Naringin : antitumor potential in silico and in vitro on bladder cancer cells.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/jspui/handle/123456789/15943 https://doi.org/10.30827/ars.v63i2.22430 |
Resumo: | Introduction: Urothelial carcinoma is a significant public health problem. Transitional cell carcinoma (TCC) is the most common subtype, accounting for approximately 90 % of all bladder cancers. Chemotherapeutic protocols have been studied, but some present high toxicity and low tolerability. Naringin is a polyphenolic compound found mainly in citrus fruits, which antitumor activity has been studied in several types of cancer. However, there is little information about naringin effects on bladder cancer. This study aimed to evaluate the antitumor potential of nar- ingin in silico and in vitro using two bladder cancer cell lines Method: In silico analysis was carried out by PASS Online software. In vitro, the effects of naringin treatment (12.5 - 400 μM) were evaluated regarding its cytotoxicity, clonogenic survival, morphological alterations, cell cycle pro- gression, migration, and mutagenicity Results: In silico analyses predicted antitumor activity through several mechanisms of action. In vitro results showed naringin presented cytotoxic effects, reduced the number of colonies, inhibited cell migration, and changed the morphology and cell cycle progression of the two cell lines evaluated. However, naringin did not present mu- tagenic effects. Conclusions: Naringin has antiproliferative activity and is a promising candidate for bladder cancer treatment. |
id |
UFOP_b658e2ee5a4505704dea56bea041126c |
---|---|
oai_identifier_str |
oai:repositorio.ufop.br:123456789/15943 |
network_acronym_str |
UFOP |
network_name_str |
Repositório Institucional da UFOP |
repository_id_str |
3233 |
spelling |
Naringin : antitumor potential in silico and in vitro on bladder cancer cells.Naringina : potencial antitumoral in silico e in vitro en células cancerosas de vejiga.Cell cycle progressionFlavonoidProgresión del ciclo celularIntroduction: Urothelial carcinoma is a significant public health problem. Transitional cell carcinoma (TCC) is the most common subtype, accounting for approximately 90 % of all bladder cancers. Chemotherapeutic protocols have been studied, but some present high toxicity and low tolerability. Naringin is a polyphenolic compound found mainly in citrus fruits, which antitumor activity has been studied in several types of cancer. However, there is little information about naringin effects on bladder cancer. This study aimed to evaluate the antitumor potential of nar- ingin in silico and in vitro using two bladder cancer cell lines Method: In silico analysis was carried out by PASS Online software. In vitro, the effects of naringin treatment (12.5 - 400 μM) were evaluated regarding its cytotoxicity, clonogenic survival, morphological alterations, cell cycle pro- gression, migration, and mutagenicity Results: In silico analyses predicted antitumor activity through several mechanisms of action. In vitro results showed naringin presented cytotoxic effects, reduced the number of colonies, inhibited cell migration, and changed the morphology and cell cycle progression of the two cell lines evaluated. However, naringin did not present mu- tagenic effects. Conclusions: Naringin has antiproliferative activity and is a promising candidate for bladder cancer treatment.Introducción: el carcinoma urotelial es un problema de salud pública importante. El carcinoma de células de tran- sición es el subtipo más común y representa aproximadamente el 90 % de todos los cánceres de vejiga. Se han estudiado protocolos quimioterapéuticos, pero algunos presentan alta toxicidad y baja tolerabilidad. La naringina es un compuesto polifenólico que se encuentra principalmente en los cítricos, cuya actividad antitumoral se ha estudiado en varios tipos de cáncer. Sin embargo, hay poca información sobre los efectos de la naringina en el cáncer de vejiga. Este estudio tuvo como objetivo evaluar el potencial antitumoral de la naringina in silico e in vitro utilizando dos líneas celulares de cáncer de vejiga. Método: el análisis in silico se llevó a cabo mediante el software PASS Online. In vitro, se evaluaron los efectos del tratamiento con naringina (12,5 - 400 μM) en cuanto a su citotoxicidad, supervivencia clonogénica, alteraciones morfológicas, progresión del ciclo celular, migración y mutagenicidad. Resultados: los análisis in silico predijeron la actividad antitumoral a través de varios mecanismos de acción. Los resultados in vitro mostraron que la naringina presentó efectos citotóxicos, redujo el número de colonias, inhibió la migración celular y cambió la morfología y la progresión del ciclo celular de las dos líneas celulares evaluadas. Sin embargo, la naringina no presentó efectos mutagénicos. Conclusiones: la naringina tiene actividad antiproliferativa y es un candidato prometedor para el tratamiento del cáncer de vejiga.2023-01-16T21:09:32Z2023-01-16T21:09:32Z2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfRADICCHI, D. C. et al. Naringin: antitumor potential in silico and in vitro on