Vaccination of C57BL/10 mice against cutaneous leishmaniasis using killed promastigotes of different strains and species of Leishmania.

Detalhes bibliográficos
Autor(a) principal: Mayrink, Wilson
Data de Publicação: 2002
Outros Autores: Santos, Gilmara Cristina dos, Toledo, Vicente de Paulo Coelho Peixoto de, Guimarães, Tânia Mara Pinto Dabés, Coelho, George Luiz Lins Machado, Genaro, Odair, Costa, Carlos Alberto da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFOP
Texto Completo: http://www.repositorio.ufop.br/handle/123456789/3764
http://dx.doi.org/10.1590/S0037-86822002000200001
Resumo: Antigenic extracts from five Leishmania stocks were used to vaccinate C57BL/10 mice. The Leishvacinand PH8 monovalent vaccine yielded the highest IFN-levels in the supernatants of spleen cell culture from vaccinated animals. Each single strain immunized group showed evidence of protective immunity six months after the challenge with promastigotes of Leishmania (Leishmania) amazonensis. No differences were detected between the vaccinated groups. It can be concluded that vaccines composed of single Leishmania stocks can provide protection to C57BL/10 mice against L. (L.) amazonensis infection.
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spelling Mayrink, WilsonSantos, Gilmara Cristina dosToledo, Vicente de Paulo Coelho Peixoto deGuimarães, Tânia Mara Pinto DabésCoelho, George Luiz Lins MachadoGenaro, OdairCosta, Carlos Alberto da2014-11-11T17:27:10Z2014-11-11T17:27:10Z2002MAYRINK, W. Vaccination of C57BL/10 mice against cutaneous leishmaniasis using killed promastigotes of different strains and species of Leishmania. Revista do Instituto de Medicina Tropical de São Paulo, São Paulo, v. 35, n. 2, p. 125-132, 2002. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822002000200001&lng=en&nrm=iso&tlng=en>. Acesso em: 01 set. 2014.0036-4665http://www.repositorio.ufop.br/handle/123456789/3764http://dx.doi.org/10.1590/S0037-86822002000200001Antigenic extracts from five Leishmania stocks were used to vaccinate C57BL/10 mice. The Leishvacinand PH8 monovalent vaccine yielded the highest IFN-levels in the supernatants of spleen cell culture from vaccinated animals. Each single strain immunized group showed evidence of protective immunity six months after the challenge with promastigotes of Leishmania (Leishmania) amazonensis. No differences were detected between the vaccinated groups. It can be concluded that vaccines composed of single Leishmania stocks can provide protection to C57BL/10 mice against L. (L.) amazonensis infection.Estudos anteriores revelaram que uma vacina preparada com promastigotas mortas de cinco cepas de Leishmania pode induzir uma imunidade protetora para a leishmaniose tegumentar americana no homem e em modelos experimentais. Um dos problemas do uso desta vacina é a complexidade de sua composição e a necessidade de se incorporar diferentes cepas de Leishmania. Por esta razão, extratos antigênicos de cada uma das cinco cepas constituintes da vacina foram preparados e usados individualmente em estudos imunológicos com camundongos C57BL/10. A Leishvacine a vacina monovalente PH8 induziram os maiores níveis de Interferon-g (IFN-) detectado no sobrenadante de células esplênicas dos animais vacinados. Cada grupo imunizado com vacinas monovalentes desenvolveram uma imunidade protetora seis meses após a infecção desafio com promastigotas de Leishmania (Leishmania) amazonensis e nenhuma diferença estatística foi observada entre os grupos vacinados. Pode-se concluir que vacinas compostas por cepas isoladas de Leishmania protegem camundongos C57BL/10 contra, pelo menos, da infecção por L. (L.) amazonensis.VaccineInterferonInterleukinLeishmaniaVacinaVaccination of C57BL/10 mice against cutaneous leishmaniasis using killed promastigotes of different strains and species of Leishmania.Vacinação de camundongos C57BL/10 contra leishmaniose com promastigotas mortas de diferentes cepas e espécies de Leishmania.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleA Revista do Instituto de Medicina tropical de São Paulo permite que o Repositório Institucional da Universidade Federal de Ouro Preto (UFOP) deposite uma cópia eletrônica dos artigos publicados por esse periódico  em que ao menos um dos autores faça parte da comunidade cientifica da UFOP. Fonte: Licença concedida mediante preenchimento de formulário em 06/12/2013.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://www.repositorio.ufop.br/bitstream/123456789/3764/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52ORIGINALARTIGO_VaccinationC57BLMice.pdfARTIGO_VaccinationC57BLMice.pdfapplication/pdf140425http://www.repositorio.ufop.br/bitstream/123456789/3764/1/ARTIGO_VaccinationC57BLMice.pdf763fb49a3e2a47a4f4ad8a7a23490d00MD51123456789/37642019-05-06 14:14:04.626oai:localhost: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Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-05-06T18:14:04Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.pt_BR.fl_str_mv Vaccination of C57BL/10 mice against cutaneous leishmaniasis using killed promastigotes of different strains and species of Leishmania.
