Metabolic switches during the first steps of adipogenic Stem Cells differentiation

Detalhes bibliográficos
Autor(a) principal: Drehmer, Daiana Leila
Data de Publicação: 2016
Outros Autores: Aguiar, Alessandra Melo de, Brandt, Anna Paula, Petiz, Lyvia, Cadena, Sílvia Maria Suter Correia, Rebelatto, Carmem K., Brofman, Paulo Roberto Slud, Filipak Neto, Francisco, Dallagiovanna, Bruno, Abud, Ana Paula Ressetti
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20405
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
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spelling Drehmer, Daiana LeilaAguiar, Alessandra Melo deBrandt, Anna PaulaPetiz, LyviaCadena, Sílvia Maria Suter CorreiaRebelatto, Carmem K.Brofman, Paulo Roberto SludFilipak Neto, FranciscoDallagiovanna, BrunoAbud, Ana Paula Ressetti2017-07-25T18:48:05Z2017-07-25T18:48:05Z2016DREHMER, Daiana Leila et al. Metabolic switches during the first steps of adipogenic stem cells differentiation. Stem Cell Research. v. 17, n. 2, p. 413-421, Sept. 2016.1876-7753https://www.arca.fiocruz.br/handle/icict/2040510.1016/j.scr.2016.09.001porElsevierCitoproteçãoAdult Stem CellsAdipogenesisMetabolismReactive Oxygen SpeciesAntioxidantsCytoprotectionCélulas Madre AdultasAdipogénesisMetabolismoEspecies Reactivas de OxígenoAntioxidantesCitoprotecciónCélulas-Tronco AdultasAdipogeniaMetabolismoEspécies Reativas de OxigênioAntioxidantesMetabolic switches during the first steps of adipogenic Stem Cells differentiationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Universidade Federal do Paraná. Paraná, PR, Brasil.Universidade Federal do Paraná. Paraná, PR, Brasil.Universidade Federal do Paraná. Paraná, PR, Brasil.Pontifícia Universidade Católica do Paraná. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Curitiba, PR, Brasil.Universidade Federal do Paraná. Paraná, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.The understanding of metabolism during cell proliferation and commitment provides a greater insight into the basic biology of cells, allowing future applications. Here we evaluated the energy and oxidative changes during the early adipogenic differentiation of human adipose tissue-derived stromal cells (hASCs). hASCs were maintained under differentiation conditions during 3 and 7 days. Oxygen consumption, mitochondrial mass and membrane potential, reactive oxygen species (ROS) generation, superoxide dismutase (SOD) and catalase activities, non-protein thiols (NPT) concentration and lipid peroxidation were analyzed. We observed that 7 days of adipogenic induction are required to stimulate cells to consume more oxygen and increase mitochondrial activity, indicating organelle maturation and a transition from glycolytic to oxidative energy metabolism. ROS production was only increased after 3 days and may be involved in the differentiation commitment. ROS source was not only the mitochondria and we suggest that NOX proteins are related to ROS generation and therefore adipogenic commitment. ROS production did not change after 7 days, but an increased activity of catalase and NPT concentration as well as a decreased lipid peroxidation were observed. Thus, a short period of differentiation induction is able to change the energetic and oxidative metabolic profile of hASCs and stimulate cytoprotection processes.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20405/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMetabolic switches during the first steps of adipogenic stem cells differentiation.pdfMetabolic switches during the first steps of adipogenic stem cells differentiation.pdfapplication/pdf1451530https://www.arca.fiocruz.br/bitstream/icict/20405/2/Metabolic%20switches%20during%20the%20first%20steps%20of%20adipogenic%20stem%20cells%20differentiation.pdf697c2973a71caa62c71ad654f92cc2eeMD52TEXTMetabolic switches during the first steps of adipogenic stem cells differentiation.pdf.txtMetabolic switches during the first steps of 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dc.title.pt_BR.fl_str_mv Metabolic switches during the first steps of adipogenic Stem Cells differentiation