bladder cancer cells. Ars Pharmaceutica, v. 63, p. 132-143, 2022. Disponível em: <https://revistaseug.ugr.es/index.php/ars/article/view/22430>. Acesso em: 11 out. 2022.2340-9894http://www.repositorio.ufop.br/jspui/handle/123456789/15943https://doi.org/10.30827/ars.v63i2.22430This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Fonte: o PDF do artigo.info:eu-repo/semantics/openAccessRadicchi, Débora CarvalhoMelo, André SacramentoLima, Ana Paula BragaAlmeida, Tamires CunhaSouza, Gustavo Henrique Bianco deSilva, Glenda Nicioli daengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2023-01-16T21:09:35Zoai:repositorio.ufop.br:123456789/15943Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332023-01-16T21:09:35Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.none.fl_str_mv |
Naringin : antitumor potential in silico and in vitro on bladder cancer cells. Naringina : potencial antitumoral in silico e in vitro en células cancerosas de vejiga. |
title |
Naringin : antitumor potential in silico and in vitro on bladder cancer cells. |
spellingShingle |
Naringin : antitumor potential in silico and in vitro on bladder cancer cells. Radicchi, Débora Carvalho Cell cycle progression Flavonoid Progresión del ciclo celular |
title_short |
Naringin : antitumor potential in silico and in vitro on bladder cancer cells. |
title_full |
Naringin : antitumor potential in silico and in vitro on bladder cancer cells. |
title_fullStr |
Naringin : antitumor potential in silico and in vitro on bladder cancer cells. |
title_full_unstemmed |
Naringin : antitumor potential in silico and in vitro on bladder cancer cells. |
title_sort |
Naringin : antitumor potential in silico and in vitro on bladder cancer cells. |
author |
Radicchi, Débora Carvalho |
author_facet |
Radicchi, Débora Carvalho Melo, André Sacramento Lima, Ana Paula Braga Almeida, Tamires Cunha Souza, Gustavo Henrique Bianco de Silva, Glenda Nicioli da |
author_role |
author |
author2 |
Melo, André Sacramento Lima, Ana Paula Braga Almeida, Tamires Cunha Souza, Gustavo Henrique Bianco de Silva, Glenda Nicioli da |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Radicchi, Débora Carvalho Melo, André Sacramento Lima, Ana Paula Braga Almeida, Tamires Cunha Souza, Gustavo Henrique Bianco de Silva, Glenda Nicioli da |
dc.subject.por.fl_str_mv |
Cell cycle progression Flavonoid Progresión del ciclo celular |
topic |
Cell cycle progression Flavonoid Progresión del ciclo celular |
description |
Introduction: Urothelial carcinoma is a significant public health problem. Transitional cell carcinoma (TCC) is the most common subtype, accounting for approximately 90 % of all bladder cancers. Chemotherapeutic protocols have been studied, but some present high toxicity and low tolerability. Naringin is a polyphenolic compound found mainly in citrus fruits, which antitumor activity has been studied in several types of cancer. However, there is little information about naringin effects on bladder cancer. This study aimed to evaluate the antitumor potential of nar- ingin in silico and in vitro using two bladder cancer cell lines Method: In silico analysis was carried out by PASS Online software. In vitro, the effects of naringin treatment (12.5 - 400 μM) were evaluated regarding its cytotoxicity, clonogenic survival, morphological alterations, cell cycle pro- gression, migration, and mutagenicity Results: In silico analyses predicted antitumor activity through several mechanisms of action. In vitro results showed naringin presented cytotoxic effects, reduced the number of colonies, inhibited cell migration, and changed the morphology and cell cycle progression of the two cell lines evaluated. However, naringin did not present mu- tagenic effects. Conclusions: Naringin has antiproliferative activity and is a promising candidate for bladder cancer treatment. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 2023-01-16T21:09:32Z 2023-01-16T21:09:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
RADICCHI, D. C. et al. Naringin: antitumor potential in silico and in vitro on bladder cancer cells. Ars Pharmaceutica, v. 63, p. 132-143, 2022. Disponível em: <https://revistaseug.ugr.es/index.php/ars/article/view/22430>. Acesso em: 11 out. 2022. 2340-9894 http://www.repositorio.ufop.br/jspui/handle/123456789/15943 https://doi.org/10.30827/ars.v63i2.22430 |
identifier_str_mv |
RADICCHI, D. C. et al. Naringin: antitumor potential in silico and in vitro on bladder cancer cells. Ars Pharmaceutica, v. 63, p. 132-143, 2022. Disponível em: <https://revistaseug.ugr.es/index.php/ars/article/view/22430>. Acesso em: 11 out. 2022. 2340-9894 |
url |
http://www.repositorio.ufop.br/jspui/handle/123456789/15943 https://doi.org/10.30827/ars.v63i2.22430 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
instname_str |
Universidade Federal de Ouro Preto (UFOP) |
instacron_str |
UFOP |
institution |
UFOP |
reponame_str |
Repositório Institucional da UFOP |
collection |
Repositório Institucional da UFOP |
repository.name.fl_str_mv |
Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP) |
repository.mail.fl_str_mv |
repositorio@ufop.edu.br |
_version_ |
1813002813312073728 |