Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology

Detalhes bibliográficos
Autor(a) principal: Taofiq, Oludemi
Data de Publicação: 2018
Outros Autores: Silva, Ana Rita, Costa, Cristina, Ferreira, Inês, Nunes, João, Prieto Lage, Miguel A., Simal-Gandara, Jesus, Barros, Lillian, Ferreira, Isabel C.F.R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/23407
Resumo: In this study, heat-assisted extraction (HAE) was used to maximize ergosterol extraction from Pleurotus ostreatus (PO) and Pleurotus eryngii (PE) using response surface methodology (RSM). Different temperature (T) and time (t) conditions were applied to understand their influence on the extraction yield (Y1), ergosterol purity in the extracted material (mg g-1 R, Y2) and ergosterol content in the two Pleurotus species (mg per 100 g dw, Y3). A circumscribed central composite design was used to evaluate the interactive effects of extraction variables and the optimal conditions were determined using second-order polynomial mathematical models to describe the responses obtained. In all cases, the predicted responses showed satisfactory fitting between the predicted and experimental values (R2 values >0.8). The global optimum conditions predicted by the models were for PO at T = 54.3 °C and t = 150 min (yielded 7.25%, 33.32 mg E per g R and 244.25 mg E per 100 g dw), while for PE at T = 61.8 °C and t = 150 min (yielded 8.02%, 43.63 mg E per g R and 360.58 mg E per 100 g dw). The obtained results from the two Pleurotus mushroom species using HAE show the possibilities of using them as a production source of enriched extracts in ergosterol.
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spelling Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodologyIn this study, heat-assisted extraction (HAE) was used to maximize ergosterol extraction from Pleurotus ostreatus (PO) and Pleurotus eryngii (PE) using response surface methodology (RSM). Different temperature (T) and time (t) conditions were applied to understand their influence on the extraction yield (Y1), ergosterol purity in the extracted material (mg g-1 R, Y2) and ergosterol content in the two Pleurotus species (mg per 100 g dw, Y3). A circumscribed central composite design was used to evaluate the interactive effects of extraction variables and the optimal conditions were determined using second-order polynomial mathematical models to describe the responses obtained. In all cases, the predicted responses showed satisfactory fitting between the predicted and experimental values (R2 values >0.8). The global optimum conditions predicted by the models were for PO at T = 54.3 °C and t = 150 min (yielded 7.25%, 33.32 mg E per g R and 244.25 mg E per 100 g dw), while for PE at T = 61.8 °C and t = 150 min (yielded 8.02%, 43.63 mg E per g R and 360.58 mg E per 100 g dw). The obtained results from the two Pleurotus mushroom species using HAE show the possibilities of using them as a production source of enriched extracts in ergosterol.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020); this work was also funded by the European Agricultural Fund for Rural Development (EAFRD), through the Rural Development Program (PDR2020), within the scope of the project MicoCoating (PDR2020-101-03147). L. Barros also acknowledges the national funding by FCT, P.I., through the institutional scientific employment program-contract for their contracts. MICINN is acknowledged for the financial support for the Ramón&Cajal researcher, M.A. Prieto.Biblioteca Digital do IPBTaofiq, OludemiSilva, Ana RitaCosta, CristinaFerreira, InêsNunes, JoãoPrieto Lage, Miguel A.Simal-Gandara, JesusBarros, LillianFerreira, Isabel C.F.R.2018-01-19T10:00:00Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/23407engTaofiq, Oludemi; Silva, Ana Rita; Costa, Cristina; Ferreira, Inês; Nunes, João; Prieto, Miguel A.; Simal-Gandara, Jesús; Barros, Lillian; Ferreira, Isabel C.F.R. (2020). Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology. Food and Function. ISSN 2042-6496. 11:7, p. 5887-58972042-649610.1039/d0fo00301hinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:52:20Zoai:bibliotecadigital.ipb.pt:10198/23407Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:14:24.497847Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology
title Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology
spellingShingle Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology
Taofiq, Oludemi
title_short Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology
title_full Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology
title_fullStr Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology
title_full_unstemmed Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology
title_sort Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology
author Taofiq, Oludemi
author_facet Taofiq, Oludemi
Silva, Ana Rita
Costa, Cristina
Ferreira, Inês
Nunes, João
Prieto Lage, Miguel A.
Simal-Gandara, Jesus
Barros, Lillian
Ferreira, Isabel C.F.R.
author_role author
author2 Silva, Ana Rita
Costa, Cristina
Ferreira, Inês
Nunes, João
Prieto Lage, Miguel A.
Simal-Gandara, Jesus
Barros, Lillian
Ferreira, Isabel C.F.R.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Taofiq, Oludemi
Silva, Ana Rita
Costa, Cristina
Ferreira, Inês
Nunes, João
Prieto Lage, Miguel A.
Simal-Gandara, Jesus
Barros, Lillian
Ferreira, Isabel C.F.R.
description In this study, heat-assisted extraction (HAE) was used to maximize ergosterol extraction from Pleurotus ostreatus (PO) and Pleurotus eryngii (PE) using response surface methodology (RSM). Different temperature (T) and time (t) conditions were applied to understand their influence on the extraction yield (Y1), ergosterol purity in the extracted material (mg g-1 R, Y2) and ergosterol content in the two Pleurotus species (mg per 100 g dw, Y3). A circumscribed central composite design was used to evaluate the interactive effects of extraction variables and the optimal conditions were determined using second-order polynomial mathematical models to describe the responses obtained. In all cases, the predicted responses showed satisfactory fitting between the predicted and experimental values (R2 values >0.8). The global optimum conditions predicted by the models were for PO at T = 54.3 °C and t = 150 min (yielded 7.25%, 33.32 mg E per g R and 244.25 mg E per 100 g dw), while for PE at T = 61.8 °C and t = 150 min (yielded 8.02%, 43.63 mg E per g R and 360.58 mg E per 100 g dw). The obtained results from the two Pleurotus mushroom species using HAE show the possibilities of using them as a production source of enriched extracts in ergosterol.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-19T10:00:00Z
2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/23407
url http://hdl.handle.net/10198/23407
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Taofiq, Oludemi; Silva, Ana Rita; Costa, Cristina; Ferreira, Inês; Nunes, João; Prieto, Miguel A.; Simal-Gandara, Jesús; Barros, Lillian; Ferreira, Isabel C.F.R. (2020). Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology. Food and Function. ISSN 2042-6496. 11:7, p. 5887-5897
2042-6496
10.1039/d0fo00301h
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