Evaluation of Candida rugosa Lipase Immobilized on Magnetic Nanoparticles in Enzymatic/Chemical Hydroesterification for Biodiesel Production

Detalhes bibliográficos
Autor(a) principal: Domingues, Otávio [UNESP]
Data de Publicação: 2022
Outros Autores: Remonatto, Daniela [UNESP], dos Santos, Letícia Karen [UNESP], Galán, Julián Paul Martínez, Flumignan, Danilo Luiz [UNESP], de Paula, Ariela Veloso [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s12010-022-04046-9
http://hdl.handle.net/11449/241276
Resumo: This study aimed to (i) prepare functionalized maghemite nanoparticles for immobilization of Candida rugosa lipase (CRL) by covalent binding, (ii) evaluate the application of the immobilized derivative in the hydrolysis of waste cooking oil (WCO) to fatty acids, and (iii) assess the potential of the hydrolyzed material for biodiesel production by hydroesterification. Maghemite (γFe2O3) obtained by precipitation of Fe3Cl2 with NH4OH served as an efficient support for covalent immobilization of CRL. Fourier-transform infrared spectroscopy and hydrolytic activity analysis indicated that CRL was covalently immobilized on the surface of the maghemite support. The derivative showed an activity of 166.62 ± 8 U g−1 in WCO hydrolysis at 40 °C and pH 6. Scanning electron microscopy revealed that, after lipase immobilization, nanoparticles became more dispersed, which is advantageous for biocatalysis reactions, as it increases the contact area with the substrate. WCO hydrolysis afforded 96 ± 0.2 wt% free fatty acids. In the second step, free fatty acids were subjected to chemical esterification with sulfuric acid, affording 94.4 ± 0.02 wt% fatty acid methyl esters (biodiesel). The findings of this study contribute to the field of biotechnology and may promote the development of enzymatic technologies for the synthesis of products of economic and social interest.
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spelling Evaluation of Candida rugosa Lipase Immobilized on Magnetic Nanoparticles in Enzymatic/Chemical Hydroesterification for Biodiesel ProductionCandida rugosa lipaseChemical esterificationCovalent immobilizationEnzymeHydroesterificationMagnetic nanoparticlesThis study aimed to (i) prepare functionalized maghemite nanoparticles for immobilization of Candida rugosa lipase (CRL) by covalent binding, (ii) evaluate the application of the immobilized derivative in the hydrolysis of waste cooking oil (WCO) to fatty acids, and (iii) assess the potential of the hydrolyzed material for biodiesel production by hydroesterification. Maghemite (γFe2O3) obtained by precipitation of Fe3Cl2 with NH4OH served as an efficient support for covalent immobilization of CRL. Fourier-transform infrared spectroscopy and hydrolytic activity analysis indicated that CRL was covalently immobilized on the surface of the maghemite support. The derivative showed an activity of 166.62 ± 8 U g−1 in WCO hydrolysis at 40 °C and pH 6. Scanning electron microscopy revealed that, after lipase immobilization, nanoparticles became more dispersed, which is advantageous for biocatalysis reactions, as it increases the contact area with the substrate. WCO hydrolysis afforded 96 ± 0.2 wt% free fatty acids. In the second step, free fatty acids were subjected to chemical esterification with sulfuric acid, affording 94.4 ± 0.02 wt% fatty acid methyl esters (biodiesel). The findings of this study contribute to the field of biotechnology and may promote the development of enzymatic technologies for the synthesis of products of economic and social interest.Department of Engineering of Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP), SPDepartment of Organic Chemistry Center for Monitoring and Research of the Quality of Fuels Biofuels Crude Oil and Derivatives Institute of Chemistry São Paulo State University (UNESP), SPSchool of Nutrition and Dietetic University of Antioquia (UdeA)Mato Grosso Federal Institute of Education Science and Technology (IFSC), MTDepartment of Engineering of Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP), SPDepartment of Organic Chemistry Center for Monitoring and Research of the Quality of Fuels Biofuels Crude Oil and Derivatives Institute of Chemistry São Paulo State University (UNESP), SPUniversidade Estadual Paulista (UNESP)University of Antioquia (UdeA)Science and Technology (IFSC)Domingues, Otávio [UNESP]Remonatto, Daniela [UNESP]dos Santos, Letícia Karen [UNESP]Galán, Julián Paul MartínezFlumignan, Danilo Luiz [UNESP]de Paula, Ariela Veloso [UNESP]2023-03-01T20:54:45Z2023-03-01T20:54:45Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s12010-022-04046-9Applied Biochemistry and Biotechnology.1559-02910273-2289http://hdl.handle.net/11449/24127610.1007/s12010-022-04046-92-s2.0-85133483598Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplied Biochemistry and Biotechnologyinfo:eu-repo/semantics/openAccess2023-03-01T20:54:46Zoai:repositorio.unesp.br:11449/241276Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:50:50.877116Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of Candida rugosa Lipase Immobilized on Magnetic Nanoparticles in Enzymatic/Chemical Hydroesterification for Biodiesel Production
