Chitotriosidase 1 in the cerebrospinal fluid as a putative biomarker for HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) progression
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/55686 |
Resumo: | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. |
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Gomes, Yago Côrtes PinheiroFreitas, Nicole LardiniSouza, Flávia SantosSandim, VanessaPereira, Denise AbreuNogueira, Fábio César SousaEchevarria-Lima, JulianaLeite, Ana Claudia Celestino BezerraLima, Marco Antonio Sales DantasSilva, Marcus Tulius TeixeiraAraújo, Abelardo Queiroz CamposVicente, Ana Carolina PauloEspíndola, Otávio Melo2022-11-17T18:24:57Z2022-11-17T18:24:57Z2022GOMES, Yago Côrtes Pinheiro et al. Chitotriosidase 1 in the cerebrospinal fluid as a putative biomarker for HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) progression. Frontiers in Immunology, v. 13, p. 1-13, 2022.1664-3224https://www.arca.fiocruz.br/handle/icict/5568610.3389/fimmu.2022.949516engFrontiers MediaChitotriosidase 1 in the cerebrospinal fluid as a putative biomarker for HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) progressioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Instituto Nacional do Câncer. Programa de Oncobiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química. Laboratório de Apoio ao Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Química. Unidade de Proteômica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Human T-lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is an inflammatory neurodegenerative disease that affects motor, urinary, intestinal, and sensory functions. Typically, HAM/TSP is slowly progressive, but it may vary from limited motor disability after decades (very slow progression) to loss of motor function in a few years from disease onset (rapid). In this study, we aimed to identify prognostic biomarkers for HAM/TSP to support patient management. Thus, proteomic analysis of the cerebrospinal fluid (CSF) was performed with samples from HTLV-1 asymptomatic carriers (AC) (n=13) and HAM/TSP patients (n=21) with rapid, typical, and very slow progression using quantitative label-free liquid chromatography/tandem mass spectrometry. Enrichment analyses were also carried out to identify key biological processes associated with distinct neurological conditions in HTLV-1 infection. Candidate biomarkers were validated by ELISA in paired CSF and serum samples, and samples from HTLV-1-seronegative individuals (n=9) were used as controls. CSF analysis identified 602 proteins. Leukocyte/cell activation, immune response processes and neurodegeneration pathways were enriched in rapid progressors. Conversely, HTLV-1 AC and HAM/TSP patients with typical and very slow progression had enriched processes for nervous system development. Differential expression analysis showed that soluble vascular cell adhesion molecule 1 (sVCAM-1), chitotriosidase 1 (CHIT1), and cathepsin C (CTSC) were upregulated in HAM/TSP. However, only CHIT1 was significantly elevated after validation, particularly in HAM/TSP rapid progressors. In contrast, none of these biomarkers were altered in serum. Additionally, CSF CHIT1 levels in HAM/TSP patients positively correlated with the speed of HAM/TSP progression, defined as points in the IPEC-2 HAM/TSP disability scale per year of disease, and with CSF levels of phosphorylated neurofilament heavy chain, neopterin, CXCL5, CXCL10, and CXCL11. In conclusion, higher CSF levels of CHIT1 were associated with HAM/TSP rapid progression and correlated with other biomarkers of neuroinflammation and neurodegeneration. Therefore, we propose CHIT1 as an additional or alternative CSF biomarker to identify HAM/TSP patients with a worse prognosis.HAM/TSPHTLV-1BiomarkersCerebrospinal fluidChitotriosidase 1NeurodegenerationProteomic analysisSoluble VCAM-1info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/55686/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALChitotriosidase_Yago_Gomes_etal_INI_2022.pdfChitotriosidase_Yago_Gomes_etal_INI_2022.pdfapplication/pdf3359617https://www.arca.fiocruz.br/bitstream/icict/55686/2/Chitotriosidase_Yago_Gomes_etal_INI_2022.pdfdffc30a752ae5c0e278937d975656dccMD52icict/556862023-09-14 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| dc.title.en_US.fl_str_mv |
