Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily

Detalhes bibliográficos
Autor(a) principal: Matsunaga, James
Data de Publicação: 2003
Outros Autores: Barocchi, Michele A, Croda, Julio Henrique Rosa, Young, Tracy A, Sanchez, Yolanda, Siqueira, Isadora Cristina de, Bolin, Carole A, Reis, Mitermayer Galvão dos, Riley, Lee Woodland, Haake, David A, Ko, Albert Icksang
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7561
Resumo: Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, USA / Department of Medicine. UCLA School of Medicine. Los Angeles, CA, USA.
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spelling Matsunaga, JamesBarocchi, Michele ACroda, Julio Henrique RosaYoung, Tracy ASanchez, YolandaSiqueira, Isadora Cristina deBolin, Carole AReis, Mitermayer Galvão dosRiley, Lee WoodlandHaake, David AKo, Albert Icksang2014-04-30T17:19:56Z2014-04-30T17:19:56Z2003MATSUNAGA, J. et al. Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily. Molecular Microbiology, v. 49, n. 4, p. 929-945, 2003.0950-382Xhttps://www.arca.fiocruz.br/handle/icict/7561engNational Institute of HealthPathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamilyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleVeterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, USA / Department of Medicine. UCLA School of Medicine. Los Angeles, CA, USA.Division of Infectious Diseases and Immunity. School of Public Health. University of California. Berkeley Berkeley, CA, USA.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, USA.Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, USA / Department of Medicine. UCLA School of Medicine. Los Angeles, CA, USA.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.College of Veterinary Medicine. Michigan State University. East Lansing, MI, USA.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Division of Infectious Diseases and Immunity. School of Public Health. University of California. Berkeley Berkeley, CA, USA.Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, USA / Department of Medicine. UCLA School of Medicine. Los Angeles, CA, USA.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Division of International Medicine and Infectious Diseases. Weill Medical College of Cornell University. New York, NY, USA.Proteins with bacterial immunoglobulin-like (Big) domains, such as the Yersinia pseudotuberculosis invasin and Escherichia coli intimin, are surface-expressed proteins that mediate host mammalian cell invasion or attachment. Here, we report the identification and characterization of a new family of Big domain proteins, referred to as Lig (leptospiral Ig-like) proteins, in pathogenic Leptospira. Screening of L. interrogans and L. kirschneri expression libraries with sera from leptospirosis patients identified 13 lambda phage clones that encode tandem repeats of the 90 amino acid Big domain. Two lig genes, designated ligA and ligB, and one pseudogene, ligC, were identified. The ligA and ligB genes encode amino-terminal lipoprotein signal peptides followed by 10 or 11 Big domain repeats and, in the case of ligB, a unique carboxy-terminal non-repeat domain. The organization of ligC is similar to that of ligB but contains mutations that disrupt the reading frame. The lig sequences are present in pathogenic but not saprophytic Leptospira species. LigA and LigB are expressed by a variety of virulent leptospiral strains. Loss of Lig protein and RNA transcript expression is correlated with the observed loss of virulence during culture attenuation of pathogenic strains. High-pressure freeze substitution followed by immunocytochemical electron microscopy confirmed that the Lig proteins were localized to the bacterial surface. Immunoblot studies with patient sera found that the Lig proteins are a major antigen recognized during the acute host infection. These observations demonstrate that the Lig proteins are a newly identified surface protein of pathogenic Leptospira, which by analogy to other bacterial immunoglobulin superfamily virulence factors, may play a role in host cell attachment and invasion during leptospiral pathogenesis.Anticorpos Antibacterianos/genéticaProteínas de Bactérias/metabolismoLeptospira/genéticaLeptospira/metabolismoProteínas de Membrana/metabolismoSequência de AminoácidosAnimaisAnticorpos Antibacterianos/químicaProteínas de Bactérias/químicaRegulação Bacteriana da Expressão GênicaHumanosImunoglobulinas/genéticaLeptospira/imunologiaProteínas de Membrana/químicaModelos MolecularesDados de Sequência MolecularEstrutura Terciária de Proteínainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALMatsunaga J Pathogenic