Micropropagation of Alibertia edulis Rich
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600004 |
Resumo: | The aim of this work was to develop a micropropagation protocol for Alibertia edulis, a medicinal Brazilian Cerrado plant. A. edulis seeds were sterilized and germinated in water-agar. Fifteen millimeter long explants from the seedlings cultivated for 50 days were used for the multiplication in three consecutive subcultures. The cytokinin benzyladenine (BA) alone or combined with indole-3-butyric acid (IBA) was added to ¼ MS medium. The treatments containing combinations of the BA + IBA induced more shoot proliferation than the BA alone, with the optimum concentration of 0.5 mg.L-1 for both growth regulators. A. edulis shoots elongated easily and the presence of growth regulators did have a positive effect on the shoot elongation. Consecutive subcultures also had a positive effect on both sprouting and shoot elongation. Activated charcoal was essential for rooting, and all rooted plants were able to acclimatize. |
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Brazilian Archives of Biology and Technology |
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Micropropagation of Alibertia edulis RichCerradoAlibertia edulisrootinggerminationactivated charcoalThe aim of this work was to develop a micropropagation protocol for Alibertia edulis, a medicinal Brazilian Cerrado plant. A. edulis seeds were sterilized and germinated in water-agar. Fifteen millimeter long explants from the seedlings cultivated for 50 days were used for the multiplication in three consecutive subcultures. The cytokinin benzyladenine (BA) alone or combined with indole-3-butyric acid (IBA) was added to ¼ MS medium. The treatments containing combinations of the BA + IBA induced more shoot proliferation than the BA alone, with the optimum concentration of 0.5 mg.L-1 for both growth regulators. A. edulis shoots elongated easily and the presence of growth regulators did have a positive effect on the shoot elongation. Consecutive subcultures also had a positive effect on both sprouting and shoot elongation. Activated charcoal was essential for rooting, and all rooted plants were able to acclimatize.Instituto de Tecnologia do Paraná - Tecpar2008-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600004Brazilian Archives of Biology and Technology v.51 n.6 2008reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132008000600004info:eu-repo/semantics/openAccessSilva,Fernanda Augusta Boato daPereira,Luiz Alfredo RodriguesSilveira,Conceição Eneida dos Santoseng2009-01-27T00:00:00Zoai:scielo:S1516-89132008000600004Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2009-01-27T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Micropropagation of Alibertia edulis Rich |
title |
Micropropagation of Alibertia edulis Rich |
spellingShingle |
Micropropagation of Alibertia edulis Rich Silva,Fernanda Augusta Boato da Cerrado Alibertia edulis rooting germination activated charcoal |
title_short |
Micropropagation of Alibertia edulis Rich |
title_full |
Micropropagation of Alibertia edulis Rich |
title_fullStr |
Micropropagation of Alibertia edulis Rich |
title_full_unstemmed |
Micropropagation of Alibertia edulis Rich |
title_sort |
Micropropagation of Alibertia edulis Rich |
author |
Silva,Fernanda Augusta Boato da |
author_facet |
Silva,Fernanda Augusta Boato da Pereira,Luiz Alfredo Rodrigues Silveira,Conceição Eneida dos Santos |
author_role |
author |
author2 |
Pereira,Luiz Alfredo Rodrigues Silveira,Conceição Eneida dos Santos |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Silva,Fernanda Augusta Boato da Pereira,Luiz Alfredo Rodrigues Silveira,Conceição Eneida dos Santos |
dc.subject.por.fl_str_mv |
Cerrado Alibertia edulis rooting germination activated charcoal |
topic |
Cerrado Alibertia edulis rooting germination activated charcoal |
description |
The aim of this work was to develop a micropropagation protocol for Alibertia edulis, a medicinal Brazilian Cerrado plant. A. edulis seeds were sterilized and germinated in water-agar. Fifteen millimeter long explants from the seedlings cultivated for 50 days were used for the multiplication in three consecutive subcultures. The cytokinin benzyladenine (BA) alone or combined with indole-3-butyric acid (IBA) was added to ¼ MS medium. The treatments containing combinations of the BA + IBA induced more shoot proliferation than the BA alone, with the optimum concentration of 0.5 mg.L-1 for both growth regulators. A. edulis shoots elongated easily and the presence of growth regulators did have a positive effect on the shoot elongation. Consecutive subcultures also had a positive effect on both sprouting and shoot elongation. Activated charcoal was essential for rooting, and all rooted plants were able to acclimatize. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132008000600004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.51 n.6 2008 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318272350257152 |