In vitro and in vivo growth inhibition of human acute promyelocytic leukemia HL-60 cells by Guatteria megalophylla Diels (Annonaceae) leaf essential oil

Detalhes bibliográficos
Autor(a) principal: Costa, Rafaela G. A.
Data de Publicação: 2020
Outros Autores: Anunciação, Talita A. da, Araujo, Morgana de S., Souza, César A., Dias, Rosane Borges, Sales, Caroline Brandi Schlaepfer, Rocha, Clarissa Araújo Gurgel, Soares, Milena Botelho Pereira, Silva, Felipe Moura Araujo da, Koolen, Hector Henrique Ferreira, Costa, Emmanoel Vilaça, Bezerra, Daniel Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/40725
Resumo: Brazilian agencies Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM) and Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).
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spelling Costa, Rafaela G. A.Anunciação, Talita A. daAraujo, Morgana de S.Souza, César A.Dias, Rosane BorgesSales, Caroline Brandi SchlaepferRocha, Clarissa Araújo GurgelSoares, Milena Botelho PereiraSilva, Felipe Moura Araujo daKoolen, Hector Henrique FerreiraCosta, Emmanoel VilaçaBezerra, Daniel Pereira2020-04-13T17:49:25Z2020-04-13T17:49:25Z2020COSTA, Rafaela G. A. et al. In vitro and in vivo growth inhibition of human acute promyelocytic leukemia HL-60 cells by Guatteria megalophylla Diels (Annonaceae) leaf essential oil. Biomedicine and Pharmacotherapy, v. 122, 2020.0753-3322https://www.arca.fiocruz.br/handle/icict/4072510.1016/j.biopha.2019.109713Brazilian agencies Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM) and Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Federal University of Amazonas. Department of Chemistry, Manaus, AM, Brazil.Federal University of Amazonas. Department of Chemistry, Manaus, AM, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Federal University of Bahia. Department of Biomorphology, Institute of Health Sciences. Salvador, Bahia, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Federal University of Amazonas. Department of Chemistry, Manaus, AM, Brazil.Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil.Federal University of Amazonas. Department of Chemistry, Manaus, AM, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Guatteria megalophylla Diels (Annonaceae) is an 8-10 m tall tree that grows near streams and is widely spread throughout Colombian, Ecuadorian, Peruvian, Brazilian and Guianese Amazon rainforest. Herein, we investigated for the first time the chemical composition and in vitro and in vivo anti-leukemia potential of G. megalophylla leaf essential oil (EO) using human promyelocytic leukemia HL-60 cells as model. EO was obtained by a hydrodistillation clevenger-type apparatus and characterized quali- and quantitatively by GC-MS and GC-FID, respectively. In vitro cytotoxic potential of EO was evaluated in human cancer cell lines (HL-60, MCF-7 CAL27, HSC-3, HepG2 and HCT116) and in human non-cancer cell line (MRC-5) by Alamar blue method. Annexin V/propidium iodide staining, cell cycle distribution and reactive oxygen species (ROS) were assessed by flow cytometry for HL-60 cells treated with EO. In vivo efficacy of EO (50 and 100 mg/kg) was evaluated in C.B-17 SCID mice with HL-60 cell xenografts. Chemical composition analyses showed spathulenol, γ-muurolene, bicyclogermacrene, β-elemene and δ-elemene as main constituents of assayed sample. EO displayed in vitro cytotoxicity, including anti-leukemia effect with IC50 value of 12.51 μg/mL for HL-60 cells. EO treatment caused augment of phosphatidylserine externalization and DNA fragmentation without increasing of ROS in HL-60 cells. In vivo tumor mass inhibition rates of EO was 16.6-48.8 %. These data indicate anti-leukemia potential of G. megalophylla leaf EO.engElsevierGuatteria megalophyllaÓleo essencialSpathulenolAnti-leucemiaGuatteria megalophyllaEssential oilSpathulenolAnti-leukemiaIn vitro and in vivo growth inhibition of human acute promyelocytic leukemia HL-60 cells by Guatteria megalophylla Diels (Annonaceae) leaf essential oilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/40725/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCosta RGA In vitro and in vivo growth...pdfCosta RGA In vitro and in vivo growth...pdfapplication/pdf2833220https://www.arca.fiocruz.br/bitstream/icict/40725/2/Costa%20RGA%20In%20vitro%20and%20in%20vivo%20growth...pdff13d245a2c315d69ec50da6076dae9edMD52TEXTCosta RGA In 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dc.title.pt_BR.fl_str_mv In vitro and in vivo growth inhibition of human acute promyelocytic leukemia HL-60 cells by Guatteria megalophylla Diels (Annonaceae) leaf essential oil
title In vitro and in vivo growth inhibition of human acute promyelocytic leukemia HL-60 cells by Guatteria megalophylla Diels (Annonaceae) leaf essential oil
spellingShingle In vitro and in vivo growth inhibition of human acute promyelocytic leukemia HL-60 cells by Guatteria megalophylla Diels (Annonaceae) leaf essential oil
Costa, Rafaela G. A.
