In vitro macrophage nitric oxide production by Pterospartum tridentatum (L.) Willk. inflorescence polysaccharides

Detalhes bibliográficos
Autor(a) principal: Martins, Vitor Manuel Ramalheira
Data de Publicação: 2017
Outros Autores: Simões, Joana A., Ferreira, Isabel C.F.R., Cruz, Maria T., Domingues, Maria R.M., Coimbra, Manuel A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/15308
Resumo: Pterospartum tridentatum (L.) Willk. decoctions of dried inflorescences are used in Portugal due to their claimed beneficial properties for various health disorders. To disclose the potential contribution of its polysaccharides to health benefits, in this work, hot water extracts from P. tridentatum inflorescences were prepared and fractionated by ethanol precipitation and anion exchange chromatography. The fraction rich in acetylated galactomannans evidenced an increase in nitric oxide (NO) production by macrophages. This activity decreased 60–75% after saponification, confirming that acetylation is an important structural feature for this biological property. In addition, the treatment of pectic polysaccharides with endo-polygalacturonase showed that type-I and type-II arabinogalactans, as well as low molecular weight galacturonans and xyloglucans, may also contribute to macrophage NO production. Thus, the polysaccharides present in P. tridentatum dried inflorescences may contribute to the health beneficial properties frequently attributed to the decoctions of this plant.
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spelling In vitro macrophage nitric oxide production by Pterospartum tridentatum (L.) Willk. inflorescence polysaccharidesAcetylated galactomannanMass spectrometryNitric oxideP. tridentatumPectic polysaccharidesPterospartum tridentatum (L.) Willk. decoctions of dried inflorescences are used in Portugal due to their claimed beneficial properties for various health disorders. To disclose the potential contribution of its polysaccharides to health benefits, in this work, hot water extracts from P. tridentatum inflorescences were prepared and fractionated by ethanol precipitation and anion exchange chromatography. The fraction rich in acetylated galactomannans evidenced an increase in nitric oxide (NO) production by macrophages. This activity decreased 60–75% after saponification, confirming that acetylation is an important structural feature for this biological property. In addition, the treatment of pectic polysaccharides with endo-polygalacturonase showed that type-I and type-II arabinogalactans, as well as low molecular weight galacturonans and xyloglucans, may also contribute to macrophage NO production. Thus, the polysaccharides present in P. tridentatum dried inflorescences may contribute to the health beneficial properties frequently attributed to the decoctions of this plant.Thanks are due to Fundac¸ ão para a Ciência e a Tecnologia(FCT, Portugal), European Union, QREN, FEDER, and COMPETEfor funding the QOPNA (Project PEst-C/QUI/UI0062/2013; FCOMP-01-0124-FEDER-037296), CNC (Project UID/NEU/04539/2013), andCIMO Research Units, and RNEM (REDE/1504/REM/2005 thatconcerns the Portuguese Mass Spectrometry Network). Thanksare also due to FCT for the PhD Grant of Vitor Martins(SFRH/PROTEC/49249/2008) and to Dr. Ana Maria Carvalho, fromCIMO, for the identification of the plant material.Biblioteca Digital do IPBMartins, Vitor Manuel RamalheiraSimões, Joana A.Ferreira, Isabel C.F.R.Cruz, Maria T.Domingues, Maria R.M.Coimbra, Manuel A.2018-01-25T10:00:00Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/15308engMartins, Vitor M.R.; Simões, Joana; Ferreira, Isabel; Cruz, Maria Teresa; Domingues, M. Rosário; Coimbra, Manuel A. (2017). In vitro macrophage nitric oxide production by Pterospartum tridentatum (L.) Willk. inflorescence polysaccharides. Carbohydrate Polymers. ISSN 0144-8617. 157, p. 176-1840144-861710.1016/j.carbpol.2016.09.079info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:35:48Zoai:bibliotecadigital.ipb.pt:10198/15308Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:04:55.969619Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In vitro macrophage nitric oxide production by Pterospartum tridentatum (L.) Willk. inflorescence polysaccharides
title In vitro macrophage nitric oxide production by Pterospartum tridentatum (L.) Willk. inflorescence polysaccharides
spellingShingle In vitro macrophage nitric oxide production by Pterospartum tridentatum (L.) Willk. inflorescence polysaccharides
Martins, Vitor Manuel Ramalheira
Acetylated galactomannan
Mass spectrometry
Nitric oxide
P. tridentatum
Pectic polysaccharides
title_short In vitro macrophage nitric oxide production by Pterospartum tridentatum (L.) Willk. inflorescence polysaccharides
title_full In vitro macrophage nitric oxide production by Pterospartum tridentatum (L.) Willk. inflorescence polysaccharides
title_fullStr In vitro macrophage nitric oxide production by Pterospartum tridentatum (L.) Willk. inflorescence polysaccharides
title_full_unstemmed In vitro macrophage nitric oxide production by Pterospartum tridentatum (L.) Willk. inflorescence polysaccharides
title_sort In vitro macrophage nitric oxide production by Pterospartum tridentatum (L.) Willk. inflorescence polysaccharides
author Martins, Vitor Manuel Ramalheira
author_facet Martins, Vitor Manuel Ramalheira
Simões, Joana A.
Ferreira, Isabel C.F.R.
Cruz, Maria T.
Domingues, Maria R.M.
Coimbra, Manuel A.
author_role author
author2 Simões, Joana A.
Ferreira, Isabel C.F.R.
Cruz, Maria T.
Domingues, Maria R.M.
Coimbra, Manuel A.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Martins, Vitor Manuel Ramalheira
Simões, Joana A.
Ferreira, Isabel C.F.R.
Cruz, Maria T.
Domingues, Maria R.M.
Coimbra, Manuel A.
dc.subject.por.fl_str_mv Acetylated galactomannan
Mass spectrometry
Nitric oxide
P. tridentatum
Pectic polysaccharides
topic Acetylated galactomannan
Mass spectrometry
Nitric oxide
P. tridentatum
Pectic polysaccharides
description Pterospartum tridentatum (L.) Willk. decoctions of dried inflorescences are used in Portugal due to their claimed beneficial properties for various health disorders. To disclose the potential contribution of its polysaccharides to health benefits, in this work, hot water extracts from P. tridentatum inflorescences were prepared and fractionated by ethanol precipitation and anion exchange chromatography. The fraction rich in acetylated galactomannans evidenced an increase in nitric oxide (NO) production by macrophages. This activity decreased 60–75% after saponification, confirming that acetylation is an important structural feature for this biological property. In addition, the treatment of pectic polysaccharides with endo-polygalacturonase showed that type-I and type-II arabinogalactans, as well as low molecular weight galacturonans and xyloglucans, may also contribute to macrophage NO production. Thus, the polysaccharides present in P. tridentatum dried inflorescences may contribute to the health beneficial properties frequently attributed to the decoctions of this plant.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
2018-01-25T10:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/15308
url http://hdl.handle.net/10198/15308
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Martins, Vitor M.R.; Simões, Joana; Ferreira, Isabel; Cruz, Maria Teresa; Domingues, M. Rosário; Coimbra, Manuel A. (2017). In vitro macrophage nitric oxide production by Pterospartum tridentatum (L.) Willk. inflorescence polysaccharides. Carbohydrate Polymers. ISSN 0144-8617. 157, p. 176-184
0144-8617
10.1016/j.carbpol.2016.09.079
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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