Rapid quantitative turbidimetric spot test analysis of potassium in blood serum
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532004000500004 |
Resumo: | A spot test quantitative turbidimetric method was developed for the determination of potassium in blood serum. The precipitation of potassium is done in basic medium with the classical analytical reagent sodium tetraphenylborate. The measurement of the turbidity is directly performed in this solution without further dilution using a micro-turbidimetric cell developed in this work. Blood serum was mixed with the buffer and the analytical reagent solution. The turbidity is measured in a spectrophotometer at 700 nm. The calibration curve is a straight line in the working concentration range, presenting a correlation coefficient equal to 0.9998. The analytical results obtained with this method were compared with those obtained through the use of a potassium ion selective electrode. Complete agreement was achieved between the two methods at the 99% confidence level. The observed mean RSD was 3.8%. |
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Rapid quantitative turbidimetric spot test analysis of potassium in blood serumpotassiumserumtetraphenylborateturbidimetricmicro analysisspot testA spot test quantitative turbidimetric method was developed for the determination of potassium in blood serum. The precipitation of potassium is done in basic medium with the classical analytical reagent sodium tetraphenylborate. The measurement of the turbidity is directly performed in this solution without further dilution using a micro-turbidimetric cell developed in this work. Blood serum was mixed with the buffer and the analytical reagent solution. The turbidity is measured in a spectrophotometer at 700 nm. The calibration curve is a straight line in the working concentration range, presenting a correlation coefficient equal to 0.9998. The analytical results obtained with this method were compared with those obtained through the use of a potassium ion selective electrode. Complete agreement was achieved between the two methods at the 99% confidence level. The observed mean RSD was 3.8%.Sociedade Brasileira de Química2004-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532004000500004Journal of the Brazilian Chemical Society v.15 n.5 2004reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532004000500004info:eu-repo/semantics/openAccessTubino,MatthieuSouza,Rafael L. deHoehr,Nelci F.eng2004-11-10T00:00:00Zoai:scielo:S0103-50532004000500004Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2004-11-10T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Rapid quantitative turbidimetric spot test analysis of potassium in blood serum |
title |
Rapid quantitative turbidimetric spot test analysis of potassium in blood serum |
spellingShingle |
Rapid quantitative turbidimetric spot test analysis of potassium in blood serum Tubino,Matthieu potassium serum tetraphenylborate turbidimetric micro analysis spot test |
title_short |
Rapid quantitative turbidimetric spot test analysis of potassium in blood serum |
title_full |
Rapid quantitative turbidimetric spot test analysis of potassium in blood serum |
title_fullStr |
Rapid quantitative turbidimetric spot test analysis of potassium in blood serum |
title_full_unstemmed |
Rapid quantitative turbidimetric spot test analysis of potassium in blood serum |
title_sort |
Rapid quantitative turbidimetric spot test analysis of potassium in blood serum |
author |
Tubino,Matthieu |
author_facet |
Tubino,Matthieu Souza,Rafael L. de Hoehr,Nelci F. |
author_role |
author |
author2 |
Souza,Rafael L. de Hoehr,Nelci F. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Tubino,Matthieu Souza,Rafael L. de Hoehr,Nelci F. |
dc.subject.por.fl_str_mv |
potassium serum tetraphenylborate turbidimetric micro analysis spot test |
topic |
potassium serum tetraphenylborate turbidimetric micro analysis spot test |
description |
A spot test quantitative turbidimetric method was developed for the determination of potassium in blood serum. The precipitation of potassium is done in basic medium with the classical analytical reagent sodium tetraphenylborate. The measurement of the turbidity is directly performed in this solution without further dilution using a micro-turbidimetric cell developed in this work. Blood serum was mixed with the buffer and the analytical reagent solution. The turbidity is measured in a spectrophotometer at 700 nm. The calibration curve is a straight line in the working concentration range, presenting a correlation coefficient equal to 0.9998. The analytical results obtained with this method were compared with those obtained through the use of a potassium ion selective electrode. Complete agreement was achieved between the two methods at the 99% confidence level. The observed mean RSD was 3.8%. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532004000500004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532004000500004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532004000500004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.15 n.5 2004 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318165782429696 |