Aqueous two-phase systems applied to partition proteins from goat milk whey in-nature
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/32533 |
Resumo: | The proteins coming from the milk whey have numerous functional properties. Among the proteins with high bioactivity, α-lactoalbumin (α-La) and β-lactoglobulin (β-Lg) are present in large quantities in the milk whey. In the separation process of proteins, it is important to choose techniques which besides ensuring purity and high yield will not affect the molecule biological activity. The aqueous two-phase systems (ATS) have been utilized with success in the partition of these proteins, however, the studies were performed using protein in its pure form. Studies using milk whey in-nature and goat milk whey have not been found yet. In this context, the objective of this study was to evaluate the liquid liquid equilibrium of aqueous two-phase systems (ATS) in the partition of α-La and β-Lg from goat milk whey in-nature. Equilibrium data were performed considering ATS comprised of polyethylene glycol, potassium phosphate and water at 25˚C and pH 7.0. The influence of the polymer molecular weight and amount of goat milk whey in-nature on the partition coefficient of these proteins were assessed. The partition coefficient, selectivity, process yield and purity of α-lactoalbumin and β- actoglobulin proteins were determined. The results showed that the separation technique by aqueous biphasic systems is applicable indicating high efficiency in the whey proteins separation process |
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Freire, Luziany Adyja da CostaCourtial, Camila Gambini Pereira2021-05-17T14:36:39Z2021-05-17T14:36:39Z2016-09-13A. C. FREIRE, LUZIANY ; G. PEREIRA, CAMILA . Aqueous Two-Phase Systems Applied to Partition Proteins from Goat Milk Whey In-Nature. Advances in Bioscience and Biotechnology, v. 07, p. 350-359, 2016. Disponível em: https://www.scirp.org/journal/paperinformation.aspx?paperid=70531. Acesso em: 06 abr. 2021. http://dx.doi.org/10.4236/abb.2016.790342156-85022156-8456https://repositorio.ufrn.br/handle/123456789/3253310.4236/abb.2016.79034Scientific Research PublishingAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessGoat Milk Wheyα-Lactoalbuminβ-LactoglobulinAqueous Biphasic SystemsAqueous two-phase systems applied to partition proteins from goat milk whey in-natureinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThe proteins coming from the milk whey have numerous functional properties. Among the proteins with high bioactivity, α-lactoalbumin (α-La) and β-lactoglobulin (β-Lg) are present in large quantities in the milk whey. In the separation process of proteins, it is important to choose techniques which besides ensuring purity and high yield will not affect the molecule biological activity. The aqueous two-phase systems (ATS) have been utilized with success in the partition of these proteins, however, the studies were performed using protein in its pure form. Studies using milk whey in-nature and goat milk whey have not been found yet. In this context, the objective of this study was to evaluate the liquid liquid equilibrium of aqueous two-phase systems (ATS) in the partition of α-La and β-Lg from goat milk whey in-nature. Equilibrium data were performed considering ATS comprised of polyethylene glycol, potassium phosphate and water at 25˚C and pH 7.0. The influence of the polymer molecular weight and amount of goat milk whey in-nature on the partition coefficient of these proteins were assessed. The partition coefficient, selectivity, process yield and purity of α-lactoalbumin and β- actoglobulin proteins were determined. The results showed that the separation technique by aqueous biphasic systems is applicable indicating high efficiency in the whey proteins separation processengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNLICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/32533/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53ORIGINALAqueousTwo-Phase_PEREIRA_2016.pdfAqueousTwo-Phase_PEREIRA_2016.pdfArtigoapplication/pdf352193https://repositorio.ufrn.br/bitstream/123456789/32533/1/AqueousTwo-Phase_PEREIRA_2016.pdf8de4d3b8947624762fdb7e368f51317aMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/32533/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52123456789/325332021-05-17 11:36:39.683oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-05-17T14:36:39Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Aqueous two-phase systems applied to partition proteins from goat milk whey in-nature |