Guatteria megalophylla
Óleo essencial
Spathulenol
Anti-leucemia
Guatteria megalophylla
Essential oil
Spathulenol
Anti-leukemia
title_short In vitro and in vivo growth inhibition of human acute promyelocytic leukemia HL-60 cells by Guatteria megalophylla Diels (Annonaceae) leaf essential oil
title_full In vitro and in vivo growth inhibition of human acute promyelocytic leukemia HL-60 cells by Guatteria megalophylla Diels (Annonaceae) leaf essential oil
title_fullStr In vitro and in vivo growth inhibition of human acute promyelocytic leukemia HL-60 cells by Guatteria megalophylla Diels (Annonaceae) leaf essential oil
title_full_unstemmed In vitro and in vivo growth inhibition of human acute promyelocytic leukemia HL-60 cells by Guatteria megalophylla Diels (Annonaceae) leaf essential oil
title_sort In vitro and in vivo growth inhibition of human acute promyelocytic leukemia HL-60 cells by Guatteria megalophylla Diels (Annonaceae) leaf essential oil
author Costa, Rafaela G. A.
author_facet Costa, Rafaela G. A.
Anunciação, Talita A. da
Araujo, Morgana de S.
Souza, César A.
Dias, Rosane Borges
Sales, Caroline Brandi Schlaepfer
Rocha, Clarissa Araújo Gurgel
Soares, Milena Botelho Pereira
Silva, Felipe Moura Araujo da
Koolen, Hector Henrique Ferreira
Costa, Emmanoel Vilaça
Bezerra, Daniel Pereira
author_role author
author2 Anunciação, Talita A. da
Araujo, Morgana de S.
Souza, César A.
Dias, Rosane Borges
Sales, Caroline Brandi Schlaepfer
Rocha, Clarissa Araújo Gurgel
Soares, Milena Botelho Pereira
Silva, Felipe Moura Araujo da
Koolen, Hector Henrique Ferreira
Costa, Emmanoel Vilaça
Bezerra, Daniel Pereira
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Rafaela G. A.
Anunciação, Talita A. da
Araujo, Morgana de S.
Souza, César A.
Dias, Rosane Borges
Sales, Caroline Brandi Schlaepfer
Rocha, Clarissa Araújo Gurgel
Soares, Milena Botelho Pereira
Silva, Felipe Moura Araujo da
Koolen, Hector Henrique Ferreira
Costa, Emmanoel Vilaça
Bezerra, Daniel Pereira
dc.subject.other.pt_BR.fl_str_mv Guatteria megalophylla
Óleo essencial
Spathulenol
Anti-leucemia
topic Guatteria megalophylla
Óleo essencial
Spathulenol
Anti-leucemia
Guatteria megalophylla
Essential oil
Spathulenol
Anti-leukemia
dc.subject.en.pt_BR.fl_str_mv Guatteria megalophylla
Essential oil
Spathulenol
Anti-leukemia
description Brazilian agencies Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM) and Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-04-13T17:49:25Z
dc.date.available.fl_str_mv 2020-04-13T17:49:25Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv COSTA, Rafaela G. A. et al. In vitro and in vivo growth inhibition of human acute promyelocytic leukemia HL-60 cells by Guatteria megalophylla Diels (Annonaceae) leaf essential oil. Biomedicine and Pharmacotherapy, v. 122, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/40725
dc.identifier.issn.pt_BR.fl_str_mv 0753-3322
dc.identifier.doi.none.fl_str_mv 10.1016/j.biopha.2019.109713
identifier_str_mv COSTA, Rafaela G. A. et al. In vitro and in vivo growth inhibition of human acute promyelocytic leukemia HL-60 cells by Guatteria megalophylla Diels (Annonaceae) leaf essential oil. Biomedicine and Pharmacotherapy, v. 122, 2020.
0753-3322
10.1016/j.biopha.2019.109713
url https://www.arca.fiocruz.br/handle/icict/40725
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dc.publisher.none.fl_str_mv Elsevier
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