Evaluation of serum- and animal protein-free media for the production of infectious bronchitis virus (M41) strain in a continuous cell line

Detalhes bibliográficos
Autor(a) principal: Cardoso, Tereza [UNESP]
Data de Publicação: 2005
Outros Autores: Teixeira, Maria C. B. [UNESP], Fachin, Natali [UNESP], Camargo, Tatiani L., Gomes, Deriane E., Corte Júnior, Anivaldo O., Anselmo, Beatriz [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.altex.ch/All-issues/Issue.50.html?iid=76&aid=4
http://hdl.handle.net/11449/68461
Resumo: Infectious bronchitis virus (IBV) is produced in vitro using specific pathogen free chicken embryos, primary chicken kidney cells (CKC) and/or tracheal organ culture (TOC). Regulatory authorities in Europe (EMEA) and in the United States (FDA) have encouraged biological manufactures to reduce or eliminate the use of live animals and/or products of animal origin in biological manufacturing processes. In this paper, a stable chicken embryo-related (CER) cell line was adapted and maintained in serum free (SF) or animal protein free (APF) medium after a direct switch of the medium. The most suitable media were Ex Cell 520 and Ex Cell 302. CER monolayers adapted to SF or APF were infected with IBV (M41 strain) in agitated suspended culture and the IBV titre obtained 48 h post infection was 2.8 × 104 PFU/ml in both cases. Thus, propagation of CER cells and culture of IBV can be performed without the use of animal serum or animal protein.
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spelling Evaluation of serum- and animal protein-free media for the production of infectious bronchitis virus (M41) strain in a continuous cell lineAnimal protein free mediaIBVSerum free mediaVirus productionanimal cellAvian infectious bronchitis viruscell linechickencontinuous culturecontrolled studyculture mediumembryoin vitro studymonolayer culturenonhumanserumvirus strainAnimalsBlood ProteinsChick EmbryoCulture MediaCulture Media, Serum-FreeInfectious bronchitis virusUnited StatesUnited States Food and Drug AdministrationInfectious bronchitis virus (IBV) is produced in vitro using specific pathogen free chicken embryos, primary chicken kidney cells (CKC) and/or tracheal organ culture (TOC). Regulatory authorities in Europe (EMEA) and in the United States (FDA) have encouraged biological manufactures to reduce or eliminate the use of live animals and/or products of animal origin in biological manufacturing processes. In this paper, a stable chicken embryo-related (CER) cell line was adapted and maintained in serum free (SF) or animal protein free (APF) medium after a direct switch of the medium. The most suitable media were Ex Cell 520 and Ex Cell 302. CER monolayers adapted to SF or APF were infected with IBV (M41 strain) in agitated suspended culture and the IBV titre obtained 48 h post infection was 2.8 × 104 PFU/ml in both cases. Thus, propagation of CER cells and culture of IBV can be performed without the use of animal serum or animal protein.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP-Laboratório de Virologia Curso de Medicina Veterinária Universidade Estadual Paulista-Araçatuba, Rua Clóvis Pestana 793, 16.050-680 Araçatuba, SPCurso de Mestrado e m Ciência Animal UNESP-Campus de Araçatuba, Br-São PauloFellowship Fundação de Amparo a Pesquisa do Estado de São Paulo, Br-São PauloFellowship Conselho Nacional de Desenvolvimento Científico e Tecnológico CNPq, Br-São PauloUNESP-Curso de Medicina Veterinŕia Faculdade de Odontologia Universidade Estadual Paulista-Araçatuba, Rua Clóvis Pestana 793, 16.050-680 Araçatuba, SPUNESP-Laboratório de Virologia Curso de Medicina Veterinária Universidade Estadual Paulista-Araçatuba, Rua Clóvis Pestana 793, 16.050-680 Araçatuba, SPCurso de Mestrado e m Ciência Animal UNESP-Campus de Araçatuba, Br-São PauloUNESP-Curso de Medicina Veterinŕia Faculdade de Odontologia Universidade Estadual Paulista-Araçatuba, Rua Clóvis Pestana 793, 16.050-680 Araçatuba, SPUniversidade Estadual Paulista (Unesp)Cardoso, Tereza [UNESP]Teixeira, Maria C. B. [UNESP]Fachin, Natali [UNESP]Camargo, Tatiani L.Gomes, Deriane E.Corte Júnior, Anivaldo O.Anselmo, Beatriz [UNESP]2014-05-27T11:21:39Z2014-05-27T11:21:39Z2005-10-31info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article152-156application/pdfhttp://www.altex.ch/All-issues/Issue.50.html?iid=76&aid=4Altex, v. 22, n. 3, p. 152-156, 2005.0946-7785http://hdl.handle.net/11449/68461WOS:0002325585000052-s2.0-271444789712-s2.0-27144478971.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAltex1,001info:eu-repo/semantics/openAccess2023-12-25T06:19:42Zoai:repositorio.unesp.br:11449/68461Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-25T06:19:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of serum- and animal protein-free media for the production of infectious bronchitis virus (M41) strain in a continuous cell line
