Reversion of gene expression alterations in hearts of mice with chronic chagasic cardiomyopathy after transplantation of bone marrow cells.
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/8579 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil |
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Soares, Milena Botelho PereiraLima, Ricardo Santana deSouza, Bruno Solano de FreitasVasconcelos, Juliana FragaRocha, Leonardo LimaSantos, Ricardo Ribeiro dosIacobas, SandaGoldenberg, Regina Coeli dos SantosLisanti, Michael PIacobas, Dumitru AndreiTanowitz, Herbert BernardSpray, David ConoverCarvalho, Antonio Carlos Campos de2014-10-13T17:55:09Z2014-10-13T17:55:09Z2011SOARES, M. B. P. et al. Reversion of gene expression alterations in hearts of mice with chronic chagasic cardiomyopathy after transplantation of bone marrow cells. Cell Cycle, v. 10, n. 9, p. 1448-1455, 2011.1551-4005https://www.arca.fiocruz.br/handle/icict/857910.4161/cc.10.9.15487engLandes BioscienceReversion of gene expression alterations in hearts of mice with chronic chagasic cardiomyopathy after transplantation of bone marrow cells.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilUniversidade Federal do Vale do São Francisco. Departamento de Medicina. Petrolina, PE, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilAlbert Einstein College of Medicine. Dominick P. Purpura Department of Neuroscience. Bronx, NY, USAUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, BrasilThomas Jefferson University. Department of Stem Cell Biology and Regenerative Medicine. Kimmel Cancer Center. Philadelphia, PA, USAAlbert Einstein College of Medicine. Dominick P. Purpura Department of Neuroscience. Bronx, NY, USAAlbert Einstein College of Medicine. Department of Medicine. Bronx, NY, USA / Albert Einstein College of Medicine. Department of Pathology. Bronx, NY, USAAlbert Einstein College of Medicine. Dominick P. Purpura Department of Neuroscience. Bronx, NY, USA / Albert Einstein College of Medicine. Department of Medicine. Bronx, NY, USAAlbert Einstein College of Medicine. Dominick P. Purpura Department of Neuroscience. Bronx, NY, USA / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / 8Instituto Nacional de Cardiologia. Rio de Janeiro, RJ, BrasilChronic chagasic cardiomyopathy is a leading cause of heart failure in Latin American countries, being associated with intense inflammatory response and fibrosis. We have previously shown that bone marrow mononuclear cell (BMC) transplantation improves inflammation, fibrosis, and ventricular diameter in hearts of mice with chronic Chagas disease. Here we investigated the transcriptomic recovery induced by BMC therapy by comparing the heart transcriptomes of control, chagasic, and BMC transplanted mice. Out of the 9390 unique genes quantified in all samples, 1702 had their expression altered in chronic chagasic hearts compared to those of normal mice. Major categories of significantly upregulated genes were related to inflammation, fibrosis and immune responses, while genes involved in mitochondrion function were downregulated. When BMC-treated chagasic hearts were compared to infected mice, 96% of the alterations detected in infected hearts were restored to normal levels, although an additional 109 genes were altered by treatment. Transcriptomic recovery, a new measure that considers both resotrative and side effects of treatment, was remarkably high (84%). Immunofluorescence and morphometric analyses confirmed the effects of BMC therapy in the pattern of inflammatory-immune response and expression of adhesion molecules. In conclusion, by using large-scale gene profiling for unbiased assessment of therapeutic efficacy we demonstrate immunomodulatory effects of BMC therapy in chronic chagasic cardiomyopathy and identify potentially relevant factors involved in the pathogenesis of the disease that may provide new therapeutic targetsChagas diseaseTrypanosoma cruziCardiomyopathyCell transplantationMicroarrayInflammationFibrosisTransplante de Medula Óssea/patologiaCardiomiopatia Chagásica/genéticaRegulação da Expressão Gênica/imunologiaMiocárdio/imunologiaTrypanosoma cruzi/imunologiaAnimaisTransplante de Medula Óssea/imunologiaCardiomiopatia Chagásica/imunologiaCardiomiopatia Chagásica/terapiaDoença CrônicaModelos Animais de DoençasFemininoFibroseGalectina 3/genéticaPerfilação da Expressão Gênica/métodosMasculinoCamundongosCamundongos Endogâmicos C57BLMiocárdio/metabolismoAnálise de Sequência com Séries de OligonucleotídeosTrypanosoma cruzi/classificaçãoFator de von Willebrand/genéticainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8579/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALSoares MBP Reversion of gene....pdfSoares MBP Reversion of gene....pdfapplication/pdf3296336https://www.arca.fiocruz.br/bitstream/icict/8579/2/Soares%20MBP%20Reversion%20of%20gene....pdf0ff5c89e3bdaa634a9dd1795b27f7c4fMD52TEXTSoares MBP Reversion of gene....pdf.txtSoares MBP Reversion of gene....pdf.txtExtracted texttext/plain33893https://www.arca.fiocruz.br/bitstream/icict/8579/3/Soares%20MBP%20Reversion%20of%20gene....pdf.txte6bae477ea2bf38ff871927d999d31b4MD53icict/85792023-03-15 14:33:24.004oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:24Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Reversion of gene expression alterations in hearts of mice with chronic chagasic cardiomyopathy after transplantation of bone marrow cells. |
| title |
Reversion of gene expression alterations in hearts of mice with chronic chagasic cardiomyopathy after transplantation of bone marrow cells. |
