Hydroxyurea alters circulating monocyte subsets and dampens its inflammatory potential in sickle cell anemia patients
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/37051 |
Resumo: | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil. |
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Guarda, Caroline Conceição daMattos, Paulo Sérgio de Morais da SilveiraYahouédéhou, Sètondji C. M. A.Santiago, Rayra PereiraAleluia, Milena MagalhãesFigueiredo, Camylla Villas BoasFiuza, Luciana MagalhaesCarvalho, Suellen PinheiroOliveira, Rodrigo Mota deNascimento, Valma Maria LopesLuz, Nívea FariasBorges, Valéria de MatosAndrade, Bruno de BezerrilGonçalves, Marilda de Souza2019-11-14T12:49:49Z2019-11-14T12:49:49Z2019GUARDA, Caroline Conceição da et al. Hydroxyurea alters circulating monocyte subsets and dampens its inflammatory potential in sickle cell anemia patients. Scientific Reports, v. 9, p. 1-11, 2019.2045-2322https://www.arca.fiocruz.br/handle/icict/3705110.1038/s41598-019-51339-xengNature ResearchAnemia falciformeHidroxiureiaTratamentoHumanosAnemia, Sickle CellHydroxyureaTreatmentHumansHydroxyurea alters circulating monocyte subsets and dampens its inflammatory potential in sickle cell anemia patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil / Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative. Salvador, BA, Brazil / Faculdade de Tecnologia e Ciências. Curso de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil.Fundação de Hematologia e Hemoterapia do Estado da Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil / Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative. Salvador, BA, Brazil / Faculdade de Tecnologia e Ciências. Curso de Medicina. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil / Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative. Salvador, BA, Brazil / Faculdade de Tecnologia e Ciências. Curso de Medicina. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil / Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative. Salvador, BA, Brazil / Faculdade de Tecnologia e Ciências. Curso de Medicina. Salvador, BA, Brasil / Universidade Salvador. Laureate Universities. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil.Sickle cell anemia (SCA) is a hemolytic disease in which vaso-occlusion is an important pathophysiological mechanism. The treatment is based on hydroxyurea (HU), which decreases leukocyte counts and increases fetal hemoglobin synthesis. Different cell types are thought to contribute to vaso-occlusion. Nevertheless, the role of monocytes subsets remains unclear. We investigated frequencies of monocytes subsets in blood and their response to HU therapy, testing their ability to express pro-inflammatory molecules and tissue factor (TF). We identified major changes in monocyte subsets, with classical monocytes (CD14++CD16-) appearing highly frequent in who were not taking HU, whereas those with patrolling phenotype (CD14dimCD16+) were enriched in individuals undergoing therapy. Additionally, HU decreased the production of TNF-α, IL1-β, IL-6, IL-8 as well as TF by the LPS-activated monocytes. Likewise, frequency of TF-expressing monocytes is increased in patients with previous vaso-occlusion. Moreover, activated monocytes expressing TF produced several pro-inflammatory cytokines simultaneously. Such polyfunctional capacity was dramatically dampened by HU therapy. The frequency of classical monocytes subset was positively correlated with percentage cytokine producing cells upon LPS stimulation. These findings suggest that classical monocytes are the subset responsible for multiple pro-inflammatory cytokine production and possibly drive inflammation and vaso-occlusion in SCA which is damped by HU.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/37051/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGuarda, C.C. Hydroxyuera....pdfGuarda, C.C. Hydroxyuera....pdfapplication/pdf3656637https://www.arca.fiocruz.br/bitstream/icict/37051/2/Guarda%2c%20C.C.%20Hydroxyuera....pdf14aab9fd62e1f2949b605d6f5956c6e4MD52TEXTGuarda, C.C. Hydroxyuera....pdf.txtGuarda, C.C. 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| dc.title.pt_BR.fl_str_mv |
