Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy

Detalhes bibliográficos
Autor(a) principal: Gutierrez, Brenda Celeste
Data de Publicação: 2022
Outros Autores: Ancarola, Maria Eugenia, Volpato-Rossi, Izadora, Marcilla, Antonio, Ramirez, Marcel Ivan, Rosenzvit, Mara Cecilia, Cucher, Marcela, Poncini, Carolina Verónica
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/56442
Resumo: Instituto de Investigaciones en Microbiología y Parasitología Médicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Buenos Aires, Argentina.
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spelling Gutierrez, Brenda CelesteAncarola, Maria EugeniaVolpato-Rossi, IzadoraMarcilla, AntonioRamirez, Marcel IvanRosenzvit, Mara CeciliaCucher, MarcelaPoncini, Carolina Verónica2023-01-12T11:46:37Z2023-01-12T11:46:37Z2022GUTIERREZ, Brenda Celeste et al. Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy. Frontiers, v. 12, n. 980817, p. 1–14 , 2022.2235-2988https://www.arca.fiocruz.br/handle/icict/5644210.3389/fcimb.2022.980817porFrontiers MediaExtracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto de Investigaciones en Microbiología y Parasitología Médicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Buenos Aires, Argentina.Instituto de Investigaciones en Microbiología y Parasitología Médicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Buenos Aires, Argentina.Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Departamento de Farmácia y Tecnologia Farmacêutica y Parasitología. Universitat de Valencia. Valencia, Spain.Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Instituto de Investigaciones en Microbiología y Parasitología Médicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Buenos Aires, Argentina.Instituto de Investigaciones en Microbiología y Parasitología Médicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Buenos Aires, Argentina.Instituto de Investigaciones en Microbiología y Parasitología Médicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Buenos Aires, Argentina. / Departamento de Microbiología. Facultad de Medicina. Universidad de Buenos Aires (UBA). Buenos Aires, Argentina.Extracellular vesicles (EVs) include a heterogeneous group of particles. Microvesicles, apoptotic bodies and exosomes are the most characterized vesicles. They can be distinguished by their size, morphology, origin and molecular composition. To date, increasing studies demonstrate that EVs mediate intercellular communication. EVs reach considerable interest in the scientific community due to their role in diverse processes including antigenpresentation, stimulation of anti-tumoral immune responses, tolerogenic or inflammatory effects. In pathogens, EV shedding is well described in fungi, bacteria, protozoan and helminths parasites. For Trypanosoma cruzi EV liberation and protein composition was previously described. Dendritic cells (DCs), among other cells, are key players promoting the immune response against pathogens and also maintaining self-tolerance. In previous reports we have demonstrate that T. cruzi downregulates DCs immunogenicity in vitro and in vivo. Here we analyze EVs from the in vitro interaction between blood circulating trypomastigotes (Tp) and bone-marrow-derived DCs. We found that Tp incremented the number and the size of EVs in cultures with DCs. EVs displayed some exosome markers and intracellular RNA. Protein analysis demonstrated that the parasite changes the DC protein-EV profile. We observed that EVs from the interaction of Tp-DCs were easily captured by unstimulated-DCs in comparison with EVs from DCs cultured without the parasite, and also modified the activation status of LPS-stimulated DCs.Dendritic CellsCélulas DendríticasCellules dendritiquesTrypanosoma cruziinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/56442/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALExtracellular-Vesciles-fcimbok.pdfExtracellular-Vesciles-fcimbok.pdfapplication/pdf3108228https://www.arca.fiocruz.br/bitstream/icict/56442/2/Extracellular-Vesciles-fcimbok.pdf2f954ca15f9212af6d8350bc4ecd32b9MD52icict/564422023-01-12 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dc.title.en_US.fl_str_mv Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy
title Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy
spellingShingle Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy
Gutierrez, Brenda Celeste
Dendritic Cells
Células Dendríticas
Cellules dendritiques
Trypanosoma cruzi
title_short Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy
title_full Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy
title_fullStr Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy
title_full_unstemmed Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy
title_sort Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy
author Gutierrez, Brenda Celeste
author_facet Gutierrez, Brenda Celeste
Ancarola, Maria Eugenia
Volpato-Rossi, Izadora
Marcilla, Antonio
Ramirez, Marcel Ivan
Rosenzvit, Mara Cecilia
Cucher, Marcela
Poncini, Carolina Verónica
author_role author
author2 Ancarola, Maria Eugenia
Volpato-Rossi, Izadora
Marcilla, Antonio
Ramirez, Marcel Ivan
Rosenzvit, Mara Cecilia
Cucher, Marcela
Poncini, Carolina Verónica
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gutierrez, Brenda Celeste
Ancarola, Maria Eugenia
Volpato-Rossi, Izadora
Marcilla, Antonio
Ramirez, Marcel Ivan
Rosenzvit, Mara Cecilia
Cucher, Marcela
Poncini, Carolina Verónica
dc.subject.en.en_US.fl_str_mv Dendritic Cells
topic Dendritic Cells
Células Dendríticas
Cellules dendritiques
Trypanosoma cruzi
dc.subject.es.en_US.fl_str_mv Células Dendríticas
dc.subject.fr.en_US.fl_str_mv Cellules dendritiques
dc.subject.decs.en_US.fl_str_mv Trypanosoma cruzi
description Instituto de Investigaciones en Microbiología y Parasitología Médicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Buenos Aires, Argentina.
publishDate 2022
dc.date.issued.fl_str_mv 2022
dc.date.accessioned.fl_str_mv 2023-01-12T11:46:37Z
dc.date.available.fl_str_mv 2023-01-12T11:46:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GUTIERREZ, Brenda Celeste et al. Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy. Frontiers, v. 12, n. 980817, p. 1–14 , 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/56442
dc.identifier.issn.en_US.fl_str_mv 2235-2988
dc.identifier.doi.none.fl_str_mv 10.3389/fcimb.2022.980817
identifier_str_mv GUTIERREZ, Brenda Celeste et al. Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy. Frontiers, v. 12, n. 980817, p. 1–14 , 2022.
2235-2988
10.3389/fcimb.2022.980817
url https://www.arca.fiocruz.br/handle/icict/56442
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/56442/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/56442/2/Extracellular-Vesciles-fcimbok.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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