Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA

Detalhes bibliográficos
Autor(a) principal: Shigunov, Patrícia
Data de Publicação: 2018
Outros Autores: Balvedi, Lucas Titton, Santos, Marlon Dias Mariano, Herai, Roberto H., Aguiar, Alessandra Melo de, Dallagiovanna, Bruno
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31812
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
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spelling Shigunov, PatríciaBalvedi, Lucas TittonSantos, Marlon Dias MarianoHerai, Roberto H.Aguiar, Alessandra Melo deDallagiovanna, Bruno2019-02-21T15:11:16Z2019-02-21T15:11:16Z2018SHIGUNOV, Patrícia et al. Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA. Scientific Reports, v. 8, n. 8418, 2018.2045-2322https://www.arca.fiocruz.br/handle/icict/3181210.1038/s41598-018-26533-yporNature ResearchProteínas HedgehogAdult Stem CellsGene ExpressionHedgehog ProteinsAdipose TissueProtein BiosynthesisRNA, MessengerCélulas Madre AdultasExpresión GénicaTejido AdiposoBiosíntesis de ProteínasARN MensajeroCélulas-Tronco AdultasExpressão GênicaTecido AdiposoBiossíntese de ProteínasRNA MensageiroCrosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNAinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Grupo de Espectrometria de Massas Computacional e Proteômica. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Escola de Medicina. Programa de Graduação em Ciências da Saúde. Divisão de Terapia Celular. Laboratório Experimental Multiuso. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Adult stem cells are considered promising candidates for cellular therapies due to their capacity to differentiate and self-renew. Differentiation leads to changes in the metabolism, structure, and gene expression patterns of cells. Hedgehog is one of the pathways that is involved in the enhancement of osteogenesis and chondrogenesis in adult stem cells, but its mechanisms are poorly understood. In this study, we treated adipose tissue-derived stem cells (ADSC) with two well-characterized drugs, purmorphamine (Hedgehog pathway activator) and cyclopamine (Hedgehog pathway inhibitor), and identified mRNAs associated with polysomes in each treatment group to determine the post transcriptional genetic networks governed by the Hedgehog pathway. Activation of the Hedgehog pathway by purmorphamine results in significant upregulation of mRNAs associated with cellular communication and signal transduction. Furthermore, our experiments show that cyclopamine acts late downregulating GLI1 expression in ADSCs but promotes the upregulation of mRNAs associated with energy pathways and metabolism at early times. Through in silico analysis, we identified some miRNAs, such as miR-355, that could regulate these mRNAs association with polysomes and thereby modulate the Hedgehog pathway. Our results suggest that activation of the Hedgehog pathway by purmorphamine also results in a negative regulation of mRNAs in the protein translation machinery.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/31812/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALs41598-018-265.pdfs41598-018-265.pdfapplication/pdf2868417https://www.arca.fiocruz.br/bitstream/icict/31812/2/s41598-018-265.pdf2aafcd778e3da201b5a4ff508d60ebbcMD52TEXTs41598-018-265.pdf.txts41598-018-265.pdf.txtExtracted texttext/plain50203https://www.arca.fiocruz.br/bitstream/icict/31812/3/s41598-018-265.pdf.txt253bf4769fcba320c9f3ec0031415179MD53icict/318122019-02-22 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dc.title.pt_BR.fl_str_mv Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA
title Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA
spellingShingle Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA
Shigunov, Patrícia
Proteínas Hedgehog
Adult Stem Cells
Gene Expression
Hedgehog Proteins
Adipose Tissue
Protein Biosynthesis
RNA, Messenger
Células Madre Adultas
Expresión Génica
Tejido Adiposo
Biosíntesis de Proteínas
ARN Mensajero
Células-Tronco Adultas
Expressão Gênica
Tecido Adiposo
Biossíntese de Proteínas
RNA Mensageiro
title_short Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA
title_full Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA
title_fullStr Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA
title_full_unstemmed Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA
title_sort Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA
author Shigunov, Patrícia
author_facet Shigunov, Patrícia
Balvedi, Lucas Titton
Santos, Marlon Dias Mariano
Herai, Roberto H.
Aguiar, Alessandra Melo de
Dallagiovanna, Bruno
author_role author
author2 Balvedi, Lucas Titton
Santos, Marlon Dias Mariano
Herai, Roberto H.
Aguiar, Alessandra Melo de
Dallagiovanna, Bruno
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Shigunov, Patrícia
Balvedi, Lucas Titton
Santos, Marlon Dias Mariano
Herai, Roberto H.
Aguiar, Alessandra Melo de
Dallagiovanna, Bruno
dc.subject.other.pt_BR.fl_str_mv Proteínas Hedgehog
topic Proteínas Hedgehog
Adult Stem Cells
Gene Expression
Hedgehog Proteins
Adipose Tissue
Protein Biosynthesis
RNA, Messenger
Células Madre Adultas
Expresión Génica
Tejido Adiposo
Biosíntesis de Proteínas
ARN Mensajero
Células-Tronco Adultas
Expressão Gênica
Tecido Adiposo
Biossíntese de Proteínas
RNA Mensageiro
dc.subject.en.pt_BR.fl_str_mv Adult Stem Cells
Gene Expression
Hedgehog Proteins
Adipose Tissue
Protein Biosynthesis
RNA, Messenger
dc.subject.es.pt_BR.fl_str_mv Células Madre Adultas
Expresión Génica
Tejido Adiposo
Biosíntesis de Proteínas
ARN Mensajero
dc.subject.decs.pt_BR.fl_str_mv Células-Tronco Adultas
Expressão Gênica
Tecido Adiposo
Biossíntese de Proteínas
RNA Mensageiro
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-02-21T15:11:16Z
dc.date.available.fl_str_mv 2019-02-21T15:11:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SHIGUNOV, Patrícia et al. Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA. Scientific Reports, v. 8, n. 8418, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31812
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-018-26533-y
identifier_str_mv SHIGUNOV, Patrícia et al. Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA. Scientific Reports, v. 8, n. 8418, 2018.
2045-2322
10.1038/s41598-018-26533-y
url https://www.arca.fiocruz.br/handle/icict/31812
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/31812/1/license.txt
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