Naringin : antitumor potential in silico and in vitro on bladder cancer cells.

Detalhes bibliográficos
Autor(a) principal: Radicchi, Débora Carvalho
Data de Publicação: 2022
Outros Autores: Melo, André Sacramento, Lima, Ana Paula Braga, Almeida, Tamires Cunha, Souza, Gustavo Henrique Bianco de, Silva, Glenda Nicioli da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFOP
Texto Completo: http://www.repositorio.ufop.br/jspui/handle/123456789/15943
https://doi.org/10.30827/ars.v63i2.22430
Resumo: Introduction: Urothelial carcinoma is a significant public health problem. Transitional cell carcinoma (TCC) is the most common subtype, accounting for approximately 90 % of all bladder cancers. Chemotherapeutic protocols have been studied, but some present high toxicity and low tolerability. Naringin is a polyphenolic compound found mainly in citrus fruits, which antitumor activity has been studied in several types of cancer. However, there is little information about naringin effects on bladder cancer. This study aimed to evaluate the antitumor potential of nar- ingin in silico and in vitro using two bladder cancer cell lines Method: In silico analysis was carried out by PASS Online software. In vitro, the effects of naringin treatment (12.5 - 400 μM) were evaluated regarding its cytotoxicity, clonogenic survival, morphological alterations, cell cycle pro- gression, migration, and mutagenicity Results: In silico analyses predicted antitumor activity through several mechanisms of action. In vitro results showed naringin presented cytotoxic effects, reduced the number of colonies, inhibited cell migration, and changed the morphology and cell cycle progression of the two cell lines evaluated. However, naringin did not present mu- tagenic effects. Conclusions: Naringin has antiproliferative activity and is a promising candidate for bladder cancer treatment.
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spelling Naringin : antitumor potential in silico and in vitro on bladder cancer cells.Naringina : potencial antitumoral in silico e in vitro en células cancerosas de vejiga.Cell cycle progressionFlavonoidProgresión del ciclo celularIntroduction: Urothelial carcinoma is a significant public health problem. Transitional cell carcinoma (TCC) is the most common subtype, accounting for approximately 90 % of all bladder cancers. Chemotherapeutic protocols have been studied, but some present high toxicity and low tolerability. Naringin is a polyphenolic compound found mainly in citrus fruits, which antitumor activity has been studied in several types of cancer. However, there is little information about naringin effects on bladder cancer. This study aimed to evaluate the antitumor potential of nar- ingin in silico and in vitro using two bladder cancer cell lines Method: In silico analysis was carried out by PASS Online software. In vitro, the effects of naringin treatment (12.5 - 400 μM) were evaluated regarding its cytotoxicity, clonogenic survival, morphological alterations, cell cycle pro- gression, migration, and mutagenicity Results: In silico analyses predicted antitumor activity through several mechanisms of action. In vitro results showed naringin presented cytotoxic effects, reduced the number of colonies, inhibited cell migration, and changed the morphology and cell cycle progression of the two cell lines evaluated. However, naringin did not present mu- tagenic effects. Conclusions: Naringin has antiproliferative activity and is a promising candidate for bladder cancer treatment.Introducción: el carcinoma urotelial es un problema de salud pública importante. El carcinoma de células de tran- sición es el subtipo más común y representa aproximadamente el 90 % de todos los cánceres de vejiga. Se han estudiado protocolos quimioterapéuticos, pero algunos presentan alta toxicidad y baja tolerabilidad. La naringina es un compuesto polifenólico que se encuentra principalmente en los cítricos, cuya actividad antitumoral se ha estudiado en varios tipos de cáncer. Sin embargo, hay poca información sobre los efectos de la naringina en el cáncer de vejiga. Este estudio tuvo como objetivo evaluar el potencial antitumoral de la naringina in silico e in vitro utilizando dos líneas celulares de cáncer de vejiga. Método: el análisis in silico se llevó a cabo mediante el software PASS Online. In vitro, se evaluaron los efectos del tratamiento con naringina (12,5 - 400 μM) en cuanto a su citotoxicidad, supervivencia clonogénica, alteraciones morfológicas, progresión del ciclo celular, migración y mutagenicidad. Resultados: los análisis in silico predijeron la actividad antitumoral a través de varios mecanismos de acción. Los resultados in vitro mostraron que la naringina presentó efectos citotóxicos, redujo el número de colonias, inhibió la migración celular y cambió la morfología y la progresión del ciclo celular de las dos líneas celulares evaluadas. Sin embargo, la naringina no presentó efectos mutagénicos. Conclusiones: la naringina tiene actividad antiproliferativa y es un candidato prometedor para el tratamiento del cáncer de vejiga.2023-01-16T21:09:32Z2023-01-16T21:09:32Z2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfRADICCHI, D. C. et al. Naringin: antitumor potential in silico and in vitro on bladder cancer cells. Ars Pharmaceutica, v. 63, p. 132-143, 2022. Disponível em: <https://revistaseug.ugr.es/index.php/ars/article/view/22430>. Acesso em: 11 out. 2022.2340-9894http://www.repositorio.ufop.br/jspui/handle/123456789/15943https://doi.org/10.30827/ars.v63i2.22430This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Fonte: o PDF do artigo.info:eu-repo/semantics/openAccessRadicchi, Débora CarvalhoMelo, André SacramentoLima, Ana Paula BragaAlmeida, Tamires CunhaSouza, Gustavo Henrique Bianco deSilva, Glenda Nicioli daengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2023-01-16T21:09:35Zoai:repositorio.ufop.br:123456789/15943Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332023-01-16T21:09:35Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.none.fl_str_mv Naringin : antitumor potential in silico and in vitro on bladder cancer cells.
