Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/228346 |
Resumo: | The aim of this study was to characterize chemically a sample of brown propolis and determine the time of action needed for eliminating microorganisms causing bovine mastitis. It was prepared hydroalcoholic extract of brown propolis (25 mg/mL) and this was evaluated for content of total phenolic compounds, total flavonoids, qualitative and quantitative analysis of phenolic compounds by liquid chromatography coupled to mass spectrometry and antibacterial activity against Escherichia coli, Staphylococcus spp, Streptococcus spp. It was found 34.39% and 13.46% of flavonoids and phenolic compounds, respectively, among which stand out ferulic acid (0.09 mg/g), caffeic acid (0.17 mg/g) and p-coumaric acid (3.39 mg/g). In the first 15 minutes incubation, all counts differed from the initial counts (p<0.01). With 15 minutes of exposure to the extract, Escherichia coli was inhibited by 38.72% and differed from the other bacterial genus (p<0.01, n=6). Staphylococcus spp. has had inhibition of 46.03% and Streptotococcus spp. of 50.8%. After 2 hours incubation, the genus Streptococcus was totally eliminated, Staphylococcus spp. after 3 hours and E. coli after 4 hours. Clearly, E. coli was more resistant bacteria than S. aureus. The other species of Staphylococcus and Streptococcus did not show large differences between the counts. We concluded that the composition of the brown propolis sample from Southern Brazil showed large levels of phenolic acids with proven antibacterial action, what explains the bactericidal activity found. |
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Chemical characterization and antibacterial action of brown propolis extract from Southern BrazilCaracterização química e ação antibacteriana de extrato de própolis marrom da região sul do BrasilAction timeAntimicrobialNatural productsThe aim of this study was to characterize chemically a sample of brown propolis and determine the time of action needed for eliminating microorganisms causing bovine mastitis. It was prepared hydroalcoholic extract of brown propolis (25 mg/mL) and this was evaluated for content of total phenolic compounds, total flavonoids, qualitative and quantitative analysis of phenolic compounds by liquid chromatography coupled to mass spectrometry and antibacterial activity against Escherichia coli, Staphylococcus spp, Streptococcus spp. It was found 34.39% and 13.46% of flavonoids and phenolic compounds, respectively, among which stand out ferulic acid (0.09 mg/g), caffeic acid (0.17 mg/g) and p-coumaric acid (3.39 mg/g). In the first 15 minutes incubation, all counts differed from the initial counts (p<0.01). With 15 minutes of exposure to the extract, Escherichia coli was inhibited by 38.72% and differed from the other bacterial genus (p<0.01, n=6). Staphylococcus spp. has had inhibition of 46.03% and Streptotococcus spp. of 50.8%. After 2 hours incubation, the genus Streptococcus was totally eliminated, Staphylococcus spp. after 3 hours and E. coli after 4 hours. Clearly, E. coli was more resistant bacteria than S. aureus. The other species of Staphylococcus and Streptococcus did not show large differences between the counts. We concluded that the composition of the brown propolis sample from Southern Brazil showed large levels of phenolic acids with proven antibacterial action, what explains the bactericidal activity found.Programa de Pós-Graduação em Veterinária Faculdade de Veterinária Universidade Federal de Pelotas (UFPel), Campus Universitário Capão do Leão s/nResidente em Saúde Coletiva Laboratório de Bacteriologia e Saúde Populacional UFPel, Campus Universitário Capão do Leão s/nPrograma de Pós-Graduação em Ciência e Tecnologia de Alimentos UFPel, Campus Universitário Capão do Leão s/nDepartamento de Higiene Veterinária e Saúde Pública Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista Júlio de Mesquita Filho, Campus Unesp/Botucatu, Distrito Rubiao Junior, s/nLaboratório de Bacteriologia e Saúde Populacional Departamento de Veterinária Preventiva Faculdade de Veterinária UFPel, Campus Universitário Capão do Leão s/nLaboratório de Virologia e Imunologia Departamento de Veterinária Preventiva Faculdade de Veterinária UFPel, Campus Universitário Capão do Leão s/nDepartamento de Higiene Veterinária e Saúde Pública Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista Júlio de Mesquita Filho, Campus Unesp/Botucatu, Distrito Rubiao Junior, s/nUniversidade Federal de Pernambuco (UFPE)Universidade