Análise morfológica dos folículos primordiais de ovários de ovino criopreservados em EG, DMSO e sua associaçã o
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.researchgate.net/profile/Bruno_Carvalho7/publication/235990150_Use_of_ovarian_reserve_markers_and_variables_of_response_to_gonadotropic_stimulus_as_predictors_of_embryo_implantation_in_ICSI_cycles/file/5046351545001f25bb.pdf http://hdl.handle.net/11449/71083 |
Resumo: | Objective: Compare the cryoprotectants Dimethyl Sulphoxide (DMSO), Ethylene Glycol (EG) and their association for cryopreservation of sheep ovarian cortex. Methodology: Fragments collected from ovaries were divided into 3 parts. 1. One part from sample was destined for analysis of fresh material. 2. The second part was incubated with solution of freezing having 1,5M EG or 1,5M DMSO or 1,5MEG + 1,5M DMSO and washed for dilution of the cryoprotectants. 3. The third part was submitted to cryopreservation using the same cryoprotectans (EG 1,5M; DMSO 1,5M and EG + DMSO 1,5M) and cryopreserved. In all groups, one part of sample was submitted to pre-antral follicles isolation and the remainder was destined to ultra-structural analysis. Results: After isolation of fresh primordial follicles (control), the percentage of viable follicles was 78,9%. The percentage of viable follicles only exposed to cryoprotectants 1,5M EG, 1,5M DMSO and 1,5M EG + 1,5M DMSO was 77,1%, 68,4% and 60,7% respectively. After cryopreservation were 75%, 60% and 55,6% respectively. Ultra-structural analysis of the primordial follicles derived from fresh ovarian fragments or from fragments just exposed to the cryoprotectants showed similar morphology. However, in frozen samples, alterations of mitochondria were observed in all groups. Despite this, the integrity of the remained organelles was preserved in follicles cryopreserved with EG, while that in others groups (DMSO and association) an excess of vacuolizaton in cytoplasm of oocytes and swelling of nuclear membrane was observed indicating degeneration. Conclusion: The Ehilene Glycol seems to be the cryoprotector more adequated for cryopreservation of sheep ovarian tissue. |
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Análise morfológica dos folículos primordiais de ovários de ovino criopreservados em EG, DMSO e sua associaçã oMorphological analysis of primordial follicles from sheep ovaries cryopreserved in EG, DMSO and their associationCryopreservationOvarian follicleTransmission electron microscopydimethyl sulfoxideethylene glycolanimal tissuecell degenerationcell isolationcell ultrastructurecell vacuolecell viabilitycomparative studycontrolled studycryopreservationcytotoxicityfreezingmitochondrionnonhumanovary folliclesheeptransmission electron microscopyObjective: Compare the cryoprotectants Dimethyl Sulphoxide (DMSO), Ethylene Glycol (EG) and their association for cryopreservation of sheep ovarian cortex. Methodology: Fragments collected from ovaries were divided into 3 parts. 1. One part from sample was destined for analysis of fresh material. 2. The second part was incubated with solution of freezing having 1,5M EG or 1,5M DMSO or 1,5MEG + 1,5M DMSO and washed for dilution of the cryoprotectants. 3. The third part was submitted to cryopreservation using the same cryoprotectans (EG 1,5M; DMSO 1,5M and EG + DMSO 1,5M) and cryopreserved. In all groups, one part of sample was submitted to pre-antral follicles isolation and the remainder was destined to ultra-structural analysis. Results: After isolation of fresh primordial follicles (control), the percentage of viable follicles was 78,9%. The percentage of viable follicles only exposed to cryoprotectants 1,5M EG, 1,5M DMSO and 1,5M EG + 1,5M DMSO was 77,1%, 68,4% and 60,7% respectively. After cryopreservation were 75%, 60% and 55,6% respectively. Ultra-structural analysis of the primordial follicles derived from fresh ovarian fragments or from fragments just