Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/31812 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. |
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Shigunov, PatríciaBalvedi, Lucas TittonSantos, Marlon Dias MarianoHerai, Roberto H.Aguiar, Alessandra Melo deDallagiovanna, Bruno2019-02-21T15:11:16Z2019-02-21T15:11:16Z2018SHIGUNOV, Patrícia et al. Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA. Scientific Reports, v. 8, n. 8418, 2018.2045-2322https://www.arca.fiocruz.br/handle/icict/3181210.1038/s41598-018-26533-yporNature ResearchProteínas HedgehogAdult Stem CellsGene ExpressionHedgehog ProteinsAdipose TissueProtein BiosynthesisRNA, MessengerCélulas Madre AdultasExpresión GénicaTejido AdiposoBiosíntesis de ProteínasARN MensajeroCélulas-Tronco AdultasExpressão GênicaTecido AdiposoBiossíntese de ProteínasRNA MensageiroCrosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNAinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Grupo de Espectrometria de Massas Computacional e Proteômica. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Escola de Medicina. Programa de Graduação em Ciências da Saúde. Divisão de Terapia Celular. Laboratório Experimental Multiuso. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Adult stem cells are considered promising candidates for cellular therapies due to their capacity to differentiate and self-renew. Differentiation leads to changes in the metabolism, structure, and gene expression patterns of cells. Hedgehog is one of the pathways that is involved in the enhancement of osteogenesis and chondrogenesis in adult stem cells, but its mechanisms are poorly understood. In this study, we treated adipose tissue-derived stem cells (ADSC) with two well-characterized drugs, purmorphamine (Hedgehog pathway activator) and cyclopamine (Hedgehog pathway inhibitor), and identified mRNAs associated with polysomes in each treatment group to determine the post transcriptional genetic networks governed by the Hedgehog pathway. Activation of the Hedgehog pathway by purmorphamine results in significant upregulation of mRNAs associated with cellular communication and signal transduction. Furthermore, our experiments show that cyclopamine acts late downregulating GLI1 expression in ADSCs but promotes the upregulation of mRNAs associated with energy pathways and metabolism at early times. Through in silico analysis, we identified some miRNAs, such as miR-355, that could regulate these mRNAs association with polysomes and thereby modulate the Hedgehog pathway. Our results suggest that activation of the Hedgehog pathway by purmorphamine also results in a negative regulation of mRNAs in the protein translation machinery.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/31812/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALs41598-018-265.pdfs41598-018-265.pdfapplication/pdf2868417https://www.arca.fiocruz.br/bitstream/icict/31812/2/s41598-018-265.pdf2aafcd778e3da201b5a4ff508d60ebbcMD52TEXTs41598-018-265.pdf.txts41598-018-265.pdf.txtExtracted texttext/plain50203https://www.arca.fiocruz.br/bitstream/icict/31812/3/s41598-018-265.pdf.txt253bf4769fcba320c9f3ec0031415179MD53icict/318122019-02-22 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dc.title.pt_BR.fl_str_mv |
Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA |
title |
Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA |
spellingShingle |
Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA Shigunov, Patrícia Proteínas Hedgehog Adult Stem Cells Gene Expression Hedgehog Proteins Adipose Tissue Protein Biosynthesis RNA, Messenger Células Madre Adultas Expresión Génica Tejido Adiposo Biosíntesis de Proteínas ARN Mensajero Células-Tronco Adultas Expressão Gênica Tecido Adiposo Biossíntese de Proteínas RNA Mensageiro |
title_short |
Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA |
title_full |
Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA |
title_fullStr |
Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA |
title_full_unstemmed |
Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA |
title_sort |
Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA |
author |
Shigunov, Patrícia |
author_facet |
Shigunov, Patrícia Balvedi, Lucas Titton Santos, Marlon Dias Mariano Herai, Roberto H. Aguiar, Alessandra Melo de Dallagiovanna, Bruno |
author_role |
author |
author2 |
Balvedi, Lucas Titton Santos, Marlon Dias Mariano Herai, Roberto H. Aguiar, Alessandra Melo de Dallagiovanna, Bruno |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Shigunov, Patrícia Balvedi, Lucas Titton Santos, Marlon Dias Mariano Herai, Roberto H. Aguiar, Alessandra Melo de Dallagiovanna, Bruno |
dc.subject.other.pt_BR.fl_str_mv |
Proteínas Hedgehog |
topic |
Proteínas Hedgehog Adult Stem Cells Gene Expression Hedgehog Proteins Adipose Tissue Protein Biosynthesis RNA, Messenger Células Madre Adultas Expresión Génica Tejido Adiposo Biosíntesis de Proteínas ARN Mensajero Células-Tronco Adultas Expressão Gênica Tecido Adiposo Biossíntese de Proteínas RNA Mensageiro |
dc.subject.en.pt_BR.fl_str_mv |
Adult Stem Cells Gene Expression Hedgehog Proteins Adipose Tissue Protein Biosynthesis RNA, Messenger |
dc.subject.es.pt_BR.fl_str_mv |
Células Madre Adultas Expresión Génica Tejido Adiposo Biosíntesis de Proteínas ARN Mensajero |
dc.subject.decs.pt_BR.fl_str_mv |
Células-Tronco Adultas Expressão Gênica Tecido Adiposo Biossíntese de Proteínas RNA Mensageiro |
description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2019-02-21T15:11:16Z |
dc.date.available.fl_str_mv |
2019-02-21T15:11:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SHIGUNOV, Patrícia et al. Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA. Scientific Reports, v. 8, n. 8418, 2018. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/31812 |
dc.identifier.issn.pt_BR.fl_str_mv |
2045-2322 |
dc.identifier.doi.none.fl_str_mv |
10.1038/s41598-018-26533-y |
identifier_str_mv |
SHIGUNOV, Patrícia et al. Crosstalk between Hedgehog pathway and energy pathways in human adipose-derived stem cells: A deep sequencing analysis of polysome-associated RNA. Scientific Reports, v. 8, n. 8418, 2018. 2045-2322 10.1038/s41598-018-26533-y |
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https://www.arca.fiocruz.br/handle/icict/31812 |
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Nature Research |
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Nature Research |
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