Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/6422 |
Resumo: | The optimization of culture medium compositionwas carried out for improvement the recombinantcyprosin B production, an enzyme with high milk-clottingactivity. Response surface methodology (RSM) wasapplied to evaluate the effect of variables namely glucose,yeast extract (YE) and bactopeptone present in the culturemedium, used for recombinant cyprosin B production bytransformed Saccharomyces cerevisiae BJ1991 strain inshake-flask and bioreactor culture conditions. The centracomposite experimental design (CCD) was adopted toderive a statistical model for optimizing the composition ofthe fermentation medium. The optimal concentration estimatedfor each variable related to a theoretical maximumof cyprosin B activity (488 U mL-1) was 30 g L-1 glucose,15 g L-1 YE and 27 g L-1 bactopeptone. The optimizedmedium composition, based on empirical model, ledto a cyprosin B activity of 519 U mL-1, which correspondsto an increase of 46%. The fermentation using optimizedculture medium in a 5-L bioreactor allowed a significantincrease in biomass (82%) and recombinant cyprosin Bproduction (139%). The improvement in the recombinantcyprosin B production after optimization process can beconsidered adequate for large-scale applications, and theclotting activity of cyprosin B account for their use inindustrial cheese making. |
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Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese productionCulture media compositionCyprosin BFermentationResponse surface methodologySaccharomyces cerevisiaeThe optimization of culture medium compositionwas carried out for improvement the recombinantcyprosin B production, an enzyme with high milk-clottingactivity. Response surface methodology (RSM) wasapplied to evaluate the effect of variables namely glucose,yeast extract (YE) and bactopeptone present in the culturemedium, used for recombinant cyprosin B production bytransformed Saccharomyces cerevisiae BJ1991 strain inshake-flask and bioreactor culture conditions. The centracomposite experimental design (CCD) was adopted toderive a statistical model for optimizing the composition ofthe fermentation medium. The optimal concentration estimatedfor each variable related to a theoretical maximumof cyprosin B activity (488 U mL-1) was 30 g L-1 glucose,15 g L-1 YE and 27 g L-1 bactopeptone. The optimizedmedium composition, based on empirical model, ledto a cyprosin B activity of 519 U mL-1, which correspondsto an increase of 46%. The fermentation using optimizedculture medium in a 5-L bioreactor allowed a significantincrease in biomass (82%) and recombinant cyprosin Bproduction (139%). The improvement in the recombinantcyprosin B production after optimization process can beconsidered adequate for large-scale applications, and theclotting activity of cyprosin B account for their use inindustrial cheese making.Springer-VerlagVeritati - Repositório Institucional da Universidade Católica PortuguesaSampaio, Pedro N.Calado, Cecília R. C.Sousa, LiseteBressler, David C.Pais, Maria SaloméFonseca, Luís Pina2011-10-18T13:59:27Z20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/6422engSAMPAIO, Pedro N. [et al.] - Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production. European food research and technology [Em linha]. 231:2 (2010) 339-346. Disponível na Internet: http://www.springerlink.com/content/ar5871t50476k014 . ISSN 1438-2385.1438-238510.1007/s00217-010-1281-z1438-2385WOS:000277639000019info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:12:37Zoai:repositorio.ucp.pt:10400.14/6422Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:07:12.466576Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production |
title |
Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production |
spellingShingle |
Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production Sampaio, Pedro N. Culture media composition Cyprosin B Fermentation Response surface methodology Saccharomyces cerevisiae |
title_short |
Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production |
title_full |
Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production |
title_fullStr |
Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production |
title_full_unstemmed |
Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production |
title_sort |
Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production |
author |
Sampaio, Pedro N. |
author_facet |
Sampaio, Pedro N. Calado, Cecília R. C. Sousa, Lisete Bressler, David C. Pais, Maria Salomé Fonseca, Luís Pina |
author_role |
author |
author2 |
Calado, Cecília R. C. Sousa, Lisete Bressler, David C. Pais, Maria Salomé Fonseca, Luís Pina |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Sampaio, Pedro N. Calado, Cecília R. C. Sousa, Lisete Bressler, David C. Pais, Maria Salomé Fonseca, Luís Pina |
dc.subject.por.fl_str_mv |
Culture media composition Cyprosin B Fermentation Response surface methodology Saccharomyces cerevisiae |
topic |
Culture media composition Cyprosin B Fermentation Response surface methodology Saccharomyces cerevisiae |
description |
The optimization of culture medium compositionwas carried out for improvement the recombinantcyprosin B production, an enzyme with high milk-clottingactivity. Response surface methodology (RSM) wasapplied to evaluate the effect of variables namely glucose,yeast extract (YE) and bactopeptone present in the culturemedium, used for recombinant cyprosin B production bytransformed Saccharomyces cerevisiae BJ1991 strain inshake-flask and bioreactor culture conditions. The centracomposite experimental design (CCD) was adopted toderive a statistical model for optimizing the composition ofthe fermentation medium. The optimal concentration estimatedfor each variable related to a theoretical maximumof cyprosin B activity (488 U mL-1) was 30 g L-1 glucose,15 g L-1 YE and 27 g L-1 bactopeptone. The optimizedmedium composition, based on empirical model, ledto a cyprosin B activity of 519 U mL-1, which correspondsto an increase of 46%. The fermentation using optimizedculture medium in a 5-L bioreactor allowed a significantincrease in biomass (82%) and recombinant cyprosin Bproduction (139%). The improvement in the recombinantcyprosin B production after optimization process can beconsidered adequate for large-scale applications, and theclotting activity of cyprosin B account for their use inindustrial cheese making. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2010-01-01T00:00:00Z 2011-10-18T13:59:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/6422 |
url |
http://hdl.handle.net/10400.14/6422 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
SAMPAIO, Pedro N. [et al.] - Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production. European food research and technology [Em linha]. 231:2 (2010) 339-346. Disponível na Internet: http://www.springerlink.com/content/ar5871t50476k014 . ISSN 1438-2385. 1438-2385 10.1007/s00217-010-1281-z 1438-2385 WOS:000277639000019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer-Verlag |
publisher.none.fl_str_mv |
Springer-Verlag |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131740378234880 |