Differential Expression of Human MicroRNAs During Dengue Virus Infection in THP-1 Monocytes
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.3389/fcimb.2021.714088 http://hdl.handle.net/1843/56493 https://orcid.org/0000-0001-6235-8807 https://orcid.org/0000-0001-5180-3717 https://orcid.org/0000-0003-2622-534X https://orcid.org/0000-0002-7660-7058 https://orcid.org/0000-0002-8663-3364 https://orcid.org/0000-0002-3969-704X https://orcid.org/0000-0003-0570-750X |
Resumo: | Dengue virus (DENV) is the most widespread arbovirus, responsible for a wide range of clinical manifestations, varying from self-limited illness to severe hemorrhagic fever. Dengue severity is associated with host intense proinflammatory response and monocytes have been considered one of the key cell types involved in the early steps of DENV infection and immunopathogenesis. To better understand cellular mechanisms involved in monocyte infection by DENV, we analyzed the expression levels of 754 human microRNAs in DENV-infected THP-1 cells, a human monocytic cell line. Eleven human microRNAs showed differential expression after DENV infection and gene ontology and enrichment analysis revealed biological processes potentially affected by these molecules. Five downregulated microRNAs were significantly linked to cellular response to stress, four to cell death/apoptosis, two to innate immune responses and one upregulated to vesicle mediated, TGF-β signaling, phosphatidylinositol mediated signaling, lipid metabolism process and blood coagulation. |
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2023-07-17T20:12:38Z2023-07-17T20:12:38Z20211118https://doi.org/10.3389/fcimb.2021.7140882235-2988http://hdl.handle.net/1843/56493https://orcid.org/0000-0001-6235-8807https://orcid.org/0000-0001-5180-3717https://orcid.org/0000-0003-2622-534Xhttps://orcid.org/0000-0002-7660-7058https://orcid.org/0000-0002-8663-3364https://orcid.org/0000-0002-3969-704Xhttps://orcid.org/0000-0003-0570-750XDengue virus (DENV) is the most widespread arbovirus, responsible for a wide range of clinical manifestations, varying from self-limited illness to severe hemorrhagic fever. Dengue severity is associated with host intense proinflammatory response and monocytes have been considered one of the key cell types involved in the early steps of DENV infection and immunopathogenesis. To better understand cellular mechanisms involved in monocyte infection by DENV, we analyzed the expression levels of 754 human microRNAs in DENV-infected THP-1 cells, a human monocytic cell line. Eleven human microRNAs showed differential expression after DENV infection and gene ontology and enrichment analysis revealed biological processes potentially affected by these molecules. Five downregulated microRNAs were significantly linked to cellular response to stress, four to cell death/apoptosis, two to innate immune responses and one upregulated to vesicle mediated, TGF-β signaling, phosphatidylinositol mediated signaling, lipid metabolism process and blood coagulation.porUniversidade Federal de Minas GeraisUFMGBrasilICB - INSTITUTO DE CIÊNCIAS BIOLOGICASFrontiers in Cellular and Infection MicrobiologyDengue - EpidemiologiaRelação hospedeiro-virusMonocitosGenetica - ExpressãoMicroRNAMonocyteDengueGene expressionVirus-hostDifferential Expression of Human MicroRNAs During Dengue Virus Infection in THP-1 Monocytesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.frontiersin.org/articles/10.3389/fcimb.2021.714088/fullÁtila Duque RossiRenato Santana de AguiarLuiza Mendonça HigaAlice Laschuk HerlingerMarcelo Ribeiro AlvesMariane Talon de MenezesAna Lucia Moraes GianniniCynthia Chester CardosoAndrea da PoianAmilcar Tanuriinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/56493/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALDifferential Expression of Human MicroRNAs During Dengue Virus Infection in THP-1 Monocytes.pdfDifferential Expression of Human MicroRNAs During Dengue Virus Infection in THP-1 Monocytes.pdfapplication/pdf355246https://repositorio.ufmg.br/bitstream/1843/56493/2/Differential%20Expression%20of%20Human%20MicroRNAs%20During%20Dengue%20Virus%20Infection%20in%20THP-1%20Monocytes.pdfc53760365f3663f415b2009ba540ce4fMD521843/564932023-07-17 17:12:38.932oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-07-17T20:12:38Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Differential Expression of Human MicroRNAs During Dengue Virus Infection in THP-1 Monocytes |
title |