title Evaluation of Candida rugosa Lipase Immobilized on Magnetic Nanoparticles in Enzymatic/Chemical Hydroesterification for Biodiesel Production
spellingShingle Evaluation of Candida rugosa Lipase Immobilized on Magnetic Nanoparticles in Enzymatic/Chemical Hydroesterification for Biodiesel Production
Domingues, Otávio [UNESP]
Candida rugosa lipase
Chemical esterification
Covalent immobilization
Enzyme
Hydroesterification
Magnetic nanoparticles
title_short Evaluation of Candida rugosa Lipase Immobilized on Magnetic Nanoparticles in Enzymatic/Chemical Hydroesterification for Biodiesel Production
title_full Evaluation of Candida rugosa Lipase Immobilized on Magnetic Nanoparticles in Enzymatic/Chemical Hydroesterification for Biodiesel Production
title_fullStr Evaluation of Candida rugosa Lipase Immobilized on Magnetic Nanoparticles in Enzymatic/Chemical Hydroesterification for Biodiesel Production
title_full_unstemmed Evaluation of Candida rugosa Lipase Immobilized on Magnetic Nanoparticles in Enzymatic/Chemical Hydroesterification for Biodiesel Production
title_sort Evaluation of Candida rugosa Lipase Immobilized on Magnetic Nanoparticles in Enzymatic/Chemical Hydroesterification for Biodiesel Production
author Domingues, Otávio [UNESP]
author_facet Domingues, Otávio [UNESP]
Remonatto, Daniela [UNESP]
dos Santos, Letícia Karen [UNESP]
Galán, Julián Paul Martínez
Flumignan, Danilo Luiz [UNESP]
de Paula, Ariela Veloso [UNESP]
author_role author
author2 Remonatto, Daniela [UNESP]
dos Santos, Letícia Karen [UNESP]
Galán, Julián Paul Martínez
Flumignan, Danilo Luiz [UNESP]
de Paula, Ariela Veloso [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
University of Antioquia (UdeA)
Science and Technology (IFSC)
dc.contributor.author.fl_str_mv Domingues, Otávio [UNESP]
Remonatto, Daniela [UNESP]
dos Santos, Letícia Karen [UNESP]
Galán, Julián Paul Martínez
Flumignan, Danilo Luiz [UNESP]
de Paula, Ariela Veloso [UNESP]
dc.subject.por.fl_str_mv Candida rugosa lipase
Chemical esterification
Covalent immobilization
Enzyme
Hydroesterification
Magnetic nanoparticles
topic Candida rugosa lipase
Chemical esterification
Covalent immobilization
Enzyme
Hydroesterification
Magnetic nanoparticles
description This study aimed to (i) prepare functionalized maghemite nanoparticles for immobilization of Candida rugosa lipase (CRL) by covalent binding, (ii) evaluate the application of the immobilized derivative in the hydrolysis of waste cooking oil (WCO) to fatty acids, and (iii) assess the potential of the hydrolyzed material for biodiesel production by hydroesterification. Maghemite (γFe2O3) obtained by precipitation of Fe3Cl2 with NH4OH served as an efficient support for covalent immobilization of CRL. Fourier-transform infrared spectroscopy and hydrolytic activity analysis indicated that CRL was covalently immobilized on the surface of the maghemite support. The derivative showed an activity of 166.62 ± 8 U g−1 in WCO hydrolysis at 40 °C and pH 6. Scanning electron microscopy revealed that, after lipase immobilization, nanoparticles became more dispersed, which is advantageous for biocatalysis reactions, as it increases the contact area with the substrate. WCO hydrolysis afforded 96 ± 0.2 wt% free fatty acids. In the second step, free fatty acids were subjected to chemical esterification with sulfuric acid, affording 94.4 ± 0.02 wt% fatty acid methyl esters (biodiesel). The findings of this study contribute to the field of biotechnology and may promote the development of enzymatic technologies for the synthesis of products of economic and social interest.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
2023-03-01T20:54:45Z
2023-03-01T20:54:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s12010-022-04046-9
Applied Biochemistry and Biotechnology.
1559-0291
0273-2289
http://hdl.handle.net/11449/241276
10.1007/s12010-022-04046-9
2-s2.0-85133483598
url http://dx.doi.org/10.1007/s12010-022-04046-9
http://hdl.handle.net/11449/241276
identifier_str_mv Applied Biochemistry and Biotechnology.
1559-0291
0273-2289
10.1007/s12010-022-04046-9
2-s2.0-85133483598
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Applied Biochemistry and Biotechnology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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