Semaphorin-3A-Related Reduction of Thymocyte Migration in Chemically Induced Diabetic Mice

Detalhes bibliográficos
Autor(a) principal: Francelin, Carolina
Data de Publicação: 2020
Outros Autores: Geniseli, Ieda, Nagib, Patrícia, Gameiro, Jacy, Savino, Wilson, Verinaud, Liana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/45546
Resumo: Universidade de Campinas. Instituto de Biologia. Departamento de Genética, Microbiologia e Imunologia. Laboratório de Pesquisa Autoimune. Campinas, SP, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Imunomodulação. Rio de Janeiro, RJ, Brasil / University of Alabama at Birmingham. Department of Ophthalmology,. Birmingham, AL, USA.
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spelling Francelin, CarolinaGeniseli, IedaNagib, PatríciaGameiro, JacySavino, WilsonVerinaud, Liana2021-01-09T19:00:27Z2021-01-09T19:00:27Z2020FRANCELIN, Carolina et al. Semaphorin-3A-Related Reduction of Thymocyte Migration in Chemically Induced Diabetic Mice. NeuroImmunomodulation, v. 27, p. 28-37, Mar. 2020.1021-7401https://www.arca.fiocruz.br/handle/icict/4554610.1159/000506054engKarger PublishersTimoMigração de timócitosAtrofiaDiabetesSemaforinaThymusSemaphorinAtrophyDiabetesThymocyte migrationSemaphorin-3A-Related Reduction of Thymocyte Migration in Chemically Induced Diabetic Miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de Campinas. Instituto de Biologia. Departamento de Genética, Microbiologia e Imunologia. Laboratório de Pesquisa Autoimune. Campinas, SP, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Imunomodulação. Rio de Janeiro, RJ, Brasil / University of Alabama at Birmingham. Department of Ophthalmology,. Birmingham, AL, USA.Universidade de Campinas. Instituto de Biologia. Departamento de Biologia Funcional e Estrutural. Campinas, SP,. Brasil.Universidade Federal de Goiânia. Instituto de Tropical de Patologia e Saúde Pública. Goiânia, GO, Brasil.Universidade Federal de Juiz de Fora. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Imunomodulação. Rio de Janeiro, RJ, Brasil.Universidade de Campinas. Instituto de Biologia. Departamento de Biologia Funcional e Estrutural. Campinas, SP,. Brasil.Previous work revealed the existence of a severe thymic atrophy with massive loss of immature CD4+CD8+ thymocytes in animals developing insulin-dependent diabetes, chemically induced by alloxan. Furthermore, the intrathymic expression of chemokines, such as CXCL12, is changed in these animals, suggesting that cell migrationrelated patterns may be altered. One molecular interaction involved in normal thymocyte migration is that mediated by soluble semaphorin-3A and its cognate receptor neuropilin- 1. Objectives: We investigated herein the expression and role of semaphorin-3A in the migratory responses of thymocytes from alloxan-induced diabetic mice. We characterized semaphorin-3A and its receptor, neuropilin-1, in thymuses from control and diabetic mice as well as semaphorin-3Adependent migration of developing thymocytes in both control and diabetic animals. Methods: Diabetes was chemically induced after a single injection of alloxan in young adult BALB/c mice. Thymocytes were excised from control and diabetic individuals and subjected to cytofluorometry for simultaneous detection of semaphorin-3A or neuropilin- 1 in CD4/CD8-defined subsets. Cell migration in response to semaphorin-3A was performed using cell migration transwell chambers. Results: Confirming previous data, we observed a severe decrease in the total numbers of thymocytes in diabetic mice, which comprised alterations in both immature (double-negative subpopulations) and mature CD4/ CD8-defined thymocyte subsets. These were accompanied by a decrease in the absolute numbers of semaphorin-3Abearing thymocytes, comprising CD4–CD8–, CD4+CD8+, and CD4–CD8+ cells. Additionally, immature CD4–CD8– and CD4+CD8+ developing T cells exhibited a decrease in the membrane density of semaphorin-3A. The relative and absolute numbers of neuropilin-1-positive thymocytes were also decreased in diabetic mouse thymocytes compared to controls, as seen in