title |
Aqueous two-phase systems applied to partition proteins from goat milk whey in-nature |
spellingShingle |
Aqueous two-phase systems applied to partition proteins from goat milk whey in-nature Freire, Luziany Adyja da Costa Goat Milk Whey α-Lactoalbumin β-Lactoglobulin Aqueous Biphasic Systems |
title_short |
Aqueous two-phase systems applied to partition proteins from goat milk whey in-nature |
title_full |
Aqueous two-phase systems applied to partition proteins from goat milk whey in-nature |
title_fullStr |
Aqueous two-phase systems applied to partition proteins from goat milk whey in-nature |
title_full_unstemmed |
Aqueous two-phase systems applied to partition proteins from goat milk whey in-nature |
title_sort |
Aqueous two-phase systems applied to partition proteins from goat milk whey in-nature |
author |
Freire, Luziany Adyja da Costa |
author_facet |
Freire, Luziany Adyja da Costa Courtial, Camila Gambini Pereira |
author_role |
author |
author2 |
Courtial, Camila Gambini Pereira |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Freire, Luziany Adyja da Costa Courtial, Camila Gambini Pereira |
dc.subject.por.fl_str_mv |
Goat Milk Whey α-Lactoalbumin β-Lactoglobulin Aqueous Biphasic Systems |
topic |
Goat Milk Whey α-Lactoalbumin β-Lactoglobulin Aqueous Biphasic Systems |
description |
The proteins coming from the milk whey have numerous functional properties. Among the proteins with high bioactivity, α-lactoalbumin (α-La) and β-lactoglobulin (β-Lg) are present in large quantities in the milk whey. In the separation process of proteins, it is important to choose techniques which besides ensuring purity and high yield will not affect the molecule biological activity. The aqueous two-phase systems (ATS) have been utilized with success in the partition of these proteins, however, the studies were performed using protein in its pure form. Studies using milk whey in-nature and goat milk whey have not been found yet. In this context, the objective of this study was to evaluate the liquid liquid equilibrium of aqueous two-phase systems (ATS) in the partition of α-La and β-Lg from goat milk whey in-nature. Equilibrium data were performed considering ATS comprised of polyethylene glycol, potassium phosphate and water at 25˚C and pH 7.0. The influence of the polymer molecular weight and amount of goat milk whey in-nature on the partition coefficient of these proteins were assessed. The partition coefficient, selectivity, process yield and purity of α-lactoalbumin and β- actoglobulin proteins were determined. The results showed that the separation technique by aqueous biphasic systems is applicable indicating high efficiency in the whey proteins separation process |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-09-13 |
dc.date.accessioned.fl_str_mv |
2021-05-17T14:36:39Z |
dc.date.available.fl_str_mv |
2021-05-17T14:36:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
A. C. FREIRE, LUZIANY ; G. PEREIRA, CAMILA . Aqueous Two-Phase Systems Applied to Partition Proteins from Goat Milk Whey In-Nature. Advances in Bioscience and Biotechnology, v. 07, p. 350-359, 2016. Disponível em: https://www.scirp.org/journal/paperinformation.aspx?paperid=70531. Acesso em: 06 abr. 2021. http://dx.doi.org/10.4236/abb.2016.79034 |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/32533 |
dc.identifier.issn.none.fl_str_mv |
2156-8502 2156-8456 |
dc.identifier.doi.none.fl_str_mv |
10.4236/abb.2016.79034 |
identifier_str_mv |
A. C. FREIRE, LUZIANY ; G. PEREIRA, CAMILA . Aqueous Two-Phase Systems Applied to Partition Proteins from Goat Milk Whey In-Nature. Advances in Bioscience and Biotechnology, v. 07, p. 350-359, 2016. Disponível em: https://www.scirp.org/journal/paperinformation.aspx?paperid=70531. Acesso em: 06 abr. 2021. http://dx.doi.org/10.4236/abb.2016.79034 2156-8502 2156-8456 10.4236/abb.2016.79034 |
url |
https://repositorio.ufrn.br/handle/123456789/32533 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ |
eu_rights_str_mv |
openAccess |
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Scientific Research Publishing |
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Scientific Research Publishing |
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