Solid cation exchange phase to remove interfering anthocyanins in the analysis of other bioactive phenols in red wine.

Detalhes bibliográficos
Autor(a) principal: SILVA, L. F. da
Data de Publicação: 2017
Outros Autores: GUERRA, C. C., KLEIN, D., BERGOLD, A. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1062946
http://dx.doi.org/10.1016/j.foodchem.2017.01.087
Resumo: Bioactive phenols (BPs) are often targets in red wine analysis. However, other compounds interfere in the liquid chromatography methods used for this analysis. Here, purification procedures were tested to eliminate anthocyanin interference during the determination of 19 red-wine BPs. Liquid chromatography, coupled to a diode array detector (HPLC-DAD) and a mass spectrometer (UPLC-MS), was used to compare the direct injection of the samples with solid-phase extractions: reversed-phase (C18) and strong cation-exchange (SCX). The HPLC-DAD method revealed that, out of 13 BPs, only six are selectively analyzed with or without C18 treatment, whereas SCX enabled the detection of all BPs. The recovery with SCX was above 86.6% for eight BPs. Moreover, UPLC-MS demonstrated the potential of SCX sample preparation for the determination of 19 BPs. The developed procedure may be extended to the analysis of other red wine molecules or to other analytical methods where anthocyanins may interfere. Chemical compounds studied in this article trans-Resveratrol (PubChemCID: 445154); trans-?-Viniferin (PubChemCID: 5315233); Quercetin (PubChemCID: 5280343); Myricetin (PubChemCID: 5281672); Kaempferol (PubChemCID: 5280863); trans-Cinnamic acid (PubChemCID: 444539); trans-Piceid (PubChemCID: 5281718); Taxifolin (PubChemCID: 439533) Keywords Bioactive phenols; Red wine; Liquid chromatography; Mass spectrometry; Diode array detector; Sample purification
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spelling Solid cation exchange phase to remove interfering anthocyanins in the analysis of other bioactive phenols in red wine.Sample purificationBioactive phenolsDiode array detectorMass spectometryRed wineLiquid ChromatographyBioactive phenols (BPs) are often targets in red wine analysis. However, other compounds interfere in the liquid chromatography methods used for this analysis. Here, purification procedures were tested to eliminate anthocyanin interference during the determination of 19 red-wine BPs. Liquid chromatography, coupled to a diode array detector (HPLC-DAD) and a mass spectrometer (UPLC-MS), was used to compare the direct injection of the samples with solid-phase extractions: reversed-phase (C18) and strong cation-exchange (SCX). The HPLC-DAD method revealed that, out of 13 BPs, only six are selectively analyzed with or without C18 treatment, whereas SCX enabled the detection of all BPs. The recovery with SCX was above 86.6% for eight BPs. Moreover, UPLC-MS demonstrated the potential of SCX sample preparation for the determination of 19 BPs. The developed procedure may be extended to the analysis of other red wine molecules or to other analytical methods where anthocyanins may interfere. Chemical compounds studied in this article trans-Resveratrol (PubChemCID: 445154); trans-?-Viniferin (PubChemCID: 5315233); Quercetin (PubChemCID: 5280343); Myricetin (PubChemCID: 5281672); Kaempferol (PubChemCID: 5280863); trans-Cinnamic acid (PubChemCID: 444539); trans-Piceid (PubChemCID: 5281718); Taxifolin (PubChemCID: 439533) Keywords Bioactive phenols; Red wine; Liquid chromatography; Mass spectrometry; Diode array detector; Sample purificationLETICIA FLORES DA SILVA, CNPUV; CELITO CRIVELLARO GUERRA, CNPUV; Diandra Klein, Faculdade de Farmácia, Universidade de Caxias do Sul; Ana Maria Bergold, Laboratório de Química Farmacêutica, Universidade Federal do Rio Grande do Sul.SILVA, L. F. daGUERRA, C. C.KLEIN, D.BERGOLD, A. M.2017-02-07T11:11:11Z2017-02-07T11:11:11Z2017-02-0720172019-05-06T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFood Chemistryl, v. 227, p. 158-165, 2017.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1062946http://dx.doi.org/10.1016/j.foodchem.2017.01.087enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T04:09:00Zoai:www.alice.cnptia.embrapa.br:doc/1062946Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T04:09falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T04:09Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Solid cation exchange phase to remove interfering anthocyanins in the analysis of other bioactive phenols in red wine.
title Solid cation exchange phase to remove interfering anthocyanins in the analysis of other bioactive phenols in red wine.
spellingShingle Solid cation exchange phase to remove interfering anthocyanins in the analysis of other bioactive phenols in red wine.
