Separomics applied to the proteomics and peptidomics of low-abundance proteins: choice of methods and challenges - a review
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200009 |
Resumo: | The enrichment and isolation of proteins are considered limiting steps in proteomic studies. Identification of proteins whose expression is transient, those that are of low-abundance, and of natural peptides not described in databases, is still a great challenge. Plant extracts are in general complex, and contaminants interfere with the identification of proteins involved in important physiological processes, such as plant defense against pathogens. This review discusses the challenges and strategies of separomics applied to the identification of low-abundance proteins and peptides in plants, especially in plants challenged by pathogens. Separomics is described as a group of methodological strategies for the separation of protein molecules for proteomics. Several tools have been used to remove highly abundant proteins from samples and also non-protein contaminants. The use of chromatographic techniques, the partition of the proteome into subproteomes, and an effort to isolate proteins in their native form have allowed the isolation and identification of rare proteins involved in different processes. |
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Separomics applied to the proteomics and peptidomics of low-abundance proteins: choice of methods and challenges - a reviewsample preparationcomplex protein extractsubproteomeslow-abundance proteinscell wall proteinsThe enrichment and isolation of proteins are considered limiting steps in proteomic studies. Identification of proteins whose expression is transient, those that are of low-abundance, and of natural peptides not described in databases, is still a great challenge. Plant extracts are in general complex, and contaminants interfere with the identification of proteins involved in important physiological processes, such as plant defense against pathogens. This review discusses the challenges and strategies of separomics applied to the identification of low-abundance proteins and peptides in plants, especially in plants challenged by pathogens. Separomics is described as a group of methodological strategies for the separation of protein molecules for proteomics. Several tools have been used to remove highly abundant proteins from samples and also non-protein contaminants. The use of chromatographic techniques, the partition of the proteome into subproteomes, and an effort to isolate proteins in their native form have allowed the isolation and identification of rare proteins involved in different processes.Sociedade Brasileira de Genética2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200009Genetics and Molecular Biology v.35 n.1 suppl.1 2012reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572012000200009info:eu-repo/semantics/openAccessBaracat-Pereira,Maria CristinaBarbosa,Meire de OliveiraMagalhães Júnior,Marcos JorgeCarrijo,Lanna CliciaGames,Patrícia DiasAlmeida,Hebréia OliveiraSena Netto,José FabianoPereira,Matheus RodriguesBarros,Everaldo Gonçalves deeng2012-06-01T00:00:00Zoai:scielo:S1415-47572012000200009Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2012-06-01T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Separomics applied to the proteomics and peptidomics of low-abundance proteins: choice of methods and challenges - a review |
title |
Separomics applied to the proteomics and peptidomics of low-abundance proteins: choice of methods and challenges - a review |
spellingShingle |
Separomics applied to the proteomics and peptidomics of low-abundance proteins: choice of methods and challenges - a review Baracat-Pereira,Maria Cristina sample preparation complex protein extract subproteomes low-abundance proteins cell wall proteins |
title_short |
Separomics applied to the proteomics and peptidomics of low-abundance proteins: choice of methods and challenges - a review |
title_full |
Separomics applied to the proteomics and peptidomics of low-abundance proteins: choice of methods and challenges - a review |
title_fullStr |
Separomics applied to the proteomics and peptidomics of low-abundance proteins: choice of methods and challenges - a review |
title_full_unstemmed |
Separomics applied to the proteomics and peptidomics of low-abundance proteins: choice of methods and challenges - a review |
title_sort |
Separomics applied to the proteomics and peptidomics of low-abundance proteins: choice of methods and challenges - a review |
author |
Baracat-Pereira,Maria Cristina |
author_facet |
Baracat-Pereira,Maria Cristina Barbosa,Meire de Oliveira Magalhães Júnior,Marcos Jorge Carrijo,Lanna Clicia Games,Patrícia Dias Almeida,Hebréia Oliveira Sena Netto,José Fabiano Pereira,Matheus Rodrigues Barros,Everaldo Gonçalves de |
author_role |
author |
author2 |
Barbosa,Meire de Oliveira Magalhães Júnior,Marcos Jorge Carrijo,Lanna Clicia Games,Patrícia Dias Almeida,Hebréia Oliveira Sena Netto,José Fabiano Pereira,Matheus Rodrigues Barros,Everaldo Gonçalves de |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Baracat-Pereira,Maria Cristina Barbosa,Meire de Oliveira Magalhães Júnior,Marcos Jorge Carrijo,Lanna Clicia Games,Patrícia Dias Almeida,Hebréia Oliveira Sena Netto,José Fabiano Pereira,Matheus Rodrigues Barros,Everaldo Gonçalves de |
dc.subject.por.fl_str_mv |
sample preparation complex protein extract subproteomes low-abundance proteins cell wall proteins |
topic |
sample preparation complex protein extract subproteomes low-abundance proteins cell wall proteins |
description |
The enrichment and isolation of proteins are considered limiting steps in proteomic studies. Identification of proteins whose expression is transient, those that are of low-abundance, and of natural peptides not described in databases, is still a great challenge. Plant extracts are in general complex, and contaminants interfere with the identification of proteins involved in important physiological processes, such as plant defense against pathogens. This review discusses the challenges and strategies of separomics applied to the identification of low-abundance proteins and peptides in plants, especially in plants challenged by pathogens. Separomics is described as a group of methodological strategies for the separation of protein molecules for proteomics. Several tools have been used to remove highly abundant proteins from samples and also non-protein contaminants. The use of chromatographic techniques, the partition of the proteome into subproteomes, and an effort to isolate proteins in their native form have allowed the isolation and identification of rare proteins involved in different processes. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572012000200009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.35 n.1 suppl.1 2012 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122384558587904 |