Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/23407 |
Resumo: | In this study, heat-assisted extraction (HAE) was used to maximize ergosterol extraction from Pleurotus ostreatus (PO) and Pleurotus eryngii (PE) using response surface methodology (RSM). Different temperature (T) and time (t) conditions were applied to understand their influence on the extraction yield (Y1), ergosterol purity in the extracted material (mg g-1 R, Y2) and ergosterol content in the two Pleurotus species (mg per 100 g dw, Y3). A circumscribed central composite design was used to evaluate the interactive effects of extraction variables and the optimal conditions were determined using second-order polynomial mathematical models to describe the responses obtained. In all cases, the predicted responses showed satisfactory fitting between the predicted and experimental values (R2 values >0.8). The global optimum conditions predicted by the models were for PO at T = 54.3 °C and t = 150 min (yielded 7.25%, 33.32 mg E per g R and 244.25 mg E per 100 g dw), while for PE at T = 61.8 °C and t = 150 min (yielded 8.02%, 43.63 mg E per g R and 360.58 mg E per 100 g dw). The obtained results from the two Pleurotus mushroom species using HAE show the possibilities of using them as a production source of enriched extracts in ergosterol. |
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Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodologyIn this study, heat-assisted extraction (HAE) was used to maximize ergosterol extraction from Pleurotus ostreatus (PO) and Pleurotus eryngii (PE) using response surface methodology (RSM). Different temperature (T) and time (t) conditions were applied to understand their influence on the extraction yield (Y1), ergosterol purity in the extracted material (mg g-1 R, Y2) and ergosterol content in the two Pleurotus species (mg per 100 g dw, Y3). A circumscribed central composite design was used to evaluate the interactive effects of extraction variables and the optimal conditions were determined using second-order polynomial mathematical models to describe the responses obtained. In all cases, the predicted responses showed satisfactory fitting between the predicted and experimental values (R2 values >0.8). The global optimum conditions predicted by the models were for PO at T = 54.3 °C and t = 150 min (yielded 7.25%, 33.32 mg E per g R and 244.25 mg E per 100 g dw), while for PE at T = 61.8 °C and t = 150 min (yielded 8.02%, 43.63 mg E per g R and 360.58 mg E per 100 g dw). The obtained results from the two Pleurotus mushroom species using HAE show the possibilities of using them as a production source of enriched extracts in ergosterol.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020); this work was also funded by the European Agricultural Fund for Rural Development (EAFRD), through the Rural Development Program (PDR2020), within the scope of the project MicoCoating (PDR2020-101-03147). L. Barros also acknowledges the national funding by FCT, P.I., through the institutional scientific employment program-contract for their contracts. MICINN is acknowledged for the financial support for the Ramón&Cajal researcher, M.A. Prieto.Biblioteca Digital do IPBTaofiq, OludemiSilva, Ana RitaCosta, CristinaFerreira, InêsNunes, JoãoPrieto Lage, Miguel A.Simal-Gandara, JesusBarros, LillianFerreira, Isabel C.F.R.2018-01-19T10:00:00Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/23407engTaofiq, Oludemi; Silva, Ana Rita; Costa, Cristina; Ferreira, Inês; Nunes, João; Prieto, Miguel A.; Simal-Gandara, Jesús; Barros, Lillian; Ferreira, Isabel C.F.R. (2020). Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology. Food and Function. ISSN 2042-6496. 11:7, p. 5887-58972042-649610.1039/d0fo00301hinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:52:20Zoai:bibliotecadigital.ipb.pt:10198/23407Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:14:24.497847Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology |
title |
Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology |
spellingShingle |
Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology Taofiq, Oludemi |
title_short |
Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology |
title_full |
Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology |
title_fullStr |
Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology |
title_full_unstemmed |
Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology |
title_sort |
Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology |
author |
Taofiq, Oludemi |
author_facet |
Taofiq, Oludemi Silva, Ana Rita Costa, Cristina Ferreira, Inês Nunes, João Prieto Lage, Miguel A. Simal-Gandara, Jesus Barros, Lillian Ferreira, Isabel C.F.R. |
author_role |
author |
author2 |
Silva, Ana Rita Costa, Cristina Ferreira, Inês Nunes, João Prieto Lage, Miguel A. Simal-Gandara, Jesus Barros, Lillian Ferreira, Isabel C.F.R. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Taofiq, Oludemi Silva, Ana Rita Costa, Cristina Ferreira, Inês Nunes, João Prieto Lage, Miguel A. Simal-Gandara, Jesus Barros, Lillian Ferreira, Isabel C.F.R. |
description |
In this study, heat-assisted extraction (HAE) was used to maximize ergosterol extraction from Pleurotus ostreatus (PO) and Pleurotus eryngii (PE) using response surface methodology (RSM). Different temperature (T) and time (t) conditions were applied to understand their influence on the extraction yield (Y1), ergosterol purity in the extracted material (mg g-1 R, Y2) and ergosterol content in the two Pleurotus species (mg per 100 g dw, Y3). A circumscribed central composite design was used to evaluate the interactive effects of extraction variables and the optimal conditions were determined using second-order polynomial mathematical models to describe the responses obtained. In all cases, the predicted responses showed satisfactory fitting between the predicted and experimental values (R2 values >0.8). The global optimum conditions predicted by the models were for PO at T = 54.3 °C and t = 150 min (yielded 7.25%, 33.32 mg E per g R and 244.25 mg E per 100 g dw), while for PE at T = 61.8 °C and t = 150 min (yielded 8.02%, 43.63 mg E per g R and 360.58 mg E per 100 g dw). The obtained results from the two Pleurotus mushroom species using HAE show the possibilities of using them as a production source of enriched extracts in ergosterol. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-19T10:00:00Z 2020 2020-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/23407 |
url |
http://hdl.handle.net/10198/23407 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Taofiq, Oludemi; Silva, Ana Rita; Costa, Cristina; Ferreira, Inês; Nunes, João; Prieto, Miguel A.; Simal-Gandara, Jesús; Barros, Lillian; Ferreira, Isabel C.F.R. (2020). Optimization of ergosterol extraction from: Pleurotus mushrooms using response surface methodology. Food and Function. ISSN 2042-6496. 11:7, p. 5887-5897 2042-6496 10.1039/d0fo00301h |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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