SILVA, L. F. da
Sample purification
Bioactive phenols
Diode array detector
Mass spectometry
Red wine
Liquid Chromatography
title_short Solid cation exchange phase to remove interfering anthocyanins in the analysis of other bioactive phenols in red wine.
title_full Solid cation exchange phase to remove interfering anthocyanins in the analysis of other bioactive phenols in red wine.
title_fullStr Solid cation exchange phase to remove interfering anthocyanins in the analysis of other bioactive phenols in red wine.
title_full_unstemmed Solid cation exchange phase to remove interfering anthocyanins in the analysis of other bioactive phenols in red wine.
title_sort Solid cation exchange phase to remove interfering anthocyanins in the analysis of other bioactive phenols in red wine.
author SILVA, L. F. da
author_facet SILVA, L. F. da
GUERRA, C. C.
KLEIN, D.
BERGOLD, A. M.
author_role author
author2 GUERRA, C. C.
KLEIN, D.
BERGOLD, A. M.
author2_role author
author
author
dc.contributor.none.fl_str_mv LETICIA FLORES DA SILVA, CNPUV; CELITO CRIVELLARO GUERRA, CNPUV; Diandra Klein, Faculdade de Farmácia, Universidade de Caxias do Sul; Ana Maria Bergold, Laboratório de Química Farmacêutica, Universidade Federal do Rio Grande do Sul.
dc.contributor.author.fl_str_mv SILVA, L. F. da
GUERRA, C. C.
KLEIN, D.
BERGOLD, A. M.
dc.subject.por.fl_str_mv Sample purification
Bioactive phenols
Diode array detector
Mass spectometry
Red wine
Liquid Chromatography
topic Sample purification
Bioactive phenols
Diode array detector
Mass spectometry
Red wine
Liquid Chromatography
description Bioactive phenols (BPs) are often targets in red wine analysis. However, other compounds interfere in the liquid chromatography methods used for this analysis. Here, purification procedures were tested to eliminate anthocyanin interference during the determination of 19 red-wine BPs. Liquid chromatography, coupled to a diode array detector (HPLC-DAD) and a mass spectrometer (UPLC-MS), was used to compare the direct injection of the samples with solid-phase extractions: reversed-phase (C18) and strong cation-exchange (SCX). The HPLC-DAD method revealed that, out of 13 BPs, only six are selectively analyzed with or without C18 treatment, whereas SCX enabled the detection of all BPs. The recovery with SCX was above 86.6% for eight BPs. Moreover, UPLC-MS demonstrated the potential of SCX sample preparation for the determination of 19 BPs. The developed procedure may be extended to the analysis of other red wine molecules or to other analytical methods where anthocyanins may interfere. Chemical compounds studied in this article trans-Resveratrol (PubChemCID: 445154); trans-?-Viniferin (PubChemCID: 5315233); Quercetin (PubChemCID: 5280343); Myricetin (PubChemCID: 5281672); Kaempferol (PubChemCID: 5280863); trans-Cinnamic acid (PubChemCID: 444539); trans-Piceid (PubChemCID: 5281718); Taxifolin (PubChemCID: 439533) Keywords Bioactive phenols; Red wine; Liquid chromatography; Mass spectrometry; Diode array detector; Sample purification
publishDate 2017
dc.date.none.fl_str_mv 2017-02-07T11:11:11Z
2017-02-07T11:11:11Z
2017-02-07
2017
2019-05-06T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Food Chemistryl, v. 227, p. 158-165, 2017.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1062946
http://dx.doi.org/10.1016/j.foodchem.2017.01.087
identifier_str_mv Food Chemistryl, v. 227, p. 158-165, 2017.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1062946
http://dx.doi.org/10.1016/j.foodchem.2017.01.087
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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