Naringina : potencial antitumoral in silico e in vitro en células cancerosas de vejiga.
title Naringin : antitumor potential in silico and in vitro on bladder cancer cells.
spellingShingle Naringin : antitumor potential in silico and in vitro on bladder cancer cells.
Radicchi, Débora Carvalho
Cell cycle progression
Flavonoid
Progresión del ciclo celular
title_short Naringin : antitumor potential in silico and in vitro on bladder cancer cells.
title_full Naringin : antitumor potential in silico and in vitro on bladder cancer cells.
title_fullStr Naringin : antitumor potential in silico and in vitro on bladder cancer cells.
title_full_unstemmed Naringin : antitumor potential in silico and in vitro on bladder cancer cells.
title_sort Naringin : antitumor potential in silico and in vitro on bladder cancer cells.
author Radicchi, Débora Carvalho
author_facet Radicchi, Débora Carvalho
Melo, André Sacramento
Lima, Ana Paula Braga
Almeida, Tamires Cunha
Souza, Gustavo Henrique Bianco de
Silva, Glenda Nicioli da
author_role author
author2 Melo, André Sacramento
Lima, Ana Paula Braga
Almeida, Tamires Cunha
Souza, Gustavo Henrique Bianco de
Silva, Glenda Nicioli da
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Radicchi, Débora Carvalho
Melo, André Sacramento
Lima, Ana Paula Braga
Almeida, Tamires Cunha
Souza, Gustavo Henrique Bianco de
Silva, Glenda Nicioli da
dc.subject.por.fl_str_mv Cell cycle progression
Flavonoid
Progresión del ciclo celular
topic Cell cycle progression
Flavonoid
Progresión del ciclo celular
description Introduction: Urothelial carcinoma is a significant public health problem. Transitional cell carcinoma (TCC) is the most common subtype, accounting for approximately 90 % of all bladder cancers. Chemotherapeutic protocols have been studied, but some present high toxicity and low tolerability. Naringin is a polyphenolic compound found mainly in citrus fruits, which antitumor activity has been studied in several types of cancer. However, there is little information about naringin effects on bladder cancer. This study aimed to evaluate the antitumor potential of nar- ingin in silico and in vitro using two bladder cancer cell lines Method: In silico analysis was carried out by PASS Online software. In vitro, the effects of naringin treatment (12.5 - 400 μM) were evaluated regarding its cytotoxicity, clonogenic survival, morphological alterations, cell cycle pro- gression, migration, and mutagenicity Results: In silico analyses predicted antitumor activity through several mechanisms of action. In vitro results showed naringin presented cytotoxic effects, reduced the number of colonies, inhibited cell migration, and changed the morphology and cell cycle progression of the two cell lines evaluated. However, naringin did not present mu- tagenic effects. Conclusions: Naringin has antiproliferative activity and is a promising candidate for bladder cancer treatment.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023-01-16T21:09:32Z
2023-01-16T21:09:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv RADICCHI, D. C. et al. Naringin: antitumor potential in silico and in vitro on bladder cancer cells. Ars Pharmaceutica, v. 63, p. 132-143, 2022. Disponível em: <https://revistaseug.ugr.es/index.php/ars/article/view/22430>. Acesso em: 11 out. 2022.
2340-9894
http://www.repositorio.ufop.br/jspui/handle/123456789/15943
https://doi.org/10.30827/ars.v63i2.22430
identifier_str_mv RADICCHI, D. C. et al. Naringin: antitumor potential in silico and in vitro on bladder cancer cells. Ars Pharmaceutica, v. 63, p. 132-143, 2022. Disponível em: <https://revistaseug.ugr.es/index.php/ars/article/view/22430>. Acesso em: 11 out. 2022.
2340-9894
url http://www.repositorio.ufop.br/jspui/handle/123456789/15943
https://doi.org/10.30827/ars.v63i2.22430
dc.language.iso.fl_str_mv eng
language eng
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFOP
instname:Universidade Federal de Ouro Preto (UFOP)
instacron:UFOP
instname_str Universidade Federal de Ouro Preto (UFOP)
instacron_str UFOP
institution UFOP
reponame_str Repositório Institucional da UFOP
collection Repositório Institucional da UFOP
repository.name.fl_str_mv Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)
repository.mail.fl_str_mv repositorio@ufop.edu.br
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