title Evaluation of serum- and animal protein-free media for the production of infectious bronchitis virus (M41) strain in a continuous cell line
spellingShingle Evaluation of serum- and animal protein-free media for the production of infectious bronchitis virus (M41) strain in a continuous cell line
Cardoso, Tereza [UNESP]
Animal protein free media
IBV
Serum free media
Virus production
animal cell
Avian infectious bronchitis virus
cell line
chicken
continuous culture
controlled study
culture medium
embryo
in vitro study
monolayer culture
nonhuman
serum
virus strain
Animals
Blood Proteins
Chick Embryo
Culture Media
Culture Media, Serum-Free
Infectious bronchitis virus
United States
United States Food and Drug Administration
title_short Evaluation of serum- and animal protein-free media for the production of infectious bronchitis virus (M41) strain in a continuous cell line
title_full Evaluation of serum- and animal protein-free media for the production of infectious bronchitis virus (M41) strain in a continuous cell line
title_fullStr Evaluation of serum- and animal protein-free media for the production of infectious bronchitis virus (M41) strain in a continuous cell line
title_full_unstemmed Evaluation of serum- and animal protein-free media for the production of infectious bronchitis virus (M41) strain in a continuous cell line
title_sort Evaluation of serum- and animal protein-free media for the production of infectious bronchitis virus (M41) strain in a continuous cell line
author Cardoso, Tereza [UNESP]
author_facet Cardoso, Tereza [UNESP]
Teixeira, Maria C. B. [UNESP]
Fachin, Natali [UNESP]
Camargo, Tatiani L.
Gomes, Deriane E.
Corte Júnior, Anivaldo O.
Anselmo, Beatriz [UNESP]
author_role author
author2 Teixeira, Maria C. B. [UNESP]
Fachin, Natali [UNESP]
Camargo, Tatiani L.
Gomes, Deriane E.
Corte Júnior, Anivaldo O.
Anselmo, Beatriz [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Cardoso, Tereza [UNESP]
Teixeira, Maria C. B. [UNESP]
Fachin, Natali [UNESP]
Camargo, Tatiani L.
Gomes, Deriane E.
Corte Júnior, Anivaldo O.
Anselmo, Beatriz [UNESP]
dc.subject.por.fl_str_mv Animal protein free media
IBV
Serum free media
Virus production
animal cell
Avian infectious bronchitis virus
cell line
chicken
continuous culture
controlled study
culture medium
embryo
in vitro study
monolayer culture
nonhuman
serum
virus strain
Animals
Blood Proteins
Chick Embryo
Culture Media
Culture Media, Serum-Free
Infectious bronchitis virus
United States
United States Food and Drug Administration
topic Animal protein free media
IBV
Serum free media
Virus production
animal cell
Avian infectious bronchitis virus
cell line
chicken
continuous culture
controlled study
culture medium
embryo
in vitro study
monolayer culture
nonhuman
serum
virus strain
Animals
Blood Proteins
Chick Embryo
Culture Media
Culture Media, Serum-Free
Infectious bronchitis virus
United States
United States Food and Drug Administration
description Infectious bronchitis virus (IBV) is produced in vitro using specific pathogen free chicken embryos, primary chicken kidney cells (CKC) and/or tracheal organ culture (TOC). Regulatory authorities in Europe (EMEA) and in the United States (FDA) have encouraged biological manufactures to reduce or eliminate the use of live animals and/or products of animal origin in biological manufacturing processes. In this paper, a stable chicken embryo-related (CER) cell line was adapted and maintained in serum free (SF) or animal protein free (APF) medium after a direct switch of the medium. The most suitable media were Ex Cell 520 and Ex Cell 302. CER monolayers adapted to SF or APF were infected with IBV (M41 strain) in agitated suspended culture and the IBV titre obtained 48 h post infection was 2.8 × 104 PFU/ml in both cases. Thus, propagation of CER cells and culture of IBV can be performed without the use of animal serum or animal protein.
publishDate 2005
dc.date.none.fl_str_mv 2005-10-31
2014-05-27T11:21:39Z
2014-05-27T11:21:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.altex.ch/All-issues/Issue.50.html?iid=76&aid=4
Altex, v. 22, n. 3, p. 152-156, 2005.
0946-7785
http://hdl.handle.net/11449/68461
WOS:000232558500005
2-s2.0-27144478971
2-s2.0-27144478971.pdf
url http://www.altex.ch/All-issues/Issue.50.html?iid=76&aid=4
http://hdl.handle.net/11449/68461
identifier_str_mv Altex, v. 22, n. 3, p. 152-156, 2005.
0946-7785
WOS:000232558500005
2-s2.0-27144478971
2-s2.0-27144478971.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Altex
1,001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 152-156
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799965400157388800

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