| spellingShingle |
Reversion of gene expression alterations in hearts of mice with chronic chagasic cardiomyopathy after transplantation of bone marrow cells. Soares, Milena Botelho Pereira Chagas disease Trypanosoma cruzi Cardiomyopathy Cell transplantation Microarray Inflammation Fibrosis Transplante de Medula Óssea/patologia Cardiomiopatia Chagásica/genética Regulação da Expressão Gênica/imunologia Miocárdio/imunologia Trypanosoma cruzi/imunologia Animais Transplante de Medula Óssea/imunologia Cardiomiopatia Chagásica/imunologia Cardiomiopatia Chagásica/terapia Doença Crônica Modelos Animais de Doenças Feminino Fibrose Galectina 3/genética Perfilação da Expressão Gênica/métodos Masculino Camundongos Camundongos Endogâmicos C57BL Miocárdio/metabolismo Análise de Sequência com Séries de Oligonucleotídeos Trypanosoma cruzi/classificação Fator de von Willebrand/genética |
| title_short |
Reversion of gene expression alterations in hearts of mice with chronic chagasic cardiomyopathy after transplantation of bone marrow cells. |
| title_full |
Reversion of gene expression alterations in hearts of mice with chronic chagasic cardiomyopathy after transplantation of bone marrow cells. |
| title_fullStr |
Reversion of gene expression alterations in hearts of mice with chronic chagasic cardiomyopathy after transplantation of bone marrow cells. |
| title_full_unstemmed |
Reversion of gene expression alterations in hearts of mice with chronic chagasic cardiomyopathy after transplantation of bone marrow cells. |
| title_sort |
Reversion of gene expression alterations in hearts of mice with chronic chagasic cardiomyopathy after transplantation of bone marrow cells. |
| author |
Soares, Milena Botelho Pereira |
| author_facet |
Soares, Milena Botelho Pereira Lima, Ricardo Santana de Souza, Bruno Solano de Freitas Vasconcelos, Juliana Fraga Rocha, Leonardo Lima Santos, Ricardo Ribeiro dos Iacobas, Sanda Goldenberg, Regina Coeli dos Santos Lisanti, Michael P Iacobas, Dumitru Andrei Tanowitz, Herbert Bernard Spray, David Conover Carvalho, Antonio Carlos Campos de |
| author_role |
author |
| author2 |
Lima, Ricardo Santana de Souza, Bruno Solano de Freitas Vasconcelos, Juliana Fraga Rocha, Leonardo Lima Santos, Ricardo Ribeiro dos Iacobas, Sanda Goldenberg, Regina Coeli dos Santos Lisanti, Michael P Iacobas, Dumitru Andrei Tanowitz, Herbert Bernard Spray, David Conover Carvalho, Antonio Carlos Campos de |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Soares, Milena Botelho Pereira Lima, Ricardo Santana de Souza, Bruno Solano de Freitas Vasconcelos, Juliana Fraga Rocha, Leonardo Lima Santos, Ricardo Ribeiro dos Iacobas, Sanda Goldenberg, Regina Coeli dos Santos Lisanti, Michael P Iacobas, Dumitru Andrei Tanowitz, Herbert Bernard Spray, David Conover Carvalho, Antonio Carlos Campos de |
| dc.subject.en.pt_BR.fl_str_mv |
Chagas disease Trypanosoma cruzi Cardiomyopathy Cell transplantation Microarray Inflammation Fibrosis |
| topic |
Chagas disease Trypanosoma cruzi Cardiomyopathy Cell transplantation Microarray Inflammation Fibrosis Transplante de Medula Óssea/patologia Cardiomiopatia Chagásica/genética Regulação da Expressão Gênica/imunologia Miocárdio/imunologia Trypanosoma cruzi/imunologia Animais Transplante de Medula Óssea/imunologia Cardiomiopatia Chagásica/imunologia Cardiomiopatia Chagásica/terapia Doença Crônica Modelos Animais de Doenças Feminino Fibrose Galectina 3/genética Perfilação da Expressão Gênica/métodos Masculino Camundongos Camundongos Endogâmicos C57BL Miocárdio/metabolismo Análise de Sequência com Séries de Oligonucleotídeos Trypanosoma cruzi/classificação Fator de von Willebrand/genética |
| dc.subject.decs.pt_BR.fl_str_mv |
Transplante de Medula Óssea/patologia Cardiomiopatia Chagásica/genética Regulação da Expressão Gênica/imunologia Miocárdio/imunologia Trypanosoma cruzi/imunologia Animais Transplante de Medula Óssea/imunologia Cardiomiopatia Chagásica/imunologia Cardiomiopatia Chagásica/terapia Doença Crônica Modelos Animais de Doenças Feminino Fibrose Galectina 3/genética Perfilação da Expressão Gênica/métodos Masculino Camundongos Camundongos Endogâmicos C57BL Miocárdio/metabolismo Análise de Sequência com Séries de Oligonucleotídeos Trypanosoma cruzi/classificação Fator de von Willebrand/genética |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil |
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2011 |
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2011 |
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2014-10-13T17:55:09Z |
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2014-10-13T17:55:09Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
SOARES, M. B. P. et al. Reversion of gene expression alterations in hearts of mice with chronic chagasic cardiomyopathy after transplantation of bone marrow cells. Cell Cycle, v. 10, n. 9, p. 1448-1455, 2011. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/8579 |
| dc.identifier.issn.none.fl_str_mv |
1551-4005 |
| dc.identifier.doi.none.fl_str_mv |
10.4161/cc.10.9.15487 |
| identifier_str_mv |
SOARES, M. B. P. et al. Reversion of gene expression alterations in hearts of mice with chronic chagasic cardiomyopathy after transplantation of bone marrow cells. Cell Cycle, v. 10, n. 9, p. 1448-1455, 2011. 1551-4005 10.4161/cc.10.9.15487 |
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https://www.arca.fiocruz.br/handle/icict/8579 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Landes Bioscience |
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Landes Bioscience |
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