dc.title.alternative.pt_BR.fl_str_mv Vacinação de camundongos C57BL/10 contra leishmaniose com promastigotas mortas de diferentes cepas e espécies de Leishmania.
title Vaccination of C57BL/10 mice against cutaneous leishmaniasis using killed promastigotes of different strains and species of Leishmania.
spellingShingle Vaccination of C57BL/10 mice against cutaneous leishmaniasis using killed promastigotes of different strains and species of Leishmania.
Mayrink, Wilson
Vaccine
Interferon
Interleukin
Leishmania
Vacina
title_short Vaccination of C57BL/10 mice against cutaneous leishmaniasis using killed promastigotes of different strains and species of Leishmania.
title_full Vaccination of C57BL/10 mice against cutaneous leishmaniasis using killed promastigotes of different strains and species of Leishmania.
title_fullStr Vaccination of C57BL/10 mice against cutaneous leishmaniasis using killed promastigotes of different strains and species of Leishmania.
title_full_unstemmed Vaccination of C57BL/10 mice against cutaneous leishmaniasis using killed promastigotes of different strains and species of Leishmania.
title_sort Vaccination of C57BL/10 mice against cutaneous leishmaniasis using killed promastigotes of different strains and species of Leishmania.
author Mayrink, Wilson
author_facet Mayrink, Wilson
Santos, Gilmara Cristina dos
Toledo, Vicente de Paulo Coelho Peixoto de
Guimarães, Tânia Mara Pinto Dabés
Coelho, George Luiz Lins Machado
Genaro, Odair
Costa, Carlos Alberto da
author_role author
author2 Santos, Gilmara Cristina dos
Toledo, Vicente de Paulo Coelho Peixoto de
Guimarães, Tânia Mara Pinto Dabés
Coelho, George Luiz Lins Machado
Genaro, Odair
Costa, Carlos Alberto da
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mayrink, Wilson
Santos, Gilmara Cristina dos
Toledo, Vicente de Paulo Coelho Peixoto de
Guimarães, Tânia Mara Pinto Dabés
Coelho, George Luiz Lins Machado
Genaro, Odair
Costa, Carlos Alberto da
dc.subject.por.fl_str_mv Vaccine
Interferon
Interleukin
Leishmania
Vacina
topic Vaccine
Interferon
Interleukin
Leishmania
Vacina
description Antigenic extracts from five Leishmania stocks were used to vaccinate C57BL/10 mice. The Leishvacinand PH8 monovalent vaccine yielded the highest IFN-levels in the supernatants of spleen cell culture from vaccinated animals. Each single strain immunized group showed evidence of protective immunity six months after the challenge with promastigotes of Leishmania (Leishmania) amazonensis. No differences were detected between the vaccinated groups. It can be concluded that vaccines composed of single Leishmania stocks can provide protection to C57BL/10 mice against L. (L.) amazonensis infection.
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2014-11-11T17:27:10Z
dc.date.available.fl_str_mv 2014-11-11T17:27:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv MAYRINK, W. Vaccination of C57BL/10 mice against cutaneous leishmaniasis using killed promastigotes of different strains and species of Leishmania. Revista do Instituto de Medicina Tropical de São Paulo, São Paulo, v. 35, n. 2, p. 125-132, 2002. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822002000200001&lng=en&nrm=iso&tlng=en>. Acesso em: 01 set. 2014.
dc.identifier.uri.fl_str_mv http://www.repositorio.ufop.br/handle/123456789/3764
dc.identifier.issn.none.fl_str_mv 0036-4665
dc.identifier.doi.none.fl_str_mv http://dx.doi.org/10.1590/S0037-86822002000200001
identifier_str_mv MAYRINK, W. Vaccination of C57BL/10 mice against cutaneous leishmaniasis using killed promastigotes of different strains and species of Leishmania. Revista do Instituto de Medicina Tropical de São Paulo, São Paulo, v. 35, n. 2, p. 125-132, 2002. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822002000200001&lng=en&nrm=iso&tlng=en>. Acesso em: 01 set. 2014.
0036-4665
url http://www.repositorio.ufop.br/handle/123456789/3764
http://dx.doi.org/10.1590/S0037-86822002000200001
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