Prevalence, Antimicrobial Resistance, and Diversity of Salmonella along the Pig Production Chain in Southern Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/pathogens8040204 http://hdl.handle.net/11449/196478 |
Resumo: | Control of Salmonella spp. in food production chains is very important to ensure safe foods and minimize the risks of foodborne disease occurrence. This study aimed to identify the prevalence and main contamination sources of Salmonella spp. in a pig production chain in southern Brazil. Six lots of piglets produced at different farms were tracked until their slaughter, and samples were subjected to Salmonella spp. detection. The obtained isolates were serotyped, subjected to antimicrobial resistance testing, and pulsed field gel electrophoresis (PFGE). Salmonella spp. was detected in 160 (10.2%) samples, and not detected in pig carcasses after final washing or chilling. Among the 210 Salmonella spp. isolates, S. Typhimurium was the most prevalent (n = 101) and resistant to at least one antimicrobial. High resistance rates were detected against tetracycline (83.8%), chloramphenicol (54.3%), and trimethoprim-sulfamethoxazole (33.3%). The isolates that were non-susceptible to three or more classes of antimicrobials (n = 60) were considered multidrug-resistant (MDR), and isolates resistant to up to six of the tested antimicrobials were found. PFGE allowed the identification of genetic diversity and demonstrated that farm environment and feed supply may be sources for the dissemination of Salmonella spp. along the production chain. The results revealed the sources of Salmonella contamination in the pig production chain and highlighted the risks of antimicrobial resistance spread. |
id |
UNSP_7287748565a5ddd946f6cfb6aec31a79 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/196478 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Prevalence, Antimicrobial Resistance, and Diversity of Salmonella along the Pig Production Chain in Southern BrazilSalmonellapig production chainPFGEcontamination routesresistanceControl of Salmonella spp. in food production chains is very important to ensure safe foods and minimize the risks of foodborne disease occurrence. This study aimed to identify the prevalence and main contamination sources of Salmonella spp. in a pig production chain in southern Brazil. Six lots of piglets produced at different farms were tracked until their slaughter, and samples were subjected to Salmonella spp. detection. The obtained isolates were serotyped, subjected to antimicrobial resistance testing, and pulsed field gel electrophoresis (PFGE). Salmonella spp. was detected in 160 (10.2%) samples, and not detected in pig carcasses after final washing or chilling. Among the 210 Salmonella spp. isolates, S. Typhimurium was the most prevalent (n = 101) and resistant to at least one antimicrobial. High resistance rates were detected against tetracycline (83.8%), chloramphenicol (54.3%), and trimethoprim-sulfamethoxazole (33.3%). The isolates that were non-susceptible to three or more classes of antimicrobials (n = 60) were considered multidrug-resistant (MDR), and isolates resistant to up to six of the tested antimicrobials were found. PFGE allowed the identification of genetic diversity and demonstrated that farm environment and feed supply may be sources for the dissemination of Salmonella spp. along the production chain. The results revealed the sources of Salmonella contamination in the pig production chain and highlighted the risks of antimicrobial resistance spread.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Parana, Dept Ciencia Vet, Setor Palotina, BR-85950000 Palotina, BrazilUniv Fed Vicosa, Dept Vet, BR-36570900 Vicosa, MG, BrazilUniv Estadual Paulista, Dept Higiene Vet & Saude Publ, BR-18618000 Botucatu, SP, BrazilUniv Sao Paulo, Fac Ciencias Farmaceut, BR-05508900 Sao Paulo, SP, BrazilUniv Estadual Paulista, Dept Higiene Vet & Saude Publ, BR-18618000 Botucatu, SP, BrazilMdpiUniv Fed ParanaUniversidade Federal de Viçosa (UFV)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Santos Bersot, Luciano dosQuintana Cavicchioli, ValeriaViana, CibeliKonrad Burin, Raquel CristinaCamargo, Anderson CarlosNogueira Pinto, Jose Paes de Almeida [UNESP]Nero, Luis AugustoDestro, Maria Teresa2020-12-10T19:46:17Z2020-12-10T19:46:17Z2019-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10http://dx.doi.org/10.3390/pathogens8040204Pathogens. Basel: Mdpi, v. 8, n. 4, 10 p., 2019.http://hdl.handle.net/11449/19647810.3390/pathogens8040204WOS:000506652300047Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPathogensinfo:eu-repo/semantics/openAccess2021-10-23T07:07:12Zoai:repositorio.unesp.br:11449/196478Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:31:00.613615Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Prevalence, Antimicrobial Resistance, and Diversity of Salmonella along the Pig Production Chain in Southern Brazil |