Estadual Paulista (UNESP)Picoli, TonyPeter, Cristina MendesHoffmann, Jessica FernandaLatosinski, Giulia Soares [UNESP]Zani, João LuizVargas, Gilberto D'ÁvilaDe Oliveira Hübner, SilviaFischer, Geferson2022-04-29T08:05:44Z2022-04-29T08:05:44Z2016-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article365-371Revista Brasileira de Medicina Veterinaria, v. 38, n. 4, p. 365-371, 2016.2527-21790100-2430http://hdl.handle.net/11449/2283462-s2.0-85020913306Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Brasileira de Medicina Veterinariainfo:eu-repo/semantics/openAccess2022-04-29T08:05:44Zoai:repositorio.unesp.br:11449/228346Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:24:14.803841Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil Caracterização química e ação antibacteriana de extrato de própolis marrom da região sul do Brasil |
title |
Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil |
spellingShingle |
Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil Picoli, Tony Action time Antimicrobial Natural products |
title_short |
Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil |
title_full |
Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil |
title_fullStr |
Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil |
title_full_unstemmed |
Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil |
title_sort |
Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil |
author |
Picoli, Tony |
author_facet |
Picoli, Tony Peter, Cristina Mendes Hoffmann, Jessica Fernanda Latosinski, Giulia Soares [UNESP] Zani, João Luiz Vargas, Gilberto D'Ávila De Oliveira Hübner, Silvia Fischer, Geferson |
author_role |
author |
author2 |
Peter, Cristina Mendes Hoffmann, Jessica Fernanda Latosinski, Giulia Soares [UNESP] Zani, João Luiz Vargas, Gilberto D'Ávila De Oliveira Hübner, Silvia Fischer, Geferson |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Pernambuco (UFPE) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Picoli, Tony Peter, Cristina Mendes Hoffmann, Jessica Fernanda Latosinski, Giulia Soares [UNESP] Zani, João Luiz Vargas, Gilberto D'Ávila De Oliveira Hübner, Silvia Fischer, Geferson |
dc.subject.por.fl_str_mv |
Action time Antimicrobial Natural products |
topic |
Action time Antimicrobial Natural products |
description |
The aim of this study was to characterize chemically a sample of brown propolis and determine the time of action needed for eliminating microorganisms causing bovine mastitis. It was prepared hydroalcoholic extract of brown propolis (25 mg/mL) and this was evaluated for content of total phenolic compounds, total flavonoids, qualitative and quantitative analysis of phenolic compounds by liquid chromatography coupled to mass spectrometry and antibacterial activity against Escherichia coli, Staphylococcus spp, Streptococcus spp. It was found 34.39% and 13.46% of flavonoids and phenolic compounds, respectively, among which stand out ferulic acid (0.09 mg/g), caffeic acid (0.17 mg/g) and p-coumaric acid (3.39 mg/g). In the first 15 minutes incubation, all counts differed from the initial counts (p<0.01). With 15 minutes of exposure to the extract, Escherichia coli was inhibited by 38.72% and differed from the other bacterial genus (p<0.01, n=6). Staphylococcus spp. has had inhibition of 46.03% and Streptotococcus spp. of 50.8%. After 2 hours incubation, the genus Streptococcus was totally eliminated, Staphylococcus spp. after 3 hours and E. coli after 4 hours. Clearly, E. coli was more resistant bacteria than S. aureus. The other species of Staphylococcus and Streptococcus did not show large differences between the counts. We concluded that the composition of the brown propolis sample from Southern Brazil showed large levels of phenolic acids with proven antibacterial action, what explains the bactericidal activity found. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-01 2022-04-29T08:05:44Z 2022-04-29T08:05:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Brasileira de Medicina Veterinaria, v. 38, n. 4, p. 365-371, 2016. 2527-2179 0100-2430 http://hdl.handle.net/11449/228346 2-s2.0-85020913306 |
identifier_str_mv |
Revista Brasileira de Medicina Veterinaria, v. 38, n. 4, p. 365-371, 2016. 2527-2179 0100-2430 2-s2.0-85020913306 |
url |
http://hdl.handle.net/11449/228346 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Revista Brasileira de Medicina Veterinaria |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
365-371 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128804982358016 |