Differential Expression of Human MicroRNAs During Dengue Virus Infection in THP-1 Monocytes |
spellingShingle |
Differential Expression of Human MicroRNAs During Dengue Virus Infection in THP-1 Monocytes Átila Duque Rossi MicroRNA Monocyte Dengue Gene expression Virus-host Dengue - Epidemiologia Relação hospedeiro-virus Monocitos Genetica - Expressão |
title_short |
Differential Expression of Human MicroRNAs During Dengue Virus Infection in THP-1 Monocytes |
title_full |
Differential Expression of Human MicroRNAs During Dengue Virus Infection in THP-1 Monocytes |
title_fullStr |
Differential Expression of Human MicroRNAs During Dengue Virus Infection in THP-1 Monocytes |
title_full_unstemmed |
Differential Expression of Human MicroRNAs During Dengue Virus Infection in THP-1 Monocytes |
title_sort |
Differential Expression of Human MicroRNAs During Dengue Virus Infection in THP-1 Monocytes |
author |
Átila Duque Rossi |
author_facet |
Átila Duque Rossi Renato Santana de Aguiar Luiza Mendonça Higa Alice Laschuk Herlinger Marcelo Ribeiro Alves Mariane Talon de Menezes Ana Lucia Moraes Giannini Cynthia Chester Cardoso Andrea da Poian Amilcar Tanuri |
author_role |
author |
author2 |
Renato Santana de Aguiar Luiza Mendonça Higa Alice Laschuk Herlinger Marcelo Ribeiro Alves Mariane Talon de Menezes Ana Lucia Moraes Giannini Cynthia Chester Cardoso Andrea da Poian Amilcar Tanuri |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Átila Duque Rossi Renato Santana de Aguiar Luiza Mendonça Higa Alice Laschuk Herlinger Marcelo Ribeiro Alves Mariane Talon de Menezes Ana Lucia Moraes Giannini Cynthia Chester Cardoso Andrea da Poian Amilcar Tanuri |
dc.subject.por.fl_str_mv |
MicroRNA Monocyte Dengue Gene expression Virus-host |
topic |
MicroRNA Monocyte Dengue Gene expression Virus-host Dengue - Epidemiologia Relação hospedeiro-virus Monocitos Genetica - Expressão |
dc.subject.other.pt_BR.fl_str_mv |
Dengue - Epidemiologia Relação hospedeiro-virus Monocitos Genetica - Expressão |
description |
Dengue virus (DENV) is the most widespread arbovirus, responsible for a wide range of clinical manifestations, varying from self-limited illness to severe hemorrhagic fever. Dengue severity is associated with host intense proinflammatory response and monocytes have been considered one of the key cell types involved in the early steps of DENV infection and immunopathogenesis. To better understand cellular mechanisms involved in monocyte infection by DENV, we analyzed the expression levels of 754 human microRNAs in DENV-infected THP-1 cells, a human monocytic cell line. Eleven human microRNAs showed differential expression after DENV infection and gene ontology and enrichment analysis revealed biological processes potentially affected by these molecules. Five downregulated microRNAs were significantly linked to cellular response to stress, four to cell death/apoptosis, two to innate immune responses and one upregulated to vesicle mediated, TGF-β signaling, phosphatidylinositol mediated signaling, lipid metabolism process and blood coagulation. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021 |
dc.date.accessioned.fl_str_mv |
2023-07-17T20:12:38Z |
dc.date.available.fl_str_mv |
2023-07-17T20:12:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/56493 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.3389/fcimb.2021.714088 |
dc.identifier.issn.pt_BR.fl_str_mv |
2235-2988 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0001-6235-8807 https://orcid.org/0000-0001-5180-3717 https://orcid.org/0000-0003-2622-534X https://orcid.org/0000-0002-7660-7058 https://orcid.org/0000-0002-8663-3364 https://orcid.org/0000-0002-3969-704X https://orcid.org/0000-0003-0570-750X |
url |
https://doi.org/10.3389/fcimb.2021.714088 http://hdl.handle.net/1843/56493 https://orcid.org/0000-0001-6235-8807 https://orcid.org/0000-0001-5180-3717 https://orcid.org/0000-0003-2622-534X https://orcid.org/0000-0002-7660-7058 https://orcid.org/0000-0002-8663-3364 https://orcid.org/0000-0002-3969-704X https://orcid.org/0000-0003-0570-750X |
identifier_str_mv |
2235-2988 |
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por |
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por |
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Frontiers in Cellular and Infection Microbiology |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ICB - INSTITUTO DE CIÊNCIAS BIOLOGICAS |
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Universidade Federal de Minas Gerais |
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