exposed to the cryoprotectants showed similar morphology. However, in frozen samples, alterations of mitochondria were observed in all groups. Despite this, the integrity of the remained organelles was preserved in follicles cryopreserved with EG, while that in others groups (DMSO and association) an excess of vacuolizaton in cytoplasm of oocytes and swelling of nuclear membrane was observed indicating degeneration. Conclusion: The Ehilene Glycol seems to be the cryoprotector more adequated for cryopreservation of sheep ovarian tissue.Embriologista da Clínica Endogin Serh, Bauru - SPDepartamento de Urologia Faculdade de Medicina da UNESP, Botucatu - SPDepartamento de Reprodução Animal Faculdade de Medicina Veterinãria e Zootécnica da UNESP, Botucatu - SPDepartamento de Urologia Faculdade de Medicina da UNESP, Botucatu - SPDepartamento de Reprodução Animal Faculdade de Medicina Veterinãria e Zootécnica da UNESP, Botucatu - SPEmbriologista da Clínica Endogin SerhUniversidade Estadual Paulista (Unesp)Tsuribe, Patrícia MiyukiGobbo, Carlos Alberto Monte [UNESP]Landim-Alvarenga, Fernanda da Cruz [UNESP]2014-05-27T11:23:56Z2014-05-27T11:23:56Z2009-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9-13http://www.researchgate.net/profile/Bruno_Carvalho7/publication/235990150_Use_of_ovarian_reserve_markers_and_variables_of_response_to_gonadotropic_stimulus_as_predictors_of_embryo_implantation_in_ICSI_cycles/file/5046351545001f25bb.pdfJornal Brasileiro de Reproducao Assistida, v. 13, n. 3, p. 9-13, 2009.1517-56931518-0557http://hdl.handle.net/11449/710832-s2.0-77649137095501759507224891784564903008148330000-0002-2420-2550Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporJornal Brasileiro de Reproducao Assistida0,282info:eu-repo/semantics/openAccess2024-09-09T14:01:30Zoai:repositorio.unesp.br:11449/71083Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:01:30Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Análise morfológica dos folículos primordiais de ovários de ovino criopreservados em EG, DMSO e sua associaçã o Morphological analysis of primordial follicles from sheep ovaries cryopreserved in EG, DMSO and their association |
title |
Análise morfológica dos folículos primordiais de ovários de ovino criopreservados em EG, DMSO e sua associaçã o |
spellingShingle |
Análise morfológica dos folículos primordiais de ovários de ovino criopreservados em EG, DMSO e sua associaçã o Tsuribe, Patrícia Miyuki Cryopreservation Ovarian follicle Transmission electron microscopy dimethyl sulfoxide ethylene glycol animal tissue cell degeneration cell isolation cell ultrastructure cell vacuole cell viability comparative study controlled study cryopreservation cytotoxicity freezing mitochondrion nonhuman ovary follicle sheep transmission electron microscopy |
title_short |
Análise morfológica dos folículos primordiais de ovários de ovino criopreservados em EG, DMSO e sua associaçã o |
title_full |
Análise morfológica dos folículos primordiais de ovários de ovino criopreservados em EG, DMSO e sua associaçã o |
title_fullStr |
Análise morfológica dos folículos primordiais de ovários de ovino criopreservados em EG, DMSO e sua associaçã o |
title_full_unstemmed |
Análise morfológica dos folículos primordiais de ovários de ovino criopreservados em EG, DMSO e sua associaçã o |
title_sort |
Análise morfológica dos folículos primordiais de ovários de ovino criopreservados em EG, DMSO e sua associaçã o |
author |
Tsuribe, Patrícia Miyuki |
author_facet |
Tsuribe, Patrícia Miyuki Gobbo, Carlos Alberto Monte [UNESP] Landim-Alvarenga, Fernanda da Cruz [UNESP] |
author_role |
author |
author2 |
Gobbo, Carlos Alberto Monte [UNESP] Landim-Alvarenga, Fernanda da Cruz [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Embriologista da Clínica Endogin Serh Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Tsuribe, Patrícia Miyuki Gobbo, Carlos Alberto Monte [UNESP] Landim-Alvarenga, Fernanda da Cruz [UNESP] |
