Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea

Detalhes bibliográficos
Autor(a) principal: Manhique-Coutinho, Lena
Data de Publicação: 2022
Outros Autores: Chiani, Paola, Michelacci, Valeria, Taviani, Elisa, Bauhofer, Adilson Fernando Loforte, Chissaque, Assucênio, Cossa-Moiane, Idalécia, Sambo, Júlia, Chilaúle, Jorfélia, Guimarães, Esperança Lourenço, Salência, Judite, Cassocera, Marta, Bero, Diocreciano Matias, Langa, José Paulo, de Deus, Nilsa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/148285
Resumo: Funding Information: The National Surveillance of Diarrhea was supported by a Senior Fellowship awarded to Nilsa de Deus by the European Foundations Initiative for African Research into Neglected Tropical Diseases (EFINTD, grant number 98539), the World Health Organization, a Master Fellowship funded by the Italian Agency for Development Cooperation (AICS) project AID10524, Deutsche Forschungsgemeinschaft (DFG, grant number JO369/5–1)—where Adilson Fernando Loforte Bauhofer and Assucênio Chissaque are fellows, GAVI (Global Alliance for Vaccines and Immunization) through Health System Strengthening (HSS), and Fundo Nacional de Investigação (FNI). The protocol was approved by the National Bioethics Committee for Health of Mozambique (IRB00002657, reference number: 348/CNBS/13), and each caregiver gave written informed consent to authorize their child's participation. The authors want to thank the parents or guardians who consented to their children's enrollment in the surveillance program. The authors acknowledge Dr. Octávio Jossai, the National Reference Laboratory of Microbiology team, all the focal points, and the provincial field teams who helped to conduct this study. Funding Information: The National Surveillance of Diarrhea was supported by a Senior Fellowship awarded to Nilsa de Deus by the European Foundations Initiative for African Research into Neglected Tropical Diseases (EFINTD, grant number 98539), the World Health Organization, a Master Fellowship funded by the Italian Agency for Development Cooperation (AICS) project AID10524, Deutsche Forschungsgemeinschaft (DFG, grant number JO369/5–1)—where Adilson Fernando Loforte Bauhofer and Assucênio Chissaque are fellows, GAVI (Global Alliance for Vaccines and Immunization) through Health System Strengthening (HSS), and Fundo Nacional de Investigação (FNI). Publisher Copyright: © 2022
id RCAP_382d81b41a6de4c1640715717e4a9bdc
oai_identifier_str oai:run.unl.pt:10362/148285
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrheaA cross-sectional study in four provinces of Mozambique: Diarrheagenic Escherichia coli in MozambiqueAntibiotics resistanceChildrenDiarrheagenic E. coli pathotypesEscherichia coliMozambiqueR MedicineQR MicrobiologyRM Therapeutics. PharmacologyRS Pharmacy and materia medicaMicrobiology (medical)Infectious DiseasesSDG 3 - Good Health and Well-beingFunding Information: The National Surveillance of Diarrhea was supported by a Senior Fellowship awarded to Nilsa de Deus by the European Foundations Initiative for African Research into Neglected Tropical Diseases (EFINTD, grant number 98539), the World Health Organization, a Master Fellowship funded by the Italian Agency for Development Cooperation (AICS) project AID10524, Deutsche Forschungsgemeinschaft (DFG, grant number JO369/5–1)—where Adilson Fernando Loforte Bauhofer and Assucênio Chissaque are fellows, GAVI (Global Alliance for Vaccines and Immunization) through Health System Strengthening (HSS), and Fundo Nacional de Investigação (FNI). The protocol was approved by the National Bioethics Committee for Health of Mozambique (IRB00002657, reference number: 348/CNBS/13), and each caregiver gave written informed consent to authorize their child's participation. The authors want to thank the parents or guardians who consented to their children's enrollment in the surveillance program. The authors acknowledge Dr. Octávio Jossai, the National Reference Laboratory of Microbiology team, all the focal points, and the provincial field teams who helped to conduct this study. Funding Information: The National Surveillance of Diarrhea was supported by a Senior Fellowship awarded to Nilsa de Deus by the European Foundations Initiative for African Research into Neglected Tropical Diseases (EFINTD, grant number 98539), the World Health Organization, a Master Fellowship funded by the Italian Agency for Development Cooperation (AICS) project AID10524, Deutsche Forschungsgemeinschaft (DFG, grant number JO369/5–1)—where Adilson Fernando Loforte Bauhofer and Assucênio Chissaque are fellows, GAVI (Global Alliance for Vaccines and Immunization) through Health System Strengthening (HSS), and Fundo Nacional de Investigação (FNI). Publisher Copyright: © 2022Objectives: Analyze the frequency of diarrheagenic Escherichia coli (DEC) pathotypes and their antimicrobial resistance profiles among children aged <15 years with diarrhea in four Mozambican provinces. Methods: A cross-sectional hospital-based surveillance program of diarrhea was implemented in Maputo, Sofala, Zambézia, and Nampula. A single stool sample was collected from each child from May 2014 to May 2017. Culture methods and biochemical characterization were performed to detect E. coli strains. DEC pathotypes were determined by conventional polymerase chain reaction targeting specific virulence genes. Antimicrobial susceptibility was assessed by the Kirby–Bauer method. Results: From 723 specimens analyzed by culture, 262 were positive for E. coli. A total of 208 samples were tested by polymerase chain reaction for DEC identification, of which 101 (48.6%) were positive for a DEC pathotype. The predominant pathotypes were enteroaggregative (66.3%, 67/101), enteropathogenic (15.8%, 16/101), enterotoxigenic (13.9%, 14/101), and enteroinvasive E. coli (4.0%, 4/101). No Shiga toxin–producing E. coli was identified. Regardless of the province, the most frequent pathotype was enteroaggregative E. coli. Isolated DEC presented high frequency of resistance to ampicillin (97.8%), tetracycline (68.3%), chloramphenicol (28.4%), nalidixic acid (19.5%), and gentamicin (14.4%). Conclusion: Children with diarrhea in Mozambique had DEC and higher resistance to ampicillin and tetracycline.Instituto de Higiene e Medicina Tropical (IHMT)RUNManhique-Coutinho, LenaChiani, PaolaMichelacci, ValeriaTaviani, ElisaBauhofer, Adilson Fernando LoforteChissaque, AssucênioCossa-Moiane, IdaléciaSambo, JúliaChilaúle, JorféliaGuimarães, Esperança LourençoSalência, JuditeCassocera, MartaBero, Diocreciano MatiasLanga, José Paulode Deus, Nilsa2023-01-27T22:19:38Z2022-082022-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5application/pdfhttp://hdl.handle.net/10362/148285eng1201-9712PURE: 50808284https://doi.org/10.1016/j.ijid.2022.04.054info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:29:41Zoai:run.unl.pt:10362/148285Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:53:18.611512Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea
A cross-sectional study in four provinces of Mozambique: Diarrheagenic Escherichia coli in Mozambique
title Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea
spellingShingle Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea
Manhique-Coutinho, Lena
Antibiotics resistance
Children
Diarrheagenic E. coli pathotypes
Escherichia coli
Mozambique
R Medicine
QR Microbiology
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
title_short Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea
title_full Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea
title_fullStr Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea
title_full_unstemmed Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea
title_sort Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea
author Manhique-Coutinho, Lena
author_facet Manhique-Coutinho, Lena
Chiani, Paola
Michelacci, Valeria
Taviani, Elisa
Bauhofer, Adilson Fernando Loforte
Chissaque, Assucênio
Cossa-Moiane, Idalécia
Sambo, Júlia
Chilaúle, Jorfélia
Guimarães, Esperança Lourenço
Salência, Judite
Cassocera, Marta
Bero, Diocreciano Matias
Langa, José Paulo
de Deus, Nilsa
author_role author
author2 Chiani, Paola
Michelacci, Valeria
Taviani, Elisa
Bauhofer, Adilson Fernando Loforte
Chissaque, Assucênio
Cossa-Moiane, Idalécia
Sambo, Júlia
Chilaúle, Jorfélia
Guimarães, Esperança Lourenço
Salência, Judite
Cassocera, Marta
Bero, Diocreciano Matias
Langa, José Paulo
de Deus, Nilsa
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Higiene e Medicina Tropical (IHMT)
RUN
dc.contributor.author.fl_str_mv Manhique-Coutinho, Lena
Chiani, Paola
Michelacci, Valeria
Taviani, Elisa
Bauhofer, Adilson Fernando Loforte
Chissaque, Assucênio
Cossa-Moiane, Idalécia
Sambo, Júlia
Chilaúle, Jorfélia
Guimarães, Esperança Lourenço
Salência, Judite
Cassocera, Marta
Bero, Diocreciano Matias
Langa, José Paulo
de Deus, Nilsa
dc.subject.por.fl_str_mv Antibiotics resistance
Children
Diarrheagenic E. coli pathotypes
Escherichia coli
Mozambique
R Medicine
QR Microbiology
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
topic Antibiotics resistance
Children
Diarrheagenic E. coli pathotypes
Escherichia coli
Mozambique
R Medicine
QR Microbiology
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
description Funding Information: The National Surveillance of Diarrhea was supported by a Senior Fellowship awarded to Nilsa de Deus by the European Foundations Initiative for African Research into Neglected Tropical Diseases (EFINTD, grant number 98539), the World Health Organization, a Master Fellowship funded by the Italian Agency for Development Cooperation (AICS) project AID10524, Deutsche Forschungsgemeinschaft (DFG, grant number JO369/5–1)—where Adilson Fernando Loforte Bauhofer and Assucênio Chissaque are fellows, GAVI (Global Alliance for Vaccines and Immunization) through Health System Strengthening (HSS), and Fundo Nacional de Investigação (FNI). The protocol was approved by the National Bioethics Committee for Health of Mozambique (IRB00002657, reference number: 348/CNBS/13), and each caregiver gave written informed consent to authorize their child's participation. The authors want to thank the parents or guardians who consented to their children's enrollment in the surveillance program. The authors acknowledge Dr. Octávio Jossai, the National Reference Laboratory of Microbiology team, all the focal points, and the provincial field teams who helped to conduct this study. Funding Information: The National Surveillance of Diarrhea was supported by a Senior Fellowship awarded to Nilsa de Deus by the European Foundations Initiative for African Research into Neglected Tropical Diseases (EFINTD, grant number 98539), the World Health Organization, a Master Fellowship funded by the Italian Agency for Development Cooperation (AICS) project AID10524, Deutsche Forschungsgemeinschaft (DFG, grant number JO369/5–1)—where Adilson Fernando Loforte Bauhofer and Assucênio Chissaque are fellows, GAVI (Global Alliance for Vaccines and Immunization) through Health System Strengthening (HSS), and Fundo Nacional de Investigação (FNI). Publisher Copyright: © 2022
publishDate 2022
dc.date.none.fl_str_mv 2022-08
2022-08-01T00:00:00Z
2023-01-27T22:19:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/148285
url http://hdl.handle.net/10362/148285
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1201-9712
PURE: 50808284
https://doi.org/10.1016/j.ijid.2022.04.054
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 5
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138123831050240