Leptospira....pdfMatsunaga J Pathogenic Leptospira....pdfapplication/pdf1203467https://www.arca.fiocruz.br/bitstream/icict/7561/1/Matsunaga%20J%20Pathogenic%20Leptospira....pdf293db660c4d6910bda034909fe19bb75MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7561/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTMatsunaga J Pathogenic Leptospira....pdf.txtMatsunaga J Pathogenic Leptospira....pdf.txtExtracted texttext/plain78130https://www.arca.fiocruz.br/bitstream/icict/7561/5/Matsunaga%20J%20Pathogenic%20Leptospira....pdf.txt9660570e77f96a80fc9a10c035f625c1MD55THUMBNAILMatsunaga J Pathogenic Leptospira....pdf.jpgMatsunaga J Pathogenic Leptospira....pdf.jpgGenerated Thumbnailimage/jpeg2382https://www.arca.fiocruz.br/bitstream/icict/7561/4/Matsunaga%20J%20Pathogenic%20Leptospira....pdf.jpg045650177e8e2a31a5da6bc4b472e3c4MD54icict/75612023-03-15 14:33:19.353oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:19Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily
title Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily
spellingShingle Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily
Matsunaga, James
Anticorpos Antibacterianos/genética
Proteínas de Bactérias/metabolismo
Leptospira/genética
Leptospira/metabolismo
Proteínas de Membrana/metabolismo
Sequência de Aminoácidos
Animais
Anticorpos Antibacterianos/química
Proteínas de Bactérias/química
Regulação Bacteriana da Expressão Gênica
Humanos
Imunoglobulinas/genética
Leptospira/imunologia
Proteínas de Membrana/química
Modelos Moleculares
Dados de Sequência Molecular
Estrutura Terciária de Proteína
title_short Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily
title_full Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily
title_fullStr Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily
title_full_unstemmed Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily
title_sort Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily
author Matsunaga, James
author_facet Matsunaga, James
Barocchi, Michele A
Croda, Julio Henrique Rosa
Young, Tracy A
Sanchez, Yolanda
Siqueira, Isadora Cristina de
Bolin, Carole A
Reis, Mitermayer Galvão dos
Riley, Lee Woodland
Haake, David A
Ko, Albert Icksang
author_role author
author2 Barocchi, Michele A
Croda, Julio Henrique Rosa
Young, Tracy A
Sanchez, Yolanda
Siqueira, Isadora Cristina de
Bolin, Carole A
Reis, Mitermayer Galvão dos
Riley, Lee Woodland
Haake, David A
Ko, Albert Icksang
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Matsunaga, James
Barocchi, Michele A
Croda, Julio Henrique Rosa
Young, Tracy A
Sanchez, Yolanda
Siqueira, Isadora Cristina de
Bolin, Carole A
Reis, Mitermayer Galvão dos
Riley, Lee Woodland
Haake, David A
Ko, Albert Icksang
dc.subject.decs.pt_BR.fl_str_mv Anticorpos Antibacterianos/genética
Proteínas de Bactérias/metabolismo
Leptospira/genética
Leptospira/metabolismo
Proteínas de Membrana/metabolismo
Sequência de Aminoácidos
Animais
Anticorpos Antibacterianos/química
Proteínas de Bactérias/química
Regulação Bacteriana da Expressão Gênica
Humanos
Imunoglobulinas/genética
Leptospira/imunologia
Proteínas de Membrana/química
Modelos Moleculares
Dados de Sequência Molecular
Estrutura Terciária de Proteína
topic Anticorpos Antibacterianos/genética
Proteínas de Bactérias/metabolismo
Leptospira/genética
Leptospira/metabolismo
Proteínas de Membrana/metabolismo
Sequência de Aminoácidos
Animais
Anticorpos Antibacterianos/química
Proteínas de Bactérias/química
Regulação Bacteriana da Expressão Gênica
Humanos
Imunoglobulinas/genética
Leptospira/imunologia
Proteínas de Membrana/química
Modelos Moleculares
Dados de Sequência Molecular
Estrutura Terciária de Proteína
description Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, USA / Department of Medicine. UCLA School of Medicine. Los Angeles, CA, USA.
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2014-04-30T17:19:56Z
dc.date.available.fl_str_mv 2014-04-30T17:19:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MATSUNAGA, J. et al. Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily. Molecular Microbiology, v. 49, n. 4, p. 929-945, 2003.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7561
dc.identifier.issn.none.fl_str_mv 0950-382X
identifier_str_mv MATSUNAGA, J. et al. Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily. Molecular Microbiology, v. 49, n. 4, p. 929-945, 2003.
0950-382X
url https://www.arca.fiocruz.br/handle/icict/7561
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv National Institute of Health
publisher.none.fl_str_mv National Institute of Health
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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