dc.subject.por.fl_str_mv |
Cryopreservation Ovarian follicle Transmission electron microscopy dimethyl sulfoxide ethylene glycol animal tissue cell degeneration cell isolation cell ultrastructure cell vacuole cell viability comparative study controlled study cryopreservation cytotoxicity freezing mitochondrion nonhuman ovary follicle sheep transmission electron microscopy |
topic |
Cryopreservation Ovarian follicle Transmission electron microscopy dimethyl sulfoxide ethylene glycol animal tissue cell degeneration cell isolation cell ultrastructure cell vacuole cell viability comparative study controlled study cryopreservation cytotoxicity freezing mitochondrion nonhuman ovary follicle sheep transmission electron microscopy |
description |
Objective: Compare the cryoprotectants Dimethyl Sulphoxide (DMSO), Ethylene Glycol (EG) and their association for cryopreservation of sheep ovarian cortex. Methodology: Fragments collected from ovaries were divided into 3 parts. 1. One part from sample was destined for analysis of fresh material. 2. The second part was incubated with solution of freezing having 1,5M EG or 1,5M DMSO or 1,5MEG + 1,5M DMSO and washed for dilution of the cryoprotectants. 3. The third part was submitted to cryopreservation using the same cryoprotectans (EG 1,5M; DMSO 1,5M and EG + DMSO 1,5M) and cryopreserved. In all groups, one part of sample was submitted to pre-antral follicles isolation and the remainder was destined to ultra-structural analysis. Results: After isolation of fresh primordial follicles (control), the percentage of viable follicles was 78,9%. The percentage of viable follicles only exposed to cryoprotectants 1,5M EG, 1,5M DMSO and 1,5M EG + 1,5M DMSO was 77,1%, 68,4% and 60,7% respectively. After cryopreservation were 75%, 60% and 55,6% respectively. Ultra-structural analysis of the primordial follicles derived from fresh ovarian fragments or from fragments just exposed to the cryoprotectants showed similar morphology. However, in frozen samples, alterations of mitochondria were observed in all groups. Despite this, the integrity of the remained organelles was preserved in follicles cryopreserved with EG, while that in others groups (DMSO and association) an excess of vacuolizaton in cytoplasm of oocytes and swelling of nuclear membrane was observed indicating degeneration. Conclusion: The Ehilene Glycol seems to be the cryoprotector more adequated for cryopreservation of sheep ovarian tissue. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-07-01 2014-05-27T11:23:56Z 2014-05-27T11:23:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.researchgate.net/profile/Bruno_Carvalho7/publication/235990150_Use_of_ovarian_reserve_markers_and_variables_of_response_to_gonadotropic_stimulus_as_predictors_of_embryo_implantation_in_ICSI_cycles/file/5046351545001f25bb.pdf Jornal Brasileiro de Reproducao Assistida, v. 13, n. 3, p. 9-13, 2009. 1517-5693 1518-0557 http://hdl.handle.net/11449/71083 2-s2.0-77649137095 5017595072248917 8456490300814833 0000-0002-2420-2550 |
url |
http://www.researchgate.net/profile/Bruno_Carvalho7/publication/235990150_Use_of_ovarian_reserve_markers_and_variables_of_response_to_gonadotropic_stimulus_as_predictors_of_embryo_implantation_in_ICSI_cycles/file/5046351545001f25bb.pdf http://hdl.handle.net/11449/71083 |
identifier_str_mv |
Jornal Brasileiro de Reproducao Assistida, v. 13, n. 3, p. 9-13, 2009. 1517-5693 1518-0557 2-s2.0-77649137095 5017595072248917 8456490300814833 0000-0002-2420-2550 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Jornal Brasileiro de Reproducao Assistida 0,282 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9-13 |
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Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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repositoriounesp@unesp.br |
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1810021328900587520 |