title |
Prevalence, Antimicrobial Resistance, and Diversity of Salmonella along the Pig Production Chain in Southern Brazil |
spellingShingle |
Prevalence, Antimicrobial Resistance, and Diversity of Salmonella along the Pig Production Chain in Southern Brazil Santos Bersot, Luciano dos Salmonella pig production chain PFGE contamination routes resistance |
title_short |
Prevalence, Antimicrobial Resistance, and Diversity of Salmonella along the Pig Production Chain in Southern Brazil |
title_full |
Prevalence, Antimicrobial Resistance, and Diversity of Salmonella along the Pig Production Chain in Southern Brazil |
title_fullStr |
Prevalence, Antimicrobial Resistance, and Diversity of Salmonella along the Pig Production Chain in Southern Brazil |
title_full_unstemmed |
Prevalence, Antimicrobial Resistance, and Diversity of Salmonella along the Pig Production Chain in Southern Brazil |
title_sort |
Prevalence, Antimicrobial Resistance, and Diversity of Salmonella along the Pig Production Chain in Southern Brazil |
author |
Santos Bersot, Luciano dos |
author_facet |
Santos Bersot, Luciano dos Quintana Cavicchioli, Valeria Viana, Cibeli Konrad Burin, Raquel Cristina Camargo, Anderson Carlos Nogueira Pinto, Jose Paes de Almeida [UNESP] Nero, Luis Augusto Destro, Maria Teresa |
author_role |
author |
author2 |
Quintana Cavicchioli, Valeria Viana, Cibeli Konrad Burin, Raquel Cristina Camargo, Anderson Carlos Nogueira Pinto, Jose Paes de Almeida [UNESP] Nero, Luis Augusto Destro, Maria Teresa |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Fed Parana Universidade Federal de Viçosa (UFV) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Santos Bersot, Luciano dos Quintana Cavicchioli, Valeria Viana, Cibeli Konrad Burin, Raquel Cristina Camargo, Anderson Carlos Nogueira Pinto, Jose Paes de Almeida [UNESP] Nero, Luis Augusto Destro, Maria Teresa |
dc.subject.por.fl_str_mv |
Salmonella pig production chain PFGE contamination routes resistance |
topic |
Salmonella pig production chain PFGE contamination routes resistance |
description |
Control of Salmonella spp. in food production chains is very important to ensure safe foods and minimize the risks of foodborne disease occurrence. This study aimed to identify the prevalence and main contamination sources of Salmonella spp. in a pig production chain in southern Brazil. Six lots of piglets produced at different farms were tracked until their slaughter, and samples were subjected to Salmonella spp. detection. The obtained isolates were serotyped, subjected to antimicrobial resistance testing, and pulsed field gel electrophoresis (PFGE). Salmonella spp. was detected in 160 (10.2%) samples, and not detected in pig carcasses after final washing or chilling. Among the 210 Salmonella spp. isolates, S. Typhimurium was the most prevalent (n = 101) and resistant to at least one antimicrobial. High resistance rates were detected against tetracycline (83.8%), chloramphenicol (54.3%), and trimethoprim-sulfamethoxazole (33.3%). The isolates that were non-susceptible to three or more classes of antimicrobials (n = 60) were considered multidrug-resistant (MDR), and isolates resistant to up to six of the tested antimicrobials were found. PFGE allowed the identification of genetic diversity and demonstrated that farm environment and feed supply may be sources for the dissemination of Salmonella spp. along the production chain. The results revealed the sources of Salmonella contamination in the pig production chain and highlighted the risks of antimicrobial resistance spread. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-01 2020-12-10T19:46:17Z 2020-12-10T19:46:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/pathogens8040204 Pathogens. Basel: Mdpi, v. 8, n. 4, 10 p., 2019. http://hdl.handle.net/11449/196478 10.3390/pathogens8040204 WOS:000506652300047 |
url |
http://dx.doi.org/10.3390/pathogens8040204 http://hdl.handle.net/11449/196478 |
identifier_str_mv |
Pathogens. Basel: Mdpi, v. 8, n. 4, 10 p., 2019. 10.3390/pathogens8040204 WOS:000506652300047 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pathogens |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 |
dc.publisher.none.fl_str_mv |
Mdpi |
publisher.none.fl_str_mv |
Mdpi |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129213763420160 |