The presence of Tregs does not preclude immunity to reinfection with Leishmania braziliensis

Detalhes bibliográficos
Autor(a) principal: Falcão, Sarah de Athayde Couto
Data de Publicação: 2012
Outros Autores: Moura, Tatiana Rodrigues de, Clarêncio, Jorge, Brodskyn, Claudia Ida, Barral, Aldina Maria Prado, Oliveira, Camila Indiani de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7257
http://dx.doi.org/10.1016/j.ijpara.2012.05.006
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
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spelling Falcão, Sarah de Athayde CoutoMoura, Tatiana Rodrigues deClarêncio, JorgeBrodskyn, Claudia IdaBarral, Aldina Maria PradoOliveira, Camila Indiani de2013-11-25T13:42:50Z2013-11-25T13:42:50Z2012FALCÃO, S. C. et al. The presence of Tregs does not preclude immunity to reinfection with Leishmania braziliensis. International Journal of Parasitology, v.42, n. 8, p.771-780, 2012.0020-7519https://www.arca.fiocruz.br/handle/icict/7257http://dx.doi.org/10.1016/j.ijpara.2012.05.006engElsevier LtdThe presence of Tregs does not preclude immunity to reinfection with Leishmania braziliensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. BrasilLeishmania spp. cause a broad spectrum of diseases collectively known as leishmaniasis. Leishmania braziliensis is the main etiological agent of American cutaneous leishmaniasis and mucocutaneous leishmaniasis. During experimental infection with L. braziliensis, BALB/c mice develop an adaptive immune response that is associated with lesion healing and, in parallel, parasite persistence within draining lymph nodes (dLNs). In the Leishmania major model of cutaneous leishmaniasis, regulatory T cells (Tregs) play an important role in immune regulation, preventing pathological immune responses but at the same time precluding sterile cure. In this study we investigated the role of Tregs during experimental infection with L. braziliensis. CD4+CD25+ T cells were detected throughout the duration of clinical disease both at the ear and in dLNs of infected mice. These cells expressed Treg markers such as glucocorticoid-induced TNF-receptor-related protein (GITR), the a chain of the aeb7 integrin (CD103), and the forkhead/winged helix transcription factor, Foxp3, and were able to suppress the proliferation of CD4+CD25 cells. Importantly, a high frequency of Foxp3+ cells accumulated at the site of infection and in dLNs. We next investigated the outcome of a reinfection with L. braziliensis in terms of Treg distribution and disease reactivation. Interestingly, a secondary inoculation with L. braziliensis did not preclude an efficient recall response to L. braziliensis at a distal site, despite the presence of Tregs. Within dLNs, reinfection did not promote parasite dissemination or a differential recruitment of either CD4+CD25+Foxp3+ or CD4+IL-10+ T cells. On the contrary, parasites were mainly detected in the LN draining the primary infection site where a high frequency of CD4+IFN-c+ T cells was also present. Collectively these data show that during experimental infection, Tregs are present in healed mice but this population does not compromise an effective immune response upon reinfection with L. braziliensis.Leishmania braziliensisTregCD4+CD25+info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALFalcao Sarah C The presence....pdfFalcao Sarah C The presence....pdfapplication/pdf1178741https://www.arca.fiocruz.br/bitstream/icict/7257/1/Falcao%20Sarah%20C%20The%20presence....pdfb3bdc8d97d71c98db69b74045fd87057MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7257/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTFalcao Sarah C The presence....pdf.txtFalcao Sarah C The presence....pdf.txtExtracted texttext/plain47952https://www.arca.fiocruz.br/bitstream/icict/7257/5/Falcao%20Sarah%20C%20The%20presence....pdf.txtb16aa701e60711ffc9f103d2d3465129MD55THUMBNAILFalcao Sarah C The presence....pdf.jpgFalcao Sarah C The presence....pdf.jpgGenerated Thumbnailimage/jpeg2311https://www.arca.fiocruz.br/bitstream/icict/7257/4/Falcao%20Sarah%20C%20The%20presence....pdf.jpg8244ff01c88d2444e7b83f946996678dMD54icict/72572023-03-15 14:32:54.115oai:www.arca.fiocruz.br:icict/7257TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:54Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv The presence of Tregs does not preclude immunity to reinfection with Leishmania braziliensis
title The presence of Tregs does not preclude immunity to reinfection with Leishmania braziliensis
spellingShingle The presence of Tregs does not preclude immunity to reinfection with Leishmania braziliensis
Falcão, Sarah de Athayde Couto
Leishmania braziliensis
Treg
CD4+CD25+
title_short The presence of Tregs does not preclude immunity to reinfection with Leishmania braziliensis
title_full The presence of Tregs does not preclude immunity to reinfection with Leishmania braziliensis
title_fullStr The presence of Tregs does not preclude immunity to reinfection with Leishmania braziliensis
title_full_unstemmed The presence of Tregs does not preclude immunity to reinfection with Leishmania braziliensis
title_sort The presence of Tregs does not preclude immunity to reinfection with Leishmania braziliensis
author Falcão, Sarah de Athayde Couto
author_facet Falcão, Sarah de Athayde Couto
Moura, Tatiana Rodrigues de
Clarêncio, Jorge
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
author_role author
author2 Moura, Tatiana Rodrigues de
Clarêncio, Jorge
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Falcão, Sarah de Athayde Couto
Moura, Tatiana Rodrigues de
Clarêncio, Jorge
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
dc.subject.en.pt_BR.fl_str_mv Leishmania braziliensis
Treg
CD4+CD25+
topic Leishmania braziliensis
Treg
CD4+CD25+
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2013-11-25T13:42:50Z
dc.date.available.fl_str_mv 2013-11-25T13:42:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv FALCÃO, S. C. et al. The presence of Tregs does not preclude immunity to reinfection with Leishmania braziliensis. International Journal of Parasitology, v.42, n. 8, p.771-780, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7257
dc.identifier.issn.none.fl_str_mv 0020-7519
dc.identifier.doi.none.fl_str_mv http://dx.doi.org/10.1016/j.ijpara.2012.05.006
identifier_str_mv FALCÃO, S. C. et al. The presence of Tregs does not preclude immunity to reinfection with Leishmania braziliensis. International Journal of Parasitology, v.42, n. 8, p.771-780, 2012.
0020-7519
url https://www.arca.fiocruz.br/handle/icict/7257
http://dx.doi.org/10.1016/j.ijpara.2012.05.006
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