Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/148285 |
Resumo: | Funding Information: The National Surveillance of Diarrhea was supported by a Senior Fellowship awarded to Nilsa de Deus by the European Foundations Initiative for African Research into Neglected Tropical Diseases (EFINTD, grant number 98539), the World Health Organization, a Master Fellowship funded by the Italian Agency for Development Cooperation (AICS) project AID10524, Deutsche Forschungsgemeinschaft (DFG, grant number JO369/5–1)—where Adilson Fernando Loforte Bauhofer and Assucênio Chissaque are fellows, GAVI (Global Alliance for Vaccines and Immunization) through Health System Strengthening (HSS), and Fundo Nacional de Investigação (FNI). The protocol was approved by the National Bioethics Committee for Health of Mozambique (IRB00002657, reference number: 348/CNBS/13), and each caregiver gave written informed consent to authorize their child's participation. The authors want to thank the parents or guardians who consented to their children's enrollment in the surveillance program. The authors acknowledge Dr. Octávio Jossai, the National Reference Laboratory of Microbiology team, all the focal points, and the provincial field teams who helped to conduct this study. Funding Information: The National Surveillance of Diarrhea was supported by a Senior Fellowship awarded to Nilsa de Deus by the European Foundations Initiative for African Research into Neglected Tropical Diseases (EFINTD, grant number 98539), the World Health Organization, a Master Fellowship funded by the Italian Agency for Development Cooperation (AICS) project AID10524, Deutsche Forschungsgemeinschaft (DFG, grant number JO369/5–1)—where Adilson Fernando Loforte Bauhofer and Assucênio Chissaque are fellows, GAVI (Global Alliance for Vaccines and Immunization) through Health System Strengthening (HSS), and Fundo Nacional de Investigação (FNI). Publisher Copyright: © 2022 |
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Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrheaA cross-sectional study in four provinces of Mozambique: Diarrheagenic Escherichia coli in MozambiqueAntibiotics resistanceChildrenDiarrheagenic E. coli pathotypesEscherichia coliMozambiqueR MedicineQR MicrobiologyRM Therapeutics. PharmacologyRS Pharmacy and materia medicaMicrobiology (medical)Infectious DiseasesSDG 3 - Good Health and Well-beingFunding Information: The National Surveillance of Diarrhea was supported by a Senior Fellowship awarded to Nilsa de Deus by the European Foundations Initiative for African Research into Neglected Tropical Diseases (EFINTD, grant number 98539), the World Health Organization, a Master Fellowship funded by the Italian Agency for Development Cooperation (AICS) project AID10524, Deutsche Forschungsgemeinschaft (DFG, grant number JO369/5–1)—where Adilson Fernando Loforte Bauhofer and Assucênio Chissaque are fellows, GAVI (Global Alliance for Vaccines and Immunization) through Health System Strengthening (HSS), and Fundo Nacional de Investigação (FNI). The protocol was approved by the National Bioethics Committee for Health of Mozambique (IRB00002657, reference number: 348/CNBS/13), and each caregiver gave written informed consent to authorize their child's participation. The authors want to thank the parents or guardians who consented to their children's enrollment in the surveillance program. The authors acknowledge Dr. Octávio Jossai, the National Reference Laboratory of Microbiology team, all the focal points, and the provincial field teams who helped to conduct this study. Funding Information: The National Surveillance of Diarrhea was supported by a Senior Fellowship awarded to Nilsa de Deus by the European Foundations Initiative for African Research into Neglected Tropical Diseases (EFINTD, grant number 98539), the World Health Organization, a Master Fellowship funded by the Italian Agency for Development Cooperation (AICS) project AID10524, Deutsche Forschungsgemeinschaft (DFG, grant number JO369/5–1)—where Adilson Fernando Loforte Bauhofer and Assucênio Chissaque are fellows, GAVI (Global Alliance for Vaccines and Immunization) through Health System Strengthening (HSS), and Fundo Nacional de Investigação (FNI). Publisher Copyright: © 2022Objectives: Analyze the frequency of diarrheagenic Escherichia coli (DEC) pathotypes and their antimicrobial resistance profiles among children aged <15 years with diarrhea in four Mozambican provinces. Methods: A cross-sectional hospital-based surveillance program of diarrhea was implemented in Maputo, Sofala, Zambézia, and Nampula. A single stool sample was collected from each child from May 2014 to May 2017. Culture methods and biochemical characterization were performed to detect E. coli strains. DEC pathotypes were determined by conventional polymerase chain reaction targeting specific virulence genes. Antimicrobial susceptibility was assessed by the Kirby–Bauer method. Results: From 723 specimens analyzed by culture, 262 were positive for E. coli. A total of 208 samples were tested by polymerase chain reaction for DEC identification, of which 101 (48.6%) were positive for a DEC pathotype. The predominant pathotypes were enteroaggregative (66.3%, 67/101), enteropathogenic (15.8%, 16/101), enterotoxigenic (13.9%, 14/101), and enteroinvasive E. coli (4.0%, 4/101). No Shiga toxin–producing E. coli was identified. Regardless of the province, the most frequent pathotype was enteroaggregative E. coli. Isolated DEC presented high frequency of resistance to ampicillin (97.8%), tetracycline (68.3%), chloramphenicol (28.4%), nalidixic acid (19.5%), and gentamicin (14.4%). Conclusion: Children with diarrhea in Mozambique had DEC and higher