Avian coronavirus isolated from a pigeon sample induced clinical disease, tracheal ciliostasis, and a high humoral response in day-old chicks
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/03079457.2018.1442557 http://hdl.handle.net/11449/164164 |
Resumo: | The detection of avian coronaviruses (AvCoV) in wild birds and the emergence of new AvCoV have increased in the past few years. In the present study, the pathogenicity of three AvCoV isolates was investigated in day-old chicks. One AvCoV isolated from a pigeon, which clustered with the Massachusetts vaccine serotype, and two AvCoV isolated from chickens, which grouped with a Brazilian genotype lineage, were used. Clinical signs, gross lesions, histopathological changes, ciliary activity, viral RNA detection, and serology were evaluated during 42 days post infection. All AvCoV isolates induced clinical signs, gross lesions in the trachea, moderate histopathological changes in the respiratory tract, and mild changes in other tissues. AvCoV isolated from the pigeon sample caused complete tracheal ciliostasis over a longer time span. Specific viral RNA was detected in all tissues, but the highest RNA loads were detected in the digestive tract (cloacal swabs and ileum). The highest antibody levels were also detected in the group infected with an isolate from the pigeon. These results confirm the pathogenicity of Brazilian variants, which can cause disease and induce gross lesions and histopathological changes in chickens. Our results suggest that non-Galliformes birds can also play a role in the ecology of AvCoV. |
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Avian coronavirus isolated from a pigeon sample induced clinical disease, tracheal ciliostasis, and a high humoral response in day-old chicksInfectious bronchitis viruspigeonchickenpathogenicityciliostasisThe detection of avian coronaviruses (AvCoV) in wild birds and the emergence of new AvCoV have increased in the past few years. In the present study, the pathogenicity of three AvCoV isolates was investigated in day-old chicks. One AvCoV isolated from a pigeon, which clustered with the Massachusetts vaccine serotype, and two AvCoV isolated from chickens, which grouped with a Brazilian genotype lineage, were used. Clinical signs, gross lesions, histopathological changes, ciliary activity, viral RNA detection, and serology were evaluated during 42 days post infection. All AvCoV isolates induced clinical signs, gross lesions in the trachea, moderate histopathological changes in the respiratory tract, and mild changes in other tissues. AvCoV isolated from the pigeon sample caused complete tracheal ciliostasis over a longer time span. Specific viral RNA was detected in all tissues, but the highest RNA loads were detected in the digestive tract (cloacal swabs and ileum). The highest antibody levels were also detected in the group infected with an isolate from the pigeon. These results confirm the pathogenicity of Brazilian variants, which can cause disease and induce gross lesions and histopathological changes in chickens. Our results suggest that non-Galliformes birds can also play a role in the ecology of AvCoV.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Campinas, UNICAMP, Inst Biol, Lab Anim Virol, Campinas, SP, BrazilUniv Sao Paulo, FZEA, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga, SP, BrazilUniv Sao Paulo, FMVZ, Fac Vet Med & Anim Sci, Postgrad Program Expt Epidemiol Zoonoses, Sao Paulo, SP, BrazilUniv Fed Juiz de Fora, ICB, Juiz De Fora, MG, BrazilUniv Estadual Paulista, Coll Vet Med, Lab Anim Virol & Cell Culture, DAPSA Dept, Aracatuba, SP, BrazilUniv Estadual Paulista, Coll Vet Med, Lab Anim Virol & Cell Culture, DAPSA Dept, Aracatuba, SP, BrazilFAPESP: 2014/50696-4FAPESP: 2011/50919-5FAPESP: 2015/11510-5Taylor & Francis LtdUniversidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Univ Fed Juiz de ForaMartini, Matheus C.Caserta, Leonardo C.Santos, Marcia M. A. B. dosBarnabe, Ana C. S.Duraes-Carvalho, RicardoPadilla, Marina A.Simao, Raphael M.Rizotto, Lais S.Simas, Paulo V. M.Bastos, Juliana C. S.Cardoso, Tereza C. [UNESP]Felippe, Paulo A. N.Ferreira, Helena L.Arns, Clarice W.2018-11-26T17:51:31Z2018-11-26T17:51:31Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article286-293application/pdfhttp://dx.doi.org/10.1080/03079457.2018.1442557Avian Pathology. Abingdon: Taylor & Francis Ltd, v. 47, n. 3, p. 286-293, 2018.0307-9457http://hdl.handle.net/11449/16416410.1080/03079457.2018.1442557WOS:000431523500009WOS000431523500009.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAvian Pathology0,871info:eu-repo/semantics/openAccess2024-09-04T19:15:53Zoai:repositorio.unesp.br:11449/164164Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Avian coronavirus isolated from a pigeon sample induced clinical disease, tracheal ciliostasis, and a high humoral response in day-old chicks |
