Tailoring swelling of alginate-gelatin hydrogel microspheres by crosslinking with calcium chloride combined with transglutaminase
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/20227 |
Resumo: | lginate-based hydrogels can find uses in a wide range of applications, including in the encapsulation field. This type of hydrogels is usually ionically crosslinked using calcium sources giving rise to products with limited internal crosslinking. In this work, it is hypothesized that the combination of alginate crosslinked by calcium chloride (external crosslinking; ionic mechanism) with gelatin crosslinked by transglutaminase (internal crosslinking; enzymatic induced mechanism) can be used to tailor the swelling behavior of alginate-based hydrogel microspheres. A systematic study was conducted by covering process variables such as gelatin content, TGase concentration, and CaCl2 contact time, added by statistic tools as central composite rotatable design (CCRD), principal component analysis (PCA) and multiobjective optimization, to map their effect on the resulting water content after production (expressed as swelling ratio), and swelling properties at pH 3 and 7. Among the studied variables, particle's swelling was mostly affected by the gelatin content and transglutaminase concentration. |
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7160 |
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Tailoring swelling of alginate-gelatin hydrogel microspheres by crosslinking with calcium chloride combined with transglutaminaseAlginateEnzymatic-induced crosslinkingGelatinHydrogel microspheresIonic crosslinkingSwellinglginate-based hydrogels can find uses in a wide range of applications, including in the encapsulation field. This type of hydrogels is usually ionically crosslinked using calcium sources giving rise to products with limited internal crosslinking. In this work, it is hypothesized that the combination of alginate crosslinked by calcium chloride (external crosslinking; ionic mechanism) with gelatin crosslinked by transglutaminase (internal crosslinking; enzymatic induced mechanism) can be used to tailor the swelling behavior of alginate-based hydrogel microspheres. A systematic study was conducted by covering process variables such as gelatin content, TGase concentration, and CaCl2 contact time, added by statistic tools as central composite rotatable design (CCRD), principal component analysis (PCA) and multiobjective optimization, to map their effect on the resulting water content after production (expressed as swelling ratio), and swelling properties at pH 3 and 7. Among the studied variables, particle's swelling was mostly affected by the gelatin content and transglutaminase concentration.This work was financially supported by Associate Laboratory LSRELCM (UID/EQU/50020/2019) funded by national funds through FCT/ MCTES (PIDDAC), and Foundation for Science and Technology (FCT, Portugal) and by CIMO (UID/AGR/00690/2019) trough FEDER under Program PT2020. The authors are grateful to CAPES, CNPq and Fundação Araucária for the support and also to Ajinomoto Foods Europe S.A.S. (France) for kindly provide the transglutaminase sample used in this work. I.P. Fernandes thanks the national funding by FCT, P.I., through the institutional scientific employment program-contract for her contract.Biblioteca Digital do IPBPilipenko, NadezhdaGonçalves, Odinei HessBona, EvandroFernandes, Isabel P.Pinto, João A.Sorita, Guilherme DallarmiLeimann, Fernanda VitóriaBarreiro, M.F.2018-01-19T10:00:00Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/20227engPilipenko, Nadezhda; Gonçalves, Odinei Hess; Bona, Evandro; Fernandes, Isabel P.; Pinto, João A.; Sorita, Guilherme D.; Leimann, Fernanda Vitória; Barreiro, Maria Filomena (2019). Tailoring swelling of alginate-gelatin hydrogel microspheres by crosslinking with calcium chloride combined with transglutaminase. Carbohydrate Polymers. ISSN 0144-8617. 223, p. 1-90144-861710.1016/j.carbpol.2019.115035info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:46:05Zoai:bibliotecadigital.ipb.pt:10198/20227Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:10:57.867516Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Tailoring swelling of alginate-gelatin hydrogel microspheres by crosslinking with calcium chloride combined with transglutaminase |
title |
Tailoring swelling of alginate-gelatin hydrogel microspheres by crosslinking with calcium chloride combined with transglutaminase |
spellingShingle |
Tailoring swelling of alginate-gelatin hydrogel microspheres by crosslinking with calcium chloride combined with transglutaminase Pilipenko, Nadezhda Alginate Enzymatic-induced crosslinking Gelatin Hydrogel microspheres Ionic crosslinking Swelling |
title_short |
Tailoring swelling of alginate-gelatin hydrogel microspheres by crosslinking with calcium chloride combined with transglutaminase |
title_full |
Tailoring swelling of alginate-gelatin hydrogel microspheres by crosslinking with calcium chloride combined with transglutaminase |
title_fullStr |
Tailoring swelling of alginate-gelatin hydrogel microspheres by crosslinking with calcium chloride combined with transglutaminase |
title_full_unstemmed |
Tailoring swelling of alginate-gelatin hydrogel microspheres by crosslinking with calcium chloride combined with transglutaminase |
title_sort |
Tailoring swelling of alginate-gelatin hydrogel microspheres by crosslinking with calcium chloride combined with transglutaminase |
author |
Pilipenko, Nadezhda |
author_facet |
Pilipenko, Nadezhda Gonçalves, Odinei Hess Bona, Evandro Fernandes, Isabel P. Pinto, João A. Sorita, Guilherme Dallarmi Leimann, Fernanda Vitória Barreiro, M.F. |
author_role |
author |
author2 |
Gonçalves, Odinei Hess Bona, Evandro Fernandes, Isabel P. Pinto, João A. Sorita, Guilherme Dallarmi Leimann, Fernanda Vitória Barreiro, M.F. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Pilipenko, Nadezhda Gonçalves, Odinei Hess Bona, Evandro Fernandes, Isabel P. Pinto, João A. Sorita, Guilherme Dallarmi Leimann, Fernanda Vitória Barreiro, M.F. |
dc.subject.por.fl_str_mv |
Alginate Enzymatic-induced crosslinking Gelatin Hydrogel microspheres Ionic crosslinking Swelling |
topic |
Alginate Enzymatic-induced crosslinking Gelatin Hydrogel microspheres Ionic crosslinking Swelling |
description |
lginate-based hydrogels can find uses in a wide range of applications, including in the encapsulation field. This type of hydrogels is usually ionically crosslinked using calcium sources giving rise to products with limited internal crosslinking. In this work, it is hypothesized that the combination of alginate crosslinked by calcium chloride (external crosslinking; ionic mechanism) with gelatin crosslinked by transglutaminase (internal crosslinking; enzymatic induced mechanism) can be used to tailor the swelling behavior of alginate-based hydrogel microspheres. A systematic study was conducted by covering process variables such as gelatin content, TGase concentration, and CaCl2 contact time, added by statistic tools as central composite rotatable design (CCRD), principal component analysis (PCA) and multiobjective optimization, to map their effect on the resulting water content after production (expressed as swelling ratio), and swelling properties at pH 3 and 7. Among the studied variables, particle's swelling was mostly affected by the gelatin content and transglutaminase concentration. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-19T10:00:00Z 2019 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/20227 |
url |
http://hdl.handle.net/10198/20227 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pilipenko, Nadezhda; Gonçalves, Odinei Hess; Bona, Evandro; Fernandes, Isabel P.; Pinto, João A.; Sorita, Guilherme D.; Leimann, Fernanda Vitória; Barreiro, Maria Filomena (2019). Tailoring swelling of alginate-gelatin hydrogel microspheres by crosslinking with calcium chloride combined with transglutaminase. Carbohydrate Polymers. ISSN 0144-8617. 223, p. 1-9 0144-8617 10.1016/j.carbpol.2019.115035 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799135376583950336 |