3D cytocompatible composites of PCL/Magnetite
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/64609 |
Resumo: | A study of Magnetite (Fe3O4) as a suitable matrix for the improved adhesion and proliferation of MC3T3-E1 pre-osteoblast cells in bone regeneration is presented. Biodegradable and magnetic polycaprolactone (PCL)/magnetite (Fe3O4) scaffolds, which were fabricated by Thermally Induced Phase Separation, are likewise analyzed. Various techniques are used to investigate in vitro degradation at 37 °C, over 104 weeks, in a phosphate buffered saline (PBS) solution. Magnetic measurements that were performed at physiological temperature (310 K) indicated that degradation neither modified the nature nor the distribution of the magnetite nanoparticles. The coercive field strength of the porous matrices demonstrated ferromagnetic behavior and the probable presence of particle interactions. The added nanoparticles facilitated the absorption of PBS, with no considerable increase in matrix degradation rates, as shown by the Gel Permeation Chromatography (GPC) results for Mw, Mn, and I. There was no collapse of the scaffold structures that maintained their structural integrity. Their suitability for bone regeneration was also supported by the absence of matrix cytotoxicity in assays, even after additions of up to 20% magnetite. |
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3D cytocompatible composites of PCL/MagnetitePCLmagnetitescaffoldsmagnetismcytotoxicityin vitro degradationCiências Naturais::Ciências FísicasScience & TechnologyA study of Magnetite (Fe3O4) as a suitable matrix for the improved adhesion and proliferation of MC3T3-E1 pre-osteoblast cells in bone regeneration is presented. Biodegradable and magnetic polycaprolactone (PCL)/magnetite (Fe3O4) scaffolds, which were fabricated by Thermally Induced Phase Separation, are likewise analyzed. Various techniques are used to investigate in vitro degradation at 37 °C, over 104 weeks, in a phosphate buffered saline (PBS) solution. Magnetic measurements that were performed at physiological temperature (310 K) indicated that degradation neither modified the nature nor the distribution of the magnetite nanoparticles. The coercive field strength of the porous matrices demonstrated ferromagnetic behavior and the probable presence of particle interactions. The added nanoparticles facilitated the absorption of PBS, with no considerable increase in matrix degradation rates, as shown by the Gel Permeation Chromatography (GPC) results for Mw, Mn, and I. There was no collapse of the scaffold structures that maintained their structural integrity. Their suitability for bone regeneration was also supported by the absence of matrix cytotoxicity in assays, even after additions of up to 20% magnetite.This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO) through the project MAT2016-76039-C4-3-R (AEI/FEDER, UE) and from the Basque Government Industry Department under the ELKARTEK, HAZITEK and PIBA programs. Supported by the Portuguese Foundation for Science and Technology (FCT) in the framework of the Strategic Funding UID/FIS/04650/2013, project POCI-01-0145-FEDER-028237 and grant SFRH/BD/111478/2015 (S.R.) is acknowledged.Technical and human support provided by SGIker (UPV/EHU, MICINN, GV/EJ, ERDF and ESF) is gratefully appreciated. The authors acknowledge funding by the Spanish Ministry of Economy and Competitiveness (MINECO) through the project MAT2016-76039-C4-3-R (AEI/FEDER, UE) and from the Basque Government Industry and Education Department under the ELKARTEK and HAZITEK and PIBA (PIBA-2018-06) programs. Supported by the Portuguese Foundation for Science and Technology (FCT) in the framework of the Strategic Funding UID/FIS/04650/2013, project POCI-01-0145-FEDER-028237 and grant SFRH/BD/111478/2015 (S.R.) is acknowledged.Multidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoDíaz, E.Valle, M. B.Ribeiro, Sylvie OliveiraLanceros-Méndez, S.Barandiarán, J. M20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/64609engDíaz, E.; Valle, M.B.; Ribeiro, S.; Lanceros-Mendez, S.; Barandiarán, J.M. 3D Cytocompatible Composites of PCL/Magnetite. Materials 2019, 12, 3843.1996-194410.3390/ma12233843https://www.mdpi.com/1996-1944/12/23/3843info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:01:17Zoai:repositorium.sdum.uminho.pt:1822/64609Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:51:12.395712Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
3D cytocompatible composites of PCL/Magnetite |
title |
3D cytocompatible composites of PCL/Magnetite |
spellingShingle |
3D cytocompatible composites of PCL/Magnetite Díaz, E. PCL magnetite scaffolds magnetism cytotoxicity in vitro degradation Ciências Naturais::Ciências Físicas Science & Technology |
title_short |
3D cytocompatible composites of PCL/Magnetite |
title_full |
3D cytocompatible composites of PCL/Magnetite |
title_fullStr |
3D cytocompatible composites of PCL/Magnetite |
title_full_unstemmed |
3D cytocompatible composites of PCL/Magnetite |
title_sort |
3D cytocompatible composites of PCL/Magnetite |
author |
Díaz, E. |
author_facet |
Díaz, E. Valle, M. B. Ribeiro, Sylvie Oliveira Lanceros-Méndez, S. Barandiarán, J. M |
author_role |
author |
author2 |
Valle, M. B. Ribeiro, Sylvie Oliveira Lanceros-Méndez, S. Barandiarán, J. M |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Díaz, E. Valle, M. B. Ribeiro, Sylvie Oliveira Lanceros-Méndez, S. Barandiarán, J. M |
dc.subject.por.fl_str_mv |
PCL magnetite scaffolds magnetism cytotoxicity in vitro degradation Ciências Naturais::Ciências Físicas Science & Technology |
topic |
PCL magnetite scaffolds magnetism cytotoxicity in vitro degradation Ciências Naturais::Ciências Físicas Science & Technology |
description |
A study of Magnetite (Fe3O4) as a suitable matrix for the improved adhesion and proliferation of MC3T3-E1 pre-osteoblast cells in bone regeneration is presented. Biodegradable and magnetic polycaprolactone (PCL)/magnetite (Fe3O4) scaffolds, which were fabricated by Thermally Induced Phase Separation, are likewise analyzed. Various techniques are used to investigate in vitro degradation at 37 °C, over 104 weeks, in a phosphate buffered saline (PBS) solution. Magnetic measurements that were performed at physiological temperature (310 K) indicated that degradation neither modified the nature nor the distribution of the magnetite nanoparticles. The coercive field strength of the porous matrices demonstrated ferromagnetic behavior and the probable presence of particle interactions. The added nanoparticles facilitated the absorption of PBS, with no considerable increase in matrix degradation rates, as shown by the Gel Permeation Chromatography (GPC) results for Mw, Mn, and I. There was no collapse of the scaffold structures that maintained their structural integrity. Their suitability for bone regeneration was also supported by the absence of matrix cytotoxicity in assays, even after additions of up to 20% magnetite. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/64609 |
url |
http://hdl.handle.net/1822/64609 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Díaz, E.; Valle, M.B.; Ribeiro, S.; Lanceros-Mendez, S.; Barandiarán, J.M. 3D Cytocompatible Composites of PCL/Magnetite. Materials 2019, 12, 3843. 1996-1944 10.3390/ma12233843 https://www.mdpi.com/1996-1944/12/23/3843 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132283910750208 |