Ohmic heating as a new tool for protein scaffold engineering

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Rui Miguel Martins
Data de Publicação: 2021
Outros Autores: Pereira, Ricardo Nuno Correia, Vicente, A. A., Cavaco-Paulo, Artur, Ribeiro, Artur
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/70470
Resumo: Ohmic heating (OH) is recognised as an emerging processing technology which recently is gaining increasing attention due to its ability to induce and control protein functionality. In this study, OH was used for the first time in the production of scaffolds for tissue engineering. BSA/casein solutions were processed by OH, promoting protein denaturation and aggregation, followed by cold-gelation through the addition of Ca2+. The formation of stable scaffolds was mostly dependent on the temperature and treatment time during OH processing. The variations of the electric field (EF) induced changes in the functional properties of both gel forming solutions and final scaffolds (contact angle, swelling, porosity, compressive modulus and degradation rate). The scaffolds biological performance was evaluated regarding their ability to support the adhesion and proliferation of human fibroblast cells. The production process resulted in a non-cytotoxic material and the changes imposed by the presence of the EF during the scaffolds production improved cellular proliferation and metabolic activity. Protein functionalization assisted by OH presents a promising new alternative for the production of improved and tuneable protein-based scaffolds for tissue engineering.
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spelling Ohmic heating as a new tool for protein scaffold engineeringgelationscaffoldselectric filedtissue engineeringcell proliferationScience & TechnologyOhmic heating (OH) is recognised as an emerging processing technology which recently is gaining increasing attention due to its ability to induce and control protein functionality. In this study, OH was used for the first time in the production of scaffolds for tissue engineering. BSA/casein solutions were processed by OH, promoting protein denaturation and aggregation, followed by cold-gelation through the addition of Ca2+. The formation of stable scaffolds was mostly dependent on the temperature and treatment time during OH processing. The variations of the electric field (EF) induced changes in the functional properties of both gel forming solutions and final scaffolds (contact angle, swelling, porosity, compressive modulus and degradation rate). The scaffolds biological performance was evaluated regarding their ability to support the adhesion and proliferation of human fibroblast cells. The production process resulted in a non-cytotoxic material and the changes imposed by the presence of the EF during the scaffolds production improved cellular proliferation and metabolic activity. Protein functionalization assisted by OH presents a promising new alternative for the production of improved and tuneable protein-based scaffolds for tissue engineering.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoRodrigues, Rui Miguel MartinsPereira, Ricardo Nuno CorreiaVicente, A. A.Cavaco-Paulo, ArturRibeiro, Artur20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/70470engRodrigues, Rui M.; Pereira, Ricardo N.; Vicente, António A.; Cavaco-Paulo, Artur; Ribeiro, Artur, Ohmic heating as a new tool for protein scaffold engineering. Materials Science and Engineering C-Materials for Biological Applications, 120(111784), 20210928-493110.1016/j.msec.2020.11178433545911111784https://www.sciencedirect.com/science/article/abs/pii/S0928493120337036info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:42:28Zoai:repositorium.sdum.uminho.pt:1822/70470Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:39:42.976587Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Ohmic heating as a new tool for protein scaffold engineering
title Ohmic heating as a new tool for protein scaffold engineering
spellingShingle Ohmic heating as a new tool for protein scaffold engineering
Rodrigues, Rui Miguel Martins
gelation
scaffolds
electric filed
tissue engineering
cell proliferation
Science & Technology
title_short Ohmic heating as a new tool for protein scaffold engineering
title_full Ohmic heating as a new tool for protein scaffold engineering
title_fullStr Ohmic heating as a new tool for protein scaffold engineering
title_full_unstemmed Ohmic heating as a new tool for protein scaffold engineering
title_sort Ohmic heating as a new tool for protein scaffold engineering
author Rodrigues, Rui Miguel Martins
author_facet Rodrigues, Rui Miguel Martins
Pereira, Ricardo Nuno Correia
Vicente, A. A.
Cavaco-Paulo, Artur
Ribeiro, Artur
author_role author
author2 Pereira, Ricardo Nuno Correia
Vicente, A. A.
Cavaco-Paulo, Artur
Ribeiro, Artur
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Rodrigues, Rui Miguel Martins
Pereira, Ricardo Nuno Correia
Vicente, A. A.
Cavaco-Paulo, Artur
Ribeiro, Artur
dc.subject.por.fl_str_mv gelation
scaffolds
electric filed
tissue engineering
cell proliferation
Science & Technology
topic gelation
scaffolds
electric filed
tissue engineering
cell proliferation
Science & Technology
description Ohmic heating (OH) is recognised as an emerging processing technology which recently is gaining increasing attention due to its ability to induce and control protein functionality. In this study, OH was used for the first time in the production of scaffolds for tissue engineering. BSA/casein solutions were processed by OH, promoting protein denaturation and aggregation, followed by cold-gelation through the addition of Ca2+. The formation of stable scaffolds was mostly dependent on the temperature and treatment time during OH processing. The variations of the electric field (EF) induced changes in the functional properties of both gel forming solutions and final scaffolds (contact angle, swelling, porosity, compressive modulus and degradation rate). The scaffolds biological performance was evaluated regarding their ability to support the adhesion and proliferation of human fibroblast cells. The production process resulted in a non-cytotoxic material and the changes imposed by the presence of the EF during the scaffolds production improved cellular proliferation and metabolic activity. Protein functionalization assisted by OH presents a promising new alternative for the production of improved and tuneable protein-based scaffolds for tissue engineering.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/70470
url http://hdl.handle.net/1822/70470
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Rodrigues, Rui M.; Pereira, Ricardo N.; Vicente, António A.; Cavaco-Paulo, Artur; Ribeiro, Artur, Ohmic heating as a new tool for protein scaffold engineering. Materials Science and Engineering C-Materials for Biological Applications, 120(111784), 2021
0928-4931
10.1016/j.msec.2020.111784
33545911
111784
https://www.sciencedirect.com/science/article/abs/pii/S0928493120337036
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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