title Metabolic switches during the first steps of adipogenic Stem Cells differentiation
spellingShingle Metabolic switches during the first steps of adipogenic Stem Cells differentiation
Drehmer, Daiana Leila
Citoproteção
Adult Stem Cells
Adipogenesis
Metabolism
Reactive Oxygen Species
Antioxidants
Cytoprotection
Células Madre Adultas
Adipogénesis
Metabolismo
Especies Reactivas de Oxígeno
Antioxidantes
Citoprotección
Células-Tronco Adultas
Adipogenia
Metabolismo
Espécies Reativas de Oxigênio
Antioxidantes
title_short Metabolic switches during the first steps of adipogenic Stem Cells differentiation
title_full Metabolic switches during the first steps of adipogenic Stem Cells differentiation
title_fullStr Metabolic switches during the first steps of adipogenic Stem Cells differentiation
title_full_unstemmed Metabolic switches during the first steps of adipogenic Stem Cells differentiation
title_sort Metabolic switches during the first steps of adipogenic Stem Cells differentiation
author Drehmer, Daiana Leila
author_facet Drehmer, Daiana Leila
Aguiar, Alessandra Melo de
Brandt, Anna Paula
Petiz, Lyvia
Cadena, Sílvia Maria Suter Correia
Rebelatto, Carmem K.
Brofman, Paulo Roberto Slud
Filipak Neto, Francisco
Dallagiovanna, Bruno
Abud, Ana Paula Ressetti
author_role author
author2 Aguiar, Alessandra Melo de
Brandt, Anna Paula
Petiz, Lyvia
Cadena, Sílvia Maria Suter Correia
Rebelatto, Carmem K.
Brofman, Paulo Roberto Slud
Filipak Neto, Francisco
Dallagiovanna, Bruno
Abud, Ana Paula Ressetti
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Drehmer, Daiana Leila
Aguiar, Alessandra Melo de
Brandt, Anna Paula
Petiz, Lyvia
Cadena, Sílvia Maria Suter Correia
Rebelatto, Carmem K.
Brofman, Paulo Roberto Slud
Filipak Neto, Francisco
Dallagiovanna, Bruno
Abud, Ana Paula Ressetti
dc.subject.other.pt_BR.fl_str_mv Citoproteção
topic Citoproteção
Adult Stem Cells
Adipogenesis
Metabolism
Reactive Oxygen Species
Antioxidants
Cytoprotection
Células Madre Adultas
Adipogénesis
Metabolismo
Especies Reactivas de Oxígeno
Antioxidantes
Citoprotección
Células-Tronco Adultas
Adipogenia
Metabolismo
Espécies Reativas de Oxigênio
Antioxidantes
dc.subject.en.pt_BR.fl_str_mv Adult Stem Cells
Adipogenesis
Metabolism
Reactive Oxygen Species
Antioxidants
Cytoprotection
dc.subject.es.pt_BR.fl_str_mv Células Madre Adultas
Adipogénesis
Metabolismo
Especies Reactivas de Oxígeno
Antioxidantes
Citoprotección
dc.subject.decs.pt_BR.fl_str_mv Células-Tronco Adultas
Adipogenia
Metabolismo
Espécies Reativas de Oxigênio
Antioxidantes
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-07-25T18:48:05Z
dc.date.available.fl_str_mv 2017-07-25T18:48:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DREHMER, Daiana Leila et al. Metabolic switches during the first steps of adipogenic stem cells differentiation. Stem Cell Research. v. 17, n. 2, p. 413-421, Sept. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20405
dc.identifier.issn.pt_BR.fl_str_mv 1876-7753
dc.identifier.doi.pt_BR.fl_str_mv 10.1016/j.scr.2016.09.001
identifier_str_mv DREHMER, Daiana Leila et al. Metabolic switches during the first steps of adipogenic stem cells differentiation. Stem Cell Research. v. 17, n. 2, p. 413-421, Sept. 2016.
1876-7753
10.1016/j.scr.2016.09.001
url https://www.arca.fiocruz.br/handle/icict/20405
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/20405/1/license.txt
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