Rapid quantitative turbidimetric spot test analysis of potassium in blood serum

Detalhes bibliográficos
Autor(a) principal: Tubino,Matthieu
Data de Publicação: 2004
Outros Autores: Souza,Rafael L. de, Hoehr,Nelci F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532004000500004
Resumo: A spot test quantitative turbidimetric method was developed for the determination of potassium in blood serum. The precipitation of potassium is done in basic medium with the classical analytical reagent sodium tetraphenylborate. The measurement of the turbidity is directly performed in this solution without further dilution using a micro-turbidimetric cell developed in this work. Blood serum was mixed with the buffer and the analytical reagent solution. The turbidity is measured in a spectrophotometer at 700 nm. The calibration curve is a straight line in the working concentration range, presenting a correlation coefficient equal to 0.9998. The analytical results obtained with this method were compared with those obtained through the use of a potassium ion selective electrode. Complete agreement was achieved between the two methods at the 99% confidence level. The observed mean RSD was 3.8%.
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spelling Rapid quantitative turbidimetric spot test analysis of potassium in blood serumpotassiumserumtetraphenylborateturbidimetricmicro analysisspot testA spot test quantitative turbidimetric method was developed for the determination of potassium in blood serum. The precipitation of potassium is done in basic medium with the classical analytical reagent sodium tetraphenylborate. The measurement of the turbidity is directly performed in this solution without further dilution using a micro-turbidimetric cell developed in this work. Blood serum was mixed with the buffer and the analytical reagent solution. The turbidity is measured in a spectrophotometer at 700 nm. The calibration curve is a straight line in the working concentration range, presenting a correlation coefficient equal to 0.9998. The analytical results obtained with this method were compared with those obtained through the use of a potassium ion selective electrode. Complete agreement was achieved between the two methods at the 99% confidence level. The observed mean RSD was 3.8%.Sociedade Brasileira de Química2004-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532004000500004Journal of the Brazilian Chemical Society v.15 n.5 2004reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532004000500004info:eu-repo/semantics/openAccessTubino,MatthieuSouza,Rafael L. deHoehr,Nelci F.eng2004-11-10T00:00:00Zoai:scielo:S0103-50532004000500004Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2004-11-10T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Rapid quantitative turbidimetric spot test analysis of potassium in blood serum
title Rapid quantitative turbidimetric spot test analysis of potassium in blood serum
spellingShingle Rapid quantitative turbidimetric spot test analysis of potassium in blood serum
Tubino,Matthieu
potassium
serum
tetraphenylborate
turbidimetric
micro analysis
spot test
title_short Rapid quantitative turbidimetric spot test analysis of potassium in blood serum
title_full Rapid quantitative turbidimetric spot test analysis of potassium in blood serum
title_fullStr Rapid quantitative turbidimetric spot test analysis of potassium in blood serum
title_full_unstemmed Rapid quantitative turbidimetric spot test analysis of potassium in blood serum
title_sort Rapid quantitative turbidimetric spot test analysis of potassium in blood serum
author Tubino,Matthieu
author_facet Tubino,Matthieu
Souza,Rafael L. de
Hoehr,Nelci F.
author_role author
author2 Souza,Rafael L. de
Hoehr,Nelci F.
author2_role author
author
dc.contributor.author.fl_str_mv Tubino,Matthieu
Souza,Rafael L. de
Hoehr,Nelci F.
dc.subject.por.fl_str_mv potassium
serum
tetraphenylborate
turbidimetric
micro analysis
spot test
topic potassium
serum
tetraphenylborate
turbidimetric
micro analysis
spot test
description A spot test quantitative turbidimetric method was developed for the determination of potassium in blood serum. The precipitation of potassium is done in basic medium with the classical analytical reagent sodium tetraphenylborate. The measurement of the turbidity is directly performed in this solution without further dilution using a micro-turbidimetric cell developed in this work. Blood serum was mixed with the buffer and the analytical reagent solution. The turbidity is measured in a spectrophotometer at 700 nm. The calibration curve is a straight line in the working concentration range, presenting a correlation coefficient equal to 0.9998. The analytical results obtained with this method were compared with those obtained through the use of a potassium ion selective electrode. Complete agreement was achieved between the two methods at the 99% confidence level. The observed mean RSD was 3.8%.
publishDate 2004
dc.date.none.fl_str_mv 2004-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532004000500004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532004000500004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532004000500004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.15 n.5 2004
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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