title |
Avian coronavirus isolated from a pigeon sample induced clinical disease, tracheal ciliostasis, and a high humoral response in day-old chicks |
spellingShingle |
Avian coronavirus isolated from a pigeon sample induced clinical disease, tracheal ciliostasis, and a high humoral response in day-old chicks Martini, Matheus C. Infectious bronchitis virus pigeon chicken pathogenicity ciliostasis |
title_short |
Avian coronavirus isolated from a pigeon sample induced clinical disease, tracheal ciliostasis, and a high humoral response in day-old chicks |
title_full |
Avian coronavirus isolated from a pigeon sample induced clinical disease, tracheal ciliostasis, and a high humoral response in day-old chicks |
title_fullStr |
Avian coronavirus isolated from a pigeon sample induced clinical disease, tracheal ciliostasis, and a high humoral response in day-old chicks |
title_full_unstemmed |
Avian coronavirus isolated from a pigeon sample induced clinical disease, tracheal ciliostasis, and a high humoral response in day-old chicks |
title_sort |
Avian coronavirus isolated from a pigeon sample induced clinical disease, tracheal ciliostasis, and a high humoral response in day-old chicks |
author |
Martini, Matheus C. |
author_facet |
Martini, Matheus C. Caserta, Leonardo C. Santos, Marcia M. A. B. dos Barnabe, Ana C. S. Duraes-Carvalho, Ricardo Padilla, Marina A. Simao, Raphael M. Rizotto, Lais S. Simas, Paulo V. M. Bastos, Juliana C. S. Cardoso, Tereza C. [UNESP] Felippe, Paulo A. N. Ferreira, Helena L. Arns, Clarice W. |
author_role |
author |
author2 |
Caserta, Leonardo C. Santos, Marcia M. A. B. dos Barnabe, Ana C. S. Duraes-Carvalho, Ricardo Padilla, Marina A. Simao, Raphael M. Rizotto, Lais S. Simas, Paulo V. M. Bastos, Juliana C. S. Cardoso, Tereza C. [UNESP] Felippe, Paulo A. N. Ferreira, Helena L. Arns, Clarice W. |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Univ Fed Juiz de Fora |
dc.contributor.author.fl_str_mv |
Martini, Matheus C. Caserta, Leonardo C. Santos, Marcia M. A. B. dos Barnabe, Ana C. S. Duraes-Carvalho, Ricardo Padilla, Marina A. Simao, Raphael M. Rizotto, Lais S. Simas, Paulo V. M. Bastos, Juliana C. S. Cardoso, Tereza C. [UNESP] Felippe, Paulo A. N. Ferreira, Helena L. Arns, Clarice W. |
dc.subject.por.fl_str_mv |
Infectious bronchitis virus pigeon chicken pathogenicity ciliostasis |
topic |
Infectious bronchitis virus pigeon chicken pathogenicity ciliostasis |
description |
The detection of avian coronaviruses (AvCoV) in wild birds and the emergence of new AvCoV have increased in the past few years. In the present study, the pathogenicity of three AvCoV isolates was investigated in day-old chicks. One AvCoV isolated from a pigeon, which clustered with the Massachusetts vaccine serotype, and two AvCoV isolated from chickens, which grouped with a Brazilian genotype lineage, were used. Clinical signs, gross lesions, histopathological changes, ciliary activity, viral RNA detection, and serology were evaluated during 42 days post infection. All AvCoV isolates induced clinical signs, gross lesions in the trachea, moderate histopathological changes in the respiratory tract, and mild changes in other tissues. AvCoV isolated from the pigeon sample caused complete tracheal ciliostasis over a longer time span. Specific viral RNA was detected in all tissues, but the highest RNA loads were detected in the digestive tract (cloacal swabs and ileum). The highest antibody levels were also detected in the group infected with an isolate from the pigeon. These results confirm the pathogenicity of Brazilian variants, which can cause disease and induce gross lesions and histopathological changes in chickens. Our results suggest that non-Galliformes birds can also play a role in the ecology of AvCoV. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:51:31Z 2018-11-26T17:51:31Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/03079457.2018.1442557 Avian Pathology. Abingdon: Taylor & Francis Ltd, v. 47, n. 3, p. 286-293, 2018. 0307-9457 http://hdl.handle.net/11449/164164 10.1080/03079457.2018.1442557 WOS:000431523500009 WOS000431523500009.pdf |
url |
http://dx.doi.org/10.1080/03079457.2018.1442557 http://hdl.handle.net/11449/164164 |
identifier_str_mv |
Avian Pathology. Abingdon: Taylor & Francis Ltd, v. 47, n. 3, p. 286-293, 2018. 0307-9457 10.1080/03079457.2018.1442557 WOS:000431523500009 WOS000431523500009.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Avian Pathology 0,871 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
286-293 application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis Ltd |
publisher.none.fl_str_mv |
Taylor & Francis Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021409126088704 |