Hydroxyurea alters circulating monocyte subsets and dampens its inflammatory potential in sickle cell anemia patients |
| title |
Hydroxyurea alters circulating monocyte subsets and dampens its inflammatory potential in sickle cell anemia patients |
| spellingShingle |
Hydroxyurea alters circulating monocyte subsets and dampens its inflammatory potential in sickle cell anemia patients Guarda, Caroline Conceição da Anemia falciforme Hidroxiureia Tratamento Humanos Anemia, Sickle Cell Hydroxyurea Treatment Humans |
| title_short |
Hydroxyurea alters circulating monocyte subsets and dampens its inflammatory potential in sickle cell anemia patients |
| title_full |
Hydroxyurea alters circulating monocyte subsets and dampens its inflammatory potential in sickle cell anemia patients |
| title_fullStr |
Hydroxyurea alters circulating monocyte subsets and dampens its inflammatory potential in sickle cell anemia patients |
| title_full_unstemmed |
Hydroxyurea alters circulating monocyte subsets and dampens its inflammatory potential in sickle cell anemia patients |
| title_sort |
Hydroxyurea alters circulating monocyte subsets and dampens its inflammatory potential in sickle cell anemia patients |
| author |
Guarda, Caroline Conceição da |
| author_facet |
Guarda, Caroline Conceição da Mattos, Paulo Sérgio de Morais da Silveira Yahouédéhou, Sètondji C. M. A. Santiago, Rayra Pereira Aleluia, Milena Magalhães Figueiredo, Camylla Villas Boas Fiuza, Luciana Magalhaes Carvalho, Suellen Pinheiro Oliveira, Rodrigo Mota de Nascimento, Valma Maria Lopes Luz, Nívea Farias Borges, Valéria de Matos Andrade, Bruno de Bezerril Gonçalves, Marilda de Souza |
| author_role |
author |
| author2 |
Mattos, Paulo Sérgio de Morais da Silveira Yahouédéhou, Sètondji C. M. A. Santiago, Rayra Pereira Aleluia, Milena Magalhães Figueiredo, Camylla Villas Boas Fiuza, Luciana Magalhaes Carvalho, Suellen Pinheiro Oliveira, Rodrigo Mota de Nascimento, Valma Maria Lopes Luz, Nívea Farias Borges, Valéria de Matos Andrade, Bruno de Bezerril Gonçalves, Marilda de Souza |
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author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Guarda, Caroline Conceição da Mattos, Paulo Sérgio de Morais da Silveira Yahouédéhou, Sètondji C. M. A. Santiago, Rayra Pereira Aleluia, Milena Magalhães Figueiredo, Camylla Villas Boas Fiuza, Luciana Magalhaes Carvalho, Suellen Pinheiro Oliveira, Rodrigo Mota de Nascimento, Valma Maria Lopes Luz, Nívea Farias Borges, Valéria de Matos Andrade, Bruno de Bezerril Gonçalves, Marilda de Souza |
| dc.subject.other.pt_BR.fl_str_mv |
Anemia falciforme Hidroxiureia Tratamento Humanos |
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Anemia falciforme Hidroxiureia Tratamento Humanos Anemia, Sickle Cell Hydroxyurea Treatment Humans |
| dc.subject.en.pt_BR.fl_str_mv |
Anemia, Sickle Cell Hydroxyurea Treatment Humans |
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Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil. |
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2019 |
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2019-11-14T12:49:49Z |
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2019-11-14T12:49:49Z |
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2019 |
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info:eu-repo/semantics/article |
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GUARDA, Caroline Conceição da et al. Hydroxyurea alters circulating monocyte subsets and dampens its inflammatory potential in sickle cell anemia patients. Scientific Reports, v. 9, p. 1-11, 2019. |
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https://www.arca.fiocruz.br/handle/icict/37051 |
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2045-2322 |
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10.1038/s41598-019-51339-x |
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GUARDA, Caroline Conceição da et al. Hydroxyurea alters circulating monocyte subsets and dampens its inflammatory potential in sickle cell anemia patients. Scientific Reports, v. 9, p. 1-11, 2019. 2045-2322 10.1038/s41598-019-51339-x |
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