Leishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages.

Detalhes bibliográficos
Autor(a) principal: Almeida, Taís Fontoura de
Data de Publicação: 2012
Outros Autores: Palma, Luana Carneiro, Mendez, Lucas C, Dutra, Alberto Augusto Noronha, Veras, Patrícia Sampaio Tavares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7728
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
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spelling Almeida, Taís Fontoura dePalma, Luana CarneiroMendez, Lucas CDutra, Alberto Augusto NoronhaVeras, Patrícia Sampaio Tavares2014-05-30T11:44:46Z2014-05-30T11:44:46Z2012ALMEIDA, T. F. et al. Leishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages. Parasite Immunology, v. 34, n. 10, p. 492-498, 2012.1365-3024https://www.arca.fiocruz.br/handle/icict/772810.1111/j.1365-3024.2012.01384.xengBlackwell Publishing LtdLeishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilCBA mouse macrophages effectively control Leishmania major infection, yet are permissive to Leishmania amazonensis. It has been established that some Leishmania species are destroyed by reactive oxygen species (ROS). However, other species of Leishmania exhibit resistance to ROS or even down-modulate ROS production. We hypothesized that L. amazonensis-infected macrophages reduce ROS production soon after parasite-cell interaction. Employing a highly sensitive analysis technique based on chemiluminescence, the production of superoxide (O(·-)(2)) and hydrogen peroxide (H(2)O(2)) by L. major- or L. amazonensis-infected CBA macrophages were measured. L. major induces macrophages to release levels of (O(·-)(2)) 3·5 times higher than in uninfected cells. This (O(·-)(2)) production is partially dependent on NADPH oxidase (NOX) type 2. The level of accumulated H(2)O(2) is 20 times higher in L. major-than in L. amazonensis-infected cells. Furthermore, macrophages stimulated with L. amazonensis release amounts of ROS similar to uninfected cells. These findings support previous studies showing that CBA macrophages are effective in controlling L. major infection by a mechanism dependent on both (O(·-)(2)) production and H(2)O(2) generation. Furthermore, these data reinforce the notion that L. amazonensis survive inside CBA macrophages by reducing ROS production during the phagocytic process.LeishmaniaMacrophageReactive oxygen intermediatesLeishmania mexicana/imunologiaMacrófagos/imunologiaMacrófagos/parasitologiaEspécies de Oxigênio Reativas/metabolismoAnimaisPeróxido de Hidrogênio/análiseLeishmania/imunologiaLeishmania major/imunologiaMedições LuminescentesCamundongosCamundongos Endogâmicos CBANADPH Oxidase/metabolismoSuperóxidos/análiseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALAlmeida TF Leishmania amazonensis....pdfAlmeida TF Leishmania amazonensis....pdfapplication/pdf549441https://www.arca.fiocruz.br/bitstream/icict/7728/1/Almeida%20TF%20Leishmania%20amazonensis....pdfbe4373bbc97f2d7b75430f8b8f393253MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7728/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTAlmeida TF Leishmania amazonensis....pdf.txtAlmeida TF Leishmania amazonensis....pdf.txtExtracted texttext/plain36170https://www.arca.fiocruz.br/bitstream/icict/7728/5/Almeida%20TF%20Leishmania%20amazonensis....pdf.txt69910995880fb3f241d6c739495c74c5MD55THUMBNAILAlmeida TF Leishmania amazonensis....pdf.jpgAlmeida TF Leishmania amazonensis....pdf.jpgGenerated Thumbnailimage/jpeg2182https://www.arca.fiocruz.br/bitstream/icict/7728/4/Almeida%20TF%20Leishmania%20amazonensis....pdf.jpgafb77d65c042ac449ef0fc83f83041b7MD54icict/77282023-03-15 14:33:00.697oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Leishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages.
title Leishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages.
spellingShingle Leishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages.
Almeida, Taís Fontoura de
Leishmania
Macrophage
Reactive oxygen intermediates
Leishmania mexicana/imunologia
Macrófagos/imunologia
Macrófagos/parasitologia
Espécies de Oxigênio Reativas/metabolismo
Animais
Peróxido de Hidrogênio/análise
Leishmania/imunologia
Leishmania major/imunologia
Medições Luminescentes
Camundongos
Camundongos Endogâmicos CBA
NADPH Oxidase/metabolismo
Superóxidos/análise
title_short Leishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages.
title_full Leishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages.
title_fullStr Leishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages.
title_full_unstemmed Leishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages.
title_sort Leishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages.
author Almeida, Taís Fontoura de
author_facet Almeida, Taís Fontoura de
Palma, Luana Carneiro
Mendez, Lucas C
Dutra, Alberto Augusto Noronha
Veras, Patrícia Sampaio Tavares
author_role author
author2 Palma, Luana Carneiro
Mendez, Lucas C
Dutra, Alberto Augusto Noronha
Veras, Patrícia Sampaio Tavares
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Almeida, Taís Fontoura de
Palma, Luana Carneiro
Mendez, Lucas C
Dutra, Alberto Augusto Noronha
Veras, Patrícia Sampaio Tavares
dc.subject.en.pt_BR.fl_str_mv Leishmania
Macrophage
Reactive oxygen intermediates
topic Leishmania
Macrophage
Reactive oxygen intermediates
Leishmania mexicana/imunologia
Macrófagos/imunologia
Macrófagos/parasitologia
Espécies de Oxigênio Reativas/metabolismo
Animais
Peróxido de Hidrogênio/análise
Leishmania/imunologia
Leishmania major/imunologia
Medições Luminescentes
Camundongos
Camundongos Endogâmicos CBA
NADPH Oxidase/metabolismo
Superóxidos/análise
dc.subject.decs.pt_BR.fl_str_mv Leishmania mexicana/imunologia
Macrófagos/imunologia
Macrófagos/parasitologia
Espécies de Oxigênio Reativas/metabolismo
Animais
Peróxido de Hidrogênio/análise
Leishmania/imunologia
Leishmania major/imunologia
Medições Luminescentes
Camundongos
Camundongos Endogâmicos CBA
NADPH Oxidase/metabolismo
Superóxidos/análise
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-05-30T11:44:46Z
dc.date.available.fl_str_mv 2014-05-30T11:44:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA, T. F. et al. Leishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages. Parasite Immunology, v. 34, n. 10, p. 492-498, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7728
dc.identifier.issn.none.fl_str_mv 1365-3024
dc.identifier.doi.none.fl_str_mv 10.1111/j.1365-3024.2012.01384.x
identifier_str_mv ALMEIDA, T. F. et al. Leishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages. Parasite Immunology, v. 34, n. 10, p. 492-498, 2012.
1365-3024
10.1111/j.1365-3024.2012.01384.x
url https://www.arca.fiocruz.br/handle/icict/7728
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Blackwell Publishing Ltd
publisher.none.fl_str_mv Blackwell Publishing Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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