A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis

Detalhes bibliográficos
Autor(a) principal: Melo, Stella Maria Barrouin
Data de Publicação: 2006
Outros Autores: Larangeira, Daniela Farias, Santos, Silvana Ornelas, Chagas Júnior, Adenizar Delgado das, Paixão, Mariza, Aguiar, Paulo Henrique Palis, Santos, Washington Luis Conrado dos, Carvalho, Lain Carlos Pontes de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/6499
Resumo: RESTRITO
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spelling Melo, Stella Maria BarrouinLarangeira, Daniela FariasSantos, Silvana OrnelasChagas Júnior, Adenizar Delgado dasPaixão, MarizaAguiar, Paulo Henrique PalisSantos, Washington Luis Conrado dosCarvalho, Lain Carlos Pontes deMelo, Stella Maria BarrouinLarangeira, Daniela FariasSantos, Silvana OrnelasChagas Júnior, Adenizar Delgado dasPaixão, MarizaAguiar, Paulo Henrique PalisSantos, Washington Luis Conrado dosCarvalho, Lain Carlos Pontes de2012-07-26T19:48:41Z2012-07-26T19:48:41Z20060165-2427http://www.repositorio.ufba.br/ri/handle/ri/6499v.111, n.3-4RESTRITOA method for the evaluation of splenic cellularity using samples collected by fine-needle aspirative biopsy was standardized in this work. The procedure includes erythrocyte lysing, preparation of cytospin films and staining by histochemical and immunocytochemical techniques. The cellular profiles of spleen preparations were compared with those observed in peripheral blood samples subjected to the same procedure. Two groups were compared, one consisting of 14 healthy uninfected and the other of 15 polysymptomatic Leishmania chagasi/infantum-infected dogs, from an endemic area for visceral leishmaniosis. Cell populations were identified by conventional hematoxilin–eosin and Wright’ stainings, and by immunocytochemistry using monoclonal antibodies against canine CD45RA and CD45RB, phagocytes and a pan-leukocyte antigen. Larger neutrophil (P < 0.0001) and monocyte/macrophage (P = 0.0036) relative counts and lower lymphocyte relative counts (P < 0.0001) were found in the spleen, and not in the blood, of the animals with leishmaniosis than in those of the healthy animals. The proportions of CD45RB+ cells were higher, and of CD45RA+ cells were lower, both in the spleen and in the blood of animals with leishmaniosis than in those of healthy dogs (P < 0.05). Additionally, hematoxilin–eosin-stained cytospins of spleen aspirates from Leishmania-infected animals permitted the easy visualization of amastigote forms inside phagocytes, under light microscopy.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2012-07-26T19:48:41Z No. of bitstreams: 1 GG.pdf: 337692 bytes, checksum: 085d3627daac4f4f547fcda56dfbede8 (MD5)Made available in DSpace on 2012-07-26T19:48:41Z (GMT). No. of bitstreams: 1 GG.pdf: 337692 bytes, checksum: 085d3627daac4f4f547fcda56dfbede8 (MD5) Previous issue date: 2006SalvadorVeterinary Immunology and Immunopathologyhttp://dx.doi.org/10.1016/j.vetimm.2006.01.021reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBASpleenCanineFine-needle aspirative biopsyLeukocyte populationsCanine visceral leishmaniosisA standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosisVeterinary Immunology and Immunopathologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/6499/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52ORIGINALGG.pdfGG.pdfapplication/pdf337692https://repositorio.ufba.br/bitstream/ri/6499/1/GG.pdf085d3627daac4f4f547fcda56dfbede8MD51TEXTGG.pdf.txtGG.pdf.txtExtracted texttext/plain43460https://repositorio.ufba.br/bitstream/ri/6499/3/GG.pdf.txtec6659ed9b18ce1cc1e3135cccd49839MD53ri/64992022-07-05 14:03:10.535oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:10Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis
dc.title.alternative.pt_BR.fl_str_mv Veterinary Immunology and Immunopathology
title A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis
spellingShingle A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis
Melo, Stella Maria Barrouin
Spleen
Canine
Fine-needle aspirative biopsy
Leukocyte populations
Canine visceral leishmaniosis
title_short A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis
title_full A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis
title_fullStr A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis
title_full_unstemmed A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis
title_sort A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis
author Melo, Stella Maria Barrouin
author_facet Melo, Stella Maria Barrouin
Larangeira, Daniela Farias
Santos, Silvana Ornelas
Chagas Júnior, Adenizar Delgado das
Paixão, Mariza
Aguiar, Paulo Henrique Palis
Santos, Washington Luis Conrado dos
Carvalho, Lain Carlos Pontes de
author_role author
author2 Larangeira, Daniela Farias
Santos, Silvana Ornelas
Chagas Júnior, Adenizar Delgado das
Paixão, Mariza
Aguiar, Paulo Henrique Palis
Santos, Washington Luis Conrado dos
Carvalho, Lain Carlos Pontes de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Melo, Stella Maria Barrouin
Larangeira, Daniela Farias
Santos, Silvana Ornelas
Chagas Júnior, Adenizar Delgado das
Paixão, Mariza
Aguiar, Paulo Henrique Palis
Santos, Washington Luis Conrado dos
Carvalho, Lain Carlos Pontes de
Melo, Stella Maria Barrouin
Larangeira, Daniela Farias
Santos, Silvana Ornelas
Chagas Júnior, Adenizar Delgado das
Paixão, Mariza
Aguiar, Paulo Henrique Palis
Santos, Washington Luis Conrado dos
Carvalho, Lain Carlos Pontes de
dc.subject.por.fl_str_mv Spleen
Canine
Fine-needle aspirative biopsy
Leukocyte populations
Canine visceral leishmaniosis
topic Spleen
Canine
Fine-needle aspirative biopsy
Leukocyte populations
Canine visceral leishmaniosis
description RESTRITO
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2012-07-26T19:48:41Z
dc.date.available.fl_str_mv 2012-07-26T19:48:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/6499
dc.identifier.issn.none.fl_str_mv 0165-2427
dc.identifier.number.pt_BR.fl_str_mv v.111, n.3-4
identifier_str_mv 0165-2427
v.111, n.3-4
url http://www.repositorio.ufba.br/ri/handle/ri/6499
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Veterinary Immunology and Immunopathology
publisher.none.fl_str_mv Veterinary Immunology and Immunopathology
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.vetimm.2006.01.021
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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