Chitotriosidase 1 in the cerebrospinal fluid as a putative biomarker for HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) progression |
| title |
Chitotriosidase 1 in the cerebrospinal fluid as a putative biomarker for HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) progression |
| spellingShingle |
Chitotriosidase 1 in the cerebrospinal fluid as a putative biomarker for HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) progression Gomes, Yago Côrtes Pinheiro HAM/TSP HTLV-1 Biomarkers Cerebrospinal fluid Chitotriosidase 1 Neurodegeneration Proteomic analysis Soluble VCAM-1 |
| title_short |
Chitotriosidase 1 in the cerebrospinal fluid as a putative biomarker for HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) progression |
| title_full |
Chitotriosidase 1 in the cerebrospinal fluid as a putative biomarker for HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) progression |
| title_fullStr |
Chitotriosidase 1 in the cerebrospinal fluid as a putative biomarker for HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) progression |
| title_full_unstemmed |
Chitotriosidase 1 in the cerebrospinal fluid as a putative biomarker for HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) progression |
| title_sort |
Chitotriosidase 1 in the cerebrospinal fluid as a putative biomarker for HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) progression |
| author |
Gomes, Yago Côrtes Pinheiro |
| author_facet |
Gomes, Yago Côrtes Pinheiro Freitas, Nicole Lardini Souza, Flávia Santos Sandim, Vanessa Pereira, Denise Abreu Nogueira, Fábio César Sousa Echevarria-Lima, Juliana Leite, Ana Claudia Celestino Bezerra Lima, Marco Antonio Sales Dantas Silva, Marcus Tulius Teixeira Araújo, Abelardo Queiroz Campos Vicente, Ana Carolina Paulo Espíndola, Otávio Melo |
| author_role |
author |
| author2 |
Freitas, Nicole Lardini Souza, Flávia Santos Sandim, Vanessa Pereira, Denise Abreu Nogueira, Fábio César Sousa Echevarria-Lima, Juliana Leite, Ana Claudia Celestino Bezerra Lima, Marco Antonio Sales Dantas Silva, Marcus Tulius Teixeira Araújo, Abelardo Queiroz Campos Vicente, Ana Carolina Paulo Espíndola, Otávio Melo |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Gomes, Yago Côrtes Pinheiro Freitas, Nicole Lardini Souza, Flávia Santos Sandim, Vanessa Pereira, Denise Abreu Nogueira, Fábio César Sousa Echevarria-Lima, Juliana Leite, Ana Claudia Celestino Bezerra Lima, Marco Antonio Sales Dantas Silva, Marcus Tulius Teixeira Araújo, Abelardo Queiroz Campos Vicente, Ana Carolina Paulo Espíndola, Otávio Melo |
| dc.subject.en.en_US.fl_str_mv |
HAM/TSP HTLV-1 Biomarkers Cerebrospinal fluid Chitotriosidase 1 Neurodegeneration Proteomic analysis Soluble VCAM-1 |
| topic |
HAM/TSP HTLV-1 Biomarkers Cerebrospinal fluid Chitotriosidase 1 Neurodegeneration Proteomic analysis Soluble VCAM-1 |
| description |
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. |
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2022 |
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2022-11-17T18:24:57Z |
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2022-11-17T18:24:57Z |
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2022 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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GOMES, Yago Côrtes Pinheiro et al. Chitotriosidase 1 in the cerebrospinal fluid as a putative biomarker for HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) progression. Frontiers in Immunology, v. 13, p. 1-13, 2022. |
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https://www.arca.fiocruz.br/handle/icict/55686 |
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1664-3224 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fimmu.2022.949516 |
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GOMES, Yago Côrtes Pinheiro et al. Chitotriosidase 1 in the cerebrospinal fluid as a putative biomarker for HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) progression. Frontiers in Immunology, v. 13, p. 1-13, 2022. 1664-3224 10.3389/fimmu.2022.949516 |
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