquito Aedes fluviatilis as a Potential Vector Control Agent. PLoS One, v. 8, n.10, p. e59619, 2013
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30204
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0059619
identifier_str_mv BATON, Luke Anthony et al. wFlu: Characterization and Evaluation of a Native Wolbachia from the Mosquito Aedes fluviatilis as a Potential Vector Control Agent. PLoS One, v. 8, n.10, p. e59619, 2013
1932-6203
10.1371/journal.pone.0059619
url https://www.arca.fiocruz.br/handle/icict/30204
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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