CD4–CD8–, CD4+CD8+, and CD4–CD8+ cell subpopulations. Functionally, we observed a decrease in the SEMA-3A: Reduction of Thymocyte Migration in Chemically Induced Diabetic Mice Neuroimmunomodulation 2020;27:28–37 29 DOI: 10.1159/000506054 chemorepulsive role of semaphorin-3A, as revealed by transwell migration chambers. Such an effect was seen in all immature and mature thymocyte subsets..info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45546/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALWilsonSavino_etal_IOC_2020.pdfWilsonSavino_etal_IOC_2020.pdfapplication/pdf572720https://www.arca.fiocruz.br/bitstream/icict/45546/2/WilsonSavino_etal_IOC_2020.pdf61fd95362eee356825143b6804251c7aMD52TEXTWilsonSavino_etal_IOC_2020.pdf.txtWilsonSavino_etal_IOC_2020.pdf.txtExtracted texttext/plain36994https://www.arca.fiocruz.br/bitstream/icict/45546/3/WilsonSavino_etal_IOC_2020.pdf.txt4a89b0e1f91a87e3a2e246b32cfe0e3dMD53icict/455462021-01-10 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dc.title.pt_BR.fl_str_mv Semaphorin-3A-Related Reduction of Thymocyte Migration in Chemically Induced Diabetic Mice
title Semaphorin-3A-Related Reduction of Thymocyte Migration in Chemically Induced Diabetic Mice
spellingShingle Semaphorin-3A-Related Reduction of Thymocyte Migration in Chemically Induced Diabetic Mice
Francelin, Carolina
Timo
Migração de timócitos
Atrofia
Diabetes
Semaforina
Thymus
Semaphorin
Atrophy
Diabetes
Thymocyte migration
title_short Semaphorin-3A-Related Reduction of Thymocyte Migration in Chemically Induced Diabetic Mice
title_full Semaphorin-3A-Related Reduction of Thymocyte Migration in Chemically Induced Diabetic Mice
title_fullStr Semaphorin-3A-Related Reduction of Thymocyte Migration in Chemically Induced Diabetic Mice
title_full_unstemmed Semaphorin-3A-Related Reduction of Thymocyte Migration in Chemically Induced Diabetic Mice
title_sort Semaphorin-3A-Related Reduction of Thymocyte Migration in Chemically Induced Diabetic Mice
author Francelin, Carolina
author_facet Francelin, Carolina
Geniseli, Ieda
Nagib, Patrícia
Gameiro, Jacy
Savino, Wilson
Verinaud, Liana
author_role author
author2 Geniseli, Ieda
Nagib, Patrícia
Gameiro, Jacy
Savino, Wilson
Verinaud, Liana
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Francelin, Carolina
Geniseli, Ieda
Nagib, Patrícia
Gameiro, Jacy
Savino, Wilson
Verinaud, Liana
dc.subject.other.pt_BR.fl_str_mv Timo
Migração de timócitos
Atrofia
Diabetes
Semaforina
topic Timo
Migração de timócitos
Atrofia
Diabetes
Semaforina
Thymus
Semaphorin
Atrophy
Diabetes
Thymocyte migration
dc.subject.en.pt_BR.fl_str_mv Thymus
Semaphorin
Atrophy
Diabetes
Thymocyte migration
description Universidade de Campinas. Instituto de Biologia. Departamento de Genética, Microbiologia e Imunologia. Laboratório de Pesquisa Autoimune. Campinas, SP, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Imunomodulação. Rio de Janeiro, RJ, Brasil / University of Alabama at Birmingham. Department of Ophthalmology,. Birmingham, AL, USA.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-01-09T19:00:27Z
dc.date.available.fl_str_mv 2021-01-09T19:00:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FRANCELIN, Carolina et al. Semaphorin-3A-Related Reduction of Thymocyte Migration in Chemically Induced Diabetic Mice. NeuroImmunomodulation, v. 27, p. 28-37, Mar. 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/45546
dc.identifier.issn.pt_BR.fl_str_mv 1021-7401
dc.identifier.doi.none.fl_str_mv 10.1159/000506054
identifier_str_mv FRANCELIN, Carolina et al. Semaphorin-3A-Related Reduction of Thymocyte Migration in Chemically Induced Diabetic Mice. NeuroImmunomodulation, v. 27, p. 28-37, Mar. 2020.
1021-7401
10.1159/000506054
url https://www.arca.fiocruz.br/handle/icict/45546
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Karger Publishers
publisher.none.fl_str_mv Karger Publishers
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/45546/2/WilsonSavino_etal_IOC_2020.pdf
https://www.arca.fiocruz.br/bitstream/icict/45546/3/WilsonSavino_etal_IOC_2020.pdf.txt
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