Leishmania Lipophosphoglycan Triggers Caspase-11 and the Non-canonical Activation of the NLRP3 Inflammasome

Detalhes bibliográficos
Autor(a) principal: Carvalho, Renan Villanova Homem de
Data de Publicação: 2019
Outros Autores: Andrade, Warrison A., Souza Júnior, Djalma Lima, Dilucca, Marisa, Oliveira, Caroline Vitória Fernandes Souza de, Wang, Kun, Nogueira, Paula Monalisa, Rugani, Jeronimo Marteleto Nunes, Soares, Rodrigo Pedro Pinto, Beverley, Stephen M., Shao, Feng, Zamboni, Dario Simoes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36581
Resumo: Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, Brasil.
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spelling Carvalho, Renan Villanova Homem deAndrade, Warrison A.Souza Júnior, Djalma LimaDilucca, MarisaOliveira, Caroline Vitória Fernandes Souza deWang, KunNogueira, Paula MonalisaRugani, Jeronimo Marteleto NunesSoares, Rodrigo Pedro PintoBeverley, Stephen M.Shao, FengZamboni, Dario Simoes2019-10-18T17:51:32Z2019-10-18T17:51:32Z2019CARVALHO, Renan Villanova Homem de et al. Leishmania Lipophosphoglycan Triggers Caspase-11 and the Non-canonical Activation of the NLRP3 Inflammasome. Cell Reports, v. 26, n. 2, p. 429-437, 2019.2211-1247https://www.arca.fiocruz.br/handle/icict/3658110.1016/j.celrep.2018.12.047engElsevierLeishmania Lipophosphoglycan Triggers Caspase-11 and the Non-canonical Activation of the NLRP3 Inflammasomeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, Brasil.National Institute of Biological Sciences. Beijing, China.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Department of Molecular Microbiology. Washington University School of Medicine. Saint Louis, MO, USA.National Institute of Biological Sciences. Beijing, China.Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, Brasil.Activation of the NLRP3 inflammasome by Leishmania parasites is critical for the outcome of leishmaniasis, a disease that affects millions of people worldwide. We investigate the mechanisms involved in NLRP3 activation and demonstrate that caspase-11 (CASP11) is activated in response to infection by Leishmania species and triggers the non-canonical activation of NLRP3. This process accounts for host resistance to infection in macrophages and in vivo. We identify the parasite membrane glycoconjugate lipophosphoglycan (LPG) as the molecule involved in CASP11 activation. Cytosolic delivery of LPG in macrophages triggers CASP11 activation, and infections performed with Lpg1(-/-) parasites reduce CASP11/NLRP3 activation. Unlike bacterial LPS, purified LPG does not activate mouse CASP11 (or human Casp4) in vitro, suggesting the participation of additional molecules for LPG-mediated CASP11 activation. Our data identify a parasite molecule involved in CASP11 activation, thereby establishing the mechanisms underlying inflammasome activation in response to Leishmania species.ParasitesLeishmaniainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/36581/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALLeishmania Lipophosphoglycan Triggers.pdfLeishmania Lipophosphoglycan Triggers.pdfapplication/pdf6570102https://www.arca.fiocruz.br/bitstream/icict/36581/2/Leishmania%20Lipophosphoglycan%20Triggers.pdf1cc17d11c4468270e12be56a2360905eMD52TEXTLeishmania Lipophosphoglycan Triggers.pdf.txtLeishmania Lipophosphoglycan Triggers.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Leishmania Lipophosphoglycan Triggers Caspase-11 and the Non-canonical Activation of the NLRP3 Inflammasome
title Leishmania Lipophosphoglycan Triggers Caspase-11 and the Non-canonical Activation of the NLRP3 Inflammasome
spellingShingle Leishmania Lipophosphoglycan Triggers Caspase-11 and the Non-canonical Activation of the NLRP3 Inflammasome
Carvalho, Renan Villanova Homem de
Parasites
Leishmania
title_short Leishmania Lipophosphoglycan Triggers Caspase-11 and the Non-canonical Activation of the NLRP3 Inflammasome
title_full Leishmania Lipophosphoglycan Triggers Caspase-11 and the Non-canonical Activation of the NLRP3 Inflammasome
title_fullStr Leishmania Lipophosphoglycan Triggers Caspase-11 and the Non-canonical Activation of the NLRP3 Inflammasome
title_full_unstemmed Leishmania Lipophosphoglycan Triggers Caspase-11 and the Non-canonical Activation of the NLRP3 Inflammasome
title_sort Leishmania Lipophosphoglycan Triggers Caspase-11 and the Non-canonical Activation of the NLRP3 Inflammasome
author Carvalho, Renan Villanova Homem de
author_facet Carvalho, Renan Villanova Homem de
Andrade, Warrison A.
Souza Júnior, Djalma Lima
Dilucca, Marisa
Oliveira, Caroline Vitória Fernandes Souza de
Wang, Kun
Nogueira, Paula Monalisa
Rugani, Jeronimo Marteleto Nunes
Soares, Rodrigo Pedro Pinto
Beverley, Stephen M.
Shao, Feng
Zamboni, Dario Simoes
author_role author
author2 Andrade, Warrison A.
Souza Júnior, Djalma Lima
Dilucca, Marisa
Oliveira, Caroline Vitória Fernandes Souza de
Wang, Kun
Nogueira, Paula Monalisa
Rugani, Jeronimo Marteleto Nunes
Soares, Rodrigo Pedro Pinto
Beverley, Stephen M.
Shao, Feng
Zamboni, Dario Simoes
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Renan Villanova Homem de
Andrade, Warrison A.
Souza Júnior, Djalma Lima
Dilucca, Marisa
Oliveira, Caroline Vitória Fernandes Souza de
Wang, Kun
Nogueira, Paula Monalisa
Rugani, Jeronimo Marteleto Nunes
Soares, Rodrigo Pedro Pinto
Beverley, Stephen M.
Shao, Feng
Zamboni, Dario Simoes
dc.subject.en.pt_BR.fl_str_mv Parasites
Leishmania
topic Parasites
Leishmania
description Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeirão Preto, SP, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-10-18T17:51:32Z
dc.date.available.fl_str_mv 2019-10-18T17:51:32Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, Renan Villanova Homem de et al. Leishmania Lipophosphoglycan Triggers Caspase-11 and the Non-canonical Activation of the NLRP3 Inflammasome. Cell Reports, v. 26, n. 2, p. 429-437, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36581
dc.identifier.issn.pt_BR.fl_str_mv 2211-1247
dc.identifier.doi.none.fl_str_mv 10.1016/j.celrep.2018.12.047
identifier_str_mv CARVALHO, Renan Villanova Homem de et al. Leishmania Lipophosphoglycan Triggers Caspase-11 and the Non-canonical Activation of the NLRP3 Inflammasome. Cell Reports, v. 26, n. 2, p. 429-437, 2019.
2211-1247
10.1016/j.celrep.2018.12.047
url https://www.arca.fiocruz.br/handle/icict/36581
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/36581/1/license.txt
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