resistance to ampicillin and tetracycline.Instituto de Higiene e Medicina Tropical (IHMT)RUNManhique-Coutinho, LenaChiani, PaolaMichelacci, ValeriaTaviani, ElisaBauhofer, Adilson Fernando LoforteChissaque, AssucênioCossa-Moiane, IdaléciaSambo, JúliaChilaúle, JorféliaGuimarães, Esperança LourençoSalência, JuditeCassocera, MartaBero, Diocreciano MatiasLanga, José Paulode Deus, Nilsa2023-01-27T22:19:38Z2022-082022-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5application/pdfhttp://hdl.handle.net/10362/148285eng1201-9712PURE: 50808284https://doi.org/10.1016/j.ijid.2022.04.054info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:29:41Zoai:run.unl.pt:10362/148285Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:53:18.611512Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea A cross-sectional study in four provinces of Mozambique: Diarrheagenic Escherichia coli in Mozambique |
title |
Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea |
spellingShingle |
Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea Manhique-Coutinho, Lena Antibiotics resistance Children Diarrheagenic E. coli pathotypes Escherichia coli Mozambique R Medicine QR Microbiology RM Therapeutics. Pharmacology RS Pharmacy and materia medica Microbiology (medical) Infectious Diseases SDG 3 - Good Health and Well-being |
title_short |
Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea |
title_full |
Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea |
title_fullStr |
Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea |
title_full_unstemmed |
Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea |
title_sort |
Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea |
author |
Manhique-Coutinho, Lena |
author_facet |
Manhique-Coutinho, Lena Chiani, Paola Michelacci, Valeria Taviani, Elisa Bauhofer, Adilson Fernando Loforte Chissaque, Assucênio Cossa-Moiane, Idalécia Sambo, Júlia Chilaúle, Jorfélia Guimarães, Esperança Lourenço Salência, Judite Cassocera, Marta Bero, Diocreciano Matias Langa, José Paulo de Deus, Nilsa |
author_role |
author |
author2 |
Chiani, Paola Michelacci, Valeria Taviani, Elisa Bauhofer, Adilson Fernando Loforte Chissaque, Assucênio Cossa-Moiane, Idalécia Sambo, Júlia Chilaúle, Jorfélia Guimarães, Esperança Lourenço Salência, Judite Cassocera, Marta Bero, Diocreciano Matias Langa, José Paulo de Deus, Nilsa |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Instituto de Higiene e Medicina Tropical (IHMT) RUN |
dc.contributor.author.fl_str_mv |
Manhique-Coutinho, Lena Chiani, Paola Michelacci, Valeria Taviani, Elisa Bauhofer, Adilson Fernando Loforte Chissaque, Assucênio Cossa-Moiane, Idalécia Sambo, Júlia Chilaúle, Jorfélia Guimarães, Esperança Lourenço Salência, Judite Cassocera, Marta Bero, Diocreciano Matias Langa, José Paulo de Deus, Nilsa |
dc.subject.por.fl_str_mv |
Antibiotics resistance Children Diarrheagenic E. coli pathotypes Escherichia coli Mozambique R Medicine QR Microbiology RM Therapeutics. Pharmacology RS Pharmacy and materia medica Microbiology (medical) Infectious Diseases SDG 3 - Good Health and Well-being |
topic |
Antibiotics resistance Children Diarrheagenic E. coli pathotypes Escherichia coli Mozambique R Medicine QR Microbiology RM Therapeutics. Pharmacology RS Pharmacy and materia medica Microbiology (medical) Infectious Diseases SDG 3 - Good Health and Well-being |
description |
Funding Information: The National Surveillance of Diarrhea was supported by a Senior Fellowship awarded to Nilsa de Deus by the European Foundations Initiative for African Research into Neglected Tropical Diseases (EFINTD, grant number 98539), the World Health Organization, a Master Fellowship funded by the Italian Agency for Development Cooperation (AICS) project AID10524, Deutsche Forschungsgemeinschaft (DFG, grant number JO369/5–1)—where Adilson Fernando Loforte Bauhofer and Assucênio Chissaque are fellows, GAVI (Global Alliance for Vaccines and Immunization) through Health System Strengthening (HSS), and Fundo Nacional de Investigação (FNI). The protocol was approved by the National Bioethics Committee for Health of Mozambique (IRB00002657, reference number: 348/CNBS/13), and each caregiver gave written informed consent to authorize their child's participation. The authors want to thank the parents or guardians who consented to their children's enrollment in the surveillance program. The authors acknowledge Dr. Octávio Jossai, the National Reference Laboratory of Microbiology team, all the focal points, and the provincial field teams who helped to conduct this study. Funding Information: The National Surveillance of Diarrhea was supported by a Senior Fellowship awarded to Nilsa de Deus by the European Foundations Initiative for African Research into Neglected Tropical Diseases (EFINTD, grant number 98539), the World Health Organization, a Master Fellowship funded by the Italian Agency for Development Cooperation (AICS) project AID10524, Deutsche Forschungsgemeinschaft (DFG, grant number JO369/5–1)—where Adilson Fernando Loforte Bauhofer and Assucênio Chissaque are fellows, GAVI (Global Alliance for Vaccines and Immunization) through Health System Strengthening (HSS), and Fundo Nacional de Investigação (FNI). Publisher Copyright: © 2022 |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08 2022-08-01T00:00:00Z 2023-01-27T22:19:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/148285 |
url |
http://hdl.handle.net/10362/148285 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1201-9712 PURE: 50808284 https://doi.org/10.1016/j.ijid.2022.04.054 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
5 application/pdf |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799138123831050240 |